Identification of the Replication Origins from Cyanothece ATCC 51142 and Their Interactions with the DnaA Protein: From In Silico to In Vitro Studies.

Abstract:

:Based on the complete genome of Cyanothece ATCC 51142, the oriCs of both the circular and linear chromosomes in Cyanothece ATCC 51142 have been predicted by utilizing a web-based system Ori-Finder. Here, we provide experimental support for the results of Ori-Finder to identify the replication origins of Cyanothece ATCC 51142 and their interactions with the initiator protein, DnaA. The two replication origins are composed of three characteristically arranged DnaA boxes and an AT-rich stretch, and the oriC in the circular chromosome is followed by the dnaN gene. The dnaA gene is located downstream of the origin of the circular chromosome and it expresses a typical DnaA protein that is divided into four domains (I, II, III, IV), as with other members of the DnaA protein family. We purify DnaA (IV) and characterize the interaction of the purified protein with the replication origins, so as to offer experimental support for the prediction. The results of the electrophoretic mobility shift assay and DNase I footprint assay demonstrate that the C-terminal domain of the DnaA protein from Cyanothece ATCC 51142 specifically binds the oriCs of both the circular and linear chromosomes, and the DNase I footprint assay demonstrates that DnaA (IV) exhibits hypersensitive affinity with DnaA boxes in both oriCs.

journal_name

Front Microbiol

authors

Huang H,Song CC,Yang ZL,Dong Y,Hu YZ,Gao F

doi

10.3389/fmicb.2015.01370

subject

Has Abstract

pub_date

2015-12-10 00:00:00

pages

1370

issn

1664-302X

journal_volume

6

pub_type

杂志文章
  • Peeling the Layers Away: The Genomic Characterization of Bacillus pumilus 64-1, an Isolate With Antimicrobial Activity From the Marine Sponge Plakina cyanorosea (Porifera, Homoscleromorpha).

    abstract::Bacillus pumilus 64-1, a bacterial strain isolated from the marine sponge Plakina cyanorosea, which exhibits antimicrobial activity against both pathogenic and drug-resistant Gram-positive and Gram-negative bacteria. This study aimed to conduct an in-depth genomic analysis of this bioactive sponge-derived strain. The ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.592735

    authors: Freitas-Silva J,de Oliveira BFR,Vigoder FM,Muricy G,Dobson ADW,Laport MS

    更新日期:2021-01-08 00:00:00

  • A phylogenomic and molecular signature based approach for characterization of the phylum Spirochaetes and its major clades: proposal for a taxonomic revision of the phylum.

    abstract::The Spirochaetes species cause many important diseases including syphilis and Lyme disease. Except for their containing a distinctive endoflagella, no other molecular or biochemical characteristics are presently known that are specific for either all Spirochaetes or its different families. We report detailed comparati...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00217

    authors: Gupta RS,Mahmood S,Adeolu M

    更新日期:2013-07-30 00:00:00

  • Enhanced Potency of a Broad H7N9-Neutralizing Antibody HNIgGA6 Through Structure-Based Design.

    abstract::H7N9 influenza virus was first isolated in 2013 and has caused five epidemic waves among humans to date. Treatment opinions are currently limited. Previously, we characterized a human neutralizing antibody, HNIgGA6, by isolating rearranged heavy- and light-chain genes from convalescent patients. The mAb disrupts viral...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01313

    authors: Chen C,Liu Z,Liu L,Wang J,Jin Q

    更新日期:2020-06-19 00:00:00

  • Chloroplast symbiosis in a marine ciliate: ecophysiology and the risks and rewards of hosting foreign organelles.

    abstract::Simultaneous use of both heterotrophic and autotrophic metabolism ("mixotrophy") is common among protists. Strombidium rassoulzadegani is a planktonic mixotrophic marine ciliate that saves chloroplasts from its algal food and obtains a nutritional subsidy via photosynthesis. Cultures from the northeast, northwest, and...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00321

    authors: McManus GB,Schoener DM,Haberlandt K

    更新日期:2012-09-04 00:00:00

  • Expression, Purification and Characterization of the Hepatitis E Virus Like-Particles in the Pichia pastoris.

    abstract::Hepatitis E virus (HEV) is associated with acute hepatitis disease, which may lead to chronic disease in immunocompromised individuals. The disease is particularly severe among pregnant women (20-30% mortality). The only licensed vaccine against HEV, which is available in China, is the Escherichia coli purified recomb...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00141

    authors: Gupta J,Kaul S,Srivastava A,Kaushik N,Ghosh S,Sharma C,Batra G,Banerjee M,Shalimar,Nayak B,Ranjith-Kumar CT,Surjit M

    更新日期:2020-02-06 00:00:00

  • Comparison of Bacterial and Fungal Composition and Their Chemical Interaction in Free Tropospheric Air and Snow Over an Entire Winter Season at Mount Sonnblick, Austria.

    abstract::We investigated the interactions of air and snow over one entire winter accumulation period as well as the importance of chemical markers in a pristine free-tropospheric environment to explain variation in a microbiological dataset. To overcome the limitations of short term bioaerosol sampling, we sampled the atmosphe...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00980

    authors: Els N,Greilinger M,Reisecker M,Tignat-Perrier R,Baumann-Stanzer K,Kasper-Giebl A,Sattler B,Larose C

    更新日期:2020-05-20 00:00:00

  • Saccharomyces cerevisiae - Insects Association: Impacts, Biogeography, and Extent.

    abstract::Over the last few years, an increasing number of studies have reported the existence of an association between the budding yeast Saccharomyces cerevisiae and insects. The discovery of this relationship has called into question the hypothesis that S. cerevisiae is unable to survive in nature and that the presence of S....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01629

    authors: Meriggi N,Di Paola M,Cavalieri D,Stefanini I

    更新日期:2020-07-14 00:00:00

  • Low Dose Colonization of Broiler Chickens With ESBL-/AmpC- Producing Escherichia coli in a Seeder-Bird Model Independent of Antimicrobial Selection Pressure.

    abstract::Extended-spectrum beta-lactamase- (ESBL-) and AmpC beta-lactamase- (AmpC-) producing Enterobacteriaceae pose a risk for both human and animal health. For livestock, highest prevalences have been reported in broiler chickens, which are therefore considered as a reservoir of multidrug-resistant bacteria. The possibility...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02124

    authors: Robé C,Blasse A,Merle R,Friese A,Roesler U,Guenther S

    更新日期:2019-09-13 00:00:00

  • Exploration of Anti-infectives From Mangrove-Derived Micromonospora sp. RMA46 to Combat Vibrio cholerae Pathogenesis.

    abstract::Vibrio cholerae, the etiological agent of cholera, employs quorum sensing (QS) pathways to control the expression of virulence factors, including the production of cholera toxin and biofilm formation. Acquired antibiotic resistance in V. cholerae draws attention to the development of novel therapeutics that counteract...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01393

    authors: Sarveswari HB,Kalimuthu S,Shanmugam K,Neelakantan P,Solomon AP

    更新日期:2020-07-10 00:00:00

  • De novo Transcriptome Analysis of Rhizoctonia solani AG1 IA Strain Early Invasion in Zoysia japonica Root.

    abstract::Zoysia japonica brown spot was caused by necrotrophic fungus Rhizoctonia solani invasion, which led to severe financial loss in city lawn and golf ground maintenance. However, little was known about the molecular mechanism of R. solani pathogenicity in Z. japonica. In this study we examined early stage interaction bet...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00708

    authors: Zhu C,Ai L,Wang L,Yin P,Liu C,Li S,Zeng H

    更新日期:2016-05-18 00:00:00

  • The Road to Infection: Host-Microbe Interactions Defining the Pathogenicity of Streptococcus bovis/Streptococcus equinus Complex Members.

    abstract::The Streptococcus bovis/Streptococcus equinus complex (SBSEC) comprises several species inhabiting the animal and human gastrointestinal tract (GIT). They match the pathobiont description, are potential zoonotic agents and technological organisms in fermented foods. SBSEC members are associated with multiple diseases ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.00603

    authors: Jans C,Boleij A

    更新日期:2018-04-10 00:00:00

  • Microbial Community Structure and the Persistence of Cyanobacterial Populations in Salt Crusts of the Hyperarid Atacama Desert from Genome-Resolved Metagenomics.

    abstract::Although once thought to be devoid of biology, recent studies have identified salt deposits as oases for life in the hyperarid Atacama Desert. To examine spatial patterns of microbial species and key nutrient sources, we genomically characterized 26 salt crusts from three sites along a fog gradient. The communities ar...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01435

    authors: Finstad KM,Probst AJ,Thomas BC,Andersen GL,Demergasso C,Echeverría A,Amundson RG,Banfield JF

    更新日期:2017-07-28 00:00:00

  • Cell Wall-Associated Virulence Factors Contribute to Increased Resilience of Old Cryptococcus neoformans Cells.

    abstract::As Cryptococcus neoformans mother cells generationally age, their cell walls become thicker and cell-wall associated virulence factors are upregulated. Antiphagocytic protein 1 (App1), and laccase enzymes (Lac1 and Lac2) are virulence factors known to contribute to virulence of C. neoformans during infection through i...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02513

    authors: Orner EP,Bhattacharya S,Kalenja K,Hayden D,Del Poeta M,Fries BC

    更新日期:2019-11-07 00:00:00

  • Antibiotic Resistance Patterns of Pseudomonas spp. Isolated from the River Danube.

    abstract::Spread and persistence of antibiotic resistance pose a severe threat to human health, yet there is still lack of knowledge about reservoirs of antibiotic resistant bacteria in the environment. We took the opportunity of the Joint Danube Survey 3 (JDS3), the world's biggest river research expedition of its kind in 2013...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00586

    authors: Kittinger C,Lipp M,Baumert R,Folli B,Koraimann G,Toplitsch D,Liebmann A,Grisold AJ,Farnleitner AH,Kirschner A,Zarfel G

    更新日期:2016-05-03 00:00:00

  • Selection of Competitive and Efficient Rhizobia Strains for White Clover.

    abstract::The practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In conseq...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00768

    authors: Irisarri P,Cardozo G,Tartaglia C,Reyno R,Gutiérrez P,Lattanzi FA,Rebuffo M,Monza J

    更新日期:2019-04-23 00:00:00

  • Vesicles From Vibrio cholerae Contain AT-Rich DNA and Shorter mRNAs That Do Not Correlate With Their Protein Products.

    abstract::Extracellular vesicles secreted by Gram-negative bacteria have proven to be important in bacterial defense, communication and host-pathogen relationships. They resemble smaller versions of the bacterial mother cell, with similar contents of proteins, LPS, DNA, and RNA. Vesicles can elicit a protective immune response ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02708

    authors: Langlete P,Krabberød AK,Winther-Larsen HC

    更新日期:2019-11-22 00:00:00

  • Embelin Restores Carbapenem Efficacy against NDM-1-Positive Pathogens.

    abstract::The emergence and spread of carbapenemase in Gram-negative pathogens poses an enormous threat to global public health. New Delhi metallo-β-lactamase-1 (NDM-1) inactivates nearly every class of β-lactam antibiotics, including carbapenem; however, there is no clinically useful NDM-1 inhibitor. Embelin, an important ingr...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00071

    authors: Ning NZ,Liu X,Chen F,Zhou P,Hu L,Huang J,Li Z,Huang J,Li T,Wang H

    更新日期:2018-01-25 00:00:00

  • Biodegradation of Deoxynivalenol by a Novel Microbial Consortium.

    abstract::Deoxynivalenol (DON), a common mycotoxin of type B trichothecene, is produced mainly by several Fusarium species. DON causes great losses in farming and poses severe safety risks to human and animal health. Thus, DON contamination in cereals and DON toxicity are of worldwide concern. In this study, we screened the bac...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02964

    authors: Wang Y,Wang G,Dai Y,Wang Y,Lee YW,Shi J,Xu J

    更新日期:2020-01-08 00:00:00

  • Interactive Effects of Nutrients and Bradyrhizobium japonicum on the Growth and Root Architecture of Soybean (Glycine max L.).

    abstract::Understanding the symbiotic performance of rhizobia and responses of plant root systems to mineral nutrient supply will facilitate the development of strategies to enhance effective rhizobia-legume symbioses. Interactive effect of nitrogen (N), phosphorus (P), and magnesium (Mg) on the symbiotic performance of soybean...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01000

    authors: Egamberdieva D,Jabborova D,Wirth SJ,Alam P,Alyemeni MN,Ahmad P

    更新日期:2018-05-23 00:00:00

  • Protein Kinase Ime2 Is Required for Mycelial Growth, Conidiation, Osmoregulation, and Pathogenicity in Nematode-Trapping Fungus Arthrobotrys oligospora.

    abstract::Inducer of meiosis 2 (Ime2), a protein kinase that has been identified in diverse fungal species, functions in the regulation of various cellular processes, such as ascospore formation, pseudohyphal growth, and sexual reproduction. In this study, AoIme2, an ortholog of Saccharomyces cerevisiae Ime2, was characterized ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03065

    authors: Xie M,Bai N,Yang J,Jiang K,Zhou D,Zhao Y,Li D,Niu X,Zhang KQ,Yang J

    更新日期:2020-01-14 00:00:00

  • Lipids in Aspergillus flavus-maize interaction.

    abstract::In some filamentous fungi, the pathways related to the oxidative stress and oxylipins production are involved both in the process of host-recognition and in the pathogenic phase. In fact, recent studies have shown that the production of oxylipins in filamentous fungi, yeasts and chromists is also related to the develo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00074

    authors: Scarpari M,Punelli M,Scala V,Zaccaria M,Nobili C,Ludovici M,Camera E,Fabbri AA,Reverberi M,Fanelli C

    更新日期:2014-02-27 00:00:00

  • Groundwater Isolation Governs Chemistry and Microbial Community Structure along Hydrologic Flowpaths.

    abstract::This study deals with the effects of hydrodynamic functioning of hard-rock aquifers on microbial communities. In hard-rock aquifers, the heterogeneous hydrologic circulation strongly constrains groundwater residence time, hydrochemistry, and nutrient supply. Here, residence time and a wide range of environmental facto...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01457

    authors: Ben Maamar S,Aquilina L,Quaiser A,Pauwels H,Michon-Coudouel S,Vergnaud-Ayraud V,Labasque T,Roques C,Abbott BW,Dufresne A

    更新日期:2015-12-22 00:00:00

  • Expanding the Repertoire of Carbapenem-Hydrolyzing Metallo-ß-Lactamases by Functional Metagenomic Analysis of Soil Microbiota.

    abstract::Carbapenemases are bacterial enzymes that hydrolyze carbapenems, a group of last-resort β-lactam antibiotics used for treatment of severe bacterial infections. They belong to three β-lactamase classes based amino acid sequence (A, B, and D). The aim of this study was to elucidate occurrence, diversity and functionalit...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01985

    authors: Gudeta DD,Bortolaia V,Pollini S,Docquier JD,Rossolini GM,Amos GC,Wellington EM,Guardabassi L

    更新日期:2016-12-26 00:00:00

  • Biochar Treatment Resulted in a Combined Effect on Soybean Growth Promotion and a Shift in Plant Growth Promoting Rhizobacteria.

    abstract::The application of biochar to soil is considered to have the potential for long-term soil carbon sequestration, as well as for improving plant growth and suppressing soil pathogens. In our study we evaluated the effect of biochar on the plant growth of soybeans, as well as on the community composition of root-associat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00209

    authors: Egamberdieva D,Wirth S,Behrendt U,Abd Allah EF,Berg G

    更新日期:2016-02-25 00:00:00

  • Diversity and Evolution of Myxobacterial Type IV Pilus Systems.

    abstract::Type IV pili (T4P) are surface-exposed protein fibers that play key roles in the bacterial life cycle via surface attachment/adhesion, biofilm formation, motility, and development. The order Myxococcales (myxobacteria) are members of the class Deltaproteobacteria and known for their large genome size and complex socia...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01630

    authors: Sharma G,Burrows LL,Singer M

    更新日期:2018-07-19 00:00:00

  • A bioassay for the detection of benzimidazoles reveals their presence in a range of environmental samples.

    abstract::Cobamides are a family of enzyme cofactors that include vitamin B12 (cobalamin) and are produced solely by prokaryotes. Structural variability in the lower axial ligand has been observed in cobamides produced by diverse organisms. Of the three classes of lower ligands, the benzimidazoles are uniquely found in cobamide...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00592

    authors: Crofts TS,Men Y,Alvarez-Cohen L,Taga ME

    更新日期:2014-11-13 00:00:00

  • Anaerobic carboxydotrophic bacteria in geothermal springs identified using stable isotope probing.

    abstract::Carbon monoxide (CO) is a potential energy and carbon source for thermophilic bacteria in geothermal environments. Geothermal sites ranging in temperature from 45 to 65°C were investigated for the presence and activity of anaerobic CO-oxidizing bacteria. Anaerobic CO oxidation potentials were measured at up to 48.9 μm...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00897

    authors: Brady AL,Sharp CE,Grasby SE,Dunfield PF

    更新日期:2015-09-01 00:00:00

  • Identity and mechanisms of alkane-oxidizing metalloenzymes from deep-sea hydrothermal vents.

    abstract::Six aerobic alkanotrophs (organism that can metabolize alkanes as their sole carbon source) isolated from deep-sea hydrothermal vents were characterized using the radical clock substrate norcarane to determine the metalloenzyme and reaction mechanism used to oxidize alkanes. The organisms studied were Alcanivorax sp. ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00109

    authors: Bertrand EM,Keddis R,Groves JT,Vetriani C,Austin RN

    更新日期:2013-05-10 00:00:00

  • Insight into the ERVK Integrase - Propensity for DNA Damage.

    abstract::Retroviruses create permanently integrated proviruses that exist in the host genome. Retroviral genomes encode for functionally conserved gag, pro, pol, and env regions, as well as integrase (IN), which is required for retroviral integration. IN mediates viral genome insertion through 3' end processing of the viral DN...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01941

    authors: Bray S,Turnbull M,Hebert S,Douville RN

    更新日期:2016-12-01 00:00:00

  • Is Penicillin Plus Gentamicin Synergistic Against Sessile Group B Streptococcal Isolates? An in Vivo Study With an Experimental Model of Foreign-Body Infection.

    abstract::The rate of invasive group B Streptococcus (GBS) infections is steadily increasing, particularly in older persons and in adults with diabetes and other comorbidities. This population includes persons with a foreign body (e.g., who have undergone arthroplasty). In a rat tissue cage model, we evaluated the efficacy of a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00919

    authors: Ruppen C,Mercier T,Grandgirard D,Leib SL,El Haj C,Murillo O,Decosterd L,Sendi P

    更新日期:2018-05-15 00:00:00