Abstract:
:This study was undertaken to determine if structural alterations of the bulk chromatin and the amount of protein associated with the nuclear matrix in cerebellar neurons depend on radiation dose and a cell's state of oxygenation. After irradiation with 2.5 to 25.0 Gy under both aerobic and anoxic conditions, the sensitivity of the neuronal chromatin to m. nuclease digestion increase linearly with dose up to about 5 Gy, beyond which there was no further increase. The same increase in accessibility of chromatin to micrococcal nuclease digestion was observed when neuronal nuclei were irradiated at 4 degrees C. Neuronal nuclei were stained with propidium iodide (PI) for DNA and with fluorescein isothiocyanate (FITC) for protein, both before and after complete digestion with DNase I, and analyzed by flow cytometry. There was no change in either the PI (P greater than 0.4) or the FITC (P greater than 0.9) fluorescence of undigested nuclei after irradiation. For the DNase I digested nuclei, the PI fluorescence was unchanged after irradiation (P greater than 0.4), but the FITC fluorescence increased significantly (P less than 0.02). This increase in the FITC fluorescence was linear with dose up to about 5 Gy, beyond which there was no further increase. The flow cytometry results from DNase I digested nuclei were identical for neurons irradiated under aerobic or anoxic conditions, indicating that this phenomenon is oxygen independent. This increase in FITC fluorescence after irradiation was inhibited at ice-cold temperatures and probably reflects an increase in protein content at the nuclear matrix that requires metabolism. This may explain our previously observed resistance of nuclear matrix-associated DNA to digestion by DNase I. This protein increase at the nuclear matrix appears to follow "saturation" kinetics identical to that previously reported for repair of DNA strand breaks in cerebellar neurons. However, the exact molecular nature of this process and its role in DNA repair or cell survival remains to be determined.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Jaberaboansari A,Landis MR,Wallen CA,Wheeler KTsubject
Has Abstractpub_date
1989-07-01 00:00:00pages
57-72issue
1eissn
0033-7587issn
1938-5404journal_volume
119pub_type
杂志文章abstract::Previous studies showed that a 6-week pretreatment of rats with testosterone plus estradiol enhanced the recovery of spermatogenesis 9 weeks after gamma irradiation, resulting in a dose-modifying factor (DMF) of about 2. To test whether the effect of the hormone treatment was mediated through changes in oxygen tension...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::Nuclear protein and DNA content of HeLa cells was determined as a function of time following hyperthermia by staining isolated nuclei with two fluorescent dyes: fluorescein isothiocyanate (FITC) for protein content and propidium iodide (PI) for DNA content. Bivariate FITC and PI histograms were obtained by flow cytome...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::In this study, we investigated microvascular perfusion status, changes to fat content and fatty acid composition in the bone marrow of rat femurs after total-body irradiation by quantitative permeability parameters of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and ex vivo high-resolution magic angl...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14865.1
更新日期:2018-02-01 00:00:00
abstract::Experiments were performed with three different cell lines, mouse fibroblast LM cells, HeLa S3 cells, and Ehrlich Ascites Tumor (EAT) cells, to establish the possible importance of hyperthermic-induced alterations in cellular K+ content in the mechanism of cell killing by heat. At different time points after the hyper...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1987-02-01 00:00:00
abstract::Trp53 heterozygous mice are radiation-sensitive and cancer-prone. Groups of 7-8-week-old female Trp53 heterozygous mice were exposed to 4 Gy of 60Co gamma radiation at high (0.5 Gy/min) or low (0.5 mGy/min) dose rate. Other groups received 10 or 100 mGy at low dose rate 24 h prior to the 4-Gy dose. Tumor frequency and...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3190
更新日期:2004-07-01 00:00:00
abstract::These studies examined the effects of X radiation and interleukin 3 (IL-3), which is an effective cytokine for the generation of megakaryocytopoiesis from X-irradiated hematopoietic stem/progenitor cells, on the terminal process of human megakaryocytopoiesis and thrombopoiesis. Mature megakaryocytes were induced by cu...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1519.1
更新日期:2009-09-01 00:00:00
abstract::An analytical expression is developed for calculating the average energy deposited by an ion in a volume with dimensions less than the range of the secondary electrons produced. The expression is obtained by including two additional terms in an energy-restricted linear-energy-transfer equation. The usual energy-restri...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::The nature of the primary biochemical defect in the human radiosensitive and cancer-prone syndrome, ataxia telangiectasia (AT), has remained obscure despite many efforts to elucidate it. In this study, AT complementation group D cells and a nearly isogenic corrected AT-hamster hybrid derivative have been analyzed for ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::Dose assessment after radiological disasters is imperative to decrease mortality through rationally directed medical intervention. Our goal was to identify biomarkers capable of qualitative (nonirradiated/irradiated) and/or quantitative (dose) assessment of radiation exposure. Using real-time quantitative PCR, biodosi...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2419.1
更新日期:2011-07-01 00:00:00
abstract::To ensure the high-fidelity transmission by reproductive cells of genetic information from generation to generation, cells have evolved surveillance systems to eliminate genomic lesions by inducing cell suicide and/or DNA repair. In this report, gamma-ray-induced cell death was investigated using the medaka fish, Oryz...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2002)157[0386:acsisf]2.0.co;2
更新日期:2002-04-01 00:00:00
abstract::Telomeres cap the ends of chromosomes, preventing end-to-end fusions and subsequent chromosome instability. Here we used a telomerase knockout model to investigate whether telomerase participates in the processes of DNA break repair by de novo synthesis of telomere repeats at broken chromosome ends (chromosome healing...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3203
更新日期:2004-08-01 00:00:00
abstract::Triple negative breast cancer (TNBC) is an aggressive disease with a high risk of recurrence and death. Here, we present a novel strategy to enhance the radiotherapy of TNBC by combining gold nanoparticles (AuNPs) with pentamidine, a clinically approved anti-parasitic agent with anti-cancer properties. The radiosensit...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14315.1
更新日期:2016-05-01 00:00:00
abstract::In an effort to identify proteomic changes that may be useful for radiation biodosimetry, human cells of hematological origin were treated with ionizing radiation or mock-irradiated and then harvested at different times after treatment. Protein lysates were generated from these cells and evaluated by Western blotting ...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1638.1
更新日期:2009-05-01 00:00:00
abstract::Late radiation damage to pig skin has been assessed at 104 weeks after exposure to sources of 90Sr/90Y (Emax 2.2 MeV) and 170Tm (Emax 0.9 MeV). Damage was assessed from measurements of dermal thickness in histological sections of irradiated skin and was compared with that of unirradiated skin to establish the relative...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::Radiation-induced cell death is thought to be caused by nuclear DNA damage that cannot be repaired. However, in this study we found that a delayed increase of mitochondrial reactive oxygen species (ROS) is responsible for some of the radiation-induced cell death in normal human fibroblast cells. We have previously rep...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR13772.1
更新日期:2015-04-01 00:00:00
abstract::Gleevec, a selective tyrosine kinase inhibitor, retarded the growth of anaplastic thyroid cancer cell lines in vitro and in vivo through selective inhibition of ABL tyrosine kinase activity. In the present study, we investigated the ability of Gleevec to modulate the in vitro and in vivo radiation response of anaplast...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3466.1
更新日期:2006-01-01 00:00:00
abstract::The exposure of peripheral blood mononuclear cells (PBMCs) was performed in a 10-T static magnetic field. Without lymphocyte stimulation, there were no significant differences in the viability of the exposed and unexposed CD4(+) T cells, CD8(+) T cells, B cells, and natural killer (NK) cells. The expression of Th1 typ...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2003)159[0775:eotsmf]2.0.co;2
更新日期:2003-06-01 00:00:00
abstract::The efficacy of photodynamic therapy is dependent upon the optical dose rate or upon the fractionation schedule on the light. These effects are thought to be limited by the time required for oxygen diffusion from the capillaries, since this therapy can consume oxygen faster than it can be supplied to tissues distant f...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.2307/3579621
更新日期:1997-05-01 00:00:00
abstract::Whole-body exposure of mice to 2 Gy gamma radiation results in an increase in the specific and total activities of esterase and alkaline phosphatase found in the supernatant of liver and kidney. This increase developed at 1 h, reached a maximum at 24 h, and then declined at 48 h after irradiation. The increase in the ...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1987-09-01 00:00:00
abstract::Exposure to ionizing radiation alone or combined with traumatic tissue injury is a crucial life-threatening factor in nuclear and radiological incidents. Radiation injuries occur at the molecular, cellular, tissue and systemic levels; their mechanisms, however, remain largely unclear. Exposure to radiation combined wi...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14647.1
更新日期:2017-11-01 00:00:00
abstract::We have manipulated the extracellular Ca2+ concentration (1.8 mM in normal Dulbecco's modified Eagle's medium) to test whether the resulting effect on intracellular free Ca2+ homeostasis was similar in heat-sterilized and nonheated mouse NIH-3T3 cells. The responsiveness of the intracellular free Ca2+ concentration to...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::We have investigated tumor response to low-dose-rate irradiation from an implanted 125I source alone or in conjunction with intratumoral drug administration. The drug (cis-DDP or 5-FU) was incorporated homogeneously into the co-polymer CPP-SA, 20:80, and the polymer/drug rods were implanted in the RIF-1 fibrosarcomas ...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3204
更新日期:2004-07-01 00:00:00
abstract::In higher eukaryotes, DNA double-strand breaks (DSBs) induced by ionizing radiation activate checkpoints that delay progression through the cell cycle. Compared to delays in other phases of the cell cycle, delays induced in G(2) are longer and frequently correlate with resistance to killing by radiation. Therefore, mo...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR2165.1
更新日期:2010-10-01 00:00:00
abstract::These studies were carried out to examine the capacity of alpha-difluoromethylornithine (DFMO) to modulate cell proliferation and apoptosis in cells of squamous cell carcinomas (SCCs) of the head and neck. Exposure of cells to DFMO (5 mM for 48 h) depleted intracellular putrescine and spermidine levels (greater than 5...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Human keratinocytes show a bystander effect when exposed to low doses of low-LET radiation. In this paper, data are presented showing a method of correcting the overall survival curve to enable analysis of the relative contributions of the bystander effect and the effect attributable to direct interaction of the radia...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)153[0508:rcobat]2.0.co;2
更新日期:2000-05-01 00:00:00
abstract::The relationships between DNA damage and the survival of murine bone marrow cells irradiated in situ were examined. Cell survival was assayed by the ability of bone marrow cells from irradiated mice to form colonies in vitro (CFU-C). DNA double-strand breaks (DSBs) were measured by neutral (nondenaturing) filter eluti...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::Human peripheral blood samples collected from three healthy human volunteers were exposed in vitro to pulsed-wave 2450 MHz radiofrequency (RF) radiation for 2 h. The RF radiation was generated with a net forward power of 21 W and transmitted from a standard gain rectangular antenna horn in a vertically downward direct...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)153[0479:pddihb]2.0.co;2
更新日期:2000-04-01 00:00:00
abstract::Takagaki, M., Ono, K., Masunaga, S-I., Kinashi, Y., Oda, Y., Miyatake, S-I., Hashimoto, N., Powell, W., Sood, A. and Spielvogel, B. F. Boronated Dipeptide Borotrimethylglycylphenylalanine as a Potential Boron Carrier in Boron Neutron Capture Therapy for Malignant Brain Tumors. Radiat. Res. 156, 118-122 (2001).A borona...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2001)156[0118:bdbaap]2.0.co;2
更新日期:2001-07-01 00:00:00
abstract::The acute effects of proton whole-body irradiation on the distribution and function of leukocyte populations in the spleen and blood were examined and compared to the effects of photons derived from a (60)Co gamma-ray source. Adult female C57BL/6 mice were exposed to a single dose (3 Gy at 0.4 Gy/min) of protons at sp...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2000)153[0587:aeowbp]2.0.co;2
更新日期:2000-05-01 00:00:00
abstract::Vascular change by the combined treatments of radiation and hyperthermia was studied. The legs of rats were irradiated with 2000 rad of X rays in a single dose and heated at 43.0 degrees C for 1 hr at various times after irradiation, and the blood flow and vascular volume in the skin and muscle were determined with 12...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1983-05-01 00:00:00