Abstract:
:The direct electron detector has revolutionized electron cryo-microscopy (CryoEM). Icosahedral virus structures are routinely produced at 4Å resolution or better and the approach has largely displaced virus crystallography, as it requires less material, less purity and often produces a structure more rapidly. Largely ignored in this new era of CryoEM is the dynamic information in the data sets that was not available in X-ray structures. Here we review an approach that captures the dynamic character of viruses displayed in the CryoEM ensemble of particles at the moment of freezing. We illustrate the approach with a simple model, briefly describe the details and provide a practical application to virus particle maturation.
journal_name
Curr Opin Viroljournal_title
Current opinion in virologyauthors
Doerschuk PC,Gong Y,Xu N,Domitrovic T,Johnson JEdoi
10.1016/j.coviro.2016.02.011subject
Has Abstractpub_date
2016-06-01 00:00:00pages
57-63eissn
1879-6257issn
1879-6265pii
S1879-6257(16)30015-3journal_volume
18pub_type
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journal_title:Current opinion in virology
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journal_title:Current opinion in virology
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journal_title:Current opinion in virology
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journal_title:Current opinion in virology
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journal_title:Current opinion in virology
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journal_title:Current opinion in virology
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journal_title:Current opinion in virology
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journal_title:Current opinion in virology
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journal_title:Current opinion in virology
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journal_title:Current opinion in virology
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journal_title:Current opinion in virology
pub_type: 杂志文章,评审
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journal_title:Current opinion in virology
pub_type: 杂志文章,评审
doi:10.1016/j.coviro.2014.02.003
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journal_title:Current opinion in virology
pub_type: 杂志文章,评审
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journal_title:Current opinion in virology
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abstract::The rVSV-ZEBOV vaccine is currently the only Ebola vaccine with demonstrated clinical efficacy in a ring-vaccination clinical trial. It has been shown to be reactogenic but immunogenic and safe in several Phase I clinical studies. However, its mechanisms of protection are unknown and available immunogenicity data are ...
journal_title:Current opinion in virology
pub_type: 杂志文章,评审
doi:10.1016/j.coviro.2017.03.008
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journal_title:Current opinion in virology
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