当前位置： SCI文献检索 > Hepatitis Monthly期刊下所有文献 > Serological Patterns and Molecular Characterization of Occult Hepatitis B Virus Infection among Blood Donors.

Serological Patterns and Molecular Characterization of Occult Hepatitis B Virus Infection among Blood Donors.

Abstract：

BACKGROUND:Hepatitis B infections, characterized by the presence of a viral genome without detectable hepatitis B surface antigen (HBsAg; Occult hepatitis B infection [OBI]), have been reported recently. OBJECTIVES:We performed serological and molecular characterization of OBI among blood donors at Jiangsu province blood center during years 2013 and 2014. METHODS:All donor samples were routinely screened by double enzyme-linked immunosorbent assay (ELISA) for hepatitis C virus (HCV), hepatitis B virus (HBV), human immunodeficiency virus (HIV), Treponema pallidum (TP), and alanine aminotransferase (ALT). Single-reactive, nonreactive, and ALT-elevated samples were pooled or resolved by nucleic acid testing (NAT). Seromarkers were examined in HBsAg-/DNA+ samples. After 1 to 12 months of follow up, seromarkers were screened again to verify OBI samples. RESULTS:We studied 157119 samples from blood donors. A total of 154397 ELISA nonreactive donor samples were identified, and HBV DNA was detected in 81 samples; no samples were positive for HIV or HCV RNA. Hepatitis B virus viral loads in most donors were less than 20 - 200 IU/mL. There was only one HBsAg-positive sample. Eighty HBsAg-/DNA+ samples were evaluated further. Of these samples, 85% (68/80) were reactive for anti-HBc and 36.2% (29/800) were reactive for anti-HBc and anti-HBs; 11.3% (9/80) did not have any detectable serological markers. Twenty-nine donors were followed up. One was HBsAg ELISA positive, and of six seronegative donors, all had anti-HBc and anti-HBs, but were negative for DNA. Samples were HBV genotypes B, C and D. Mutations in the S region of HBV DNA included S114T, G119R, P120S, T125M, C139Y, T140I, C147W, T148A, A159V/G, E164D, V168A, and R169C. CONCLUSIONS:Overall, we found that OBI was rare, but that the prevalence of OBI was slightly higher in Jiangsu than in other areas of China.

journal_name

Hepat Mon

journal_title

Hepatitis monthly

authors

Lin H,Zhao H,Tang X,Hu W,Jiang N,Zhu S,Huang C

doi

10.5812/hepatmon.40492

subject

Has Abstract

pub_date

2016-10-02 00:00:00

pages

e40492

issue

eissn

1735-143X

issn

1735-3408

journal_volume

pub_type

杂志文章

在线工具

Serological Patterns and Molecular Characterization of Occult Hepatitis B Virus Infection among Blood Donors.