Hepatic Na,K-ATPase development in the rat.

Abstract:

:The development of the enzyme Na,K-ATPase was studied in hepatic tissue from Sprague-Dawley rats at 18-20 days of gestation, 1, 3, 5, 7, 14, 21, and 30 days, and young adulthood (45 days). A developmental pattern was demonstrated for total and membrane-associated Na,K-ATPase activity. The activity in tissue homogenate, expressed per gram tissue, increased from late fetal life, 43.4 +/- 1.3 mumol Pi g liver-1 h-1, until 21 days of age, when an adult level of 187.3 +/- 19.6 mumol Pi g liver-1 h-1 was attained. A less pronounced ontogenic pattern was observed when enzyme levels were expressed as specific activity per milligram protein. The activity profile in a crude membrane preparation was similar. The potential for stimulation of enzyme activity by glucocorticoids was studied in 11-day-old animals injected with cortisone acetate (10 mg 100 g body weight-1) for 3 days. Enzyme specific activity was inducible: Specific activity was greater in cortisone-treated animals, 1.794 +/- .043 mumol Pi mg protein-1 h-1, versus controls, 1.258 +/- 0.043 mumol Pi mg protein-1 h-1 (p less than 0.01). We postulate that this developmental pattern for hepatic Na,K-ATPase activity may be a reflection of, or a contributing factor in, the ontogeny of sodium-dependent hepatic transport, such as that for bile salts.

authors

Duffy LF,Karl PI,Daum F,Fisher SE

doi

10.1097/00005176-198811000-00018

subject

Has Abstract

pub_date

1988-11-01 00:00:00

pages

894-901

issue

6

eissn

0277-2116

issn

1536-4801

journal_volume

7

pub_type

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