Abstract：
:Galactose-1-phosphate uridylyltransferase (GALT) catalyzes the reversible conversion of glucose-1-phosphate and UDP-galactose to galactose-1-phosphate and UDP-glucose. This enzyme is also responsible for one of the biochemical steps that produce the precursors of agar and agarose. In this study, we report the molecular cloning and sequence analyses of a cDNA encoding GALT, from Gracilaria changii (B. M. Xia et I. A. Abbott) I. A. Abbott, J. Zhang et B. M. Xia, which constitutes a genus of seaweeds that supply more than 60% of the world's agar and agarose. We have subcloned this cDNA into a bacterial expression cloning vector and characterized the enzyme activities of its recombinant proteins in vitro. The GcGALT gene was shown to be up-regulated by salinity stresses. The abundance of transcripts encoding GcGALT was the highest in G. changii, followed by Gracilaria edulis and Gracilaria salicornia in a descending order, corresponding to their respective agar contents. Our findings indicated that GALT could be one of the components that determines the agar yield in Gracilaria species.
journal_name
J Phycoljournal_title
Journal of phycologyauthors
Siow RS,Teo SS,Ho WY,Shukor MY,Phang SM,Ho CLdoi
10.1111/j.1529-8817.2011.01105.xsubject
Has Abstractpub_date
2012-02-01 00:00:00pages
155-62issue
1eissn
0022-3646issn
1529-8817journal_volume
48pub_type
杂志文章abstract：:The ultrastructural changes which occurred during senescence in the stationary phase of growth of the unicellular green alga Spongiochloris typica were observed. The cell wall consists of a membrane like primary wall and an inner secondary wall which becomes progressively thickened with age of the culture. During sene...
journal_title：Journal of phycology
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journal_title：Journal of phycology
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journal_title：Journal of phycology
pub_type： 杂志文章,评审
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更新日期：2020-04-01 00:00:00
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journal_title：Journal of phycology
pub_type： 杂志文章
doi：10.1111/jpy.12222
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journal_title：Journal of phycology
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journal_title：Journal of phycology
pub_type： 杂志文章
doi：10.1111/j.1529-8817.1968.tb04719.x
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journal_title：Journal of phycology
pub_type： 杂志文章
doi：10.1111/j.1529-8817.2010.00946.x
更新日期：2011-02-01 00:00:00
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更新日期：2014-04-01 00:00:00
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更新日期：2011-06-01 00:00:00
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journal_title：Journal of phycology
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