Non-linear kinetics of glutamyl-tRNA synthesis catalyzed by high molecular weight complexes from rat brain neuronal cells but not from glial cells.

Abstract:

:High molecular weight complexes of aminoacyl-tRNA synthetases isolated from rat brain catalyze the formation of glutamyl-tRNA with an initial lag time of the order of 1 min, as previously reported for the formation of glutamyl-tRNA and glutaminyl-tRNA catalyzed by similar complexes from bovine brain (Vadeboncoeur and Lapointe, Eur. J. Biochem., 109 (1980) 581-587). To determine the type(s) of brain cell(s) where this phenomenon occurs, we have studied the kinetics of glutamyl-tRNA formation catalyzed by high molecular weight complexes of aminoacyl-tRNA synthetases isolated from neuronal and from glial cells, either transformed (Neuro-2A and C6), or from primary cultures, or isolated from rat brain. The delay in the formation of glutamyl-tRNA was observed only in the case of neuronal cells isolated from rat brain, whereas a delay in the formation of glutaminyl-tRNA was also seen in these cells, as well as in neuronal cells in primary culture and in synaptosomes. The kinetics of formation of aspartyl-tRNA and valyl-tRNA catalyzed by high molecular weight complexes from all these cells was linear.

journal_name

Brain Res

journal_title

Brain research

authors

Lacoste L,Hertz L,Lapointe J

doi

10.1016/0169-328x(86)90016-1

subject

Has Abstract

pub_date

1986-07-01 00:00:00

pages

17-27

issue

1

eissn

0006-8993

issn

1872-6240

journal_volume

387

pub_type

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