Ischemic small intestine-in vivo versus ex vivo bioimpedance measurements.

Abstract:

OBJECTIVE:Bioimpedance has been used to investigate changes in electrical parameters during ischemia in various tissues. The small intestine is a multi-layered structure, with several distinct tissue types, and ischemia related changes occur at different times in the different intestinal layers. When investigating how the electrical properties in the small intestine is affected by ischemia, some researchers have used ex vivo models while others have used in vivo models. In this study, we compare ischemic time development of electrical parameters in ischemic in vivo versus ex vivo small intestine. APPROACH:Measurements were performed using a two-electrode setup, with a Solartron 1260/1294 impedance gain-phase analyser. Electrodes were placed on the surface of ischemic pig jejunum, applying a voltage and measuring the resulting electrical admittance. In each pig, 4 segments of the jejunum were made ischemic by clamping the mesenteric arteries and veins, resulting in a 30 cm central zone of warm ischemia and edema. The in vivo part of the experiment lasted 10 h, after which 3 pieces of perfused small intestine were resected, stored in Ringer-acetat at 38 °C, and measured during a 10 h ex vivo experiment. Main results and significance: We found significant differences (p  <  0.0001) between the values of electric parameters when comparing the in vivo and ex vivo measurements as a function of ischemic time development. We also observed some similarities in the trends. In vivo, we measured an overall decrease in impedance during the duration of the experiment, probably as a result from the formation of edema. Ex vivo, the low frequency impedance increased initially for approximately 3 h before starting to decrease.

journal_name

Physiol Meas

authors

Strand-Amundsen RJ,Reims HM,Tronstad C,Kalvøy H,Martinsen ØG,Høgetveit JO,Ruud TE,Tønnessen TI

doi

10.1088/1361-6579/aa67b7

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

715-728

issue

5

eissn

0967-3334

issn

1361-6579

journal_volume

38

pub_type

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