Abdominal Aortic Aneurysm-Associated MicroRNA-516a-5p Regulates Expressions of Methylenetetrahydrofolate Reductase, Matrix Metalloproteinase-2, and Tissue Inhibitor of Matrix Metalloproteinase-1 in Human Abdominal Aortic Vascular Smooth Muscle Cells.

Abstract:

BACKGROUND:MicroRNAs (miRNAs or miRs) have been highlighted to be involved in abdominal aortic aneurysm (AAA) with the emergence of recent miRNA microarray profiling studies. miR-516a-5p has been shown to be significantly overexpressed in vascular smooth muscle cells (VSMCs) from human AAA tissues from our previous microarray study, suggesting its crucial association with AAA. In addition, further bioinformatics analysis predicted methylenetetrahydrofolate reductase (MTHFR), which regulates homocysteine (Hcy) metabolism and is proposed to be a risk gene for AAA formation and to be the downregulation target of miR-516a-5p. However, the pathogenic role of miR-516a-5p in VSMCs for AAA formation remains unresolved. This study aims to investigate the role of miR-516a-5p in human VSMCs for AAA pathogenesis. METHODS:miR-516a-5p was stably overexpressed and knocked down in VSMCs explant cultured from human abdominal aortic tissues by means of lentiviral system. The MTHFR protein expression was first examined by Western blotting. In addition, the protein expressions of several key components involved in AAA pathogenic features are as follows: matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitor of matrix metalloproteinase (TIMP)-1 and TIMP-2 for elastin degradation; collagen type 1 alpha 1 for compensatory collagen synthesis; monocyte chemoattractant protein-1 for inflammation, were also evaluated. Apoptotic level of VSMCs was examined by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. RESULTS:Results showed that protein expression of MTHFR was significantly downregulated on miR-516a-5p overexpression (P < 0.05) in VSMCs, whereas it was significantly upregulated on miR-516a-5p knockdown (P < 0.05). Of all the AAA key components investigated, only MMP-2 and TIMP-1 protein expressions were found altered. A significant increase in MMP-2 (P < 0.05) and decrease in TIMP-1 (P < 0.05) expressions were observed on miR-516a-5p overexpression in VSMCs. Apoptosis was not promoted on miR-516a-5p overexpression or knockdown in VSMCs. CONCLUSIONS:Our findings suggested that miR-516a-5p may regulate MTHFR, MMP-2, and TIMP-1 expressions in human VSMCs, possibly promoting the disruption of Hcy metabolism and proteolytic degradation of elastin for AAA formation.

journal_name

Ann Vasc Surg

authors

Chan CYT,Cheuk BLY,Cheng SWK

doi

10.1016/j.avsg.2016.10.062

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

263-273

eissn

0890-5096

issn

1615-5947

pii

S0890-5096(17)30377-1

journal_volume

42

pub_type

杂志文章
  • Simulated Wound Assessment Using Digital Planimetry versus Three-Dimensional Cameras: Implications for Clinical Assessment.

    abstract:BACKGROUND:Clinical management of wounds can benefit from objective measures of response to treatment. Wound surface area and volume are objective measures of wound healing. Using a synthetic wound model, we compare the accuracy and reproducibility of 2 commercially available 3-dimensional (3D) cameras against planimet...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2016.10.029

    authors: Williams KJ,Sounderajah V,Dharmarajah B,Thapar A,Davies AH

    更新日期:2017-05-01 00:00:00

  • Plication as primary treatment of steal syndrome in arteriovenous fistulas.

    abstract::Steal syndrome is an uncommon complication following hemodialysis access. Options for management include fistula ligation, banding, and distal revascularization with interval ligation (DRIL). Plication is another technique that is simple yet infrequently reported. We have adopted plication as the procedure of choice f...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2008.08.009

    authors: Yaghoubian A,de Virgilio C

    更新日期:2009-01-01 00:00:00

  • Vascular growth in ischemic limbs: a review of mechanisms and possible therapeutic stimulation.

    abstract::Stimulation of vascular growth to treat limb ischemia is promising, and early results obtained from uncontrolled clinical trials using angiogenic agents, e.g., vascular endothelial growth factor, led to high expectations. However, negative results from recent placebo-controlled trials warrant further research. Here, c...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章,评审

    doi:10.1016/j.avsg.2008.02.017

    authors: van Weel V,van Tongeren RB,van Hinsbergh VW,van Bockel JH,Quax PH

    更新日期:2008-07-01 00:00:00

  • Clinical outcomes after endovascular therapy among hemodialysis patients with peripheral artery disease stratified by the primary kidney disease.

    abstract:OBJECTIVES:The objective of this study was to investigate the clinical characteristics of hemodialysis patients with peripheral artery disease (PAD) and the outcomes after endovascular therapy (EVT) in such patients stratified by the primary kidney disease. METHODS:This retrospective observational study evaluated 142 ...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2020.11.046

    authors: Iiya M,Hikita H,Yoshikawa H,Abe F,Tsujihata S,Ito N,Kanno Y,Hishikari K,Murai T,Takahashi A,Sasano T

    更新日期:2021-01-05 00:00:00

  • Radiation-induced rupture of the superficial femoral artery: a case report.

    abstract:BACKGROUND:Radiation-induced necrosis and arteriopathy are well-known iatrogenic pathologic entities. However, radiation-induced rupture of the superficial femoral artery is an exceptional life-threatening event. REPORT:We hereby report a case of radiation-induced rupture of the superficial femoral artery that was suc...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2014.12.034

    authors: Dahdah J,Farha G,Karam L,Kassabian E,Slaba S,Tabet G

    更新日期:2015-07-01 00:00:00

  • Internal iliac artery branch stent grafting for aortoiliac aneurysms using the Apollo branched device.

    abstract::The association of aortic and common iliac artery aneurysms requires a special strategy to achieve distal seal during the endovascular exclusion of abdominal aortic aneurysms. Coil embolization of the internal iliac artery before the placement of a bifurcated endograft limb into the external iliac artery is a usual op...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2009.08.014

    authors: Massière B,von Ristow A,Cury J,Gress M,Vescovi A,Marques M

    更新日期:2010-04-01 00:00:00

  • The relationship between gestational age and compliance in human umbilical vein and its possible application in vascular grafting.

    abstract::The aim of this study was to provide a theoretical basis, using biomechanical properties, for the clinical application of human umbilical vein (HUV) as material for vascular grafting. This was a nonrandomized, non-controlled in vitro study. The experiment was conducted in the Laboratory of Medical Biomechanics, Yunyan...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1007/s10016-006-9020-4

    authors: Li W,Huang T,Zeng Y,Yao Z

    更新日期:2006-03-01 00:00:00

  • Repair of Chronic Aneurysmal Aortic Dissection Using a Stent Graft and an Amplatzer® Vascular Plug: A Case Study.

    abstract::We report a case in which a stent graft and an Amplatzer® vascular plug (AVP) were effective for the treatment of chronic aneurysmal aortic dissection. The patient was a 52-year-old man. At 45 years of age, he developed acute aortic dissection, for which he underwent surgery 4 times with prosthetic graft replacement i...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2016.06.032

    authors: Kanaoka Y,Ohki T,Ozawa H

    更新日期:2017-02-01 00:00:00

  • Hybrid Repair of an Intrathoracic Bilobed Subclavian Artery Aneurysm.

    abstract::Intrathoracic subclavian artery aneurysms are extremely rare, <1% of all aneurysms. The formation of these aneurysms is often multifactorial, with the most common contributing factor being atherosclerosis. This case report describes a right subclavian artery aneurysm that required a surgical hybrid approach. These ope...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2018.12.106

    authors: Gillen J,Tse L,Berg A,Dardik H,Bernik T

    更新日期:2019-08-01 00:00:00

  • Abdominal aortic aneurysm rupture with vertebral erosion presenting with severe refractory back pain in Behçet's disease.

    abstract::Behçet's disease is a chronic recurrent inflammatory disorder that affects many organs. A 48-year-old man with known Behçet's disease was admitted to the emergency department with aggravating severe back pain and left lower quadrant pain. A contained rupture of infrarenal abdominal aortic aneurysm (AAA) was discovered...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2009.05.011

    authors: Ahn HJ,Kwon SH,Park HC

    更新日期:2010-02-01 00:00:00

  • Mycotic abdominal aortic aneurysm after adjuvant therapy with bacillus Calmette-Guérin in patients with urothelial bladder cancer: a rare but misinterpreted complication.

    abstract::Bacillus Calmette-Guérin (BCG) therapy is often associated with side effects. The most feared is disseminated sepsis that may occur rarely with the development of mycotic aortic aneurysms. Twenty cases of patients treated with intravesical BCG complicated by mycotic abdominal aortic aneurysm have been reported in the ...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2015.01.036

    authors: Leo E,Molinari AL,Rossi G,Ferrari SA,Terzi A,Lorenzi G

    更新日期:2015-08-01 00:00:00

  • Occlusion of the aorta and inferior vena cava in a patient with circulating anticoagulants.

    abstract::Large-vessel occlusion is uncommon in association with antiphospholipid antibodies. This report describes the first case involving occlusion of the infrarenal abdominal aorta and inferior vena cava in a 46-year-old woman with circulating lupus-like anticoagulants who presented with subacute lower extremity ischemia. T...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1007/s10016-001-0231-4

    authors: DiCenta I,Fadel E,Mussot S,Paul JF,Rémuzon G,Dartevelle P

    更新日期:2002-05-01 00:00:00

  • Primary aortoduodenal fistula and Q fever: an underrecognized association?

    abstract::We report a rare case of primary aortoduodenal fistula (ADF) secondary to a Coxiella burnetii (Q fever) infection in a patient with an abdominal aortic aneurysm. A review of the available literature on the vascular complications of Q fever is presented. Q fever should be suspected in vascular patients with close anima...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1007/s100169910046

    authors: Mejia A,Toursarkissian B,Hagino RT,Myers JG,Sykes MT

    更新日期:2000-05-01 00:00:00

  • Distal bypass for limb salvage: should the contralateral great saphenous vein be harvested?

    abstract::The great saphenous vein (GSV) is unusable in 20% of cases. The best alternative in these cases is the GSV of the contralateral lower limb (CLL). However, many surgeons are reluctant to harvest the contralateral GSV for fear that it might be needed for future treatment of critical ischemia in the CLL. The purpose of t...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1007/s10016-006-9089-9

    authors: Robin C,Lermusiaux P,Bleuet F,Martinez R

    更新日期:2006-11-01 00:00:00

  • Biomolecular mechanisms in varicose veins development.

    abstract::Varicose veins (VVs) can be described as tortuous and dilated palpable veins, which are more than 3 mm in diameter. They are one of the clinical presentations of chronic venous disorders, which are a significant cause of morbidity. The prevalence of VVs has been estimated at 25-33% in women and 10-20% in men and is st...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章,评审

    doi:10.1016/j.avsg.2014.10.009

    authors: Segiet OA,Brzozowa-Zasada M,Piecuch A,Dudek D,Reichman-Warmusz E,Wojnicz R

    更新日期:2015-02-01 00:00:00

  • Bronchial artery aneurysm treated with video-assisted thoracoscopic surgery.

    abstract::Bronchial artery aneurysms (BAA) are a rarely noted. Asymptomatic cases of BAA are very rare. Most patients are correctly diagnosed only after BAA ruptures. The conventional treatment includes aneurysm resection through thoracotomy or transcatheter arterial embolization. In the present report, we describe a case of an...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2013.12.021

    authors: Nakamura R,Inage Y,Tsuchiya A,Kato T,Yuzawa K,Koizumi M,Ueki H

    更新日期:2014-07-01 00:00:00

  • Prediction of compartment syndrome based on analysis of biochemical parameters.

    abstract:BACKGROUND:The possibility of predicting severe compartment syndrome using simple biochemical parameters was evaluated in a single-center study of 55 patients who presented with acute femoral embolism and who were treated with open surgical embolectomy. METHODS:Parameters related to tissue damage and oxidative metabol...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2012.12.006

    authors: Mitas P,Vejrazka M,Hruby J,Spunda R,Pecha O,Lindner J,Spacek M

    更新日期:2014-01-01 00:00:00

  • Evaluation of thrombogenicity of fluoropassivated polyester patches following carotid endarterectomy.

    abstract::The use of a patch after carotid endarterectomy (CE) is recommended to reduce the incidence of restenosis. Most studies on this subject report the implantation of saphenous vein or PTFE patches, because polyester has always been considered to be a thrombogenic material. The purpose of this study was to evaluate the th...

    journal_title:Annals of vascular surgery

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1007/s100160010129

    authors: Pedrini L,Dondi M,Magagnoli A,Magnoni F,Pisano E,Del Giudice E,Santoro M

    更新日期:2001-11-01 00:00:00

  • The influence of study design on the evaluation of ruptured abdominal aortic aneurysm treatment.

    abstract:BACKGROUND:The best strategy in the treatment for ruptured abdominal aortic aneurysm (RAAA) is an ongoing matter of debate. Differently from several retrospective studies, recent randomized controlled trials (RCTs) failed to demonstrate the superiority of endovascular repair (EVAR) over open repair (OPEN). The aim of t...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章,meta分析,评审

    doi:10.1016/j.avsg.2014.03.017

    authors: Pini R,Faggioli G,Longhi M,Mauro R,Freyrie A,Gargiulo M,Gallitto E,Mascoli C,Stella A

    更新日期:2014-08-01 00:00:00

  • Safety and effectiveness of endovascular therapy for claudication in octogenarians.

    abstract:BACKGROUND:Advanced age (≥ 80 years) has been associated with adverse outcomes after lower extremity bypass for critical limb ischemia (CLI), but endovascular therapy (ET) is reported to have comparable safety across age groups. Here, we assess the safety and effectiveness of advanced age on outcomes after ET for lifes...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2014.07.028

    authors: Jones DW,Siracuse JJ,Graham A,Connolly PH,Sedrakyan A,Schneider DB,Meltzer AJ

    更新日期:2015-01-01 00:00:00

  • Magnetic resonance imaging reveals edema-like changes not only subcutaneously, but also in muscle tissue after femoropopliteal bypass surgery.

    abstract:BACKGROUND:The pathophysiological mechanisms that induce postrevascularization edema after femoropopliteal bypass surgery are not completely understood. Reperfusion-associated injury to revascularized tissue and damage to lymphatic structures are both likely to play a role. Aim of this study was to study edema formatio...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2011.04.010

    authors: te Slaa A,Tetteroo E,Mulder PG,Ho GH,Vos LD,Moll FL,van der Laan L

    更新日期:2012-02-01 00:00:00

  • The histology of seeded PTFE grafts in humans.

    abstract::To define the histology of PTFE femoral-popliteal grafts seeded with enzymatically-derived endothelium, we examined light level and selected scanning electron micrographs of 20 graft biopsies. Thirteen grafts were chronically occluded and the midgraft samples had a thin lining of fibrin with scattered erythrocytes and...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/S0890-5096(06)62000-1

    authors: Herring MB,LeGrand DR

    更新日期:1989-04-01 00:00:00

  • Surgery on varicose veins in the early Ottoman period performed by Serefeddin Sabuncuoğlu.

    abstract::Serefeddin Sabuncuoğlu, a pioneer of surgery, is known to be the author of first illustrated surgery textbook, Cerrahiyyetu'l Haniyye (Imperial Surgery), which was written in Turkish in 1465 AD at the age of 80 years. The purpose of this article is to describe his contributions to varicose vein surgery. In addition to...

    journal_title:Annals of vascular surgery

    pub_type: 传,历史文章,杂志文章

    doi:10.1007/s10016-003-0012-3

    authors: Darçin OT,Andaç MH

    更新日期:2003-07-01 00:00:00

  • Rupture of the Remaining Renal Artery Aneurysm While Waiting for Stage 2 Surgery for a Patient with Bilateral Renal Artery Aneurysms and Multiple Aneurysms.

    abstract::A 21-year-old man was referred to our department for treatment of multiple aneurysms, including bilateral renal artery aneurysms. He was clinically diagnosed with Loeys-Dietz syndrome. So, we developed a 2-stage surgical strategy. The first surgery involved reconstructing the right renal artery and replacing part of t...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2018.10.032

    authors: Kozaki S,Miyamoto S,Shuto T,Tanaka H,Wada T,Anai H

    更新日期:2019-05-01 00:00:00

  • The Advantages of Using Intraoperative Indocyanine Green Fluorescence Angiography for Upper Extremity Arteriovenous Malformation: A Case Report.

    abstract::Vascular anomaly is a general term that includes all vascular malformations, vascular tumors, and other congenital vascular defects. Vascular malformation is the most common term, and it describes blood vessels that are abnormally formed at birth. Vascular malformations can develop in any part of the body. The most co...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2019.02.040

    authors: Akbayrak H,Çiçek ÖF,Orhan A,Büyükateş M

    更新日期:2019-08-01 00:00:00

  • Early Results of Supporting Free Flap Coverage of Mangled Lower Extremities with Long Saphenous Arteriovenous Loop Grafts.

    abstract:BACKGROUND:The ability to salvage the mangled lower extremity is both technically challenging and time consuming. It requires the collaborative efforts among multiple surgical specialties in addition to comprehensive post-traumatic wound follow-up. Our institution has integrated a dynamic effort among these specialists...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2020.07.056

    authors: Brumberg RS,Kaelin LD,Derosier LC,Hutchinson H

    更新日期:2020-08-12 00:00:00

  • Perioperative Outcomes of Minimally Invasive Aortic Arch Reconstruction with Branched Grafts Through a Partial Upper Sternotomy.

    abstract:BACKGROUND:Ministernotomy has been advocated over recent years as an alternative technique for different cardiovascular surgical procedures to reduce the surgical trauma associated with conventional full sternotomy. In recent years, several reports have addressed minimally invasive approaches to the proximal aorta and ...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2019.10.078

    authors: Iba Y,Yamada A,Kurimoto Y,Hatta E,Maruyama R,Miura S

    更新日期:2020-05-01 00:00:00

  • Transthoracic endoscopy for upper thoracic chemical sympathectomy.

    abstract::Beginning in April 1989, we have performed eight upper thoracic chemical sympathectomies by transthoracic endoscopy. The indications were occlusive arterial disease in four patients and Raynaud's syndrome and palmar hyperhidrosis in two patients each. Transthoracic endoscopy was performed under general anesthesia, thr...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1007/BF02008799

    authors: Bardaxoglou E,Reigner B,Enon B,Tolstuchow N,Lescalié F,Peret M,Chevalier JM

    更新日期:1992-07-01 00:00:00

  • Aneurysmectomy with arterial reconstruction of renal artery aneurysms in the endovascular era: a safe, effective treatment for both aneurysm and associated hypertension.

    abstract:BACKGROUND:Renal artery aneurysms (RAAs) represent a rare vascular pathology with an estimated incidence of <1%. Although an endovascular approach is being increasingly used to treat RAAs, we hypothesized that open surgical repair of RAA, specifically via aneurysmectomy with arterial reconstruction (AAR), is a safe, ef...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章

    doi:10.1016/j.avsg.2009.07.030

    authors: Chandra A,O'Connell JB,Quinones-Baldrich WJ,Lawrence PF,Moore WS,Gelabert HA,Jimenez JC,Rigberg DA,DeRubertis BG

    更新日期:2010-05-01 00:00:00

  • The increased use of computed tomography angiography and magnetic resonance angiography as the sole imaging modalities prior to infrainguinal bypass has had no effect on outcomes.

    abstract:BACKGROUND:Angiography remains the gold standard imaging modality before infrainguinal bypass. Computed tomography angiography (CTA) and magnetic resonance angiography (MRA) have emerged as noninvasive alternatives for preoperative imaging. We sought to examine contemporary trends in the utilization of CTA and MRA as i...

    journal_title:Annals of vascular surgery

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.avsg.2015.03.039

    authors: Shue B,Damle RN,Flahive J,Kalish JA,Stone DH,Patel VI,Schanzer A,Baril DT

    更新日期:2015-08-01 00:00:00