Abstract:
:The transcription cycle can be roughly divided into three stages: initiation, elongation, and termination. Understanding the molecular events that regulate all these stages requires a dynamic view of the underlying processes. The development of techniques to visualize and quantify transcription in single living cells has been essential in revealing the transcription kinetics. They have revealed that (a) transcription is heterogeneous between cells and (b) transcription can be discontinuous within a cell. In this review, we discuss the progress in our quantitative understanding of transcription dynamics in living cells, focusing on all parts of the transcription cycle. We present the techniques allowing for single-cell transcription measurements, review evidence from different organisms, and discuss how these experiments have broadened our mechanistic understanding of transcription regulation.
journal_name
Annu Rev Biophysjournal_title
Annual review of biophysicsauthors
Lenstra TL,Rodriguez J,Chen H,Larson DRdoi
10.1146/annurev-biophys-062215-010838subject
Has Abstractpub_date
2016-07-05 00:00:00pages
25-47eissn
1936-122Xissn
1936-1238journal_volume
45pub_type
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