Abstract:
:Infective endocarditis (IE) is a life-threatening disease that is associated with high morbidity and mortality. Its long-term prognosis strongly depends on a timely and optimized antibiotic treatment. Therefore, identification of the causative pathogen is crucial and currently based on blood cultures followed by characterization and susceptibility testing of the isolate. However, antibiotic treatment starting prior to blood sampling or IE caused by fastidious or intracellular microorganisms may cause negative culture results. Here we investigate the additional diagnostic value of broad-range PCR in combination with direct sequencing on resected heart tissue or swabs in patients with tissue or swab culture-negative IE in a routine clinical setting. Sensitivity, specificity, and positive and negative predictive values of broad-range PCR from diagnostic material in our patients were 33.3%, 76.9%, 90.9%, and 14.3%, respectively. We identified a total of 20 patients (21.5%) with tissue or culture-negative IE who profited by the additional application of broad-range PCR. We conclude that broad-range PCR on resected heart tissue or swabs is an important complementary diagnostic approach. It should be seen as an indispensable new tool for both the therapeutic and diagnostic management of culture-negative IE and we thus propose its possible inclusion in Duke's diagnostic classification scheme.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Maneg D,Sponsel J,Müller I,Lohr B,Penders J,Madlener K,Hunfeld KPdoi
10.1155/2016/7923874subject
Has Abstractpub_date
2016-01-01 00:00:00pages
7923874eissn
2314-6133issn
2314-6141journal_volume
2016pub_type
杂志文章abstract::Unipotent spermatogonial stem cells (SSCs) can be transformed into ESC-like cells that exhibit pluripotency in vitro. However, except for mouse models, their characterization and their origins have remained controversies in other models including humans. This controversy has arisen primarily from the lack of the direc...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2013/143028
更新日期:2013-01-01 00:00:00
abstract:Purpose:To evaluate the relationships between objectively measured physical activity and physical fitness among preschool children. Methods:A total of 346 participants (201 boys and 145 girls) aged 3.5-5.5 years (M = 4.5 yr, SD = 0.47) from Shanghai, China, completed physical fitness assessments, including triceps ski...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/9314026
更新日期:2017-01-01 00:00:00
abstract::Tyrosine kinase inhibitors (TKI) belong to a new class of molecular multitargeted anticancer therapy which targets different growth factor receptors and hence attenuates cancer cell survival and growth. Since their introduction as adjunct treatment for renal cell carcinoma and gastrointestinal stromal tumors (GIST), a...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2013/725410
更新日期:2013-01-01 00:00:00
abstract::Apoptosis of both brain neurons and peripheral blood leukocyte is believed to be an important biomarker for evaluating the functional status of Parkinson's disease (PD). However, their correlation remains unknown. A better understanding of the pathophysiology of neurodegeneration is essential for the treatment and pre...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/635923
更新日期:2014-01-01 00:00:00
abstract:INTRODUCTION:Our study aims to determine the prevalence of nicotine dependence and investigate the effect of nicotine dependence on psychopathology among schizophrenia patients. METHODS:A cross-sectional study was carried out in an outpatient psychiatric clinic at a general hospital in Malaysia. 180 recruited subjects...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2015/730291
更新日期:2015-01-01 00:00:00
abstract::Destruction of extracellular matrix (ECM) is one of the basic steps of tumor invasion and metastasis. Matrix metalloproteinase (MMP) 9, a kind of zinc-ion-dependent endopeptidase, can degrade almost all protein components in the ECM, destroy the histological barrier of tumor cell invasion, and play a key role in tumor...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/4350783
更新日期:2020-04-15 00:00:00
abstract::Lung cancer is one of the leading triggers for cancer death worldwide. In this study, the relationship of the aberrantly methylated and differentially expressed genes in lung adenocarcinoma (LUAD) with cancer prognosis was investigated, and 5 feature genes were identified eventually. Specifically, we firstly downloade...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/1807089
更新日期:2020-09-25 00:00:00
abstract::Neuroendocrine tumors (NETs) are tumors originated from neuroendocrine cells in the body. The localization and the detection of the extent of NETs are important for diagnosis and treatment, which should be individualized according to the tumor type, burden, and symptoms. Molecular imaging of NETs with high sensitivity...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2013/102819
更新日期:2013-01-01 00:00:00
abstract::This study was performed to assess the efficacy and safety of a topical diclofenac solution in patients with knee osteoarthritis (OA). PubMed, Embase, Cochrane Library, Web of Science, and Scopus databases were searched for randomized controlled trials until June 2020. The WOMAC pain, stiffness, physical function subs...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/1758071
更新日期:2020-11-24 00:00:00
abstract::Gender affirmation surgery remains one of the greatest challenges in transgender medicine. In recent years, there have been continuous discussions on bioethical aspects in the treatment of persons with gender dysphoria. Gender reassignment is a difficult process, including not only hormonal treatment with possible sur...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2018/9652305
更新日期:2018-06-13 00:00:00
abstract:Background:Osteoarthritis (OA) is a common disease characterized by articular cartilage degeneration and secondary hyperosteogenesis. Genetic factors are associated with the occurrence of OA. While several studies have shown that the matrix metalloproteinase-1- (MMP-1-) 1607 1G/2G (rs1799750) polymorphism may be relate...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2020/5190587
更新日期:2020-05-20 00:00:00
abstract::In renal cell carcinoma, chromophobe renal cell carcinoma (ChRCC) is a distinct subtype, whose clinical manifestations often lack specificity, and the molecular mechanisms of ChRCC tumorigenesis remain generally vague. The target of this study was to discover novel biomarkers involved in ChRCC by integrated bioinforma...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/2671281
更新日期:2020-02-07 00:00:00
abstract::The palmaris longus is considered a phylogenetic degenerate metacarpophalangeal joint flexor muscle in humans, a small vestigial forearm muscle; it is the most variable muscle in humans, showing variation in position, duplication, slips and could be reverted. It is frequently studied in papers about human anatomical v...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/178906
更新日期:2014-01-01 00:00:00
abstract::Fibroblast growth factor 21 (FGF21) is a metabolic hormone with multiple beneficial effects on lipid and glucose homeostasis. Previous study demonstrated that FGF21 might be one of the Sp1 target genes. However, the transcriptional role of Sp1 on FGF21 in adipose tissue and liver has not been reported. In this study, ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/8402035
更新日期:2017-01-01 00:00:00
abstract::This study aimed to summarize the clinical features, diagnosis, and treatment of Chiari malformation type I- (CM-1-) associated syringobulbia. We performed a literature review of CM-1-associated syringobulbia in PubMed, Ovid MEDLINE, and Web of Science databases. Our concerns were the clinical features, radiologic pre...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/4829102
更新日期:2019-03-19 00:00:00
abstract::Cardiovascular diseases (CVDs) are considered to be the predominant cause of death in the world. Chinese herb medicines (CHMs) have been widely used for the treatment of CVDs in Asian countries for thousands of years. One reason of high efficacy of CHMs in treating CVDs is attributed to their inhibition in atheroscler...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2018/5392375
更新日期:2018-06-13 00:00:00
abstract::The neural dysfunction is triggered by cellular and molecular events that provoke neurotoxicity and neural death. Currently, neurodegenerative diseases are increasingly common, and available treatments are focused on relieving symptoms. Based on the above, in this review we describe the participation of vitexin in the...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2018/4785089
更新日期:2018-12-05 00:00:00
abstract::Aim. To explore the effects of curcumin on phosphate carrier (PiC) and its role in protection against doxorubicin induced myocyte toxicity. Methods. Using H9c2 cell line, the cardiotoxic effect of doxorubicin and its mitigation by curcumin were studied. H9c2 cells were cultured with doxorubicin and/or curcumin at vari...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/1980763
更新日期:2016-01-01 00:00:00
abstract::Osteocalcin (Ocn) and testosterone play important roles in male skeleton. However, the concentrations of serum osteocalcin and testosterone have never been systematically compared between populations with and without primary male osteoporosis, a common skeletal disorder in adult males. We searched the PubMed, Embase, ...
journal_title:BioMed research international
pub_type: 杂志文章,meta分析
doi:10.1155/2017/9892048
更新日期:2017-01-01 00:00:00
abstract::Tissues in the anterior segment of the eye are particular vulnerable to oxidative stress. To minimise oxidative stress, ocular tissues utilise a range of antioxidant defence systems which include nonenzymatic and enzymatic antioxidants in combination with repair and chaperone systems. However, as we age our antioxidan...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2013/207250
更新日期:2013-01-01 00:00:00
abstract::Uterine fibroids (UFs) are benign tumors of the reproductive tract, arising from smooth muscle cells of the uterus. Steroid hormones, estrogen, and progesterone are considered to be the most important links in the pathophysiology of UFs. Alpha-tocopherol (AT) is the most active form of vitamin E. What is important as ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/6793726
更新日期:2018-07-24 00:00:00
abstract::ACE inhibitory dipeptides from Xerocomus badius fermented shrimp processing waste were isolated with ethanol, macroporous resin, chloroform, and Sephadex G-10 in sequence and identified by LC-MS/MS system coupled with electrospray ionization source. Molecular docking was performed for exploring the mechanism of their ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/5089270
更新日期:2018-05-08 00:00:00
abstract::Autism spectrum disorder (ASD) is a type of neurodevelopmental disorder with core impairments in the social relationships, communication, imagination, or flexibility of thought and restricted repertoire of activity and interest. In this work, a new computer aided diagnosis (CAD) of autism based on electroencephalogra...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/9816591
更新日期:2017-01-01 00:00:00
abstract::Insecticides are still largely applied in public health to control disease vectors. In Brazil, organophosphates (OP) and pyrethroids (PY) are used against Aedes aegypti for years. Since 2009 Insect Growth Regulators (IGR) are also employed in the control of larvae. We quantified resistance to temephos (OP), deltamethr...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/8603263
更新日期:2016-01-01 00:00:00
abstract:Background:This study explored the therapeutic efficacy of standard triple therapy combined with sucralfate suspension gel as well as the mechanisms of action in mouse models of H. pylori infection. Materials and Methods:C57BL/6J mice were randomly divided into 5 groups: NC (natural control), HP (H. pylori infection),...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/4936318
更新日期:2020-08-31 00:00:00
abstract::Primary cilia are organelles protruding from cell surface into environment that function in regulating cell cycle and modulating cilia-related signal. Primary ciliogenesis and autophagy play important roles in tumorigenesis. However, the functions and interactions between primary cilia and autophagy in hepatocellular ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/5202750
更新日期:2019-10-02 00:00:00
abstract:BACKGROUND:The presence of common risk factors suggests that there is a relationship between osteoporosis and cardiovascular disease, possibly via dyslipidemia and inflammation. We investigated the relationships among the lipid profile, the inflammation marker high-sensitivity C-reactive protein (hsCRP), bone turnover ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/398397
更新日期:2014-01-01 00:00:00
abstract::This study compares the effects of team-sport training, for sedentary men with lifestyle diseases, with fitness training in a pragmatic set-up in a community health centre (CHC). Thirty-two men in the fitness group (FiG) and 36 men in the team-sport group (TsG) completed the training and trained for 60-90 min, two tim...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2018/1571807
更新日期:2018-05-15 00:00:00
abstract::Background. The epidemiology of atopic dermatitis (AD) in Chinese outpatients is yet to be clarified. Objectives. To investigate population-based prevalence and clinical features of AD in Chinese outpatients. Methods. A multicenter cross-sectional study was conducted in outpatients with eczema or dermatitis from 39 te...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/2568301
更新日期:2016-01-01 00:00:00
abstract::Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion. Decorin (DCN) has been proved to be a decidua-derived TGF-binding prote...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/201629
更新日期:2015-01-01 00:00:00