Advantages and Limitations of Direct PCR Amplification of Bacterial 16S-rDNA from Resected Heart Tissue or Swabs Followed by Direct Sequencing for Diagnosing Infective Endocarditis: A Retrospective Analysis in the Routine Clinical Setting.

Abstract:

:Infective endocarditis (IE) is a life-threatening disease that is associated with high morbidity and mortality. Its long-term prognosis strongly depends on a timely and optimized antibiotic treatment. Therefore, identification of the causative pathogen is crucial and currently based on blood cultures followed by characterization and susceptibility testing of the isolate. However, antibiotic treatment starting prior to blood sampling or IE caused by fastidious or intracellular microorganisms may cause negative culture results. Here we investigate the additional diagnostic value of broad-range PCR in combination with direct sequencing on resected heart tissue or swabs in patients with tissue or swab culture-negative IE in a routine clinical setting. Sensitivity, specificity, and positive and negative predictive values of broad-range PCR from diagnostic material in our patients were 33.3%, 76.9%, 90.9%, and 14.3%, respectively. We identified a total of 20 patients (21.5%) with tissue or culture-negative IE who profited by the additional application of broad-range PCR. We conclude that broad-range PCR on resected heart tissue or swabs is an important complementary diagnostic approach. It should be seen as an indispensable new tool for both the therapeutic and diagnostic management of culture-negative IE and we thus propose its possible inclusion in Duke's diagnostic classification scheme.

journal_name

Biomed Res Int

authors

Maneg D,Sponsel J,Müller I,Lohr B,Penders J,Madlener K,Hunfeld KP

doi

10.1155/2016/7923874

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

7923874

eissn

2314-6133

issn

2314-6141

journal_volume

2016

pub_type

杂志文章
  • In vitro culture-induced pluripotency of human spermatogonial stem cells.

    abstract::Unipotent spermatogonial stem cells (SSCs) can be transformed into ESC-like cells that exhibit pluripotency in vitro. However, except for mouse models, their characterization and their origins have remained controversies in other models including humans. This controversy has arisen primarily from the lack of the direc...

    journal_title:BioMed research international

    pub_type: 临床试验,杂志文章

    doi:10.1155/2013/143028

    authors: Lim JJ,Kim HJ,Kim KS,Hong JY,Lee DR

    更新日期:2013-01-01 00:00:00

  • Relationship between Physical Activity and Physical Fitness in Preschool Children: A Cross-Sectional Study.

    abstract:Purpose:To evaluate the relationships between objectively measured physical activity and physical fitness among preschool children. Methods:A total of 346 participants (201 boys and 145 girls) aged 3.5-5.5 years (M = 4.5 yr, SD = 0.47) from Shanghai, China, completed physical fitness assessments, including triceps ski...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2017/9314026

    authors: Fang H,Quan M,Zhou T,Sun S,Zhang J,Zhang H,Cao Z,Zhao G,Wang R,Chen P

    更新日期:2017-01-01 00:00:00

  • Tyrosine kinase inhibitors induced thyroid dysfunction: a review of its incidence, pathophysiology, clinical relevance, and treatment.

    abstract::Tyrosine kinase inhibitors (TKI) belong to a new class of molecular multitargeted anticancer therapy which targets different growth factor receptors and hence attenuates cancer cell survival and growth. Since their introduction as adjunct treatment for renal cell carcinoma and gastrointestinal stromal tumors (GIST), a...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2013/725410

    authors: Ahmadieh H,Salti I

    更新日期:2013-01-01 00:00:00

  • Peripheral leukocyte apoptosis in patients with Parkinsonism: correlation with clinical characteristics and neuroimaging findings.

    abstract::Apoptosis of both brain neurons and peripheral blood leukocyte is believed to be an important biomarker for evaluating the functional status of Parkinson's disease (PD). However, their correlation remains unknown. A better understanding of the pathophysiology of neurodegeneration is essential for the treatment and pre...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2014/635923

    authors: Lin WC,Tsai NW,Huang YC,Cheng KY,Chen HL,Li SH,Kung CT,Su YJ,Lin WM,Chen MH,Chiu TM,Yang IH,Lu CH

    更新日期:2014-01-01 00:00:00

  • The effect of nicotine dependence on psychopathology in patients with schizophrenia.

    abstract:INTRODUCTION:Our study aims to determine the prevalence of nicotine dependence and investigate the effect of nicotine dependence on psychopathology among schizophrenia patients. METHODS:A cross-sectional study was carried out in an outpatient psychiatric clinic at a general hospital in Malaysia. 180 recruited subjects...

    journal_title:BioMed research international

    pub_type: 临床试验,杂志文章

    doi:10.1155/2015/730291

    authors: Yee A,Bt Nek Mohamed NN,Binti Hashim AH,Loh HS,Harbajan Singh MK,Ng CG,Jambunathan ST

    更新日期:2015-01-01 00:00:00

  • Knockdown of Matrix Metallopeptidase 9 Inhibits Metastasis of Oral Squamous Cell Carcinoma Cells in a Zebrafish Xenograft Model.

    abstract::Destruction of extracellular matrix (ECM) is one of the basic steps of tumor invasion and metastasis. Matrix metalloproteinase (MMP) 9, a kind of zinc-ion-dependent endopeptidase, can degrade almost all protein components in the ECM, destroy the histological barrier of tumor cell invasion, and play a key role in tumor...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/4350783

    authors: Wen J,Yin P,Li L,Kang G,Ning G,Cao Y,Gao F,Su Y,Wu Y,Zhang X

    更新日期:2020-04-15 00:00:00

  • Aberrant Methylation and Differential Expression of SLC2A1, TNS4, GAPDH, ATP8A2, and CASZ1 Are Associated with the Prognosis of Lung Adenocarcinoma.

    abstract::Lung cancer is one of the leading triggers for cancer death worldwide. In this study, the relationship of the aberrantly methylated and differentially expressed genes in lung adenocarcinoma (LUAD) with cancer prognosis was investigated, and 5 feature genes were identified eventually. Specifically, we firstly downloade...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/1807089

    authors: Wang X,Shi D,Zhao D,Hu D

    更新日期:2020-09-25 00:00:00

  • Somatostatin receptor-based molecular imaging and therapy for neuroendocrine tumors.

    abstract::Neuroendocrine tumors (NETs) are tumors originated from neuroendocrine cells in the body. The localization and the detection of the extent of NETs are important for diagnosis and treatment, which should be individualized according to the tumor type, burden, and symptoms. Molecular imaging of NETs with high sensitivity...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2013/102819

    authors: Wang L,Tang K,Zhang Q,Li H,Wen Z,Zhang H,Zhang H

    更新日期:2013-01-01 00:00:00

  • Topical Diclofenac Solution for Osteoarthritis of the Knee: An Updated Meta-Analysis of Randomized Controlled Trials.

    abstract::This study was performed to assess the efficacy and safety of a topical diclofenac solution in patients with knee osteoarthritis (OA). PubMed, Embase, Cochrane Library, Web of Science, and Scopus databases were searched for randomized controlled trials until June 2020. The WOMAC pain, stiffness, physical function subs...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/1758071

    authors: Ling T,Li JJ,Xu RJ,Wang B,Ge WH

    更新日期:2020-11-24 00:00:00

  • Gender Dysphoria: Bioethical Aspects of Medical Treatment.

    abstract::Gender affirmation surgery remains one of the greatest challenges in transgender medicine. In recent years, there have been continuous discussions on bioethical aspects in the treatment of persons with gender dysphoria. Gender reassignment is a difficult process, including not only hormonal treatment with possible sur...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2018/9652305

    authors: Bizic MR,Jeftovic M,Pusica S,Stojanovic B,Duisin D,Vujovic S,Rakic V,Djordjevic ML

    更新日期:2018-06-13 00:00:00

  • Association between the MMP-1-1607 1G/2G Polymorphism and Osteoarthritis Risk: A Systematic Review and Meta-Analysis.

    abstract:Background:Osteoarthritis (OA) is a common disease characterized by articular cartilage degeneration and secondary hyperosteogenesis. Genetic factors are associated with the occurrence of OA. While several studies have shown that the matrix metalloproteinase-1- (MMP-1-) 1607 1G/2G (rs1799750) polymorphism may be relate...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2020/5190587

    authors: Liu J,Wang G,Peng Z

    更新日期:2020-05-20 00:00:00

  • Identification and Analysis of Novel Biomarkers Involved in Chromophobe Renal Cell Carcinoma by Integrated Bioinformatics Analyses.

    abstract::In renal cell carcinoma, chromophobe renal cell carcinoma (ChRCC) is a distinct subtype, whose clinical manifestations often lack specificity, and the molecular mechanisms of ChRCC tumorigenesis remain generally vague. The target of this study was to discover novel biomarkers involved in ChRCC by integrated bioinforma...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/2671281

    authors: Zhang W,Xu Y,Zhang J,Wu J

    更新日期:2020-02-07 00:00:00

  • Morphometric and statistical analysis of the palmaris longus muscle in human and non-human primates.

    abstract::The palmaris longus is considered a phylogenetic degenerate metacarpophalangeal joint flexor muscle in humans, a small vestigial forearm muscle; it is the most variable muscle in humans, showing variation in position, duplication, slips and could be reverted. It is frequently studied in papers about human anatomical v...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2014/178906

    authors: Aversi-Ferreira RA,Bretas RV,Maior RS,Davaasuren M,Paraguassú-Chaves CA,Nishijo H,Aversi-Ferreira TA

    更新日期:2014-01-01 00:00:00

  • Identification of Sp1 as a Transcription Activator to Regulate Fibroblast Growth Factor 21 Gene Expression.

    abstract::Fibroblast growth factor 21 (FGF21) is a metabolic hormone with multiple beneficial effects on lipid and glucose homeostasis. Previous study demonstrated that FGF21 might be one of the Sp1 target genes. However, the transcriptional role of Sp1 on FGF21 in adipose tissue and liver has not been reported. In this study, ...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2017/8402035

    authors: Chen S,Li H,Zhang J,Jiang S,Zhang M,Xu Y,Dong K,Yang Y,Fang Q,Jia W

    更新日期:2017-01-01 00:00:00

  • Syringobulbia in Patients with Chiari Malformation Type I: A Systematic Review.

    abstract::This study aimed to summarize the clinical features, diagnosis, and treatment of Chiari malformation type I- (CM-1-) associated syringobulbia. We performed a literature review of CM-1-associated syringobulbia in PubMed, Ovid MEDLINE, and Web of Science databases. Our concerns were the clinical features, radiologic pre...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2019/4829102

    authors: Shen J,Shen J,Huang K,Wu Y,Pan J,Zhan R

    更新日期:2019-03-19 00:00:00

  • The Signaling Pathways Involved in the Antiatherosclerotic Effects Produced by Chinese Herbal Medicines.

    abstract::Cardiovascular diseases (CVDs) are considered to be the predominant cause of death in the world. Chinese herb medicines (CHMs) have been widely used for the treatment of CVDs in Asian countries for thousands of years. One reason of high efficacy of CHMs in treating CVDs is attributed to their inhibition in atheroscler...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2018/5392375

    authors: Lu L,Sun X,Qin Y,Guo X

    更新日期:2018-06-13 00:00:00

  • A Brief Review on the Neuroprotective Mechanisms of Vitexin.

    abstract::The neural dysfunction is triggered by cellular and molecular events that provoke neurotoxicity and neural death. Currently, neurodegenerative diseases are increasingly common, and available treatments are focused on relieving symptoms. Based on the above, in this review we describe the participation of vitexin in the...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2018/4785089

    authors: Lima LKF,Pereira SKS,Junior RDSS,Santos FPDS,Nascimento AS,Feitosa CM,Figuerêdo JS,Cavalcante ADN,Araújo ECDC,Rai M

    更新日期:2018-12-05 00:00:00

  • Curcumin Downregulates Phosphate Carrier and Protects against Doxorubicin Induced Cardiomyocyte Apoptosis.

    abstract::Aim. To explore the effects of curcumin on phosphate carrier (PiC) and its role in protection against doxorubicin induced myocyte toxicity. Methods. Using H9c2 cell line, the cardiotoxic effect of doxorubicin and its mitigation by curcumin were studied. H9c2 cells were cultured with doxorubicin and/or curcumin at vari...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2016/1980763

    authors: Junkun L,Erfu C,Tony H,Xin L,Sudeep KC,Mingliang Z,Yanqin W,XiangQian Q

    更新日期:2016-01-01 00:00:00

  • Serum Osteocalcin and Testosterone Concentrations in Adult Males with or without Primary Osteoporosis: A Meta-Analysis.

    abstract::Osteocalcin (Ocn) and testosterone play important roles in male skeleton. However, the concentrations of serum osteocalcin and testosterone have never been systematically compared between populations with and without primary male osteoporosis, a common skeletal disorder in adult males. We searched the PubMed, Embase, ...

    journal_title:BioMed research international

    pub_type: 杂志文章,meta分析

    doi:10.1155/2017/9892048

    authors: Liu ZY,Yang Y,Wen CY,Rong LM

    更新日期:2017-01-01 00:00:00

  • Antioxidant delivery pathways in the anterior eye.

    abstract::Tissues in the anterior segment of the eye are particular vulnerable to oxidative stress. To minimise oxidative stress, ocular tissues utilise a range of antioxidant defence systems which include nonenzymatic and enzymatic antioxidants in combination with repair and chaperone systems. However, as we age our antioxidan...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2013/207250

    authors: Umapathy A,Donaldson P,Lim J

    更新日期:2013-01-01 00:00:00

  • Alpha-Tocopherol Serum Levels Are Increased in Caucasian Women with Uterine Fibroids: A Pilot Study.

    abstract::Uterine fibroids (UFs) are benign tumors of the reproductive tract, arising from smooth muscle cells of the uterus. Steroid hormones, estrogen, and progesterone are considered to be the most important links in the pathophysiology of UFs. Alpha-tocopherol (AT) is the most active form of vitamin E. What is important as ...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/6793726

    authors: Ciebiera M,Szymańska-Majchrzak J,Sentkowska A,Kilian K,Rogulski Z,Nowicka G,Jakiel G,Tomaszewski P,Włodarczyk M

    更新日期:2018-07-24 00:00:00

  • Identification and Functional Mechanism of Novel Angiotensin I Converting Enzyme Inhibitory Dipeptides from Xerocomus badius Cultured in Shrimp Processing Waste Medium.

    abstract::ACE inhibitory dipeptides from Xerocomus badius fermented shrimp processing waste were isolated with ethanol, macroporous resin, chloroform, and Sephadex G-10 in sequence and identified by LC-MS/MS system coupled with electrospray ionization source. Molecular docking was performed for exploring the mechanism of their ...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/5089270

    authors: Gao X,Li X,Yan P,Sun R,Kan G,Zhou Y

    更新日期:2018-05-08 00:00:00

  • EEG-Based Computer Aided Diagnosis of Autism Spectrum Disorder Using Wavelet, Entropy, and ANN.

    abstract::Autism spectrum disorder (ASD) is a type of neurodevelopmental disorder with core impairments in the social relationships, communication, imagination, or flexibility of thought and restricted repertoire of activity and interest. In this work, a new computer aided diagnosis (CAD) of autism ‎based on electroencephalogra...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2017/9816591

    authors: Djemal R,AlSharabi K,Ibrahim S,Alsuwailem A

    更新日期:2017-01-01 00:00:00

  • Resistance Status to the Insecticides Temephos, Deltamethrin, and Diflubenzuron in Brazilian Aedes aegypti Populations.

    abstract::Insecticides are still largely applied in public health to control disease vectors. In Brazil, organophosphates (OP) and pyrethroids (PY) are used against Aedes aegypti for years. Since 2009 Insect Growth Regulators (IGR) are also employed in the control of larvae. We quantified resistance to temephos (OP), deltamethr...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2016/8603263

    authors: Bellinato DF,Viana-Medeiros PF,Araújo SC,Martins AJ,Lima JB,Valle D

    更新日期:2016-01-01 00:00:00

  • Efficacy of Sucralfate-Combined Quadruple Therapy on Gastric Mucosal Injury Induced by Helicobacter pylori and Its Effect on Gastrointestinal Flora.

    abstract:Background:This study explored the therapeutic efficacy of standard triple therapy combined with sucralfate suspension gel as well as the mechanisms of action in mouse models of H. pylori infection. Materials and Methods:C57BL/6J mice were randomly divided into 5 groups: NC (natural control), HP (H. pylori infection),...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/4936318

    authors: Teng G,Liu Y,Wu T,Wang W,Wang H,Hu F

    更新日期:2020-08-31 00:00:00

  • Primary Cilia Blockage Promotes the Malignant Behaviors of Hepatocellular Carcinoma via Induction of Autophagy.

    abstract::Primary cilia are organelles protruding from cell surface into environment that function in regulating cell cycle and modulating cilia-related signal. Primary ciliogenesis and autophagy play important roles in tumorigenesis. However, the functions and interactions between primary cilia and autophagy in hepatocellular ...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2019/5202750

    authors: Liu L,Sheng JQ,Wang MR,Gan Y,Wu XL,Liao JZ,Tian DA,He XX,Li PY

    更新日期:2019-10-02 00:00:00

  • Relationship between serum total cholesterol level and serum biochemical bone turnover markers in healthy pre- and postmenopausal women.

    abstract:BACKGROUND:The presence of common risk factors suggests that there is a relationship between osteoporosis and cardiovascular disease, possibly via dyslipidemia and inflammation. We investigated the relationships among the lipid profile, the inflammation marker high-sensitivity C-reactive protein (hsCRP), bone turnover ...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2014/398397

    authors: Jeong TD,Lee W,Choi SE,Kim JS,Kim HK,Bae SJ,Chun S,Min WK

    更新日期:2014-01-01 00:00:00

  • Health Effects of 12 Weeks of Team-Sport Training and Fitness Training in a Community Health Centre for Sedentary Men with Lifestyle Diseases.

    abstract::This study compares the effects of team-sport training, for sedentary men with lifestyle diseases, with fitness training in a pragmatic set-up in a community health centre (CHC). Thirty-two men in the fitness group (FiG) and 36 men in the team-sport group (TsG) completed the training and trained for 60-90 min, two tim...

    journal_title:BioMed research international

    pub_type: 临床试验,杂志文章

    doi:10.1155/2018/1571807

    authors: Møller TK,Nielsen TT,Andersen R,Lundager I,Hansen HF,Ottesen L,Krustrup P,Randers MB

    更新日期:2018-05-15 00:00:00

  • Prevalence and Clinical Features of Atopic Dermatitis in China.

    abstract::Background. The epidemiology of atopic dermatitis (AD) in Chinese outpatients is yet to be clarified. Objectives. To investigate population-based prevalence and clinical features of AD in Chinese outpatients. Methods. A multicenter cross-sectional study was conducted in outpatients with eczema or dermatitis from 39 te...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2016/2568301

    authors: Wang X,Li LF,Zhao DY,Shen YW

    更新日期:2016-01-01 00:00:00

  • Decorin-Mediated Inhibition of Human Trophoblast Cells Proliferation, Migration, and Invasion and Promotion of Apoptosis In Vitro.

    abstract::Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion. Decorin (DCN) has been proved to be a decidua-derived TGF-binding prote...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/201629

    authors: Zou Y,Yu X,Lu J,Jiang Z,Zuo Q,Fan M,Huang S,Sun L

    更新日期:2015-01-01 00:00:00