Abstract：
OBJECTIVE:The present study aims to elucidate the role of Rho-mediated ROCK-Semaphorin3A signaling pathway in the pathogenesis of Parkinson's disease (PD) in a mouse model. METHODS:One-hundred twelve eight-week male C57BL/6 mice were selected. The mouse model of PD was constructed by intraperitoneal injection of MPTP. All mice were divided into four groups (28 mice in each group): Blank group, Model group, Rho knockout (Rho+/-) group and ROCK knockout (ROCK+/-) group. Changes of behavior of the mice were studied through automatic moving test and rotarod test. Immunohistochemistry (IHC) was used to detect the expressions of TH, CD11b and GFAP. High performance liquid chromatograph (HPLC) was performed for detection of dopamine and its metabolic product. The mRNA and protein expressions of Rho, ROCK, Sema3A, PlexinA and NRP-1 were detected using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. RESULTS:Rho and ROCK knockout improved the damage caused by MPTP on the behavior of mice and protected dopaminergic neurons from injury, along with the increases of dopamine and its metabolic product. The mRNA and protein expressions of Rho, ROCK, Sema3A, PlexinA and NRP-1 were increased in PD mice in the Model group compared with those in the Blank group. Compared to the Model group, the mRNA and protein expressions of Rho, ROCK, Sema3A, PlexinA and NRP-1 were reduced in the Rho+/- and ROCK+/- groups. CONCLUSION:These findings indicate that Rho and ROCK knockout may improve the behavior of mice and prevent MPTP-induced dopaminergic neurons damage by regulating Sema3A, PlexinA and NRP-1 in a mouse model of PD.
journal_name
J Neurol Scijournal_title
Journal of the neurological sciencesauthors
Qi L,Tang YG,Wang L,He W,Pan HH,Nie RR,Can Ydoi
10.1016/j.jns.2016.08.061subject
Has Abstractpub_date
2016-11-15 00:00:00pages
21-26eissn
0022-510Xissn
1878-5883pii
S0022-510X(16)30552-4journal_volume
370pub_type
杂志文章abstract：OBJECTIVE:The aim of the present systematic review is to critically evaluate the effectiveness of OMT as an adjuvant therapy in the management of patients with neurological diseases. METHODS:A systematic review was conducted and the findings were reported following the PRISMA statement. Twelve databases were searched ...
journal_title：Journal of the neurological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.jns.2016.08.062
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abstract：:Proinflammatory cytokines contribute to the regulation of the disease process in inflammatory neuropathies. Cellular localisation of cytokine expression in CIDP nerve biopsies should provide further insight into the pathogenic mechanisms of the disease and the individual cells involved. In this study in situ hybridisa...
journal_title：Journal of the neurological sciences
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doi：10.1016/s0022-510x(99)00009-x
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journal_title：Journal of the neurological sciences
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journal_title：Journal of the neurological sciences
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abstract：OBJECT:The aim of this study is to identify pre-operative clinical and/or radiological predictors of clinical failure of decompressive hemicraniectomy (DH) in the setting of malignant hemispheric infarction. These predictors could guide the decision for adjunctive internal brain decompression (e.g. strokectomy) at the ...
journal_title：Journal of the neurological sciences
pub_type： 杂志文章
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journal_title：Journal of the neurological sciences
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journal_title：Journal of the neurological sciences
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journal_title：Journal of the neurological sciences
pub_type： 杂志文章
doi：10.1016/j.jns.2013.11.019
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journal_title：Journal of the neurological sciences
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doi：10.1016/j.jns.2013.03.021
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更新日期：1984-01-01 00:00:00
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journal_title：Journal of the neurological sciences
pub_type： 杂志文章
doi：10.1016/j.jns.2014.12.043
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journal_title：Journal of the neurological sciences
pub_type： 杂志文章,meta分析
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pub_type： 杂志文章,评审
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更新日期：2000-04-01 00:00:00
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journal_title：Journal of the neurological sciences
pub_type： 杂志文章
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journal_title：Journal of the neurological sciences
pub_type： 杂志文章
doi：
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更新日期：1979-08-01 00:00:00
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pub_type： 杂志文章,随机对照试验
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更新日期：2009-07-15 00:00:00
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更新日期：2011-05-15 00:00:00
