Abstract:
:In Japan, the bloom seasons of two toxic species, namely, Alexandrium catenella (Whedon et Kof.) Balech and Alexandrium tamiyavanichii Balech, sometimes overlap with those of three nontoxic Alexandrium species, namely, Alexandrium affine (H. Inouye et Fukuyo) Balech, Alexandrium fraterculus (Balech) Balech, and Alexandrium pseudogoniaulax (Biecheler) T. Horig. ex Y. Kita et Fukuyo. In this study, a multiplex PCR assay has been developed that enables simultaneous detection of six Alexandrium species on the basis of differences in the lengths of the PCR products. The accuracy of the multiplex PCR system was assessed using 101 DNA templates of the six target Alexandrium species and 27 DNA templates of 11 nontarget species (128 DNA templates in total). All amplicons obtained from the 101 DNA templates of the target species were appropriately identified, whereas all 27 DNA templates of the nontarget species were not amplified. Species-specific identification by the multiplex PCR assay was certainly possible from single cells of the target species.
journal_name
J Phycoljournal_title
Journal of phycologyauthors
Nagai Sdoi
10.1111/j.1529-8817.2011.00976.xsubject
Has Abstractpub_date
2011-06-01 00:00:00pages
703-708issue
3eissn
0022-3646issn
1529-8817journal_volume
47pub_type
杂志文章abstract::Adenosine 5'-triphosphate (ATP) is a versatile extracellular signal along the tree of life, whereas cAMP plays a major role in vertebrates as an intracellular messenger for hormones, transmitters, tastants, and odorants. Since red algal spore coalescence may be considered analogous to the congregation process of socia...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12278
更新日期:2015-04-01 00:00:00
abstract::Alaria grandifolia is recorded from Nova Scotia for the first time and is briefly compared with A. esculenta from this area. ...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.1967.tb04620.x
更新日期:1967-03-01 00:00:00
abstract::Statistical growth rate modelling can be applied in a variety of ecological and biotechnological applications. Such models are frequently based on Monod or Droop equations and, especially for the latter, require reliable determination of model input parameters such as C:N quotas. Besides growth rate modelling, a C:N q...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12858
更新日期:2019-06-01 00:00:00
abstract::Characeae (Charophyceae, Charophyta) contains two tribes with six genera: tribe Chareae with four genera and tribe Nitelleae, which includes Tolypella and Nitella. This paper uses molecular and morphological data to elucidate the phylogeny of Tolypella species in North America. In the most comprehensive taxonomic trea...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12229
更新日期:2014-10-01 00:00:00
abstract::The presence of the benthic dinophysoid dinoflagellate Sinophysis canaliculata has been reported in the Canary Islands (eastern central Atlantic) in live field observations and on fixed macroalgal samples from intertidal ponds (26 sampling sites from El Hierro, Tenerife, Gran Canaria, Fuerteventura and Lanzarote islan...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12508
更新日期:2017-04-01 00:00:00
abstract::As part of a long-term study on the biology and ecology of the intertidal kelp Lessonia nigrescens Bory, we report on the occurrence of gall development on this alga, identified the possible causal agent, and assessed the extent of the phenomenon in two wild stands of the host. Our results showed that galls affecting ...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.2009.00749.x
更新日期:2009-12-01 00:00:00
abstract::The Conchocelis phases of Porphyra perforata f. patens, P. cuneiformis and P. nereocystis were cultured from spores in a sterile artificial medium at 12 C and with 25 ft-c illumination for 10 hr daily. The cultures showed differences in duration of the vegetative phase, sporulation, liberation of spores, and the retur...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.1969.tb02574.x
更新日期:1969-03-01 00:00:00
abstract::Cysts belonging to the benthic dinoflagellate Bysmatrum subsalsum were recovered from palynologically treated sediments collected in the Alvarado Lagoon (southwestern Gulf of Mexico). The cysts are proximate, reflecting the features of the parent thecal stage, and their autofluorescence implies a dinosporin compositio...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12257
更新日期:2015-02-01 00:00:00
abstract::Cyanobacteria occupy many niches within terrestrial, planktonic, and benthic habitats. The diversity of habitats colonized, similarity of morphology, and phenotypic plasticity all contribute to the difficulty of cyanobacterial identification. An unknown marine filamentous cyanobacterium was isolated from an aquatic an...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12490
更新日期:2017-02-01 00:00:00
abstract::Despite worldwide proliferation of the genus Caulerpa and subsequent effects on benthic communities, little is known about the nutritional physiology of the Caulerpales. Here, we investigated the uptake rates of ammonium, nitrate, amino acids, and phosphate through the fronds and rhizoids + stolon, the internal transl...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12988
更新日期:2020-06-01 00:00:00
abstract::Here, we established the cyst-motile stage relation-ship for Spiniferites pachydermus through incubation of cysts with a characteristically microreticulate/perforate surface isolated from Izmir Bay in the eastern Aegean Sea of the eastern Mediterranean. The morphology of the motile stage was similar to Gonyaulax spini...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12304
更新日期:2015-06-01 00:00:00
abstract::Microbial eukaryotes may extinguish much of their nuclear phylogenetic history due to endosymbiotic/horizontal gene transfer (E/HGT). We studied E/HGT in 32,110 contigs of expressed sequence tags (ESTs) from the dinoflagellate Alexandrium tamarense (Dinophyceae) using a conservative phylogenomic approach. The vast maj...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.2012.01194.x
更新日期:2012-10-01 00:00:00
abstract::Three species of tropical oceanic phytoplankton were isolated from two locations in the eastern Pacific Ocean. Unialgal cultures were maintained in an enriched seawater medium. The effects of temperature, and, in separate experiments, illuminance, on the exponential cell division rates of those algae were investigated...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.1966.tb04586.x
更新日期:1966-03-01 00:00:00
abstract::Studies of quantitative trait loci based on genetic linkage maps require the establishment of a mapping population. Permanent mapping populations are more ideal than temporary ones because they can be used repeatedly. However, there has been no reported permanent sporophyte population of economically important kelp sp...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.13066
更新日期:2020-09-04 00:00:00
abstract::Despite studies suggesting that most seaweeds are poor dispersers, many red algal species are reported to have circumglobal distributions. Such distributions have mostly been based on morphological identifications, but molecular data have revealed a range of issues with morphologically defined species boundaries. Cons...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12778
更新日期:2018-12-01 00:00:00
abstract::The publication of a mini-review by Olivier De Clerck et al. in this issue of the Journal of Phycology presented an opportunity to open a dialogue on challenges faced by contemporary algal taxonomists. The Editorial Office solicited the following two additional contributions in response to De Clerck et al.'s paper; th...
journal_title:Journal of phycology
pub_type: 信件
doi:10.1111/jpy.12041
更新日期:2013-04-01 00:00:00
abstract::Since the first phylogenetic study of the order Batrachospermales, Batrachospermum was shown to be paraphyletic. Subsequently, sections of the genus have been methodically investigated using DNA sequences and morphology in order to propose new genera and delineate species. Batrachospermum section Turfosa is the last s...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.13001
更新日期:2020-08-01 00:00:00
abstract::Ulva Linnaeus (Ulvophyceae, Ulvales) is a genus of green algae widespread in different aquatic environments. Members of this genus show a very simple morphology and a certain degree of phenotypic plasticity, heavily influenced by environmental conditions, making difficult the delineation of species by morphological fe...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12005
更新日期:2012-12-01 00:00:00
abstract::Proliferating cell nuclear antigen (PCNA) plays critical roles in eukaryotic DNA replication and replication-associated processes. It is typically encoded by one or two gene copies (pcna) in eukaryotic genomes. Recently reported higher copy numbers of pcna in some dinoflagellates raised a question of how this gene has...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12815
更新日期:2019-02-01 00:00:00
abstract::Antarctica is one of the most difficult habitats for sustaining life on earth; organisms that live there have developed different strategies for survival. Among these organisms is the green alga Prasiola crispa, belonging to the class Trebouxiophyceae. The literature on P. crispa taxonomy is scarce, and many gaps in t...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12541
更新日期:2017-08-01 00:00:00
abstract::Some abiotic conditions are well known to play disproportionately large roles in shaping contemporary assemblages, yet their roles may not continue to have similar magnitudes of effect into the future. We tested whether forecasted levels of CO2 could alter the strength of influence of an abiotic factor (i.e., light in...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.2011.01002.x
更新日期:2011-08-01 00:00:00
abstract::Partitioning of the carbon (C) fixed during photosynthesis between neutral lipids (NL) and carbohydrates was investigated in Isochrysis sp. (Haptophyceae) in relation to its nitrogen (N) status. Using batch and nitrate-limited continuous cultures, we studied the response of these energy reserve pools to both condition...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.2012.01154.x
更新日期:2012-06-01 00:00:00
abstract::New strains of a wall-less unicellular volvocalean flagellate were isolated from a freshwater environment in Japan. Observations of the alga, described here as Hapalochloris nozakii Nakada, gen. et sp. nov., were made using light, fluorescence, and electron microscopy. Each vegetative cell had two flagella, four contr...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12484
更新日期:2017-02-01 00:00:00
abstract::Chlorella-like coccoid green algae are widely distributed in almost all terrestrial habitats and belong to different lineages of the Chlorophyceae and Trebouxiophyceae. The Watanabea clade of the Trebouxiophyceae shows a high genetic biodiversity. Re-investigation of the authentic strain of the rarely found W. renifor...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/jpy.12830
更新日期:2019-04-01 00:00:00
abstract::Asexual reproduction by cloning may affect the genetic structure of populations, their potential to evolve, and, among foundation species, contributions to ecosystem functions. Macroalgae of the genus Fucus are known to produce attached plants only by sexual recruitment. Recently, however, clones of attached plants re...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.2011.01032.x
更新日期:2011-10-01 00:00:00
abstract::The marine filamentous nonheterocystous nitrogen-fixing cyanobacterium Lyngbya aestuarii (F. K. Mert.) Liebman ex Gomont CCY 9616 was grown under diazotrophic and nondiazotrophic conditions and under an alternating 16:8 light:dark (L:D) regime. Nitrogenase activity appeared just before the onset of the dark period, re...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.2009.00714.x
更新日期:2009-08-01 00:00:00
abstract::Light has been identified as one of the main factors affecting seaweed ecophysiology. We investigated the dependence of nutrient metabolism on sun and shade light conditions and whether episodes of upwelling of nutrient-rich subsuperficial water could reduce the summer nutrient limitation driving physiological changes...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.2007.00440.x
更新日期:2008-02-01 00:00:00
abstract::The effect of light, of 2 wavelengths, and of 3 temperatures on the uptake and evolution of O2 by the antarctic marine diatom, Fragilaria sublinearis, has been examined with mass spectrometry. The responses of this organism to light, in terms of O2 exchange, were qualitatively similar to those recorded by Hoch et al. ...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.1966.tb04601.x
更新日期:1966-09-01 00:00:00
abstract::Solar ultraviolet radiation (UVR, 280-400 nm) is known to affect macroalgal physiology negatively, while nutrient availability may affect UV-absorbing compounds (UVACs) and sensitivity to UVR. However, little is known about the interactive effects of UVR and nitrate availability on macroalgal growth and photosynthesis...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.2009.00654.x
更新日期:2009-04-01 00:00:00
abstract::To investigate the changes in the morphology and polysaccharide content of Microcystis aeruginosa (Kütz.) Kütz. during flagellate grazing, cultures of M. aeruginosa were exposed to grazing Ochromonas sp. for a period of 9 d under controlled laboratory conditions. M. aeruginosa responded actively to flagellate grazing ...
journal_title:Journal of phycology
pub_type: 杂志文章
doi:10.1111/j.1529-8817.2008.00502.x
更新日期:2008-06-01 00:00:00