Proteomics analysis of serum protein profiling in pancreatic cancer patients by DIGE: up-regulation of mannose-binding lectin 2 and myosin light chain kinase 2.


BACKGROUND:Pancreatic cancer has significant morbidity and mortality worldwide. Good prognosis relies on an early diagnosis. The purpose of this study was to develop techniques for identifying cancer biomarkers in the serum of patients with pancreatic cancer. METHODS:Serum samples from five individuals with pancreatic cancer and five individuals without cancer were compared. Highly abundant serum proteins were depleted by immuno-affinity column. Differential protein analysis was performed using 2-dimensional differential in-gel electrophoresis (2D-DIGE). RESULTS:Among these protein spots, we found that 16 protein spots were differently expressed between the two mixtures; 8 of these were up-regulated and 8 were down-regulated in cancer. Mass spectrometry and database searching allowed the identification of the proteins corresponding to the gel spots. Up-regulation of mannose-binding lectin 2 and myosin light chain kinase 2, which have not previously been implicated in pancreatic cancer, were observed. In an independent series of serum samples from 16 patients with pancreatic cancer and 16 non-cancer-bearing controls, increased levels of mannose-binding lectin 2 and myosin light chain kinase 2 were confirmed by western blot. CONCLUSIONS:These results suggest that affinity column enrichment and DIGE can be used to identify proteins differentially expressed in serum from pancreatic cancer patients. These two proteins 'mannose-binding lectin 2 and myosin light chain kinase 2' might be potential biomarkers for the diagnosis of the pancreatic cancer.


BMC Gastroenterol


BMC gastroenterology


Rong Y,Jin D,Hou C,Hu J,Wu W,Ni X,Wang D,Lou W




Has Abstract


2010-06-29 00:00:00










  • Reactive lymphoid hyperplasia of the liver in a patient with colon cancer: report of two cases.

    abstract:BACKGROUND:Reactive lymphoid hyperplasia (RLH) of the liver is very rarely reported, and we encountered two cases of RLH of the liver in a patient with colon cancer. CASE PRESENTATION:In the first case, a 77-year-old woman was admitted for the surgical removal of a ascending colon cancer. A hepatic tumor in the left l...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Takahashi H,Sawai H,Matsuo Y,Funahashi H,Satoh M,Okada Y,Inagaki H,Takeyama H,Manabe T

    更新日期:2006-09-12 00:00:00

  • Sustained serological and complete responses in HBeAg-positive patients treated with Peginterferon alfa-2b: a 6-year long-term follow-up of a multicenter, randomized, controlled trial in China.

    abstract:BACKGROUND:Pegylated interferon (PEG-IFN) alfa-2b is recommended for chronic hepatitis B (CHB). We aimed to investigate the sustainability of off-treatment responses among Chinese HBeAg-positive CHB patients treated with PEG-IFN alfa-2b from a randomized trial. METHODS:Eligible Chinese patients (n = 322) were followed...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Sun J,Ding H,Chen G,Wang G,Wei L,Zhang J,Xie Q,Wan M,Tang H,Chen S,Gao Z,Wang Y,Zhang D,Huang W,Sheng J,Ning Q,Yang D,Lu J,Pan C,Yang Y,Wang J,Sun C,Wang Q,Hou J

    更新日期:2019-05-02 00:00:00

  • The level and prevalence of depression and anxiety among patients with different subtypes of irritable bowel syndrome: a network meta-analysis.

    abstract:BACKGROUND:Irritable bowel syndrome (IBS) is a very common functional bowel disorder. However, the difference of depression and anxiety comorbidities among different IBS subtypes is still not well evaluated. This study aims to investigate the difference in the level and prevalence of depression and anxiety among health...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Hu Z,Li M,Yao L,Wang Y,Wang E,Yuan J,Wang F,Yang K,Bian Z,Zhong LLD

    更新日期:2021-01-07 00:00:00

  • Fluoroless intravascular ultrasound image-guided liver navigation in porcine models.

    abstract:BACKGROUND:An intravascular ultrasound catheter (IVUSc) was developed for intracardiac ultrasound to assess interventions with compelling results. However, intrahepatic vascular exploration was rarely tested and was always associated with X-ray techniques. The aim of this study was to demonstrate the feasibility to nav...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Urade T,Verde JM,García Vázquez A,Gunzert K,Pessaux P,Marescaux J,Giménez ME

    更新日期:2021-01-09 00:00:00

  • Sleep problems and functional disability in children with functional gastrointestinal disorders: an examination of the potential mediating effects of physical and emotional symptoms.

    abstract:BACKGROUND:Sleep disturbances are increasingly recognized as a common problem for children and adolescents with chronic pain conditions, but little is known about the prevalence, type, and impact of sleep problems in pediatric functional gastrointestinal disorders (FGIDs). The objectives of the current study were two-f...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Schurman JV,Friesen CA,Dai H,Danda CE,Hyman PE,Cocjin JT

    更新日期:2012-10-15 00:00:00

  • Simultaneous brain and lung metastases of pancreatic ductal adenocarcinoma after curative pancreatectomy: a case report and literature review.

    abstract:BACKGROUND:Pancreatic ductal adenocarcinoma (PDAC) rarely metastasizes to the brain; therefore, the features of brain metastasis of PDAC are still unknown. We encountered simultaneous metastases to the brain and lung in a PDAC patient after curative surgery. Case presentation A 68-year-old man with PDAC in the tail of ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Oka Y,Takano S,Kouchi Y,Furukawa K,Takayashiki T,Kuboki S,Suzuki D,Sakai N,Kagawa S,Hosokawa I,Mishima T,Konishi T,Kishimoto T,Ohtsuka M

    更新日期:2021-01-06 00:00:00

  • Clinical experiences and predictors of success of treatment with vedolizumab in IBD patients: a cohort study.

    abstract:BACKGROUND:Vedolizumab has become a standard treatment for the inflammatory bowel diseases ulcerative colitis (UC) and Crohn's disease (CD). However, there is an ongoing debate on the ideal individual treatment algorithms and means to predict treatment response are not routinely established. AIMS:We aimed to describe ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Mühl L,Becker E,Müller TM,Atreya R,Atreya I,Neurath MF,Zundler S

    更新日期:2021-01-22 00:00:00

  • Sensitivity is not an intrinsic property of a diagnostic test: empirical evidence from histological diagnosis of Helicobacter pylori infection.

    abstract:BACKGROUND:We aimed to provide empirical evidence of how spectrum effects can affect the sensitivity of histological assessment of Helicobacter pylori infection, which may contribute to explain the heterogeneity in prevalence estimates across populations with expectedly similar prevalence. METHODS:Cross-sectional eval...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Lunet N,Peleteiro B,Carrilho C,Figueiredo C,Azevedo A

    更新日期:2009-12-24 00:00:00

  • Applicability and precautions of use of liver injury biomarker FibroTest. A reappraisal at 7 years of age.

    abstract:BACKGROUND:FibroTest (FT) is a validated biomarker of fibrosis. To assess the applicability rate and to reduce the risk of false positives/negatives (RFPN), security algorithms were developed. The aims were to estimate the prevalence of RFPN and of proven failures, and to identify factors associated with their occurren...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Poynard T,Munteanu M,Deckmyn O,Ngo Y,Drane F,Messous D,Castille JM,Housset C,Ratziu V,Imbert-Bismut F

    更新日期:2011-04-14 00:00:00

  • IFI16 is involved in HBV-associated acute-on-chronic liver failure inflammation.

    abstract:BACKGROUND:Hepatitis B virus (HBV) is a hepatotropic DNA virus, and its DNA may be a potent inflammatory molecule. Interferon-inducible protein 16 (IFI16), a newly discovered DNA sensor, plays an important role in the process of inflammation in viral infections. Our study sought to identify a correlation between IFI16 ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Pang X,Li X,Mo Z,Huang J,Deng H,Lei Z,Zheng X,Feng Z,Xie D,Gao Z

    更新日期:2018-05-09 00:00:00

  • Gastric bacterial Flora in patients Harbouring Helicobacter pylori with or without chronic dyspepsia: analysis with matrix-assisted laser desorption ionization time-of-flight mass spectroscopy.

    abstract:BACKGROUND:The gastric microbiota has recently been implicated in the causation of organic/structural gastroduodenal diseases (gastric and duodenal ulcers, gastric cancer) in patients with Helicobacter pylori (H. pylori) infection. We aimed to ascertain, in patients harbouring H. pylori, the role of the gastric microbi...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Pereira V,Abraham P,Nallapeta S,Shetty A

    更新日期:2018-01-26 00:00:00

  • Serum trefoil factor 3 is a promising non-invasive biomarker for gastric cancer screening: a monocentric cohort study in China.

    abstract:BACKGROUND:The search for better non-invasive biomarkers for gastric cancer remains ongoing. We investigated the predictive power of serum trefoil factor (TFF) levels as biomarkers for gastric cancer in comparison with the pepsinogen (PG) test. METHODS:Patients with gastric cancer, chronic atrophic gastritis (CAG) or ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Huang Z,Zhang X,Lu H,Wu L,Wang D,Zhang Q,Ding H

    更新日期:2014-04-11 00:00:00

  • Liver disease and 30-day mortality after colorectal cancer surgery: a Danish population-based cohort study.

    abstract:BACKGROUND:Colorectal cancer (CRC) is common, with surgery as the main curative treatment. The prevalence of chronic liver disease has increased, but knowledge is limited on postoperative mortality in patients with liver disease who undergo CRC surgery. Hence, we examined 30-day mortality after CRC surgery in patients ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Montomoli J,Erichsen R,Christiansen CF,Ulrichsen SP,Pedersen L,Nilsson T,Sørensen HT

    更新日期:2013-04-15 00:00:00

  • Slug down-regulation by RNA interference inhibits invasion growth in human esophageal squamous cell carcinoma.

    abstract:BACKGROUND:Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive carcinomas of the gastrointestinal tract. We assessed the relevance of Slug in measuring the invasive potential of ESCC cells in vitro and in vivo in immunodeficient mice. METHODS:We utilized RNA interference to knockdown Slug gene expr...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Tang P,Yu Z,Zhang K,Wang Y,Ma Z,Zhang S,Chen D,Zhou Y

    更新日期:2011-05-20 00:00:00

  • The addition of locust bean gum but not water delayed the gastric emptying rate of a nutrient semisolid meal in healthy subjects.

    abstract:BACKGROUND:Most of the previous studies regarding the effects of gel-forming fibres have considered the gastric emptying of liquid or solid meals after the addition of pectin or guar gum. The influence of locust bean gum, on gastric emptying of nutrient semisolid meals in humans has been less well studied, despite its ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Darwiche G,Björgell O,Almér LO

    更新日期:2003-06-06 00:00:00

  • Asymptomatic hepatic artery dissection early after living-donor liver transplantation with simultaneous splenectomy: two case reports.

    abstract:BACKGROUND:Hepatic artery dissection after liver transplantation is an uncommon morbidity. The onset mechanism and management for this disorder remain unclear. The present report describes the cases of two patients with hepatic artery dissection after living-donor liver transplantation (LDLT) with simultaneous splenect...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Shimata K,Sugawara Y,Irie T,Sambommatsu Y,Kadohisa M,Ibuki S,Kawabata S,Isono K,Honda M,Yamamoto H,Hibi T

    更新日期:2020-11-12 00:00:00

  • Haemolytic uraemic syndrome - a rare case report of bloody diarrhoea in adults.

    abstract:BACKGROUND:Haemolytic uraemic syndrome is a rarely seen in adults often leading to critical illness. This case highlights how difficult it can be to establish a diagnosis and treat when a patient presents with bloody diarrhoea. CASE PRESENTATION:A 17-year-old Iraqi man presented to the emergency department with abdomi...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Radhakrishnan ST,Ruban A,Uthayakumar AK,Cohen P,Levy J,Teare J

    更新日期:2019-08-28 00:00:00

  • Positive regulatory effects of perioperative probiotic treatment on postoperative liver complications after colorectal liver metastases surgery: a double-center and double-blind randomized clinical trial.

    abstract:BACKGROUND:Colorectal liver metastases (CLM) occur frequently and postoperative intestinal infection is a common complication. Our previous study showed that probiotics could decrease the rate of infectious complications after colectomy for colorectal cancer. To determine the effects of the perioperative administration...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Liu Z,Li C,Huang M,Tong C,Zhang X,Wang L,Peng H,Lan P,Zhang P,Huang N,Peng J,Wu X,Luo Y,Qin H,Kang L,Wang J

    更新日期:2015-03-20 00:00:00

  • Effect of antecolic or retrocolic reconstruction of the gastro/duodenojejunostomy on delayed gastric emptying after pancreaticoduodenectomy: a meta-analysis.

    abstract:BACKGROUND:Delayed gastric emptying (DGE) is one of the most frequent complications after pancreaticoduodenectomy (PD). This meta-analysis aimed to evaluate the effect of antecolic versus retrocolic reconstruction of gastro/duodenojejunostomy on DGE after PD. METHODS:Randomized controlled trials (RCTs) comparing antec...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,meta分析


    authors: Zhou Y,Lin J,Wu L,Li B,Li H

    更新日期:2015-06-16 00:00:00

  • Acetic acid-indigo carmine chromoendoscopy for delineating early gastric cancers: its usefulness according to histological type.

    abstract:BACKGROUND:Endoscopic treatments, such as endoscopic submucosal dissection (ESD) and laparoscopic gastrectomy, are increasingly used to treat a subset of patients with early gastric cancer (EGC). To achieve successful outcomes, it is very important to accurately determine the lateral extent of the tumor. Therefore, we ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Lee BE,Kim GH,Park DY,Kim DH,Jeon TY,Park SB,You HS,Ryu DY,Kim DU,Song GA

    更新日期:2010-08-23 00:00:00

  • Gall bladder wall thickening as non-invasive screening parameter for esophageal varices - a comparative endoscopic - sonographic study.

    abstract:BACKGROUND:The mortality due to hemorrhage of esophageal varices (EV) is still high. The predominant cause for EV is liver cirrhosis, which has a high prevalence in Western Europe. Therefore, non-invasive screening markers for the presence of EV are of interest. Here, we aim to investigate whether non-inflammatory gall...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Tsaknakis B,Masri R,Amanzada A,Petzold G,Ellenrieder V,Neesse A,Kunsch S

    更新日期:2018-08-02 00:00:00

  • Conservative recovery and replacement of a ruptured percutaneous endoscopic gastrostomy tube; a case report.

    abstract:BACKGROUND:Percutaneous Endoscopic Gastrostomy (PEG) feeding tubes are frequently placed in patients to provide enteral nutrition. We report a case of a complete rupture of a PEG tube intra-abdominally with associated peritonitis after more than a month of PEG placement and utilization. To our knowledge, this is a very...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Al Halabi M,Wakim W,Moukaddam H,Husari A

    更新日期:2020-03-30 00:00:00

  • Plastic or metal stents for benign extrahepatic biliary strictures: a systematic review.

    abstract:BACKGROUND:Benign biliary strictures may be a consequence of surgical procedures, chronic pancreatitis or iatrogenic injuries to the ampulla. Stents are increasingly being used for this indication, however it is not completely clear which stent type should be preferred. METHODS:A systematic review on stent placement f...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,评审


    authors: van Boeckel PG,Vleggaar FP,Siersema PD

    更新日期:2009-12-17 00:00:00

  • Korean colonoscopy screening pilot study (K-cospi) for screening colorectal cancer: study protocol for the multicenter, community-based clinical trial.

    abstract:BACKGROUND:The Korean National Cancer Screening Program has been providing annual fecal immunochemical test for colorectal cancer (CRC) to adults aged 50 years and older since 2004. The Korean Colonoscopy Screening Pilot Study was developed to evaluate the effectiveness of colonoscopy screening for CRC incidence and mo...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Park B,Jun JK,Kim BC,Choi KS,Suh M,Expert Advisory Committee.,Monitoring Committee.,Center for Korean Colonoscopy Screening Pilot Study.,Research Team on the Protocol Development of Pilot study.

    更新日期:2021-01-26 00:00:00

  • Narrow-band imaging and high-definition white-light endoscopy in patients with serrated lesions not fulfilling criteria for serrated polyposis syndrome: a randomized controlled trial with tandem colonoscopy.

    abstract:BACKGROUND:It is unknown whether narrow-band imaging (NBI) could be more effective than high-definition white-light endoscopy (HD-WLE) in detecting serrated lesions in patients with prior serrated lesions > 5 mm not completely fulfilling serrated polyposis syndrome (SPS) criteria. METHODS:We conducted a randomized, cr...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,随机对照试验


    authors: Riu Pons F,Andreu M,Naranjo D,Álvarez-González MA,Seoane A,Dedeu JM,Barranco L,Bessa X

    更新日期:2020-04-16 00:00:00

  • Low bone mass in microscopic colitis.

    abstract:BACKGROUND:Microscopic colitis presents with similar symptoms to classic inflammatory bowel diseases. Osteoporosis is a common complication of Crohn's disease but there are no data concerning bone metabolism in microscopic colitis. AIMS:The aim of the present study was to evaluate bone density and metabolism in patien...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Lorinczy K,Lakatos G,Müllner K,Hritz I,Lakatos PL,Tulassay Z,Miheller P

    更新日期:2011-05-19 00:00:00

  • Abdominal migraine in children: association between gastric motility parameters and clinical characteristics.

    abstract:BACKGROUND:Approximately 0.2-1 % of children suffers from abdominal migraine (AM). Pathophysiology of AM has not been adequately studied. This study evaluated gastric motility in children with AM. METHODS:Seventeen children (6 boys), within an age range of 4-15 years, referred to a tertiary care paediatric unit, North...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Devanarayana NM,Rajindrajith S,Benninga MA

    更新日期:2016-02-29 00:00:00

  • Comparability of localization data in transnasal and transoral esophagogastroduodenoscopy.

    abstract:BACKGROUND:Esophagogastroduodenoscopy is an often-used and safe diagnostic method in gastroenterology. Transnasal esophagogastroduodenoscopy is now an established addition to the endoscopic instrumentarium. Although the two examination methods can be used alongside each other, there is a lack of studies on the comparab...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Aymaz S,Krakamp B,Kirschberg O,Lefering R

    更新日期:2010-10-13 00:00:00

  • Helicobacter pylori infection progresses proximally associated with pyloric metaplasia in age-dependent tendency: a cross-sectional study.

    abstract:BACKGROUND:The elderly population presents higher morbidity of H. pylori associated diseases in proximal stomach. The specific pathogenesis and mechanism have not been clearly addressed. The gastric environment for H. pylori colonization is dynamic with increasing age. The aim of present study is to investigate the cor...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Shi H,Xiong H,Qian W,Lin R

    更新日期:2018-10-29 00:00:00

  • Child-Pugh classification dependent alterations in serum leptin levels among cirrhotic patients: a case controlled study.

    abstract:BACKGROUND:As anorexia and hypermetabolism are common in cirrhosis, leptin levels may be increased in this disease. In this study, we investigated the relation between the severity of disease and serum leptin levels in post-hepatitis cirrhosis and the role of body composition, gender and viral aetiology of cirrhosis in...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,多中心研究


    authors: Bolukbas FF,Bolukbas C,Horoz M,Gumus M,Erdogan M,Zeyrek F,Yayla A,Ovunc O

    更新日期:2004-09-23 00:00:00