Cell-Free Synthesis of Macromolecular Complexes.

Abstract:

:Cell-free protein synthesis based on E. coli cell extracts has been described for the first time more than 50 years ago. To date, cell-free synthesis is widely used for the preparation of toxic proteins, for studies of the translation process and its regulation as well as for the incorporation of artificial or labeled amino acids into a polypeptide chain. Many efforts have been directed towards establishing cell-free expression as a standard method for gene expression, with limited success. In this chapter we will describe the state-of-the-art of cell-free expression, extract preparation methods and recent examples for successful applications of cell-free synthesis of macromolecular complexes.

journal_name

Adv Exp Med Biol

authors

Botte M,Deniaud A,Schaffitzel C

doi

10.1007/978-3-319-27216-0_6

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

79-95

eissn

0065-2598

issn

2214-8019

journal_volume

896

pub_type

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