Abstract:
UNLABELLED:Tissue cells are known to respond to the stiffness of the polymer substrate on which they are grown. It has been suggested that material stiffness influences the composition of the protein layer that adsorbs to the material surface, which affects subsequent cell behavior. Previously, the stiffness of a biomaterial elastomer formed from an acrylated star-poly(d,l lactide-co-ε-caprolactone) was found to influence both fibroblast proliferation as well as the adsorption of certain proteins. However, it remained unresolved as to whether material stiffness influenced protein adsorption from serum supplemented environments and which protein(s) may have been responsible for the difference in fibroblast proliferation. Using quantitative proteomics, we show that polymer stiffness influenced the composition of the protein layers that adsorb from serum supplemented media. Fetuin A was identified as a protein that influenced fibroblast proliferation and, when combined with basic fibroblast growth factor as a medium supplement, improved fibroblast proliferation over 14days. This study is the first to correlate cell proliferation to surface adsorbed fetuin A and presents the potential new application for fetuin A as biomaterial coating or surface modifier. This work also demonstrates a novel application of quantitative proteomics for the investigation of competitive protein adsorption to biomaterial surfaces. STATEMENT OF SIGNIFICANCE:Cells are able to respond to the stiffness of their material substrate, but the method by which they sense material stiffness is still under investigation. Previously, material stiffness was found to impact the individual adsorption of fibronectin, a protein associated with cell attachment; however, it was unclear if stiffness was able to affect protein adsorption in environments with multiple proteins. This study shows that material stiffness affects the compositions of protein layers adsorbed from supplemented media, and suggests that cells may sense material stiffness via the adsorbed protein layer. Interestingly, fetuin A was found to be affecting cell proliferation and not fibronectin. Finally, this research demonstrates the use of relative quantitation proteomics as a potentially powerful method to improve biomaterial compatibility.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Vyner MC,Amsden BGdoi
10.1016/j.actbio.2015.11.039subject
Has Abstractpub_date
2016-02-01 00:00:00pages
89-98eissn
1742-7061issn
1878-7568pii
S1742-7061(15)30215-4journal_volume
31pub_type
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
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pub_type: 杂志文章,评审
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更新日期:2020-01-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2009.04.021
更新日期:2009-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.actbio.2012.03.009
更新日期:2012-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-07-15 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.actbio.2017.11.032
更新日期:2018-01-15 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.actbio.2015.01.001
更新日期:2015-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.actbio.2011.08.006
更新日期:2012-01-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2017.05.003
更新日期:2017-09-15 00:00:00
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pub_type: 杂志文章
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更新日期:2016-10-15 00:00:00
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更新日期:2015-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.actbio.2017.08.047
更新日期:2017-10-15 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2018.03.022
更新日期:2018-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.actbio.2009.10.034
更新日期:2010-04-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2010.12.016
更新日期:2011-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2021-02-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2015.07.037
更新日期:2015-10-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2012.11.033
更新日期:2013-04-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章,评审
doi:10.1016/j.actbio.2014.03.028
更新日期:2014-08-01 00:00:00