Abstract:
BACKGROUND:Pancreatic cancer has significant morbidity and mortality worldwide. Good prognosis relies on an early diagnosis. The purpose of this study was to develop techniques for identifying cancer biomarkers in the serum of patients with pancreatic cancer. METHODS:Serum samples from five individuals with pancreatic cancer and five individuals without cancer were compared. Highly abundant serum proteins were depleted by immuno-affinity column. Differential protein analysis was performed using 2-dimensional differential in-gel electrophoresis (2D-DIGE). RESULTS:Among these protein spots, we found that 16 protein spots were differently expressed between the two mixtures; 8 of these were up-regulated and 8 were down-regulated in cancer. Mass spectrometry and database searching allowed the identification of the proteins corresponding to the gel spots. Up-regulation of mannose-binding lectin 2 and myosin light chain kinase 2, which have not previously been implicated in pancreatic cancer, were observed. In an independent series of serum samples from 16 patients with pancreatic cancer and 16 non-cancer-bearing controls, increased levels of mannose-binding lectin 2 and myosin light chain kinase 2 were confirmed by western blot. CONCLUSIONS:These results suggest that affinity column enrichment and DIGE can be used to identify proteins differentially expressed in serum from pancreatic cancer patients. These two proteins 'mannose-binding lectin 2 and myosin light chain kinase 2' might be potential biomarkers for the diagnosis of the pancreatic cancer.
journal_name
BMC Gastroenteroljournal_title
BMC gastroenterologyauthors
Rong Y,Jin D,Hou C,Hu J,Wu W,Ni X,Wang D,Lou Wdoi
10.1186/1471-230X-10-68subject
Has Abstractpub_date
2010-06-29 00:00:00pages
68issn
1471-230Xpii
1471-230X-10-68journal_volume
10pub_type
杂志文章abstract:BACKGROUND:Reactive lymphoid hyperplasia (RLH) of the liver is very rarely reported, and we encountered two cases of RLH of the liver in a patient with colon cancer. CASE PRESENTATION:In the first case, a 77-year-old woman was admitted for the surgical removal of a ascending colon cancer. A hepatic tumor in the left l...
journal_title:BMC gastroenterology
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doi:10.1186/1471-230X-6-25
更新日期:2006-09-12 00:00:00
abstract:BACKGROUND:Pegylated interferon (PEG-IFN) alfa-2b is recommended for chronic hepatitis B (CHB). We aimed to investigate the sustainability of off-treatment responses among Chinese HBeAg-positive CHB patients treated with PEG-IFN alfa-2b from a randomized trial. METHODS:Eligible Chinese patients (n = 322) were followed...
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pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2012-10-15 00:00:00
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pub_type: 杂志文章
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journal_title:BMC gastroenterology
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doi:10.1186/s12876-018-0744-8
更新日期:2018-01-26 00:00:00
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journal_title:BMC gastroenterology
pub_type: 杂志文章
doi:10.1186/1471-230X-14-74
更新日期:2014-04-11 00:00:00
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pub_type: 杂志文章
doi:10.1186/1471-230X-13-66
更新日期:2013-04-15 00:00:00
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pub_type: 杂志文章
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更新日期:2003-06-06 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12876-020-01528-0
更新日期:2020-11-12 00:00:00
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pub_type: 杂志文章
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更新日期:2019-08-28 00:00:00
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pub_type: 杂志文章,多中心研究,随机对照试验
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pub_type: 杂志文章,meta分析
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更新日期:2010-08-23 00:00:00
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更新日期:2018-08-02 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12876-020-01218-x
更新日期:2020-03-30 00:00:00
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journal_title:BMC gastroenterology
pub_type: 杂志文章,评审
doi:10.1186/1471-230X-9-96
更新日期:2009-12-17 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12876-021-01610-1
更新日期:2021-01-26 00:00:00
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pub_type: 杂志文章,随机对照试验
doi:10.1186/s12876-020-01257-4
更新日期:2020-04-16 00:00:00
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pub_type: 杂志文章
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更新日期:2011-05-19 00:00:00
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pub_type: 杂志文章
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更新日期:2016-02-29 00:00:00
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pub_type: 杂志文章
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更新日期:2010-10-13 00:00:00
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journal_title:BMC gastroenterology
pub_type: 杂志文章
doi:10.1186/s12876-018-0883-y
更新日期:2018-10-29 00:00:00
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pub_type: 杂志文章,多中心研究
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更新日期:2004-09-23 00:00:00