Abstract：
AIMS:This study's purpose was to evaluate the effect of ultrasound in air at a frequency of 0.04MHz and an intensity of 50mW/cm2 on neural differentiation of hBM-MSCs in vitro and on neurogenesis in an ischemic stroke model. MATERIALS AND METHODS:hBM-MSCs were exposed to 0.04MHz ultrasound and then compared with no exposed one in cell morphology, lactate dehydrogenase (LDH) activity, RT-PCR, and Western blot. In addition, we made stroke model mice by means of the photothrombosis (PT) method and these models were exposed to 0.04MHz ultrasound after hBM-MSCs injection. We compared with sham group in histological and immunohistochemical analysis and western blot. KEY FINDINGS:Ultrasound induced neural differentiation without cell death. In stroke models, inflammatory cells were observed around the infarct area in the Cell, Cell/Ultrasound group and the brain infarct volume in the Cell/Ultrasound group was smaller than in the sham group. Further, the expression of neural proteins in the Cell/Ultrasound group was increased relative to the sham group. SIGNIFICANCE:The present study showed that ultrasound promotes neural differentiation of hBM-MSC and neurogenesis in a mouse stroke model. This may be applicable as a therapeutic device with the aim of inducing neurogenesis following stroke.
journal_name
Life Scijournal_title
Life sciencesauthors
Cho SE,Kim YM,Jeong JS,Seo YKdoi
10.1016/j.lfs.2016.08.029subject
Has Abstractpub_date
2016-11-15 00:00:00pages
35-42eissn
0024-3205issn
1879-0631pii
S0024-3205(16)30500-8journal_volume
165pub_type
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