Visualizing glycans on single cells and tissues-Visualizing glycans on single cells and tissues.

abstract：:Developing technologies such as unnatural amino acid mutagenesis, non-natural cofactor engineering, and computational design are generating proteins with novel functions; these proteins, however, often do not reach performance targets and would benefit from further optimization. Evolutionary methods can complement the...

journal_title：Current opinion in chemical biology

authors： Brustad EM,Arnold FH

更新日期：2011-04-01 00:00:00

abstract：:Conceptual and computational advances triggered an explosion of designed protein structures in the recent years. Various protein fold geometries have been robustly designed with atomic accuracy, including protein folds unseen in nature. The same principles and tools have been extended to design multi-chain assemblies....

journal_title：Current opinion in chemical biology

authors： Ljubetič A,Gradišar H,Jerala R

更新日期：2017-10-01 00:00:00

abstract：:Efficient library design is an ongoing challenge for investigators seeking novel ligands for proteins, whether for drug discovery or chemical biology. Strategies that add neglected chemistry or exclude unproductive compounds are two dominant recent themes, as is a growing awareness of molecular complexity and its impl...

journal_title：Current opinion in chemical biology

authors： Irwin JJ

更新日期：2006-08-01 00:00:00

abstract：:The fermentation of synthesis gas (including carbon monoxide, carbon dioxide, and hydrogen) with anaerobic acetogens is an established biotechnological process that has recently been transferred to a commercial scale. The natural product spectrum of acetogens is natively restricted to acetate, ethanol, and 2,3-butaned...

journal_title：Current opinion in chemical biology

authors： Molitor B,Marcellin E,Angenent LT

更新日期：2017-12-01 00:00:00

abstract：:The asymmetric bioreduction of alkenes bearing an electron-withdrawing group using flavin-dependent enzymes from the 'old yellow enzyme' family at the expense of NAD(P)H yields the corresponding non-racemic alkanes going in hand with the creation of up to two chiral carbon centres. To avoid external cofactor recycling...

journal_title：Current opinion in chemical biology

authors： Stuermer R,Hauer B,Hall M,Faber K

更新日期：2007-04-01 00:00:00

abstract：:Sugar nucleotide-modifying enzymes of the short-chain dehydrogenase/reductase type use transient oxidation-reduction by a tightly bound nicotinamide cofactor as a common strategy of catalysis to promote a diverse set of reactions, including decarboxylation, single- or double-site epimerization, and dehydration. Althou...

journal_title：Current opinion in chemical biology

authors： Borg AJE,Beerens K,Pfeiffer M,Desmet T,Nidetzky B

更新日期：2020-11-06 00:00:00

abstract：:Complex glycans participate in many essential life processes. Studies of glycan-mediated biological events have traditionally employed structurally defined fragments of the more elaborate natural molecules. However, it is now clear that this approach may sometimes be insufficient and this realization has prompted a de...

journal_title：Current opinion in chemical biology

authors： Lowary TL

更新日期：2013-12-01 00:00:00

abstract：:A long-standing goal of molecular imaging is to visualize cellular function within the context of living animals, necessitating the development of reporter genes compatible with deeply penetrant imaging modalities such as ultrasound and magnetic resonance imaging (MRI). Until recently, no reporter genes for ultrasound...

journal_title：Current opinion in chemical biology

authors： Lu GJ,Farhadi A,Mukherjee A,Shapiro MG

更新日期：2018-08-01 00:00:00

abstract：:Nitrification is the aerobic process of the nitrogen cycle that converts ammonia to nitrate and is facilitated by ammonia-oxidizing and nitrite-oxidizing microorganisms. Ammonia-oxidizers are unique chemolithotrophs that evolved specialized networks of electron carriers to generate proton motive force using ammonia as...

journal_title：Current opinion in chemical biology

authors： Stein LY

更新日期：2019-04-01 00:00:00

abstract：:Phosphorus cycling in the biosphere has traditionally been thought to involve almost exclusively transformations of the element in its pentavalent oxidation state. Recent evidence, however, suggests that a significant fraction of environmental phosphorus may exist in a more reduced form. Most abundant of these reduced...

journal_title：Current opinion in chemical biology

authors： Chin JP,McGrath JW,Quinn JP

更新日期：2016-04-01 00:00:00

abstract：:Recent advances in membrane protein crystallography have greatly increased structural information of channels permeating metal ions. Structural bioinformatics techniques and molecular dynamics calculations are providing structural models of ion channels for which the three-dimensional structure is not known. Most of t...

journal_title：Current opinion in chemical biology

authors： Giorgetti A,Carloni P

更新日期：2003-02-01 00:00:00

abstract：:Multicomponent reactions (MCRs) provide a powerful tool towards the one-pot synthesis of diverse and complex compounds on the one hand and small and 'drug-like' heterocycles on the other hand. No other single synthesis technology enables chemists to search such large chemical spaces as provided by MCRs. MCRs that invo...

journal_title：Current opinion in chemical biology

authors： Dömling A

更新日期：2002-06-01 00:00:00

abstract：:Iron is an essential nutrient for life, and its capacity to cycle between different oxidation states is required for processes spanning oxygen transport and respiration to nucleotide synthesis and epigenetic regulation. However, this same redox ability also makes iron, if not regulated properly, a potentially dangerou...

journal_title：Current opinion in chemical biology

authors： Aron AT,Reeves AG,Chang CJ

更新日期：2018-04-01 00:00:00

abstract：:Numerous tools for Zn2+ sensing in living cells have become available in the past three years. Among them, fluorescence imaging using fluorescent sensor molecules has been the most popular approach. Some of these sensor molecules can be used to visualize Zn2+ in living cells. Some of the biological functions of Zn2+ h...

journal_title：Current opinion in chemical biology

authors： Kikuchi K,Komatsu K,Nagano T

更新日期：2004-04-01 00:00:00

abstract：:The eukaryotic ribonucleoprotein reverse transcriptase (RT) telomerase uses a template within its integral RNA subunit to extend chromosome ends by synthesis of single-stranded telomeric repeats. Telomerase is adapted to its unique cellular role by an ability to release product DNA in single-stranded form, regeneratin...

journal_title：Current opinion in chemical biology

authors： Collins K

更新日期：2011-10-01 00:00:00

abstract：:Initiating and terminating free-radical reactionis via hydrogen atom transfer (HAT) is an attractive means of avoiding substrate prefunctionalization. Small molecule catalysts and reagents, however, struggle to execute this fundamental step with useful levels of diastereoselectivity and enantioselectivity. In contrast...

journal_title：Current opinion in chemical biology

authors： Nakano Y,Biegasiewicz KF,Hyster TK

更新日期：2019-04-01 00:00:00

abstract：:Thrombin is a multifunctional serine protease that plays a primary role in the pathogenic pathway of thrombosis as a consequence of its actions on the two principal components of blood clots, fibrin and activated platelets. Deficiencies in available therapies, heparin and coumadin, have recently led to an intense effo...

journal_title：Current opinion in chemical biology

authors： Ripka WC

更新日期：1997-08-01 00:00:00

abstract：:Altered metabolite levels can drive epigenetic changes critical to development and disease. However, in many cases the specific protein-metabolite interactions that underlie this process remain enigmatic. In this review, we make the case that this fundamental missing information may be discovered by applying the tools...

journal_title：Current opinion in chemical biology

authors： Kulkarni RA,Montgomery DC,Meier JL

更新日期：2019-08-01 00:00:00

abstract：:Recent studies of moonlighting functions and catalytic promiscuity provide insights into the structural and mechanistic bases of these phenomena. Moonlighting proteins that are highlighted include gephyrin, the Neurospora crassa tyrosyl tRNA synthetase, phosphoglucose isomerase, and cytochrome c. New insights into cat...

journal_title：Current opinion in chemical biology

authors： Copley SD

更新日期：2003-04-01 00:00:00

abstract：:Synthetic biology offers a bottom-up engineering approach that intends to understand complex systems via design-build-test cycles. Embryonic development comprises complex processes that originate at the level of gene regulatory networks in a cell and emerge into collective cellular behaviors with multicellular forms a...

journal_title：Current opinion in chemical biology

authors： Ebrahimkhani MR,Ebisuya M

更新日期：2019-10-01 00:00:00

abstract：:Signal amplification in conventional enzyme-based biosensors is not high enough to achieve the ultrasensitive detection of biomolecules. In recent years, signal amplification has been improved by combining enzymatic reactions with redox cycling or employing multienzyme labels per detection probe. Electrochemical-chemi...

journal_title：Current opinion in chemical biology

authors： Yang H

更新日期：2012-08-01 00:00:00

abstract：:The application of molecular cloning has revealed the phenomenal diversity and complexity of the phosphodiesterase isoenzyme family. Thus, more than 30 human phosphodiesterases are now known; all are apparently necessary for the seemingly simple task of hydrolysing the 3'-ester bond of either cyclic adenosine monophos...

journal_title：Current opinion in chemical biology

authors： Perry MJ,Higgs GA

更新日期：1998-08-01 00:00:00

abstract：:Technological advances in the automation of parallel synthesis are following the model set by the semiconductor industry: miniaturization, increasing speed, lower costs. Recent work includes preparation of high-density reaction blocks, development of ink-jet dispensing to polypropylene sheets and synthesis inside cust...

journal_title：Current opinion in chemical biology

authors： Cargill JF,Lebl M

更新日期：1997-06-01 00:00:00

abstract：:The past decade has seen rapid growth in the use of diverse compound libraries in classical phenotypic screens to identify modulators of a given process. The subsequent process of identifying the molecular targets of active hits, also called 'target deconvolution', is an essential step for understanding compound mecha...

journal_title：Current opinion in chemical biology

authors： Lee J,Bogyo M

更新日期：2013-02-01 00:00:00

abstract：:Numerous developments in useful linkers are closely related with the advances in solid-phase organic synthesis. These linkers allow facile attachment functionalization and release molecules of interest. Designing a new anchoring group can be essential for synthesis success, especially for small molecules on solid supp...

journal_title：Current opinion in chemical biology

authors： Gil C,Bräse S

更新日期：2004-06-01 00:00:00

abstract：:Histone proteins and their accompanying post-translational modifications have received much attention for their ability to affect chromatin structure and, hence, regulate gene expression. Recently, mass spectrometry has become an important complementary tool for the analysis of histone variants and modification sites,...

journal_title：Current opinion in chemical biology

authors： Garcia BA,Shabanowitz J,Hunt DF

更新日期：2007-02-01 00:00:00

abstract：:The nature of the oxidized halogen intermediate in vanadium bromoperoxidase has recently been shown to depend on the nature of the organic substrate. For example, in the presence of indoles, vanadium bromoperoxidase does not release a freely diffusible oxidized halogen intermediate (such as HOBr+/-BR2+/-Br3-). Regiose...

journal_title：Current opinion in chemical biology

authors： Butler A

更新日期：1998-04-01 00:00:00

abstract：:Electron bifurcation, or the coupling of exergonic and endergonic oxidation-reduction reactions, was discovered by Peter Mitchell and provides an elegant mechanism to rationalize and understand the logic that underpins the Q cycle of the respiratory chain. Thought to be a unique reaction of respiratory complex III for...

journal_title：Current opinion in chemical biology

authors： Peters JW,Beratan DN,Bothner B,Dyer RB,Harwood CS,Heiden ZM,Hille R,Jones AK,King PW,Lu Y,Lubner CE,Minteer SD,Mulder DW,Raugei S,Schut GJ,Seefeldt LC,Tokmina-Lukaszewska M,Zadvornyy OA,Zhang P,Adams MW

更新日期：2018-12-01 00:00:00

abstract：:Recent advances in -omic profiling technologies have ushered in an era where we no longer want to merely measure the presence or absence of a biomolecule of interest, but instead hope to understand its function and interactions within larger signaling networks. Here, we review several emerging proteomic technologies c...

journal_title：Current opinion in chemical biology

authors： Huang JX,Coukos JS,Moellering RE

更新日期：2020-02-01 00:00:00

abstract：:The atomic force microscope (AFM) allows biomolecules to be observed and manipulated under native conditions. It operates in buffer solution, produces molecular images with outstanding signal-to-noise ratio, and addresses single molecules. Progress in sample preparation and instrumentation has led to topographs that r...

journal_title：Current opinion in chemical biology

authors： Frederix PL,Akiyama T,Staufer U,Gerber Ch,Fotiadis D,Müller DJ,Engel A

更新日期：2003-10-01 00:00:00