Abstract：
:Several cell disruption methods were tested on Nannochloropsis gaditana, to evaluate their efficiency in terms of cell disintegration, energy input and release of soluble proteins. High-pressure homogenization (HPH) and bead milling were the most efficient with >95% cell disintegration, ±50% (w/w) release of total proteins and low energy input (<0.5kWh.kg-1biomass). Enzymatic treatment required low energy input (<0.34kWh.kg-1biomass), but it only released ±35% protein (w/w). Pulsed Electric Field (PEF) was neither energy-efficient (10.44kWh.kg-1biomass) nor successful for protein release (only 10% proteins w/w) and cell disintegration. The release of proteins after applying HPH and bead milling always required less intensive operating conditions for cell disruption. The energy cost per unit of released protein ranged from 0.15-0.25 €.kgProtein-1 in case of HPH, and up to 2-20 €.kgProtein-1 in case of PEF.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Safi C,Cabas Rodriguez L,Mulder WJ,Engelen-Smit N,Spekking W,van den Broek LAM,Olivieri G,Sijtsma Ldoi
10.1016/j.biortech.2017.05.012subject
Has Abstractpub_date
2017-09-01 00:00:00pages
204-210eissn
0960-8524issn
1873-2976pii
S0960-8524(17)30651-Xjournal_volume
239pub_type
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