Cyanobacterium-Derived Extracellular Carbohydrate Polymer for the Controlled Delivery of Functional Proteins.

Abstract:

:The unicellular cyanobacterium Cyanothece sp. CCY 0110 is a highly efficient producer of extracellular polymeric substances (EPS), releasing up to 75% of the polymer to the culture medium. The carbohydrate polymer released to the medium (RPS) was previously isolated and characterized; it is composed of nine different monosaccharides including two uronic acids, and also containing peptides and sulfate groups. Here it is shown that the RPS spontaneously assembles with proteins at high concentrations leading to a phase transition. The proteins are released progressively and structurally intact near physiological conditions, primarily through the swelling of the polymer-protein matrix. The releasing kinetics of the proteins can be modulated through the addition of divalent cations, such as calcium. Notably, the polymer is not toxic to human dermal neonatal fibroblasts in vitro at RPS concentrations bellow 0.1 mg mL-1 . The results show that this polymer is a good candidate for the delivery of therapeutic macromolecules.

journal_name

Macromol Biosci

authors

Leite JP,Mota R,Durão J,Neves SC,Barrias CC,Tamagnini P,Gales L

doi

10.1002/mabi.201600206

subject

Has Abstract

pub_date

2017-02-01 00:00:00

issue

2

eissn

1616-5187

issn

1616-5195

journal_volume

17

pub_type

杂志文章
  • Study on Self-Assembled Well-Defined PEG Graft Copolymers as Efficient Drug-Loaded Nanoparticles for Anti-Inflammatory Therapy.

    abstract::The core-shell micelles in aqueous solutions were prepared at low CAC values (<0.1 mg · mL(-1) ) from amphiphilic graft copolymers consisting of poly(methyl methacrylate) backbone (120-165 units) with loosely grafted (17-40%) hydroxyl-capped PEG (9 vs 6 units in side chain) for drug loading. Hydrophobic indomethacin (...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201500189

    authors: Maksym-Bębenek P,Neugebauer D

    更新日期:2015-11-01 00:00:00

  • Polysaccharides and their derivatives for versatile tissue engineering application.

    abstract::Research and development in the design, synthesis, modification, evaluation, and characterization of polysaccharide-based bioactive polymeric materials for guiding and promoting new tissue in-growth is reviewed. Emphasis is given in this interdisciplinary field of tissue engineering (TE) with particular reference to b...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章,评审

    doi:10.1002/mabi.201200409

    authors: Khan F,Ahmad SR

    更新日期:2013-04-01 00:00:00

  • Novel biodegradable poly(1,8-octanediol malate) for annulus fibrosus regeneration.

    abstract::A novel elastic scaffold that simulates the deformability of annulus fibrosus (AF) and has good biocompatibility was developed. The scaffold was formed of a malic acid-based polyester poly(1,8-octanediol malate) (POM), which was synthesized by direct polycondensation. The tensile strength of POM gradually increased wi...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200700053

    authors: Wan Y,Feng G,Shen FH,Balian G,Laurencin CT,Li X

    更新日期:2007-11-12 00:00:00

  • Plasmid DNA microgels for a therapeutical strategy combining the delivery of genes and anticancer drugs.

    abstract::Plasmid DNA (pDNA) is encapsulated into biocompatible microgels by an inverse microemulsion polymerization method. Plasmid DNA and doxorubicin are successfully released from pDNA microgels and their release profiles are characterized by appropriate release models. The co-delivery of genes and drugs from the microgels ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201200096

    authors: Costa D,Valente AJ,Miguel MG,Queiroz J

    更新日期:2012-09-01 00:00:00

  • Injectable Hydrogels as Unique Platforms for Local Chemotherapeutics-Based Combination Antitumor Therapy.

    abstract::Different strategies of chemotherapeutics-based combination cancer therapy have presented enhanced antitumor efficiency and are widely used in clinical cancer treatments. However, several drawbacks of the systems for systemic administration, including low drug accumulation at tumor sites and significant systemic side ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章,评审

    doi:10.1002/mabi.201800240

    authors: Yu S,He C,Chen X

    更新日期:2018-12-01 00:00:00

  • Functionalized polystyrene nanoparticles trigger human dendritic cell maturation resulting in enhanced CD4+ T cell activation.

    abstract::Nanoparticles (NP) represent a promising tool for biomedical applications. Here, sulfonate- and phosphonate-functionalized polystyrene NP are analyzed for their interaction with human monocyte-derived dendritic cells (DC). Immature dendritic cells (iDC) display a higher time- and dose-dependent uptake of functionalize...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201200223

    authors: Frick SU,Bacher N,Baier G,Mailänder V,Landfester K,Steinbrink K

    更新日期:2012-12-01 00:00:00

  • Stereoblock poly(lactic acid): synthesis via solid-state polycondensation of a stereocomplexed mixture of poly(L-lactic acid) and poly(D-lactic acid).

    abstract::Stereoblock poly(lactic acid) consisting of D- and L-lactate stereosequences can be successfully synthesized by solid-state polycondensation of a 1:1 mixture of poly(L-lactic acid) and poly(D-lactic acid). In the first step, melt-polycondensation of L- and D-lactic acids is conducted to synthesize poly(L-lactic acid) ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200400121

    authors: Fukushima K,Furuhashi Y,Sogo K,Miura S,Kimura Y

    更新日期:2005-01-14 00:00:00

  • An in vivo study of the host response to starch-based polymers and composites subcutaneously implanted in rats.

    abstract::Implant failure is one of the major concerns in the biomaterials field. Several factors have been related to the fail but in general these biomaterials do not exhibit comparable physical, chemical or biological properties to natural tissues and ultimately, these devices can lead to chronic inflammation and foreign-bod...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200500010

    authors: Marques AP,Reis RL,Hunt JA

    更新日期:2005-08-12 00:00:00

  • Improved anti-inflammatory and pharmacokinetic properties for superoxide dismutase by chemical glycosidation with carboxymethylchitin.

    abstract::O-carboxymethylchitin (molecular weight = 1.07 x 10(5), degree of carboxymethylation = 80%, degree of N-acetylation = 91%) was chemically attached to superoxide dismutase by the formation of amide linkages through a carbodiimide catalyzed reaction. The glycosidated enzyme contained about 1.8 mole of polysaccharide per...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200400114

    authors: Valdivia A,Perez Y,Dominguez A,Caballero J,Gomez L,Schacht EH,Villalonga R

    更新日期:2005-02-23 00:00:00

  • Electrospun nanofibers as dressings for chronic wound care: advances, challenges, and future prospects.

    abstract::Chronic non-healing wounds show delayed and incomplete healing processes and in turn expose patients to a high risk of infection. Treatment currently focuses on dressings that prevent microbial infiltration and keep a balanced moisture and gas exchange environment. Antibacterial delivery from dressings has existed for...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章,评审

    doi:10.1002/mabi.201300561

    authors: Abrigo M,McArthur SL,Kingshott P

    更新日期:2014-06-01 00:00:00

  • Poly(ethylene glycol) grafting to poly(ether imide) membranes: influence on protein adsorption and thrombocyte adhesion.

    abstract::The chain length and end groups of linear PEG grafted on smooth surfaces is known to influence protein adsorption and thrombocyte adhesion. Here, it is explored whether established structure function relationships can be transferred to application relevant, rough surfaces. Functionalization of poly(ether imide) (PEI) ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201300309

    authors: Neffe AT,von Ruesten-Lange M,Braune S,Luetzow K,Roch T,Richau K,Jung F,Lendlein A

    更新日期:2013-12-01 00:00:00

  • Polymers for the cell-specific immobilisation of megakaryocytic cell lines.

    abstract::Primary human megakaryocytes, the precursor cells of platelets, are difficult to collect and cultivate. Polymers that enrich these cells without affecting their regulation or function are of interest for basic research as well as for cord blood transplantation purposes since co-transplantation of enriched megakaryocyt...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201200368

    authors: Hansen A,Corless S,Cleland A,Petrik J,Gilbert N,Bradley M

    更新日期:2013-04-01 00:00:00

  • Hybrid polyglycerols with long blood circulation: synthesis, biocompatibility, and biodistribution.

    abstract::Multifunctional polymers with defined structure and biocompatibility are critical to the development of drug delivery systems and bioconjugates. In this article, the synthesis, in vitro blood compatibility, cell viability, in vivo circulation, biodistribution, and clearance of hybrid copolymers based on linear and bra...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201400152

    authors: Imran ul-haq M,Lai BF,Kizhakkedathu JN

    更新日期:2014-10-01 00:00:00

  • Dual delivery of vascular endothelial growth factor and hepatocyte growth factor coacervate displays strong angiogenic effects.

    abstract::Controlled delivery of multiple growth factors (GFs) holds great potential for the clinical treatment of ischemic diseases and might be more therapeutically effective to reestablish vasculature than the provision of a single GF. Vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) are two poten...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201300486

    authors: Awada HK,Johnson NR,Wang Y

    更新日期:2014-05-01 00:00:00

  • pH-Triggered Self-Assembly of Cellulose Oligomers with Gelatin into a Double-Network Hydrogel.

    abstract::Multicomponent systems for self-assembled molecular gels provide huge opportunities to generate collective or new functions that are not inherent in individual single-component gels. However, gelation tends to require careful and complicated procedures, because, among a myriad of kinetically trapped structures related...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.202000187

    authors: Hata Y,Kojima T,Maeda T,Sawada T,Serizawa T

    更新日期:2020-09-01 00:00:00

  • POxylated Polyurea Dendrimers: Smart Core-Shell Vectors with IC50 Lowering Capacity.

    abstract::The design and preparation of highly efficient drug delivery platforms using green methodologies is at the forefront of nanotherapeutics research. POxylated polyurea dendrimers are efficiently synthesized using a supercritical-assisted polymerization in carbon dioxide. These fluorescent, pH-responsive and water-solubl...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201500131

    authors: Restani RB,Conde J,Pires RF,Martins P,Fernandes AR,Baptista PV,Bonifácio VD,Aguiar-Ricardo A

    更新日期:2015-08-01 00:00:00

  • Physicochemical Characterization of the Shell Composition of PBCA-Based Polymeric Microbubbles.

    abstract::Microbubbles (MB) are routinely used as contrast agents for ultrasound (US) imaging. In recent years, MB have also attracted interest as drug delivery systems. Soft-shelled lipidic MB tend to be more advantageous for US imaging, while hard-shelled polymeric MB appear to be more suitable for drug delivery purposes beca...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201700002

    authors: Appold L,Shi Y,Rütten S,Kühne A,Pich A,Kiessling F,Lammers T

    更新日期:2017-10-01 00:00:00

  • Mechanisms for Imparting Conductivity to Nonconductive Polymeric Biomaterials.

    abstract::Traditionally, conductive materials for electrodes are based on high modulus metals or alloys. Development of bioelectrodes that mimic the mechanical properties of the soft, low modulus tissues in which they are implanted is a rapidly expanding field of research. Many polymers exist that more closely match tissue mech...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章,评审

    doi:10.1002/mabi.201600057

    authors: Patton AJ,Poole-Warren LA,Green RA

    更新日期:2016-08-01 00:00:00

  • Multifunctional poly(Vinyl Amine)s bearing Azetidinium groups: one pot preparation in water and antimicrobial properties.

    abstract::A simple, robust one pot procedure for the preparation of waterborne multifunctional poly(vinyl amine)s (PVAms) is presented. By post-polymerization modification of PVAm with a bifunctional coupler and functional couplers cationic, reactive azetidinium groups, and alkyl chains are introduced in the side chains of PVAm...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201300576

    authors: Chattopadhyay S,Heine ET,Keul H,Möller M

    更新日期:2014-08-01 00:00:00

  • Multiple-Level Porous Polymer Monoliths with Interconnected Cellular Topology Prepared by Combining Hard Sphere and Emulsion Templating for Use in Bone Tissue Engineering.

    abstract::A combination of hard sphere and high internal phase emulsion templating gives a platform for synthesizing hierarchically porous polymers with a unique topology exhibiting interconnected spherical features on multiple levels. Polymeric spheres are fused by thermal sintering to create a 3D monolithic structure while an...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201700306

    authors: Paljevac M,Gradišnik L,Lipovšek S,Maver U,Kotek J,Krajnc P

    更新日期:2018-02-01 00:00:00

  • Characterization of the inhibition of enveloped virus infectivity by the cationic acrylate polymer eudragit E100.

    abstract::The antiviral effects of the cationic acrylate polymer E100 on a panel of lipid-enveloped viruses and the interactions involved are studied. The treatment of several common viruses with E100 induced a dose-dependent inhibition of the infectivity of viruses below the detection limit of the assays employed. Similarly, t...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200700102

    authors: Alasino RV,Bianco ID,Vitali MS,Zarzur JA,Beltramo DM

    更新日期:2007-09-11 00:00:00

  • Oxidative Epigallocatechin Gallate Coating on Polymeric Substrates for Bone Tissue Regeneration.

    abstract::Plant derived flavonoids have not been well explored in tissue engineering applications due to difficulties in efficient formulations with biomaterials for controlled presentation. Here, the authors report that surface coating of epigallocatechin gallate (EGCG) on polymeric substrates including poly (L-lactic acid) (P...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201800392

    authors: Madhurakkat Perikamana SK,Lee SM,Lee J,Ahmad T,Lee MS,Yang HS,Shin H

    更新日期:2019-04-01 00:00:00

  • Composite PLA/PEG/nHA/Dexamethasone Scaffold Prepared by 3D Printing for Bone Regeneration.

    abstract::3D printing has become an essential part of bone tissue engineering and attracts great attention for the fabrication of bioactive scaffolds. Combining this rapid manufacturing technique with chemical precipitation, biodegradable 3D scaffold composed of polymer matrix (polylactic acid and polyethylene glycol), ceramics...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201800068

    authors: Li X,Wang Y,Wang Z,Qi Y,Li L,Zhang P,Chen X,Huang Y

    更新日期:2018-06-01 00:00:00

  • Amylose's recognition of chirality in polylactides on formation of inclusion complexes in vine-twining polymerization.

    abstract::Amylose selectively includes poly(L-lactide) (PLLA) among the poly(lactide)s (PLAs) to produce an inclusion complex when the phosphorylase-catalyzed polymerization of α-D-glucose 1-phosphate is performed in the presence of PLLA, poly(D-lactide) (PDLA), or poly(DL-lactide) (PDLLA) (vine-twining polymerization). This re...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201100133

    authors: Kaneko Y,Ueno K,Yui T,Nakahara K,Kadokawa J

    更新日期:2011-10-10 00:00:00

  • Reinforcing silk scaffolds with silk particles.

    abstract::Silk fibroin is a useful protein polymer for biomaterials and tissue engineering. In this work, porogen leached scaffolds prepared from aqueous and HFIP silk solutions were reinforced through the addition of silk particles. This led to about 40 times increase in the specific compressive modulus and the yield strength ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200900358

    authors: Rajkhowa R,Gil ES,Kluge J,Numata K,Wang L,Wang X,Kaplan DL

    更新日期:2010-06-11 00:00:00

  • Non-woven fibrous materials with antibacterial properties prepared by tailored attachment of quaternized chitosan to electrospun mats from maleic anhydride copolymer.

    abstract::In order to impart antibacterial properties to microfibrous electrospun materials from styrene/maleic anhydride copolymers, quaternized chitosan derivatives (QCh) containing alkyl substituents of different chain lengths are covalently attached to the mats. A complete inhibition of the growth of bacteria, S. aureus (Gr...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201100178

    authors: Ignatova M,Petkova Z,Manolova N,Markova N,Rashkov I

    更新日期:2012-01-01 00:00:00

  • Cloning of poly(aspartic acid) (PAA) hydrolase-1 gene from Pedobacter sp. KP-2 and hydrolysis of thermally synthesized PAA by its gene product.

    abstract::Pedobacter sp. KP-2 can degrade and metabolize thermally synthesized alpha,beta-poly(D,L-aspartic acid) (tPAA), which contains 70% of unnatural beta-amide units, with high-molecular-weight. In this study, gene cloning and molecular characterization of PAA hydrolase-1 from KP-2 was carried out. Gene analysis reveals th...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200800106

    authors: Hiraishi T,Masuda E,Kanayama N,Nagata M,Doi Y,Abe H,Maeda M

    更新日期:2009-01-09 00:00:00

  • Suppression of cell and platelet adhesion to star-shaped 8-armed poly(ethylene glycol)-poly(L-lactide) block copolymer films.

    abstract::To explore the potential of a star-shaped 8-armed poly(ethylene glycol)35K-block-poly(L-lactide)37K (8-armed PEG35K-b-PLLA37K: M(n) of PEG = 35 000, M(n) of PLLA = 37 000) film as a novel bioabsorbable adhesion-prevention membrane, the water structure, surface contact angle, protein adsorption, and cell and platelet a...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200500249

    authors: Nagahama K,Ohya Y,Ouchi T

    更新日期:2006-06-16 00:00:00

  • Poly(amidoamine)-based dendrimer/siRNA complexation studied by computer simulations: effects of pH and generation on dendrimer structure and siRNA binding.

    abstract::In this work we report, compare and discuss the results obtained from fully atomistic molecular dynamics simulations of generations 4, 5, and 6 of PAMAM-based dendrimers having NH(3) and triethanolamine as cores, forming complexes with a short interfering RNA (siRNA) at different pH values and at physiological ionic ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201100276

    authors: Karatasos K,Posocco P,Laurini E,Pricl S

    更新日期:2012-02-01 00:00:00

  • Mastoid obliteration using three-dimensional composite scaffolds consisting of polycaprolactone/β-tricalcium phosphate/collagen nanofibers: an in vitro and in vivo study.

    abstract::Two different composite scaffolds, solid-freeform-fabricated PCL/β-TCP supplemented with and without collagen nanofibers are fabricated. These scaffolds are evaluated whether a combination of collagen nanofibers with PCL/β-TCP can promote osteogenesis in a mastoid obliteration. To assess the effects of the cellular ac...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201200393

    authors: Jang CH,Cho YB,Yeo MG,Kim GH

    更新日期:2013-05-01 00:00:00