Abstract：
AIMS:Asiaticoside (AS) is a saponin monomer extracted from the medicinal plant Centella asiatica, which has a variety of biological effects. We intended to investigate the effects of asiaticoside on a hypoxia-induced pulmonary hypertension (HPH) rat model and examine the possible effects of asiaticoside on TGF-β1/Smad signaling in vivo and in vitro. MAIN METHODS:The rat HPH model was established by hypoxic exposure and asiaticoside was administered for four weeks. Parameters including the mean pulmonary artery pressure (mPAP), the right ventricular hypertrophy (RVH) and the percentage of medial wall thickness were used to evaluate the development of HPH. TGF-β1, TGF-β receptor, Smad2/3 and phospho-Smad2/3 expressions were detected and the proliferation, migration and apoptosis of pulmonary arterial smooth muscle cells (PASMCs) adjusted by asiaticoside under the hypoxic condition were evaluated. KEY FINDINGS:Our data indicate that asiaticoside attenuated pulmonary hypertension, pulmonary vascular remodeling and RV hypertrophy in HPH rats, which was probably mediated by restraining the hypoxia-induced overactive TGF-β1/Smad2/3 signaling and inhibiting the proliferation by inducing apoptosis of the PASMCs. SIGNIFICANCE:Given the preventative potential in animal models and in vitro, we propose asiaticoside as a promising protective treatment in HPH.
journal_name
Life Scijournal_title
Life sciencesauthors
Wang XB,Wang W,Zhu XC,Ye WJ,Cai H,Wu PL,Huang XY,Wang LXdoi
10.1016/j.lfs.2015.07.016subject
Has Abstractpub_date
2015-09-15 00:00:00pages
56-64eissn
0024-3205issn
1879-0631pii
S0024-3205(15)00368-9journal_volume
137pub_type
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