Posterior talar process as a suitable cell source for treatment of cartilage and osteochondral defects of the talus.

Abstract:

:Osteochondral defects of the ankle are common lesions affecting the talar cartilage and subchondral bone. Current treatments include cell-based therapies but are frequently associated with donor-site morbidity. Our objective is to characterize the posterior process of the talus (SP) and the os trigonum (OT) tissues and investigate their potential as a new source of viable cells for application in tissue engineering and regenerative medicine. SP and OT tissues obtained from six patients were characterized by micro-computed tomography and histological, histomorphometric and immunohistochemical analyses. Proliferation and viability of isolated cells were evaluated by MTS assay, DNA quantification and live/dead staining. The TUNEL assay was performed to evaluate cell death by apoptosis. Moreover, the production of extracellular matrix was evaluated by toluidine blue staining, whereas cells phenotype was investigated by flow cytometry. Characterization of ankle explants showed the presence of a cartilage tissue layer in both SP and OT tissues, which represented at least 20%, on average, of the explant. The presence of type II collagen was detected in the extracellular matrix. Isolated cells presented a round morphology typical of chondrocytes. In in vitro studies, cells were viable and proliferating for up to 21 days of culture. No signs of apoptosis were detected. Flow-cytometry analysis revealed that isolated cells maintained the expression of several chondrocytic markers during culture. The results indicated that the SP and OT tissues were a reliable source of viable chondrocytes, which could find promising applications in ACI/MACI strategies with minimal concerns regarding donor zone complications. Copyright © 2015 John Wiley & Sons, Ltd.

journal_name

J Tissue Eng Regen Med

authors

Correia SI,Silva-Correia J,Pereira H,Canadas RF,da Silva Morais A,Frias AM,Sousa RA,van Dijk CN,Espregueira-Mendes J,Reis RL,Oliveira JM

doi

10.1002/term.2092

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

1949-1962

issue

7

eissn

1932-6254

issn

1932-7005

journal_volume

11

pub_type

杂志文章
  • Potential induction of rat muscle-derived stem cells to neural-like cells by retinoic acid.

    abstract::Several recent studies have demonstrated that stem cell differentiation can be generated by derivatives of retinoic acid. In this study we chose retinoic acid (RA) for inducing neural differentiation of rat muscle-derived stem cells (rMDSCs). rMDSCs were pre-induced with 10 ng/ml basic fibroblast growth factor (bFGF) ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.328

    authors: Kim ES,Kim GH,Kang ML,Kang YM,Kang KN,Hwang KC,Min BH,Kim JH,Kim MS

    更新日期:2011-05-01 00:00:00

  • Vibration stimulates vocal mucosa-like matrix expression by hydrogel-encapsulated fibroblasts.

    abstract::The composition and organization of the vocal fold extracellular matrix (ECM) provide the viscoelastic mechanical properties that are required to sustain high-frequency vibration during voice production. Although vocal injury and pathology are known to produce alterations in matrix physiology, the mechanisms responsib...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.219

    authors: Kutty JK,Webb K

    更新日期:2010-01-01 00:00:00

  • Fabrication and evaluation of carbonate apatite-coated calcium carbonate bone substitutes for bone tissue engineering.

    abstract::Carbonate apatite-coated calcium carbonate (CO3 Ap/CaCO3 ) was fabricated through a dissolution-precipitation reaction using CaCO3 granules as a precursor to accelerate bone replacement based on superior osteoconductivity of the CO3 Ap shell, along with Ca2+ release from the CaCO3 core and quicker resorption of the Ca...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2742

    authors: Fujioka-Kobayashi M,Tsuru K,Nagai H,Fujisawa K,Kudoh T,Ohe G,Ishikawa K,Miyamoto Y

    更新日期:2018-10-01 00:00:00

  • In vitro and in vivo enhancement of osteogenic capacity in a synthetic BMP-2 derived peptide-coated mineralized collagen composite.

    abstract::Enhancement of osteogenic capacity was achieved in a mineralized collagen composite, nano-hydroxyapatite/collagen (nHAC), by loading with synthetic peptides derived from BMP-2 residues 32-48 (P17-BMP-2). Rabbit marrow stromal cells (MSCs) were used in vitro to study cell biocompatibility, attachment and differentiatio...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1705

    authors: Zhang X,Guo WG,Cui H,Liu HY,Zhang Y,Müller WE,Cui FZ

    更新日期:2016-02-01 00:00:00

  • Improvement of the in vivo cellular repopulation of decellularized cardiovascular tissues by a detergent-free, non-proteolytic, actin-disassembling regimen.

    abstract::Low immunogenicity and high repopulation capacity are crucial determinants for the functional and structural performance of acellular cardiovascular implants. The present study evaluates a detergent-free, non-proteolytic, actin-disassembling regimen (BIO) for decellularization of heart valve and vessel grafts, particu...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2271

    authors: Assmann A,Struß M,Schiffer F,Heidelberg F,Munakata H,Timchenko EV,Timchenko PE,Kaufmann T,Huynh K,Sugimura Y,Leidl Q,Pinto A,Stoldt VR,Lichtenberg A,Akhyari P

    更新日期:2017-12-01 00:00:00

  • Initial evaluation of vascular ingrowth into superporous hydrogels.

    abstract::There is a need for new materials and architectures for tissue engineering and regenerative medicine. Based upon our recent results developing novel scaffold architecture, we hypothesized that this new architecture would foster vascularization, a particular need for tissue engineering. We report on the potential of su...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.183

    authors: Keskar V,Gandhi M,Gemeinhart EJ,Gemeinhart RA

    更新日期:2009-08-01 00:00:00

  • The future of heart valve replacement: recent developments and translational challenges for heart valve tissue engineering.

    abstract::Heart valve replacement is often the only solution for patients suffering from valvular heart disease. However, currently available valve replacements require either life-long anticoagulation or are associated with valve degeneration and calcification. Moreover, they are suboptimal for young patients, because they do ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.2326

    authors: Fioretta ES,Dijkman PE,Emmert MY,Hoerstrup SP

    更新日期:2018-01-01 00:00:00

  • Fetal subcutaneous cells have potential for autologous tissue engineering.

    abstract::Major congenital malformations affect up to 3% of newborns. Infants with prenatally diagnosed soft tissue defects should benefit from having autologous tissue readily available for surgical implantation in the perinatal period. In this study, we investigate fetal subcutaneous cells as cellular source for tissue engine...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2639

    authors: Ekblad Å,Westgren M,Fossum M,Götherström C

    更新日期:2018-05-01 00:00:00

  • Optimal timing and frequency of bone marrow soup therapy for functional restoration of salivary glands injured by single-dose or fractionated irradiation.

    abstract::Injections of bone marrow (BM) cell extract, known as 'BM soup', were previously reported to mitigate ionizing radiation (IR) injury to salivary glands (SGs). However, the optimal starting time and frequency to maintain BM soup therapeutic efficacy remains unknown. This study tested the optimal starting time and frequ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2513

    authors: Fang D,Shang S,Liu Y,Bakkar M,Sumita Y,Seuntjens J,Tran SD

    更新日期:2018-02-01 00:00:00

  • Current advances in the generation of human iPS cells: implications in cell-based regenerative medicine.

    abstract::Over the last few years, the generation of induced pluripotent stem cells (iPSCs) from human somatic cells has proved to be one of the most potentially useful discoveries in regenerative medicine. iPSCs are becoming an invaluable tool to study the pathology of different diseases and for drug screening. However, severa...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.2021

    authors: Revilla A,González C,Iriondo A,Fernández B,Prieto C,Marín C,Liste I

    更新日期:2016-11-01 00:00:00

  • Kartogenin with PRP promotes the formation of fibrocartilage zone in the tendon-bone interface.

    abstract::Treatment of tendon-bone junction injuries is a challenge because tendon-bone interface often heals poorly and the fibrocartilage zone, which reduces stress concentration, at the interface is not formed. In this study, we used a compound called kartogenin (KGN) with platelet-rich plasma (PRP) to induce the formation o...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2258

    authors: Zhou Y,Zhang J,Yang J,Narava M,Zhao G,Yuan T,Wu H,Zheng N,Hogan MV,Wang JH

    更新日期:2017-12-01 00:00:00

  • Ibuprofen-loaded poly(trimethylene carbonate-co-ε-caprolactone) electrospun fibres for nerve regeneration.

    abstract::The development of scaffolds that combine the delivery of drugs with the physical support provided by electrospun fibres holds great potential in the field of nerve regeneration. Here it is proposed the incorporation of ibuprofen, a well-known non-steroidal anti-inflammatory drug, in electrospun fibres of the statisti...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1792

    authors: Pires LR,Guarino V,Oliveira MJ,Ribeiro CC,Barbosa MA,Ambrosio L,Pêgo AP

    更新日期:2016-03-01 00:00:00

  • Melatonin promotes survival of nonvascularized fat grafts and enhances the viability and migration of human adipose-derived stem cells via down-regulation of acute inflammatory cytokines.

    abstract::Nonvascularized fat grafting is a valuable technique for soft tissue reconstruction but poor survival of fat in the host environment remains a problem. A process known as cell-assisted transfer is used to enhance fat graft retention by adding stromal vascular fraction, an adipose-derived stem cell (ASC) rich content t...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2463

    authors: Tan SS,Zhan W,Poon CJ,Han X,Marre D,Boodhun S,Palmer JA,Mitchell GM,Morrison WA

    更新日期:2018-02-01 00:00:00

  • Morphological transformation of hBMSC from 2D monolayer to 3D microtissue on low-crystallinity SF-PCL patch with promotion of cardiomyogenesis.

    abstract::The effects of the stiffness of substrates on the cell behaviours of human bone marrow-derived mesenchymal stem cells (hBMSC) have been investigated, but the effects of the secondary structures of proteins in the substrates on the morphological transformation and differentiation of hBMSC have yet been elucidated. To i...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2616

    authors: Lo HY,Huang AL,Lee PC,Chung TW,Wang SS

    更新日期:2018-04-01 00:00:00

  • Poly(ester-urethane) scaffolds: effect of structure on properties and osteogenic activity of stem cells.

    abstract::The present study aimed to investigate the effect of structure (design and porosity) on the matrix stiffness and osteogenic activity of stem cells cultured on poly(ester-urethane) (PEU) scaffolds. Different three-dimensional (3D) forms of scaffold were prepared from lysine-based PEU using traditional salt-leaching and...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1848

    authors: Kiziltay A,Marcos-Fernandez A,San Roman J,Sousa RA,Reis RL,Hasirci V,Hasirci N

    更新日期:2015-08-01 00:00:00

  • Human chondroprogenitors in alginate-collagen hybrid scaffolds produce stable cartilage in vivo.

    abstract::The goal of this study was to evaluate human epiphyseal chondroprogenitor cells (ECPs) as a potential new cell source for cartilage regeneration. ECPs were compared to human bone marrow stromal cells (MSCs) and human adult articular chondrocytes (ACs) for their chondrogenic potential and phenotypic stability in vitro ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2203

    authors: Studer D,Cavalli E,Formica FA,Kuhn GA,Salzmann G,Mumme M,Steinwachs MR,Laurent-Applegate LA,Maniura-Weber K,Zenobi-Wong M

    更新日期:2017-11-01 00:00:00

  • Phenotype-based selection of bone marrow mesenchymal stem cell-derived smooth muscle cells for elastic matrix regenerative repair in abdominal aortic aneurysms.

    abstract::Chronic proteolytic disruption of elastic fibres within the abdominal aortic wall results in wall vessel expansion to form rupture-prone abdominal aortic aneurysms (AAA). Arresting AAA growth is not possible as adult vascular smooth muscle cells (SMCs) poorly auto-regenerate and repair elastic fibres. Thus, there is a...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2349

    authors: Swaminathan G,Stoilov I,Broekelmann T,Mecham R,Ramamurthi A

    更新日期:2018-01-01 00:00:00

  • Anti-angiogenic potential of VEGF blocker dendron loaded on to gellan gum hydrogels for tissue engineering applications.

    abstract::Damage of non-vascularised tissues such as cartilage and cornea can result in healing processes accompanied by a non-physiological angiogenesis. Peptidic aptamers have recently been reported to block the vascular endothelial growth factor (VEGF). However, the therapeutic applications of these aptamers are limited due ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2340

    authors: Perugini V,Guildford AL,Silva-Correia J,Oliveira JM,Meikle ST,Reis RL,Santin M

    更新日期:2018-02-01 00:00:00

  • Hybrid PGS-PCL microfibrous scaffolds with improved mechanical and biological properties.

    abstract::Poly(glycerol sebacate) (PGS) is a biodegradable elastomer that has generated great interest as a scaffold material due to its desirable mechanical properties. However, the use of PGS in tissue engineering is limited by difficulties in casting micro- and nanofibrous structures, due to high temperatures and vacuum requ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.313

    authors: Sant S,Hwang CM,Lee SH,Khademhosseini A

    更新日期:2011-04-01 00:00:00

  • New observations of the hierarchical structure of human enamel, from nanoscale to microscale.

    abstract::Microstructure in terms of hierarchical assembly exists widely in mineralized biomaterials, fulfilling an important role in setting up their outstanding properties. The purpose of this study was to investigate the hierarchical assembly of enamel structure and functions, which are related to the unique characteristics ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.21

    authors: Cui FZ,Ge J

    更新日期:2007-05-01 00:00:00

  • Bone tissue engineering in oral peri-implant defects in preclinical in vivo research: A systematic review and meta-analysis.

    abstract::The regeneration and establishment of osseointegration within oral peri-implant bone defects remains a clinical challenge. Bone tissue engineering (BTE) is emerging as a promising alternative to autogenous and/or biomaterial-based bone grafting. The objective of this systematic review was to answer the focused questio...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,meta分析,评审

    doi:10.1002/term.2412

    authors: Shanbhag S,Pandis N,Mustafa K,Nyengaard JR,Stavropoulos A

    更新日期:2018-01-01 00:00:00

  • Human osteoarthritic chondrons outnumber patient- and joint-matched chondrocytes in hydrogel culture-Future application in autologous cell-based OA cartilage repair?

    abstract::Autologous chondrocyte implantation (ACI) is used in 34-60% for osteoarthritic (OA) cartilage defects, although ACI is neither recommended nor designed for OA. Envisioning a hydrogel-based ACI for OA that uses chondrons instead of classically used chondrocytes, we hypothesized that human OA chondrons may outperform OA...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2516

    authors: Rothdiener M,Uynuk-Ool T,Südkamp N,Aurich M,Grodzinsky AJ,Kurz B,Rolauffs B

    更新日期:2018-02-01 00:00:00

  • Novel wound dressing based on nanofibrous PHBV-keratin mats.

    abstract::Keratin is an important protein used for wound healing and tissue recovery. In this study, keratin was first extracted from raw materials and chemically modified to obtain stable keratin (m-keratin). The raw and m-keratin were examined by Raman spectroscopy. The molecular weight of the m-keratin was analysed by SDS-PA...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1653

    authors: Yuan J,Geng J,Xing Z,Shim KJ,Han I,Kim JC,Kang IK,Shen J

    更新日期:2015-09-01 00:00:00

  • Progenitor and stem cells for bone and cartilage regeneration.

    abstract::Research in regenerative medicine is developing at a significantly quick pace. Cell-based bone and cartilage replacement is an evolving therapy aiming at the treatment of patients who suffer from limb amputation, damaged tissues and various bone and cartilage-related disorders. Stem cells are undifferentiated cells wi...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.173

    authors: El Tamer MK,Reis RL

    更新日期:2009-07-01 00:00:00

  • Biological performance of cell-encapsulated methacrylated gellan gum-based hydrogels for nucleus pulposus regeneration.

    abstract::Limitations of current treatments for intervertebral disc (IVD) degeneration have promoted interest in the development of tissue-engineering approaches. Injectable hydrogels loaded with cells can be used as a substitute material for the inner IVD part, the nucleus pulposus (NP), and provide an opportunity for minimall...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1959

    authors: Tsaryk R,Silva-Correia J,Oliveira JM,Unger RE,Landes C,Brochhausen C,Ghanaati S,Reis RL,Kirkpatrick CJ

    更新日期:2017-03-01 00:00:00

  • Flow-perfusion bioreactor system for engineered breast cancer surrogates to be used in preclinical testing.

    abstract::There is a need for preclinical testing systems that predict the efficacy, safety and pharmacokinetics of cancer therapies better than existing in vitro and in vivo animal models. An approach to the development of predictive in vitro systems is to more closely recapitulate the cellular and spatial complexity of human ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2026

    authors: Marshall LE,Goliwas KF,Miller LM,Penman AD,Frost AR,Berry JL

    更新日期:2017-04-01 00:00:00

  • Human periosteum-derived cells from elderly patients as a source for cartilage tissue engineering?

    abstract::The aim of this study was to establish the potential of human periosteum-derived cells from elderly patients as a cell source for cartilage tissue engineering by optimizing culture conditions for both proliferation and differentiation. Periosteum was obtained from the tibiae of nine patients. Biopsies were prepared fo...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.100

    authors: Jansen EJ,Emans PJ,Guldemond NA,van Rhijn LW,Welting TJ,Bulstra SK,Kuijer R

    更新日期:2008-08-01 00:00:00

  • Dendrimers: Breaking the paradigm of current musculoskeletal autoimmune therapies.

    abstract::The advances achieved by cell-based therapies to treat autoimmune diseases such as rheumatoid arthritis (RA) and multiple sclerosis (MS), despite promising, are still insufficient for the current demands. RA and MS therapeutic approaches follow world guidelines to use disease modifying drugs and biological agents that...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.2597

    authors: Rodrigues DB,Oliveira JM,Santos TC,Reis RL

    更新日期:2018-03-01 00:00:00

  • Characterizing differences between MSCs and TM cells: Toward autologous stem cell therapies for the glaucomatous trabecular meshwork.

    abstract::Glaucoma, a leading cause of blindness, is characterized by an increase in intraocular pressure, which is largely determined by resistance to aqueous humour outflow through the trabecular meshwork (TM). In glaucoma, the cellularity of the TM is decreased, and, as a result, stem cell therapies for the TM represent a po...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2488

    authors: Snider EJ,Vannatta RT,Schildmeyer L,Stamer WD,Ethier CR

    更新日期:2018-03-01 00:00:00

  • Cyclic tension promotes fibroblastic differentiation of human MSCs cultured on collagen-fibre scaffolds.

    abstract::Mesenchymal stem cells (MSCs) have been suggested as a potential cell source for tendon/ligament tissue engineering. Extrinsic cues, such as the chemical and physical properties of scaffolds, as well as external forces, play an important role in fibroblastic differentiation of these cells. In this study, we employed a...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1880

    authors: Qiu Y,Lei J,Koob TJ,Temenoff JS

    更新日期:2016-12-01 00:00:00