Abstract:
:Damage of non-vascularised tissues such as cartilage and cornea can result in healing processes accompanied by a non-physiological angiogenesis. Peptidic aptamers have recently been reported to block the vascular endothelial growth factor (VEGF). However, the therapeutic applications of these aptamers are limited due to their short half-life in vivo. In this work, an enhanced stability and bioavailability of a known VEGF blocker aptamer sequence (WHLPFKC) was pursued through its tethering of molecular scaffolds based on hyperbranched peptides, the poly(ɛ-lysine) dendrons, bearing three branching generations. The proposed design allowed simultaneous and orderly-spaced exposure of 16 aptamers per dendrimer to the surrounding biological microenvironent, as well as a relatively hydrophobic core based on di-phenylalanine aiming to promote an hydrophobic interaction with the hydrophobic moieties of ionically crosslinked methacrylated gellan gum (iGG-MA) hydrogels. The VEGF blocker dendrons were entrapped in iGG-MA hydrogels, and their capacity to prevent endothelial cell sprouting was assessed qualitatively and quantitatively using 3D in vitro models and the in vivo chick chorioallantoic membrane assay. The data demonstrate that at nanoscale concentrations, the dendronised structures were able to enhance control of the biological actvity of WHLPFKC at the material/tissue interface and hence the anti-angiogenic capacity of iGG-MA hydrogels not only preventing blood vessel invasion, but also inducing their regression at the tissue/iGG-MA interface. The in ovo study confirmed that iGG-MA functionalised with the dendron VEGF blockers do inhibit angiogenesis by controlling both size and ramifications of blood vessels in the proximity of the implanted gel surface. Copyright © 2016 John Wiley & Sons, Ltd.
journal_name
J Tissue Eng Regen Medjournal_title
Journal of tissue engineering and regenerative medicineauthors
Perugini V,Guildford AL,Silva-Correia J,Oliveira JM,Meikle ST,Reis RL,Santin Mdoi
10.1002/term.2340subject
Has Abstractpub_date
2018-02-01 00:00:00pages
e669-e678issue
2eissn
1932-6254issn
1932-7005journal_volume
12pub_type
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2016-02-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2010-10-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.295
更新日期:2011-02-01 00:00:00
abstract::Tissue engineering applications require an appropriate combination of a cell population, biochemical factors and scaffold materials. In this field, mesenchymal stem cells (MSCs) emerge as an attractive cell population, due to their ready availability and their potential to be differentiated into various mesodermal cel...
journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2013-07-01 00:00:00
abstract::Silk fibroin (SF) hydrogels can be obtained via self-assembly, but this process takes several days or weeks, being unfeasible to produce cell carrier hydrogels. In this work, a phospholipid, namely, 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol) sodium salt (DMPG), was used to induce and accelerate the gelatio...
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journal_title:Journal of tissue engineering and regenerative medicine
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