A Novel Recombinant Multi-Epitope Vaccine Could Induce Specific Cytotoxic T Lymphocyte Response In Vitro and In Vivo.

Abstract:

BACKGROUND:Malignant tumor is still one of the important diseases worldwide, cytotoxic CD8+ T lymphocytes (CTLs) play an important role in killing tumor cells. OBJECTIVE:To enhance the immune response of our previously identified HLA-A2-restricted CTL epitopes, we designed a multiepitope YL66. METHOD:The fusion protein GST-YL66 and DNA vaccine pcDNA3.1(+)-YL66 were used to induce CTLs from human peripheral blood mononuclear cells (PBMCs) of HLA-A*02+ healthy donors and and in HLA-A2.1/Kb transgenic(Tg) mice. and the activity of induced CTLs were tested by IFN-γ relesde ELISPOT assay and LDH cytotoxicity assay. RESULTS:GST-YL66 induced CTL could lysis tumor cells and release IFN-γ both in vitro and in vivo, and pcDNA3.1(+)-YL66 could also induce significant CTL response in vivo. CONCLUSION:The designed fusion multiepitope YL66 could be used as a vaccine against patients with tumors expressing COX-2 and/or MAGE-4.

journal_name

Protein Pept Lett

authors

Wu Y,Zhai W,Sun M,Zou Z,Zhou X,Li G,Yan Z,Qi Y,Gao Y

doi

10.2174/0929866524666170419152700

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

573-580

issue

6

eissn

0929-8665

issn

1875-5305

pii

PPL-EPUB-82918

journal_volume

24

pub_type

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