Abstract:
:The dearth of new antibiotics in the face of widespread antimicrobial resistance makes developing innovative strategies for discovering new antibiotics critical for the future management of infectious disease. Understanding the genetics and evolution of antibiotic producers will help guide the discovery and bioengineering of novel antibiotics. We discovered an isolate in Alaskan boreal forest soil that had broad antimicrobial activity. We elucidated the corresponding antimicrobial natural products and sequenced the genome of this isolate, designated Streptomyces sp. 2AW. This strain illustrates the chemical virtuosity typical of the Streptomyces genus, producing cycloheximide as well as two other biosynthetically unrelated antibiotics, neutramycin, and hygromycin A. Combining bioinformatic and chemical analyses, we identified the gene clusters responsible for antibiotic production. Interestingly, 2AW appears dissimilar from other cycloheximide producers in that the gene encoding the polyketide synthase resides on a separate part of the chromosome from the genes responsible for tailoring cycloheximide-specific modifications. This gene arrangement and our phylogenetic analyses of the gene products suggest that 2AW holds an evolutionarily ancestral lineage of the cycloheximide pathway. Our analyses support the hypothesis that the 2AW glutaramide gene cluster is basal to the lineage wherein cycloheximide production diverged from other glutarimide antibiotics. This study illustrates the power of combining modern biochemical and genomic analyses to gain insight into the evolution of antibiotic-producing microorganisms.
journal_name
Front Microbioljournal_title
Frontiers in microbiologyauthors
Stulberg ER,Lozano GL,Morin JB,Park H,Baraban EG,Mlot C,Heffelfinger C,Phillips GM,Rush JS,Phillips AJ,Broderick NA,Thomas MG,Stabb EV,Handelsman Jdoi
10.3389/fmicb.2016.00573subject
Has Abstractpub_date
2016-05-03 00:00:00pages
573issn
1664-302Xjournal_volume
7pub_type
杂志文章abstract::Milk spoilage is caused by the presence of proteolytic enzymes produced by Pseudomonas spp. during storage at low temperatures. The aim of this study was to identify Pseudomonas spp. in raw milk and investigate their associated proteolytic properties at low temperatures. Raw milk samples (n = 87) were collected from 8...
journal_title:Frontiers in microbiology
pub_type: 杂志文章
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更新日期:2019-07-24 00:00:00
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abstract::The practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In conseq...
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journal_title:Frontiers in microbiology
pub_type: 杂志文章,评审
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abstract::Over the past decades, antimicrobial resistance (AMR) has been recognized as one of the most serious threats to public health. Although originally considered a problem to human health, the emerging crisis of AMR requires a "One Health" approach, considering human, animal, and environmental reservoirs. In this regard, ...
journal_title:Frontiers in microbiology
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pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章,评审
doi:10.3389/fmicb.2014.00462
更新日期:2014-09-02 00:00:00
abstract::Retrospective studies involving the screening of frozen stored collections of samples are commonplace when a new threat emerges, but it has been demonstrated that the freeze-thaw process can affect bacterial viability. The study of colistin-resistant bacteria in human and animal samples is an example of this issue. In...
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
doi:10.3389/fmicb.2015.00400
更新日期:2015-05-06 00:00:00
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doi:10.3389/fmicb.2019.03015
更新日期:2020-01-10 00:00:00
abstract::Recently, controllable, targeted proteolysis has emerged as one of the most promising new strategies to study essential genes and otherwise toxic mutations. One of the principal limitations preventing the wider adoption of this approach is due to the lack of easily identifiable species-specific degrons that can be use...
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pub_type: 杂志文章
doi:10.3389/fmicb.2017.02572
更新日期:2017-12-19 00:00:00
abstract::Salicylic acid (SA) plays an important role in regulating plant defense responses against pathogens. However, pathogens have evolved ways to manipulate plant SA-mediated defense signaling. Fusarium graminearum causes Fusarium head blight (FHB) and reduces crop yields and quality by producing various mycotoxins. In thi...
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pub_type: 杂志文章
doi:10.3389/fmicb.2018.03219
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
doi:10.3389/fmicb.2014.00789
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journal_title:Frontiers in microbiology
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章,评审
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doi:10.3389/fmicb.2019.01002
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pub_type: 杂志文章,评审
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abstract::Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-associated in OMVs c...
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doi:10.3389/fmicb.2018.01837
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pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
doi:10.3389/fmicb.2015.01065
更新日期:2015-10-08 00:00:00
abstract::Microbiome research has increased dramatically in recent years, driven by advances in technology and significant reductions in the cost of analysis. Such research has unlocked a wealth of data, which has yielded tremendous insight into the nature of the microbial communities, including their interactions and effects, ...
journal_title:Frontiers in microbiology
pub_type: 杂志文章,评审
doi:10.3389/fmicb.2020.00136
更新日期:2020-02-19 00:00:00