Abstract:
:In bioartificial pancreases (BP), the number of islets needed to restore normoglycaemia in the diabetic patient is critical. However, the confinement of a high quantity of islets in a limited space may impact islet survival, particularly in regard to the low oxygen partial pressure (PO2) in such environments. The aim of the present study was to evaluate the impact of islet number in a confined space under hypoxia on cell survival. Rat islets were seeded at three different concentrations (150, 300, and 600 Islet Equivalents (IEQ)/cm(2)) and cultured in normal atmospheric pressure (160 mmHg) as well as hypoxic conditions (15 mmHg) for 24 hours. Cell viability, function, hypoxia-induced changes in gene expression, and cytokine secretion were then assessed. Notably, hypoxia appeared to induce a decrease in viability and increasing islet density exacerbated the observed increase in cellular apoptosis as well as the loss of function. These changes were also associated with an increase in inflammatory gene transcription. Taken together, these data indicate that when a high number of islets are confined to a small space under hypoxia, cell viability and function are significantly impacted. Thus, in order to improve islet survival in this environment during transplantation, oxygenation is of critical importance.
journal_name
J Diabetes Resjournal_title
Journal of diabetes researchauthors
Rodriguez-Brotons A,Bietiger W,Peronet C,Magisson J,Sookhareea C,Langlois A,Mura C,Jeandidier N,Pinget M,Sigrist S,Maillard Edoi
10.1155/2016/3615286subject
Has Abstractpub_date
2016-01-01 00:00:00pages
3615286eissn
2314-6745issn
2314-6753journal_volume
2016pub_type
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journal_title:Journal of diabetes research
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更新日期:2020-12-15 00:00:00
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journal_title:Journal of diabetes research
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journal_title:Journal of diabetes research
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更新日期:2015-01-01 00:00:00
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journal_title:Journal of diabetes research
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更新日期:2020-09-28 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Journal of diabetes research
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更新日期:2017-01-01 00:00:00
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更新日期:2017-01-01 00:00:00
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doi:10.1155/2016/3159103
更新日期:2016-01-01 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章,评审
doi:10.1155/2017/2562759
更新日期:2017-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2020-07-30 00:00:00
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pub_type: 杂志文章
doi:10.1155/2019/5487962
更新日期:2019-12-17 00:00:00
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pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00