Abstract:
:Ricin is a toxin that could potentially be used as a bioweapon. We identified anti-ricin A chain antibodies by sequencing the antibody repertoire from immunized mice and by selecting high affinity antibodies using yeast surface display. These methods led to the isolation of multiple antibodies with high (sub-nanomolar) affinity. Interestingly, the antibodies identified by the 2 independent approaches are from the same clonal lineages, indicating for the first time that yeast surface display can identify native antibodies. The new antibodies represent well-characterized reagents for biodefense diagnostics and therapeutics development.
journal_name
MAbsjournal_title
mAbsauthors
Wang B,Lee CH,Johnson EL,Kluwe CA,Cunningham JC,Tanno H,Crooks RM,Georgiou G,Ellington ADdoi
10.1080/19420862.2016.1190059subject
Has Abstractpub_date
2016-08-01 00:00:00pages
1035-44issue
6eissn
1942-0862issn
1942-0870journal_volume
8pub_type
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