Abstract:
:Genome wide expression profiling of Sphingobium indicum B90A revealed induction of lin genes, linA and linB, involved in dechlorination of hexachlorocyclohexane (HCH), in the presence of all four isomers of HCH. Supporting proteomics data, the qPCR and promoter assay showed upregulation of linA transcription in the presence of HCH isomers. Analysis of the upstream region of the linA gene revealed the existence of the GntR binding site overlapping the -10 hexamer of the putative promoter motif. As GntR is a known transcription repressor its dissociation from the linA promoter is expected to induce lin genes in the presence of HCH isomers. Comparison of in situ and in-culture proteomics indicated expression lin genes at the dumpsite, an indication for the in situ HCH degradation.
journal_name
Rev Environ Healthjournal_title
Reviews on environmental healthauthors
Nandavaram A,Sagar AL,Madikonda AK,Siddavattam Ddoi
10.1515/reveh-2015-0042subject
Has Abstractpub_date
2016-03-01 00:00:00pages
57-61issue
1eissn
0048-7554issn
2191-0308pii
/j/reveh.ahead-of-print/reveh-2015-0042/reveh-2015journal_volume
31pub_type
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pub_type: 杂志文章,多中心研究,评审
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