T cell depletion of human bone marrow. Comparison of Campath-1 plus complement, anti-T cell ricin A chain immunotoxin, and soybean agglutinin alone or in combination with sheep erythrocytes or immunomagnetic beads.

Abstract:

:The aim of this study was to compare the extent of in vitro T cell depletion and recovery of hematopoietic progenitor cells achieved with five methods of T cell depletion. Bone marrow samples from the same source were treated with monoclonal antibody Campath-1 (CP1) and human complement, XomaZyme-H65 (anti-T cell ricin A chain immunotoxin), or soybean agglutinin (SBA) alone or in combination with sheep erythrocytes (EAET) or a cocktail of immunomagnetic beads (B) directly coated with anti-CD2, anti-CD3, or anti-CD8 monoclonal antibodies. Residual T cells were enumerated by limiting dilution analysis, EAET rosetting, and proliferative responses to phytohemagglutinin. The results of this study demonstrated the following reductions in BM T cells as detected by limiting dilution analysis (mean % control): SBA+B (99.9%), SBA+EAET (99.8%), CP1+C' (99.4%), anti-T cell ricin A chain immunotoxin (99.0%), and SBA alone (94.2%). Neither PHA response nor enumeration of residual EAET rosettes provided discriminating differences in the degree of T cell depletion by treatment method when T cell reductions exceeded 99.0% by LDA. These results demonstrate the ability of CP1+C', XomaZyme-H65, and SBA plus sheep erythrocyte or magnetic bead depletion to achieve a greater than 99% reduction of BM T cells and the importance of limiting dilution analysis in defining differences in T cell numbers when depletion exceeded 99%.

journal_name

Transplantation

journal_title

Transplantation

authors

Frame JN,Collins NH,Cartagena T,Waldmann H,O'Reilly RJ,Dupont B,Kernan NA

doi

10.1097/00007890-198906000-00013

subject

Has Abstract

pub_date

1989-06-01 00:00:00

pages

984-8

issue

6

eissn

0041-1337

issn

1534-6080

journal_volume

47

pub_type

杂志文章
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    abstract:BACKGROUND:Deceased donor kidneys with acute kidney injury (AKI) and deceased after circulatory death kidneys are viable sources of organs. The outcomes of renal transplantation from deceased after circulatory death (DCD) donors with AKI are not known. METHODS:A retrospective review of deceased donor renal transplants...

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    doi:10.1097/TP.0000000000003317

    authors: Lia D,Singer P,Nair V,Yang J,Teperman L,Grodstein E

    更新日期:2020-05-15 00:00:00

  • Bcl-2 protection of islet allografts is unmasked by costimulation blockade.

    abstract::One major limitation in pancreatic islet transplantation is availability of donor tissue. Donor shortage is exacerbated by islet apoptosis from the stresses of islet isolation and transplantation. Furthermore, the side effects of immunosuppressive drugs preclude transplants into patients whose diabetes is controlled b...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000132283.95107.9c

    authors: Sutherland RM,Allison J,Thomas HE,Brady JL,Kay TW,Lew AM

    更新日期:2004-05-27 00:00:00

  • Critical influence of natural regulatory CD25+ T cells on the fate of allografts in the absence of immunosuppression.

    abstract:BACKGROUND:Allografts are occasionally accepted in the absence of immunosuppression. Because naturally occurring CD4(+)CD25(+) regulatory T cells (natural CD25(+) Treg cells) have been shown to inhibit allograft rejection, we investigated their influence on the outcome of allografts in nonimmunosuppressed mouse recipie...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000155179.61445.78

    authors: Benghiat FS,Graca L,Braun MY,Detienne S,Moore F,Buonocore S,Flamand V,Waldmann H,Goldman M,Le Moine A

    更新日期:2005-03-27 00:00:00

  • Initial liver involvement in acute GVHD is predictive for nonrelapse mortality.

    abstract:BACKGROUND:Current grading systems in acute graft-versus-host disease (GVHD) are not able to identify patients with poor prognosis at time of onset, because they take into account maximal grade. The aim of this study was to evaluate potential predictors for overall survival at disease onset. METHODS:The study sample w...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3181bc2583

    authors: Robin M,Porcher R,de Castro R,Fisher G,de Latour RP,Ribaud P,Petropoulou AD,Rocha V,Devergie A,Mary JY,Socié G

    更新日期:2009-11-15 00:00:00

  • Donor-specific T-cell reactivity identifies kidney transplant patients in whom immunosuppressive therapy can be safely reduced.

    abstract:BACKGROUND:To reduce the side effects of long-term immunosuppressive therapy, stable renal transplant patients were routinely converted from cyclosporine to either azathioprine or mycophenolate mofetil. Thereafter, the azathioprine and mycophenolate mofetil dose was reduced to 75% at 4 months and to 50% at 8 months aft...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:

    authors: van Besouw NM,van der Mast BJ,de Kuiper P,Smak Gregoor PJ,Vaessen LM,IJzermans JN,van Gelder T,Weimar W

    更新日期:2000-07-15 00:00:00

  • Low pretransplant bone-mineral density and rapid bone loss do not increase risk for avascular osteonecrosis after allogeneic hematopoietic stem cell transplantation.

    abstract:BACKGROUND:Avascular osteonecrosis (AVN) is a serious complication of allogeneic stem cell transplantation (SCT). Graft-versus-host disease and its treatment with steroids are the main risk factors; underlying diagnosis, age and gender are further risk factors. It has been speculated that low baseline bone mineral dens...

    journal_title:Transplantation

    pub_type: 临床试验,杂志文章

    doi:10.1097/01.tp.0000164353.86447.db

    authors: Schulte CM,Beelen DW

    更新日期:2005-06-27 00:00:00

  • Suppressor cells in transplantation tolerance. I. Suppressor cells in the mechanism of tolerance in radiation chimeras.

    abstract::Histoincompatible-complete radiation chimeras, after resolving acute graft-versus-host disease (GVHD), establish specific tolerance to host and donor alloantigens. This tolerance can be perturbed with immunosuppressive agents and infusions of small numbers of donor-type cells, with infusions of massive numbers of dono...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198109000-00005

    authors: Tutschka PJ,Hess AD,Beschorner WE,Santos GW

    更新日期:1981-09-01 00:00:00

  • Respiratory tract viral infections in bone marrow transplant patients.

    abstract:BACKGROUND:Community respiratory viruses such as respiratory syncytial virus (RSV), adenovirus, influenza A, influenza B, and the parainfluenza group are frequent causes of respiratory disease in bone marrow transplant (BMT) patients. MATERIAL AND METHODS:During the period from March 1993 to August 1999, 810 samples o...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.TP.0000072012.26176.58

    authors: Raboni SM,Nogueira MB,Tsuchiya LR,Takahashi GA,Pereira LA,Pasquini R,Siqueira MM

    更新日期:2003-07-15 00:00:00

  • Specific tolerance and immunocompetence in haploidentical, but not in completely allogeneic, canine chimeras treated with methotrexate and cyclosporine.

    abstract::Recipient dogs were conditioned with 9.2 Gy of total-body irradiation followed by the infusion of bone marrow and peripheral blood leukocytes from a DLA-haploidentical littermate (N = 10) or a completely allogeneic unrelated donor (n = 9). Graft-vs.-host disease (GVHD) prophylaxis consisted of methotrexate (MTX) and c...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198711000-00006

    authors: Deeg HJ,Severns E,Raff RF,Sale GE,Storb R

    更新日期:1987-11-01 00:00:00

  • Exercise training in solid organ transplant recipients: a systematic review and meta-analysis.

    abstract:BACKGROUND:Exercise training is effective in improving the cardiovascular risk profiles of nontransplanted patients, but the health benefits and potential harms of routine exercise training after solid organ transplantation are unclear. This study aims to assess the health benefits and harms of supervised exercise trai...

    journal_title:Transplantation

    pub_type: 杂志文章,meta分析,评审

    doi:10.1097/TP.0b013e31827a3d3e

    authors: Didsbury M,McGee RG,Tong A,Craig JC,Chapman JR,Chadban S,Wong G

    更新日期:2013-03-15 00:00:00

  • Centrilobular necrosis in children after combined liver and small bowel transplantation.

    abstract:BACKGROUND:Centrilobular necrosis is not an uncommon finding after isolated liver transplantation. In this study, we sought to describe hepatic centrilobular necrosis in children after combined liver and small bowel transplantation (LSBT), and to assess the predictive factors, possible causes, and prognosis. METHODS:S...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200201270-00018

    authors: Lacaille F,Canioni D,Fournet JC,Revillon Y,Cezard JP,Goulet O

    更新日期:2002-01-27 00:00:00

  • Failure of cyclosporine-treated renal allograft recipients to increase glomerular filtration rate following an amino acid infusion.

    abstract::Renal allograft recipients treated with cyclosporine (CsA) have increased renal vascular resistance that falls when CsA is stopped. With the aim of identifying whether CsA-treated patients with excellent renal function also have an alteration in renal vascular tone and to investigate which vessels are affected, we hav...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198807000-00014

    authors: Cairns HS,Raval U,Neild GH

    更新日期:1988-07-01 00:00:00

  • Comparison of clinicopathologic characteristics of urothelial carcinoma between patients after renal transplantation and on dialysis.

    abstract:BACKGROUND:Urothelial carcinoma (UC) is a common complication after renal replacement therapy (RRT) among Chinese end-stage renal disease (ESRD) patients. It is unclear whether there are any differences in the clinicopathologic characteristics of UC between renal transplantation (RT) and dialysis patients; such differe...

    journal_title:Transplantation

    pub_type: 杂志文章,收录出版

    doi:10.1097/TP.0000000000000112

    authors: Zhang B,Shen C,Han WK,Yu W

    更新日期:2014-09-15 00:00:00

  • Protection of porcine aortic endothelial cells from complement-mediated cell lysis and activation by recombinant human CD59.

    abstract::Discordant xenogeneic organ transplantation is a potential solution to the critical shortage of suitable donor organs. However, clinical application of xenotransplantation with physiologically suitable organs such as those from the pig, is currently limited by the lack of agents to prevent antibody and complement-medi...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:

    authors: Kennedy SP,Rollins SA,Burton WV,Sims PJ,Bothwell AL,Squinto SP,Zavoico GB

    更新日期:1994-05-27 00:00:00

  • The Outcomes of Portal Vein Reconstruction With Vein Graft Interposition in Pediatric Liver Transplantation for Small Children With Biliary Atresia.

    abstract:BACKGROUND:Several technical modifications in portal vein (PV) reconstruction have shown excellent outcomes in pediatric liver transplantation (LT); however, which procedure is the best for PV reconstruction of the hypoplastic PV in pediatric LT remains unclear. METHODS:One hundred sixteen pediatric patients aged 1 or...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0000000000002793

    authors: Sakamoto S,Uchida H,Kitajima T,Shimizu S,Yoshimura S,Takeda M,Hirata Y,Fukuda A,Kasahara M

    更新日期:2020-01-01 00:00:00

  • Long-term Safety of Living Kidney Donation in an Emerging Economy.

    abstract:BACKGROUND:Long-term follow-up and management of donors was undertaken in a specialist kidney transplant unit in Pakistan to identify risk and prevent adverse outcomes in living related kidney donors. METHODS:In an observation cohort study between 1985 and 2012, 3748 donors were offered free medical follow-up and trea...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0000000000001075

    authors: Rizvi SA,Zafar MN,Jawad F,Aziz T,Hussain Z,Hashmi A,Hussain M,Akhtar F,Ahmed E,Naqvi R,Naqvi SA

    更新日期:2016-06-01 00:00:00

  • Population pharmacokinetics of tacrolimus in children who receive cut-down or full liver transplants.

    abstract:BACKGROUND:The aim of this study was to investigate the population pharmacokinetics of tacrolimus in pediatric liver transplant recipients and to identify factors that may explain pharmacokinetic variability. METHODS:Data were collected retrospectively from 35 children who received oral immunosuppressant therapy with ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200109270-00013

    authors: Staatz CE,Taylor PJ,Lynch SV,Willis C,Charles BG,Tett SE

    更新日期:2001-09-27 00:00:00

  • Modeling the allocation system: principles for robust design before restructuring.

    abstract::The United Network for Organ Sharing is poised to resolve geographic disparity in liver transplantation and promote allocation based on medical urgency. At the time of writing, United Network for Organ Sharing is considering redistricting the organ procurement and transplantation network so that patient model for end-...

    journal_title:Transplantation

    pub_type: 杂志文章,评审

    doi:10.1097/TP.0000000000000656

    authors: Mehrotra S,Kilambi V,Gilroy R,Ladner DP,Klintmalm GB,Kaplan B

    更新日期:2015-02-01 00:00:00

  • Long-term outcome of simultaneous kidney-pancreas transplantation: analysis of 61 patients with more than 5 years follow-up.

    abstract:BACKGROUND:The long-term outcome of simultaneous kidney pancreas transplant recipients is not well established. METHODS:We retrospectively reviewed all patients who underwent simultaneous kidney-pancreas transplantation with bladder drainage at our center between January 1989 and December 1991. A total of 57 patients ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200002270-00015

    authors: Sudan D,Sudan R,Stratta R

    更新日期:2000-02-27 00:00:00

  • Long-term duct-occluded segmental pancreatic autografts: absence of microvascular diabetic complications.

    abstract:BACKGROUND:Current insulin therapies for control of glucose metabolism in patients with type I diabetes mellitus prevent major metabolic consequences of insulin deficiency, but none prevents or arrests long-term complications. In experimental models of canine diabetes, retinopathy, neuropathy, and nephropathy have been...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199710150-00003

    authors: Hawthorne WJ,Wilson TG,Williamson P,Stewart GJ,Allen RD,Little JM,Deane SA,Ekberg H

    更新日期:1997-10-15 00:00:00

  • Post hoc analyses: after the facts.

    abstract::Prospective clinical trials are constructed with high levels of internal validity. Sample size and power considerations usually address primary endpoints. Primary endpoints have traditionally included events that are becoming increasingly less common and thus have led to growing use of composite endpoints and noninfer...

    journal_title:Transplantation

    pub_type: 杂志文章,评审

    doi:10.1097/TP.0000000000000581

    authors: Srinivas TR,Ho B,Kang J,Kaplan B

    更新日期:2015-01-01 00:00:00

  • Long-term graft outcome with mycophenolate mofetil and azathioprine: A paired kidney analysis.

    abstract:BACKGROUND:Randomized controlled trials and U.S. Registry data have demonstrated that mycophenolate mofetil (MMF) reduces acute rejection rates and improves graft survival. We undertook the first paired kidney analysis comparing the effects of MMF and azathioprine on graft outcome in the United Kingdom. METHODS:In all...

    journal_title:Transplantation

    pub_type: 杂志文章,meta分析

    doi:10.1097/01.tp.0000250713.65004.35

    authors: Shah S,Collett D,Johnson R,Thuraisingham RC,Raftery MJ,Rudge CJ,Yaqoob MM

    更新日期:2006-12-27 00:00:00

  • Statement of the Declaration of Istanbul Custodian Group Regarding Payments to Families of Deceased Organ Donors.

    abstract::Governmental and private programs that pay next of kin who give permission for the removal of their deceased relative's organs for transplantation exist in a number of countries. Such payments, which may be given to the relatives or paid directly for funeral expenses or hospital bills unrelated to being a donor, aim t...

    journal_title:Transplantation

    pub_type: 指南,杂志文章

    doi:10.1097/TP.0000000000001198

    authors: Capron AM,Delmonico FL,Dominguez-Gil B,Martin DE,Danovitch GM,Chapman J

    更新日期:2016-09-01 00:00:00

  • Precursor frequency and lytic specificity of interleukin 2 and interleukin 4-responsive murine lymphocytes.

    abstract::The response to IL-2 and IL-4 of several functionally defined lymphoid cell types was assessed at the individual responding cell level in limiting dilution experiments in which exogenous IL-2 or IL-4 was used to promote cellular activation and expansion. Splenic precursors that give rise to alloreactive CTL in LDA sup...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199004000-00018

    authors: Widmer MB,Voice RF,Conlon PJ,Grabstein KH

    更新日期:1990-04-01 00:00:00

  • Portal venous pressure changes after sequential clinical islet transplantation.

    abstract:BACKGROUND:Sequential pancreatic islet transplantation via the portal vein has led to insulin independence in patients with type 1 diabetes. Complications associated with the injection of islets into the portal vein have been reported; therefore, in this study we sought to further characterize changes in portal venous ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200210150-00002

    authors: Casey JJ,Lakey JR,Ryan EA,Paty BW,Owen R,O'Kelly K,Nanji S,Rajotte RV,Korbutt GS,Bigam D,Kneteman NN,Shapiro AM

    更新日期:2002-10-15 00:00:00

  • Characteristics of dendritic cells in rat liver.

    abstract::The properties of rat liver dendritic cells (DC) were analyzed after collagenase digestion of the tissue and enrichment with density gradient centrifugation. The liver macrophages (Kupffer cells) were eliminated by adherence before the gradient centrifugation. The morphology of isolated DC in May-Grunwald-Giemsa (MGG)...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198805000-00019

    authors: Lautenschlager I,Halttunen J,Häyry P

    更新日期:1988-05-01 00:00:00

  • Anemia after kidney transplantation; its prevalence, risk factors, and independent association with graft and patient survival: a time-varying analysis.

    abstract:INTRODUCTION:Posttransplant anemia and its association with transplant outcomes have not been properly studied. METHODS:We examined 530 renal allograft recipients transplanted at our center and followed up for 31.0±14.1 months. Hemoglobin (Hb), serum bicarbonate, and creatinine; use of erythropoiesis-stimulating agent...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e31824b36fa

    authors: Jones H,Talwar M,Nogueira JM,Ugarte R,Cangro C,Rasheed H,Klassen DK,Weir MR,Haririan A

    更新日期:2012-05-15 00:00:00

  • A suppressive effect of cyclosporine A on replication and noncoding control region activation of polyomavirus BK virus.

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    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3181c9b51c

    authors: Li YJ,Weng CH,Lai WC,Wu HH,Chen YC,Hung CC,Yang CW,Tian YC

    更新日期:2010-02-15 00:00:00

  • A randomized multicenter trial of the anti-ICAM-1 monoclonal antibody (enlimomab) for the prevention of acute rejection and delayed onset of graft function in cadaveric renal transplantation: a report of the European Anti-ICAM-1 Renal Transplant Study Gro

    abstract:BACKGROUND:T-cell activation through T-cell receptor engagement requires co-stimulatory molecules and also adhesion molecules such as ICAM-1. Moreover ICAM-1 mediates leukocyte invasion from the blood into tissue during inflammatory processes. In animal studies using mouse monoclonal antibodies against ICAM-1 (enlimoma...

    journal_title:Transplantation

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1097/00007890-199903150-00015

    authors: Salmela K,Wramner L,Ekberg H,Hauser I,Bentdal O,Lins LE,Isoniemi H,Bäckman L,Persson N,Neumayer HH,Jørgensen PF,Spieker C,Hendry B,Nicholls A,Kirste G,Hasche G

    更新日期:1999-03-15 00:00:00

  • Pharmacokinetics of the conventional and microemulsion formulations of cyclosporine in pancreas-kidney transplant recipients with gastroparesis.

    abstract::Cyclosporine (CsA) is an immunosuppressive drug requiring dose individualization and regular control due to its highly variable pharmacokinetics. Since gastroparesis may influence the absorption of CsA, a randomized cross-over study was performed to assess the pharmacokinetics and tolerability of a novel microemulsion...

    journal_title:Transplantation

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1097/00007890-199608270-00006

    authors: van der Pijl JW,Srivastava N,Denouël J,Burggraaf J,Schoemaker RC,van der Woude FJ,Cohen AF

    更新日期:1996-08-27 00:00:00