Inhibitory effect of MyoD on the proliferation of breast cancer cells.

Abstract:

:Skeletal muscle is rich in lymphatic vessels, with an abundant blood supply, and it is an infrequent site of cancer metastasis. Previous studies have demonstrated that enhanced secretion of MyoD may occur when skeletal muscle is injured or becomes cancerous. It was hypothesized that MyoD may act as an endogenous cytokine to inhibit the proliferation of cancer cells. To verify the possible effect of this protein on tumor cell proliferation, C2C12 mouse skeletal muscle cells and 4T1 mouse breast cancer cells were co-cultured using embedded Transwell plates. Following co-culture, cell cycle analysis revealed that C2C12 muscle cells were able to inhibit the proliferation of the breast cancer cells. Subsequently, MyoD was silenced in C2C12 cells to assess its effect on 4T1 cell proliferation. Following co-culture with MyoD-silenced cells, a 5-ethynyl-20-deoxyuridine assay indicated that MyoD silencing prevented the reduction in proliferation of 4T1 cells induced by untransfected C2C12 cells. In summary, the results indicated that MyoD inhibits the proliferation of breast cancer cells and may be a tumor suppressor factor.

journal_name

Oncol Lett

journal_title

Oncology letters

authors

Cai C,Qin X,Wu Z,Shen Q,Yang W,Zhang S,Duan J,Liang F,Liu C

doi

10.3892/ol.2016.4448

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

3589-3596

issue

6

eissn

1792-1074

issn

1792-1082

pii

OL-0-0-4448

journal_volume

11

pub_type

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