Abstract：
:Glutathione (GSH) is important in protection of cells against electrophilic drug injury and against reactive oxygen species. Both steady-state concentrations and turnover of GSH are important determinants of susceptibility of the hepatocyte to injury. Chronic ethanol administration is known to enhance susceptibility to electrophilic drug injury. We have examined the effects of chronic ethanol feeding on GSH turnover and the hepatic activities of GSH peroxidase and enzymes of the gamma-glutamyl cycle in the rat. Turnover of GSH was measured in individual animals by measuring the decrease in specific activity of GSH in bile over time after i.v. administration of [35S]cysteine. Rats fed ethanol had significantly increased rates of GSH turnover, 0.287 +/- 0.050 hr-1 vs 0.131 +/- 0.041 hr-1 (P less than 0.001), as well as steady-state GSH levels, 6.59 +/- 1.55 vs 4.30 +/- 1.28 mumoles/g liver (P less than 0.01). The activities of gamma-glutamyltransferase (GGT) and GSH-synthesizing enzymes were correspondingly increased significantly. By contrast, GSH peroxidase activity was decreased in ethanol-fed rats, 194 +/- 20.8 vs 311 +/- 89.9 nmoles NADPH oxidized/min/mg protein (P less than 0.001). Biliary output and concentrations of GSH and GSSG were similar in both groups. The increase in turnover of GSH was not due to an increase in oxidation of GSH. There was, however, an association between GSH turnover and the activity of hepatic GGT in ethanol-fed but not in control rats.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Morton S,Mitchell MCdoi
10.1016/0006-2952(85)90699-9subject
Has Abstractpub_date
1985-05-01 00:00:00pages
1559-63issue
9eissn
0006-2952issn
1873-2968pii
0006-2952(85)90699-9journal_volume
34pub_type
杂志文章abstract：:5-Fluorouracil (FUra) has been administered to mice bearing xenografts of human colon adenocarcinomas. In two tumor lines, HxGC3 and HxVRC5, intrinsically resistant to FUra, 2'-deoxyuridylate (dUMP) accumulated 13.4- and 23.9-fold above basal levels. In HxELC2 xenografts, which demonstrated some sensitivity to FUra, t...
journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(86)90281-9
更新日期：1986-04-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(89)90016-6
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(94)90467-7
更新日期：1994-11-01 00:00:00
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journal_title：Biochemical pharmacology
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更新日期：2014-09-15 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2017.10.005
更新日期：2017-12-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(94)90118-x
更新日期：1994-07-19 00:00:00
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pub_type： 杂志文章
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更新日期：2018-04-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章,评审
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更新日期：1999-02-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(90)90093-z
更新日期：1990-12-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：2010-03-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(90)90489-8
更新日期：1990-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：1983-03-15 00:00:00
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doi：10.1016/j.bcp.2016.02.015
更新日期：2016-05-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2018.04.012
更新日期：2018-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0006-2952(02)01615-5
更新日期：2003-03-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1997-08-15 00:00:00
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journal_title：Biochemical pharmacology
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更新日期：1983-03-15 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.bcp.2007.12.012
更新日期：2008-04-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：2010-09-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(87)90222-x
更新日期：1987-09-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/s0006-2952(00)00550-5
更新日期：2001-02-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/s0006-2952(96)00517-5
更新日期：1996-12-13 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(88)90414-5
更新日期：1988-10-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2009.09.013
更新日期：2010-02-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2013.06.013
更新日期：2013-09-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(88)90175-x
更新日期：1988-03-01 00:00:00
abstract：:We have demonstrated earlier that a secreted fibroblast growth factor-binding protein (FGF-BP) can enhance angiogenesis and promote tumor growth in vivo. Furthermore, we found that FGF-BP expression in squamous cell carcinoma (SCC) is reduced by concentrations of retinoids that are effective in the treatment of SCC an...
journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/s0006-2952(00)00507-4
更新日期：2000-12-01 00:00:00
