Abstract:
BACKGROUND:A functional cure of chronic hepatitis B (CHB) is feasible, but a clear view of the intrahepatic viral dynamics in each patient is needed. Intrahepatic covalently closed circular DNA (cccDNA) is the stable form of the viral genome in infected cells, and represents the ideal marker of parenchymal colonization. Its relationships with easily accessible peripheral parameters need to be elucidated in order to avoid invasive procedures in patients. OBJECTIVES:The goal of this study was to design, set up, and validate a reliable and straightforward method for the quantification of the cccDNA and total DNA of the hepatitis B virus (HBV) in a variety of clinical samples. PATIENTS AND METHODS:Clinical samples from a cohort of CHB patients, including liver biopsies in some, were collected for the analysis of intracellular HBV molecular markers using novel molecular assays. RESULTS:A plasmid construct, including sequences from the HBV genome and from the human gene hTERT, was generated as an isomolar multi-standard for HBV quantitation and normalization to the cellular contents. The specificity of the real-time assay for the cccDNA was assessed using Dane particles isolated on a density gradient. A comparison of liver tissue from 6 untreated and 6 treated patients showed that the treatment deeply reduced the replicative capacity (total DNA/cccDNA), but had limited impact on the parenchymal colonization. The peripheral blood mononuclear cells (PBMCs) and granulocytes from the treated and untreated patients were also analyzed. CONCLUSIONS:A straightforward method for the quantification of intracellular HBV molecular parameters in clinical samples was developed and validated. The widespread use of such versatile assays could better define the prognosis of CHB, and allow a more rational approach to time-limited tailored treatment strategies.
journal_name
Hepat Monjournal_title
Hepatitis monthlyauthors
Minosse C,Coen S,Visco Comandini U,Lionetti R,Montalbano M,Cerilli S,Vincenti D,Baiocchini A,Capobianchi MR,Menzo Sdoi
10.5812/hepatmon.28751subject
Has Abstractpub_date
2016-02-29 00:00:00pages
e28751issue
10eissn
1735-143Xissn
1735-3408journal_volume
16pub_type
杂志文章abstract:UNLABELLED:CONTEXTS: Hepatocellular carcinoma (HCC) is one of the most common malignant diseases in the world. Because less than 20% of patients with HCC are resectable, various types of non-surgical treatment have been developed. EVIDENCE ACQUISITION:At present, radiofrequency ablation (RFA) is accepted as the standa...
journal_title:Hepatitis monthly
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pub_type: 杂志文章,评审
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abstract:BACKGROUND:Reversibility of advanced fibrosis after HCV-clearance is an important goal of therapy. OBJECTIVES:Measuring liver stiffness (LS) by transient elastography (TE) might be helpful in this setting. PATIENTS AND METHODS:We evaluated 104 patients with biopsy-proven chronic hepatitis C (CHC) and sustained virolo...
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abstract:BACKGROUND:There have been very few studies evaluating the close association between excess iron and cirrhosis; however, cirrhosis could be regarded as an iron-loading disorder. OBJECTIVES:In this study, the goal was to show the levels of the iron content in the liver tissue in certain types of cirrhosis. PATIENTS AN...
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abstract:BACKGROUND AND AIMS:The duration of the protection of hepatitis B vaccination for infants and the necessity of a booster dose administration is unknown. The aim of the present study was to evaluate the persistence of seroprotection after hepatitis B virus (HBV) vaccination in order to determine the necessity of a singl...
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abstract:BACKGROUND:Viral hepatitis is a major health problem in Brazil and worldwide. The state of Santa Catarina has high endemic areas, and the disease burden is unknown. OBJECTIVES:To estimate and analyze the burden of hepatitis B in the state of Santa Catarina, in 2005 - 2010. MATERIALS AND METHODS:A time-series analysis...
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abstract:BACKGROUND:There are several studies in the literature investigating factors which can induce non-alcoholic fatty liver disease (NAFLD) in different populations. However, the existing literature lacks powerful studies addressing the factors which may predict the severity of NAFLD. OBJECTIVES:In the current study, we a...
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journal_title:Hepatitis monthly
pub_type: 杂志文章
doi:
更新日期:2011-07-01 00:00:00
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更新日期:2011-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-05-23 00:00:00
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doi:
更新日期:2011-04-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2014-03-01 00:00:00
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doi:
更新日期:2011-06-01 00:00:00
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更新日期:2015-03-05 00:00:00
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更新日期:2013-10-01 00:00:00
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更新日期:2016-03-05 00:00:00
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pub_type: 杂志文章
doi:10.5812/hepatmon.31490
更新日期:2015-10-28 00:00:00
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更新日期:2015-09-29 00:00:00