MicroRNA-30a Inhibits Colorectal Cancer Metastasis Through Down-Regulation of Type I Insulin-Like Growth Factor Receptor.

Abstract:

BACKGROUND:miR-30a expression is down-regulated and regulates tumor suppressors in various cancers. AIM:We investigated the mechanisms underlying the biological role of miR-30a in CRC. METHODS:MicroRNA, mRNA, and protein expression were analyzed by quantitative real-time PCR and Western blot. The migration and invasion abilities of CRC were determined by wound healing assay, and trans-well migration and invasion. A luciferase reporter assay was used to confirm the targets of miR-30a. RESULTS:miR-30a expression was significantly down-regulated in CRC tissues and in CRC tissue with lymph node metastasis compared to CRC tissue without metastasis. Overexpression of miR-30a suppressed migration and invasion through insulin-like growth factor 1 receptor (IGF1R) in CRC cells. miR-30a suppresses IGF1R protein expression and inhibits β-catenin or p-AKT and increases E-cadherin expression. The IGF1R expression level is also up-regulated in CRC tumors and inversely correlated with miR-30a in CRC specimens. CONCLUSIONS:miR-30a functions as a tumor-suppressive miRNA, which may provide a therapeutic strategy for metastasis of CRC.

journal_name

Dig Dis Sci

authors

Liu YC,Park YR,Kim SL,Lee ST,Kim SW

doi

10.1007/s10620-017-4763-z

subject

Has Abstract

pub_date

2017-11-01 00:00:00

pages

3040-3049

issue

11

eissn

0163-2116

issn

1573-2568

pii

10.1007/s10620-017-4763-z

journal_volume

62

pub_type

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