Design and optimization of aspartate N-acetyltransferase inhibitors for the potential treatment of Canavan disease.

Abstract:

:Canavan disease is a fatal neurological disorder caused by defects in the metabolism of N-acetyl-l-aspartate (NAA). Recent work has shown that the devastating symptoms of this disorder are correlated with the elevated levels of NAA observed in these patients, caused as a consequence of the inability of mutated forms of aspartoacylase to adequately catalyze its breakdown. The membrane-associated enzyme responsible for the synthesis of NAA, aspartate N-acetyltransferase (ANAT), has recently been purified and examined (Wang et al., Prot Expr Purif. 2016;119:11). With the availability, for the first time, of a stable and soluble form of ANAT we can now report the identification of initial inhibitors against this biosynthetic enzyme, obtained from the screening of several focused compound libraries. Two core structures of these moderate binding compounds have subsequently been optimized, with the most potent inhibitors in these series possessing sub-micromolar inhibition constants (Ki values) against ANAT. Slowing the production of NAA via the inhibition of ANAT will lower the elevated levels of this metabolite and can potentially serve as a treatment option to moderate the symptoms of Canavan disease.

journal_name

Bioorg Med Chem

authors

Thangavelu B,Mutthamsetty V,Wang Q,Viola RE

doi

10.1016/j.bmc.2016.11.060

subject

Has Abstract

pub_date

2017-02-01 00:00:00

pages

870-885

issue

3

eissn

0968-0896

issn

1464-3391

pii

S0968-0896(16)31091-4

journal_volume

25

pub_type

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