Abstract:
BACKGROUND:Anoikis is a specialized cell apoptosis triggered by loss of cell adhesion. Acquisition of anoikis resistance is a key cancer hallmark that has been associated with metastasis of human malignancies. Cholangiocarcinoma (CCA) is a highly metastatic cancer of the bile duct, in which mechanisms underlying its resistance to anoikis has not been well elucidated. METHODS:HuCCT1 and Huh28 were utilized as in vitro models to examine biological characteristics of anoikis resistant-CCA cells. Expression and activation of signaling molecules were determined by western blotting. RESULTS:We demonstrated that a significant proportion of CCA cells including HuCCT1 and Huh28 resisted anoikis induced by anchorage-independent condition. Notably, anoikis-resistant CCA cells, especially HuCCT1, substantially increased invasive potential and became resistant to gemcitabine, which is the standard chemotherapeutic agent for CCA. Mechanistically, the anoikis-resistant HuCCT1 cells markedly increased activation of signal transducer and activator of transcription 3 (STAT3) and concomitantly upregulated anti-apoptotic proteins, Mcl-1 and Bcl-2. Inhibition of STAT3 by AG 490 significantly induced HuCCT1 cells to undergo anoikis. CONCLUSIONS:Taken together, our findings uncover the aggressive properties of CCA cells that overcome anoikis, and reveal the involvement of STAT3 signaling pathway in anoikis resistance in CCA. Targeting STAT3 may restore anoikis and prevent metastasis of the disease.
journal_name
J Hepatobiliary Pancreat Scijournal_title
Journal of hepato-biliary-pancreatic sciencesauthors
Huyen NT,Prachayasittikul V,Chan-On Wdoi
10.1002/jhbp.354subject
Has Abstractpub_date
2016-07-01 00:00:00pages
397-405issue
7eissn
1868-6974issn
1868-6982journal_volume
23pub_type
杂志文章abstract::Intrahepatic cholangiocarcinomas (ICCs) are made up of heterogenous carcinomas arising from different anatomical sites of the liver. Two types of candidate stem/progenitor cells of the biliary tree are postulated to exist at the peribiliary glands for large bile ducts and at the canals of Hering for small ducts and he...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章,评审
doi:10.1002/jhbp.154
更新日期:2015-02-01 00:00:00
abstract::Mucobilia is a rare pathologic condition characterized by the abnormal secretion and accumulation of abundant mucus within the biliary tree. It is usually seen in association with mucin-producing hepatobiliary and pancreatic tumors. Neoplastic transformation of these tumors can range from low-grade dysplasia to invasi...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章,评审
doi:10.1007/s00534-009-0150-2
更新日期:2010-05-01 00:00:00
abstract:BACKGROUND:We investigated the aberrant expression of the STAT family in humans and liver fluke (Opisthorchis viverrini, Ov)-induced hamster cholangiocarcinoma (CCA) tissues. METHODS:The expression and phosphorylation of STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b and STAT6 in human hamster CCA tissues were immunohisto...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.131
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:There are no multicenter studies on the influence of diabetes duration on pancreatic transplant outcomes. Our study aimed to determine how type 1 diabetes duration influenced survival of pancreatic grafts. METHODS:The data of 8,139 patients who received pancreas transplants during 2006-2015 were extracted f...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.677
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND/PURPOSE:Recent introduction of multi-detector CT (MDCT) and high-speed magnetic resonance (MR) imaging have dramatically advanced visualization and imaging technology in diagnostic and therapeutic strategy in hepatobiliary pancreatic disease. However, image diagnostics have progressed with a background of th...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章,评审
doi:10.1007/s00534-009-0192-5
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND/PURPOSE:S-1 is a new oral fluoropyrimidine anticancer agent shown to be effective for pancreatic cancer. In a previous phase I trial, we evaluated the safety of S-1 combined with radiotherapy to determine the maximum tolerated dose and dose-limiting toxicity in patients with unresectable pancreatic cancer. T...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00534-011-0400-y
更新日期:2012-03-01 00:00:00
abstract::Liver function reserve estimation is important for selecting the appropriate patients for hepatectomy or ablation of tumors. Many liver function tests have been devised, but the indocyanine green (ICG) clearance test remains the most popular for its simplicity and perhaps accuracy. Compared with the Child-Pugh classif...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章,评审
doi:10.1007/s00534-009-0229-9
更新日期:2010-07-01 00:00:00
abstract::Liver transplantation (LT) has been regarded as "potentially curative" in a cirrhotic patient with hepatocellular carcinoma (HCC) because it removes the cancer and eradicates the cirrhosis. In Taiwan, HCC ranks first among the leading causes of cancer mortality in males and 4th in females. The most common causes are c...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章,评审
doi:10.1007/s00534-009-0166-7
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:The rate of preoperative biliary drainage for pancreaticoduodenectomy has been increasing despite most recent evidence that favors avoiding it. Only a few studies have focused on late surgical complications - biliary stricture after pancreaticoduodenectomy and have produced only inconclusive results. We eval...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.559
更新日期:2018-06-01 00:00:00
abstract:INTRODUCTION:A stronger evidence level is needed to confirm the benefits and limits of laparoscopic hemihepatectomies. METHODS:Laparoscopic and open hemihepatectomies from nine European referral centers were compared after propensity score matching (right and left hemihepatectomies separately, and benign and malignant...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章,多中心研究
doi:10.1002/jhbp.662
更新日期:2020-01-01 00:00:00
abstract::Segmentectomy is anatomical resection of segments based on the classification of Couinaud. This procedure is performed mainly for hepatocellular carcinoma. Invasion of portal vein and intrahepatic metastases often occur with hepatocellular carcinoma. Thus, it is desirable to perform anatomical resection of the cancer-...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1007/s00534-011-0448-8
更新日期:2012-01-01 00:00:00
abstract:AIM:The aim of this study was to evaluate the outcomes following upfront pancreaticoduodenectomy (PD) in severely jaundiced (serum bilirubin level ≥15 mg/dl) patients with malignant distal common bile duct (CBD) obstruction. BACKGROUND:Recent studies have failed to show the benefits of preoperative biliary drainage (P...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.671
更新日期:2019-11-01 00:00:00
abstract:BACKGROUND/PURPOSE:Living-donor pancreas transplants (LDPs) were introduced at Chiba-East National Hospital in 2004, and 12 LDPs have been performed at this institution to date. Based on the outcome of these 12 LDPs, the efficacy and safety of LDPs are herein discussed. METHODS:Twelve diabetic patients underwent LDPs;...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1007/s00534-009-0148-9
更新日期:2010-03-01 00:00:00
abstract:BACKGROUND/PURPOSE:To prevent or reduce hepatic complications after hepatectomy, it is important to employ preoperative predictive parameters and to determine the indications for hepatectomy. In the present study, we evaluated risk parameters in patients who underwent hepatectomy between 1994 and 2003, and selected thr...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1007/s00534-010-0281-5
更新日期:2010-11-01 00:00:00
abstract:BACKGROUND:Recently, a new digital cholangiopancreatoscopy (DCPS) has been developed. The aim of this study is to retrospectively evaluate the utility of new DCPS in patients with pancreaticobiliary diseases. METHODS:Digital cholangiopancreatoscopy was used in 26 patients (16 men) with pancreaticobiliary diseases that...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.328
更新日期:2016-04-01 00:00:00
abstract:BACKGROUND:The use of cadaveric vein allografts was first described by our group as a feasible option for venous reconstruction. The aim of this study was to report long-term results of this innovative technique. METHODS:Cold-stored veins harvested from donor cadavers were used as homologous grafts for venous reconstr...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.299
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:A total of 26 pancreas transplants from brain dead donors, including 21 simultaneous pancreas and kidney (SPK) transplantation procedures, have been performed at Fujita Health University Hospital since the new pancreas transplant program was initiated in August 2012. The objective of this study is to investi...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.339
更新日期:2016-05-01 00:00:00
abstract::Surgical resection, considered the optimal treatment of biliary papillomatosis, often remains incomplete due to high risk of recurrence in view of positive margins or recurrence on the remnant bile duct because of its multifocality. Resection of the whole biliary tree by liver transplantation and duodenopancreatectomy...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1007/s00534-009-0151-1
更新日期:2010-05-01 00:00:00
abstract:BACKGROUND/PURPOSE:To describe surgical techniques and results of right hepatic lobectomy (RHL) with thoracotomy. METHODS:This procedure consists of laparotomy and thoracotomy, cholecystectomy, division of the right hepatic artery and right portal vein, mobilization of the liver, division of the right hepatic vein, tr...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1007/s00534-011-0446-x
更新日期:2012-01-01 00:00:00
abstract:INTRODUCTION:Laparoscopic cholecystectomy (LC) is considered difficult in patients with an impacted gallstone (IG). We examined the efficacy of releasing an IG after percutaneous transhepatic gallbladder drainage (PTGBD) for acute cholecystitis (AC) and the usefulness of the Difficulty Score (DS) proposed in the Tokyo ...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.857
更新日期:2020-10-31 00:00:00
abstract:BACKGROUND:Indocyanine green (ICG) is a synthetic dye that is widely used to evaluate liver function in critically ill patients, before liver resection or after liver transplantation. Controversy still exists about the impact exerted on the ICG ratio after 15 min (ICG R15) by differences in liver perfusion rates, hyper...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1007/s00534-009-0160-0
更新日期:2010-03-01 00:00:00
abstract:BACKGROUND:Our previous randomized controlled trial revealed no difference in 2-year overall survival (OS) between extended and standard resection for pancreatic adenocarcinoma. The present study evaluated the 5-year OS and recurrence patterns according to the extent of pancreatectomy. METHODS:Between 2006 and 2009, 1...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1002/jhbp.465
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Latent pancreatic fistula (LPF) is difficult to diagnose during the early postoperative phase because of initially normal drain fluid amylase (DFA) levels. The present study investigated the clinical significance and risk factors of LPF after pancreatoduodenectomy. METHODS:A total of 662 patients who underw...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.820
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND:Surgical indications for liver hemangioma remain unclear. METHODS:Of 510 patients undergoing surgical resection for liver hemangioma in 118 Japanese centers between 1998 and 2012, abdominal symptoms, diagnostic accuracy, and surgical outcomes were analyzed to propose size-based surgical indications. Patient...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.464
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:The influence of donor-recipient gender mismatch on outcomes after liver transplantation (LT) is controversial. The aim of this study was to evaluate the effect of donor and recipient gender discordance on graft survival. METHODS:All patients who underwent primary LT from 1994-2012 at a single-center were i...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.40
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND:Intrahepatic cholangiocarcinoma (IHC) is a rare liver malignancy with a rising incidence worldwide. Since no standard treatment has been established so far, the aim of this study was to assess the safety and efficacy of repeated liver resection and/or radiofrequency ablation (RFA) in selected cases with recu...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1007/s00534-009-0256-6
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:Resection using a stapler is a popular approach to distal pancreatectomy. However, the resulting leakage of pancreatic juice represents a serious problem. We have developed a force-fluorescence measurement as a first step towards the quantitative evaluation of pancreatic leakage due to tissue tearing under c...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.775
更新日期:2020-11-01 00:00:00
abstract:BACKGROUND/PURPOSE:The purpose of the present study was to assess long-term outcomes following liver resection for colorectal liver metastases (CRLM) with concurrent extrahepatic disease and to identify the preoperative prognostic factors for selection of operative candidates. METHODS:In this retrospective, multi-inst...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1002/jhbp.810
更新日期:2020-11-01 00:00:00
abstract:BACKGROUND:Since the publication of the Tokyo Guidelines (TG13) for the management of acute cholecystitis (AC), multidirectional studies have been published. However, epidemiological research about AC with big data was not projected. The aim of this study was to reveal the actual clinical conditions of AC. METHOD:The ...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章,多中心研究
doi:10.1002/jhbp.450
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND/PURPOSE:Sinisan, a traditional Chinese medicine, is effective for the treatment of gastrointestinal disorders. In this study, we investigated the potential protective role of Sinisan against chronic pancreatitis (CP) in rats. METHODS:CP was induced in rats by intrapancreatic injection of trinitrobenzene sul...
journal_title:Journal of hepato-biliary-pancreatic sciences
pub_type: 杂志文章
doi:10.1007/s00534-010-0368-z
更新日期:2011-07-01 00:00:00