Abstract：
:Left-right (LR) asymmetry is a biologically conserved property in living organisms that can be observed in the asymmetrical arrangement of organs and tissues and in tissue morphogenesis, such as the directional looping of the gastrointestinal tract and heart. The expression of LR asymmetry in embryonic tissues can be appreciated in biased cell alignment. Previously an in vitro chirality assay was reported by patterning multiple cells on microscale defined geometries and quantified the cell phenotype-dependent LR asymmetry, or cell chirality. However, morphology and chirality of individual cells on micropatterned surfaces has not been well characterized. Here, a Python-based algorithm was developed to identify and quantify immunofluorescence stained individual epithelial cells on multicellular patterns. This approach not only produces results similar to the image intensity gradient-based method reported previously, but also can capture properties of single cells such as area and aspect ratio. We also found that cell nuclei exhibited biased alignment. Around 35% cells were misaligned and were typically smaller and less elongated. This new imaging analysis approach is an effective tool for measuring single cell chirality inside multicellular structures and can potentially help unveil biophysical mechanisms underlying cellular chiral bias both in vitro and in vivo.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Raymond MJ Jr,Ray P,Kaur G,Singh AV,Wan LQdoi
10.1007/s10439-015-1431-3subject
Has Abstractpub_date
2016-05-01 00:00:00pages
1475-86issue
5eissn
0090-6964issn
1573-9686pii
10.1007/s10439-015-1431-3journal_volume
44pub_type
杂志文章abstract：:Crowding can substantially affect the transition of a protein between its native (N) and unfolded (U) states via volume exclusion effects. Also, it influences considerably the aggregation (A) of unfolded proteins. To examine the details, we developed an approach for computing the kinetic rates of the process N <--> U ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-5780-8
更新日期：2005-08-01 00:00:00
abstract：:Vascular smooth muscle mechanics have been studied in vitro in cylindrical segments of dog carotid artery, human internal mammary artery, and human saphenous vein. Such cylindrical preparations maintain normal vessel geometry and also permit correlation of mechanical phenomena with transmural pressure. These studies s...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02363919
更新日期：1984-01-01 00:00:00
abstract：:A novel apparatus for the multifaceted evaluation of artery function was developed. It measures endothelial and smooth muscle functions and the pressure-strain elastic modulus (E p). A rigid airtight chamber with an ultrasound probe was attached to the upper arm to manipulate the transmural pressure of the brachial ar...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-017-1810-z
更新日期：2017-06-01 00:00:00
abstract：:Nanomedicine (a division of nanotechnology) is an interdisciplinary research field incorporating biology, chemistry, engineering and medicine with the intention to improve disease prevention, diagnosis, and treatment. Specifically, there have been great strides made in using nanomedicine to enhance the functions of ce...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1007/s10439-009-9722-1
更新日期：2009-10-01 00:00:00
abstract：:Early stage changes in hypertensive arteries have a significant effect on the long-term adaptation of the arteries. Compared to the long-term adaptation, little is known about the early dimensional and functional changes in hypertensive arteries in the first few days of hypertension. To study the early stage changes i...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1376391
更新日期：2001-06-01 00:00:00
abstract：:Spine musculoskeletal models used to estimate loads and displacements require many simplifying assumptions. We examined how assumptions about subject size and vertebral positions can affect the model outcomes. Head and neck models were developed to represent 30 subjects (15 males and 15 females) in neutral posture and...
journal_title：Annals of biomedical engineering
pub_type： 临床试验,杂志文章
doi：10.1007/s10439-018-2084-9
更新日期：2018-11-01 00:00:00
abstract：:Dexterity of robots is highly required when it comes to integration for medical applications. Major efforts have been conducted to increase the dexterity at the distal parts of medical robots. This paper reports on developments toward integrating biocompatible conducting polymers (CP) into inherently dexterous concent...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-018-2038-2
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND:Common-mode noise degrades cardiovascular signal quality and diminishes measurement accuracy. Filtering to remove noise components in the frequency domain often distorts the signal. METHOD:Two adaptive noise canceling (ANC) algorithms were tested to adjust weighted reference signals for optimal subtraction ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-007-9294-x
更新日期：2007-07-01 00:00:00
abstract：:Traditionally, measurement of pulmonary O2 uptake uses mass balance of N2 to correct for differences between inspired and expired volume (V) due to temperature (T) and relative humidity (RH). Often during anesthesia, N2 balance cannot be invoked due to high inspired O2 fraction (FIO2) or nonsteady state conditions. Th...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1312184
更新日期：2000-09-01 00:00:00
abstract：:We propose a multiphysical mathematical model by fully coupling lipid deposition, monocytes/macrophages recruitment and angiogenesis to investigate the pathophysiological responses of an atherosclerotic plaque to the dynamic changes in the microenvironment. The time evolutions of cellular (endothelial cells, macrophag...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-018-02173-1
更新日期：2019-02-01 00:00:00
abstract：:Phenotype transformation of vascular smooth muscle cells (VSMCs) has been reported to be directly influenced by the frequency of mechanical strain. This study explored the effects of different frequencies of mechanical strain on expression of phenotype marker h1-calponin and the possible mechanism. VSMCs were subjecte...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-008-9521-0
更新日期：2008-09-01 00:00:00
abstract：:Additive manufacturing, also known as 3D printing, has emerged over the past 3 decades as a disruptive technology for rapid prototyping and manufacturing. Vat polymerization, powder bed fusion, material extrusion, and binder jetting are distinct technologies of additive manufacturing, which have been used in a wide va...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1007/s10439-016-1678-3
更新日期：2017-01-01 00:00:00
abstract：:In this paper, we present the application of a semi-global inverse method for determining material parameters of biological tissues. The approach is based on the successive response surface method, and is illustrated by fitting constitutive parameters to two nonlinear anisotropic constitutive equations, one for aortic...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-8338-3
更新日期：2005-12-01 00:00:00
abstract：:Computational modeling and simulation has become more common in design and development of bioprosthetic heart valves. To have a reliable computational model, considering accurate mechanical properties of biological soft tissue is one of the most important steps. The goal of this study was to present a non-invasive mat...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-018-02129-5
更新日期：2019-01-01 00:00:00
abstract：:Deep tissue injury (DTI) is a type of pressure ulcer in which tissue breakdown initiates at the bone-muscle interface under intact skin. Excessive deformation in the soft tissue, particularly around bony prominences, is believed to be one of the causes leading to the development of DTI. The main goal of this study was...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-012-0539-y
更新日期：2012-08-01 00:00:00
abstract：:This project was undertaken to establish if the integral circulation had any effects upon the mechanical behavior of bone. The influence of blood flow in the rabbit tibia on the strain induced at the bone surface when under load was assessed. Recordings were taken from three adult and three immature New Zealand White ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02390377
更新日期：1994-03-01 00:00:00
abstract：:Measurement of the instantaneous pressure-volume relationship of the left ventricle is fundamental to the study of ventricular mechanics. In order to effectively investigate this relationship, it is necessary to vary and control the time course of ventricular volume change in a variety of prescribed manners. In the pa...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02367387
更新日期：1982-01-01 00:00:00
abstract：:Quantifying injury tolerance for concussion is complicated by variability in the type, severity, and time course of post-injury physiological and behavioral changes. The current study outlined acute and chronic changes in behavioral metrics following rotational acceleration-induced concussion in rats. The Medical Coll...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1647-x
更新日期：2016-11-01 00:00:00
abstract：:In this study, we introduce the fast wavelet transform as a method for characterizing maturational changes in electrocortical activity, respiratory activity, and blood pressure in fetal lambs in early (110-122 days), mid (123-135 days), and late (136-144 days) third trimester (term 145 days). Each recording was 2 hr i...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02584457
更新日期：1995-09-01 00:00:00
abstract：:A nonintrusive method for the visualization of pulsatile flow velocity profiles is described. The method is based on the use of a photochromic dye that is added to the fluid being studied and a nitrogen laser which excites the dye producing a marker "line" whose movement can be photographed. A microcomputer is used as...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02584271
更新日期：1986-01-01 00:00:00
abstract：:This paper discusses the dynamics of the circus movement around a one-dimensional continuous and uniform loop of model cardiac cells. The membrane ionic currents are represented by a modified Beeler-Reuter formulation. The description of the quasiperiodic regimes initiated in a previous paper is completed and their eq...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1289458
更新日期：2000-07-01 00:00:00
abstract：:Simulations are useful to study the heart's ability to generate flow and the interaction between contractility and loading conditions. The left ventricular pressure-volume (PV) relation has been shown to be nonlinear, but it is unknown whether a linear model is accurate enough for simulations. Six models were fitted t...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-009-9742-x
更新日期：2009-09-01 00:00:00
abstract：:Proper replacement or repair of damaged tendons or ligaments requires functionally engineered tissue that mimics their native mechanical properties. While tendon structure-function relationships are generally assumed, there exists little quantitative evidence of the roles of distinct tendon components in tendon functi...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1023/b:abme.0000032455.78459.56
更新日期：2004-07-01 00:00:00
abstract：:Gap junctions play a fundamental role in intercellular communication in cardiac tissue. Various types of heart disease including hypertrophy and ischemia are associated with alterations of the spatial arrangement of gap junctions. Previous studies applied two-dimensional optical and electron-microscopy to visualize ga...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-011-0369-3
更新日期：2011-11-01 00:00:00
abstract：:Biologically active heart valves (tissue engineered and recellularized tissue-derived heart valves) have the potential to offer enhanced function when compared to current replacement value therapies since they can possibly remodel, and grow to meet the needs of the patient, and not require chronic medication. However,...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/b:abme.0000036640.11387.4b
更新日期：2004-08-01 00:00:00
abstract：:While bone mineral density has been traditionally used to quantify fracture risk for individuals with spinal cord injuries, recent studies are including engineering measurements such as section modulus and cross sectional moment of inertia. These are almost exclusively calculated by peripheral QCT scanners which, unli...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-017-1796-6
更新日期：2017-05-01 00:00:00
abstract：:In this study various blood rheological assumptions are numerically investigated for the hemodynamic properties of intra-stent flow. Non-newtonian blood properties have never been implemented in blood coronary stented flow investigation, although its effects appear essential for a correct estimation and distribution o...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-006-9123-7
更新日期：2006-08-01 00:00:00
abstract：:Recently, we reported that tethering and rolling of neutrophils in shear flow over a substrate of E-selectin signals activation of beta 2-integrins and firm adhesion via an intracellular signaling pathway involving phosphorylation of p38 MAP kinase. In the current study the objective was to examine the molecular mecha...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1511240
更新日期：2002-09-01 00:00:00
abstract：:Potential human and environmental hazards resulting from the exposure of living organisms to silver nanoparticles (Ag NPs) have been the subject of intensive discussion in the last decade. Despite the growing use of Ag NPs in biomedical applications, a quantification of the toxic effects as a function of the total sil...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02449-5
更新日期：2020-04-01 00:00:00
abstract：:For treatment of complex congenital heart disease, computer simulation using a three-dimensional heart model may help to improve outcomes by enabling detailed preoperative evaluations. However, no highly integrated model that accurately reproduces a patient's pathophysiology, which is required for this simulation has ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02488-y
更新日期：2020-06-01 00:00:00
