Preanalytics in urinalysis.

Abstract:

:Urine contains an enormous amount of information. Well-standardized procedures for collection, transport, sample preparation and analysis should become the basis of an effective diagnostic strategy for urinalysis. As reproducibility of urinalysis has been greatly improved due to recent technological progress, preanalytical requirements of urinalysis have gained importance and have become stricter. Since the patients themselves often collect urine specimens, urinalysis is very susceptible to preanalytical issues. Various collection methods and inappropriate specimen transport can cause important preanalytical errors. In addition to the insurance of correct collection, the clinical laboratory should optimize transport and sample preservation. Errors due to variation in diuresis may be corrected by recalculating the results using dilution parameters (e.g. osmolality, creatinine, conductivity, urine density). Next to the use of a primary urine container, it is recommended to split the original urine sample into various smaller aliquots for morphological, microbiological and chemical analyses, decreasing the risk of contamination. The use of preservatives may be helpful for particular analytes. A universal urine preservative however does not exist. Preanalytical aspects are also of major importance for newer urinalysis applications (e.g. metabolomics).

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Delanghe JR,Speeckaert MM

doi

10.1016/j.clinbiochem.2016.10.016

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

1346-1350

issue

18

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(16)30462-3

journal_volume

49

pub_type

杂志文章,评审
  • Combining high-sensitivity cardiac troponin and B-type natriuretic peptide in the detection of inducible myocardial ischemia.

    abstract:BACKGROUND:Single biomarker approaches provide only moderate accuracy in the non-invasive detection of exercise-induced myocardial ischemia. We therefore assessed the combination of the two most promising single biomarkers: high-sensitivity cardiac troponin I (hs-cTnI) and B-type natriuretic peptide (BNP). METHODS:Con...

    journal_title:Clinical biochemistry

    pub_type: 临床试验,杂志文章

    doi:10.1016/j.clinbiochem.2017.10.014

    authors: Puelacher C,Wagener M,Honegger U,Assadian M,Schaerli N,Mueller D,Strebel I,Twerenbold R,Boeddinghaus J,Nestelberger T,Wildi K,Sabti Z,Sazgary L,Badertscher P,du Fay de Lavallaz J,Marbot S,Kaiser C,Wild D,Zellweger MJ

    更新日期:2018-02-01 00:00:00

  • The measurement of serum cholinesterase activities by an integration strategy with expanded linear ranges and negligible substrate-activation.

    abstract:OBJECTIVES:To measure serum cholinesterase (SCHE) with an integration strategy. DESIGN AND METHODS:At 54.0 micromol/L butyrylthiolcholine, SCHE initial rates were calculated with 50.0 micromol/L butyrylthiolcholine and maximal rates via an improved integrated method if substrate consumptions within 5.0 min were over 6...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2008.11.016

    authors: Liao F,Yang D,Tang J,Yang X,Liu B,Zhao Y,Zhao L,Liao H,Yu M

    更新日期:2009-06-01 00:00:00

  • How variable are hydroxyproline determinations made in different samples of the same liver?

    abstract:OBJECTIVES:The haphazard distribution of fibrous tissue can interfere with quantitative methods for evaluating hepatic fibrosis. Inter-sample variation may represent a crucial issue when hydroxyproline measurement is used to quantify fibrosis. A comparative study of the hydroxyproline levels in normal and fibrotic rats...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2006.08.002

    authors: Gomes AT,Bastos CG,Afonso CL,Medrado BF,Andrade ZA

    更新日期:2006-12-01 00:00:00

  • Cytokine promoter polymorphisms in Taiwanese patients with Graves' disease.

    abstract:OBJECTIVES:This study aimed to examine the association between promoter polymorphisms of Th1 and Th2 cytokine genes [interleukin-4 (IL-4 T-34C, A-81G, C-285T and T-589C), IL-6 (G-174C), IL-10 (A-592C and T-819C) and tumour necrosis factor-alpha (TNF-alpha G-238A and G-308A)] and Graves' disease (GD) in Taiwanese popula...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2006.11.009

    authors: Shiau MY,Huang CN,Yang TP,Hwang YC,Tsai KJ,Chi CJ,Chang YH

    更新日期:2007-02-01 00:00:00

  • Medical errors: impact on clinical laboratories and other critical areas.

    abstract::The Institute of Medicine (IOM) report (1999) stated that the prevalence of medical errors is high in today's health care system. Some specialties in health care are more risky than others. A varying blunder/error rate of 0.1-9.3% in clinical diagnostic laboratories has been reported in the literature. Many of these e...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.clinbiochem.2004.08.009

    authors: Kalra J

    更新日期:2004-12-01 00:00:00

  • Phospholipase A2 group XV activity during cardiopulmonary bypass surgery.

    abstract:OBJECTIVES:All patients who undergo cardiopulmonary bypass (CPB) experience some degree of ischemia reperfusion injury (IRI). Severe IRI-induced acute kidney injury (AKI) occurs in approximately 1-2% of patients undergoing CPB. Previous studies using activity-based protein profiling of urine identified group XV phospho...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2020.12.003

    authors: Navarrete M,Wilkins JA,Chan FV,Ye B,Nickerson P,Ho J

    更新日期:2021-02-01 00:00:00

  • An audit of holotranscobalamin ("Active" B12) and methylmalonic acid assays for the assessment of vitamin B12 status: application in a mixed patient population.

    abstract:BACKGROUND:Vitamin B12 insufficiency/deficiency is common in mixed patient populations. However there is no single marker which can reliably diagnose B12 insufficiency/deficiency. Elevated concentrations of methylmalonic acid (MMA) are considered the most representative marker of metabolic vitamin B12 insufficiency, bu...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2013.08.006

    authors: Sobczyńska-Malefora A,Gorska R,Pelisser M,Ruwona P,Witchlow B,Harrington DJ

    更新日期:2014-01-01 00:00:00

  • Relationship between serum butyrylcholinesterase and the metabolic syndrome.

    abstract:OBJECTIVE:To identify independent predictors of serum butyrylcholinesterase (BuChE) activity and examine its relationship with the metabolic syndrome. DESIGN AND METHODS:Serum BuChE activity was measured and metabolic syndrome risk factors were assessed in 1097 healthy subjects recruited from the Newfoundland adult po...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2005.04.008

    authors: Randell EW,Mathews MS,Zhang H,Seraj JS,Sun G

    更新日期:2005-09-01 00:00:00

  • Nonalcoholic steatohepatitis: recent advances from experimental models to clinical management.

    abstract::A condition defined as nonalcoholic fatty liver disease (NAFLD) is frequently found in humans. Deemed as a benign condition until recently, more emphasis is now put on the potential harmful evolution of the inflammatory form, that is, nonalcoholic steatohepatitis (NASH), toward end-stage liver disease. This review hig...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.clinbiochem.2004.10.014

    authors: Portincasa P,Grattagliano I,Palmieri VO,Palasciano G

    更新日期:2005-03-01 00:00:00

  • Does interferon and ribavirin combination therapy ameliorate growth hormone deficiency in HCV genotype-4 infected patients?

    abstract:OBJECTIVES:To explore the impact of response to interferon and ribavirin antiviral therapy on human growth hormone (hGH) levels in Egyptian chronic hepatitis C genotype-4 infected patients. DESIGN AND METHODS:We studied eighty Egyptian HCV infected patients visiting outpatient clinics of Tropical Medicine and Hepatolo...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2011.08.1145

    authors: Eskander EF,Abd-Rabou AA,Yahya SM,Shaker OG,Mohamed MS

    更新日期:2012-01-01 00:00:00

  • Genetic risk factors for renal failure among north Indian ESRD patients.

    abstract:OBJECTIVES:Angiotensin converting enzyme (ACE), G-Protein couple receptor (G-Prot), endothelial nitric oxide synthase (ecNOS), Leptin -2548G/A and uncoupling protein (UCP2) are potent regulators of intra renal hemodynamics and may be the causative factors contributing to the deterioration of renal functions. In recent ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2008.01.009

    authors: Tripathi G,Sharma RK,Baburaj VP,Sankhwar SN,Jafar T,Agrawal S

    更新日期:2008-05-01 00:00:00

  • Disinfectant wipes containing hydrogen peroxide induce overestimation of glucose results obtained with Lifescan SureStep Flexx® glucose meter.

    abstract:OBJECTIVES:Investigate the interference of Virox® disinfectant wipes containing hydrogen peroxide with Lifescan SureStep Flexx® glucose meter results. DESIGN AND METHODS:Glucose measurements of control and whole blood samples were performed before and after cleaning with Virox® wipes to reveal the kinetics of this int...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2010.09.012

    authors: Desmeules P,Ethier J,Allard P

    更新日期:2010-12-01 00:00:00

  • Metabolomic and bioinformatic analyses in asphyxiated neonates.

    abstract:OBJECTIVES:We tested the application of bioinformatic algorithms in studying the metabolomic profiles of neonatal urine samples with clinical evidence of severe asphyxia at birth and subsequent neurodevelopmental handicap. DESIGN AND METHODS:The clinical outcomes of 256 newborns that required direct admission to neona...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2006.01.006

    authors: Chu CY,Xiao X,Zhou XG,Lau TK,Rogers MS,Fok TF,Law LK,Pang CP,Wang CC

    更新日期:2006-03-01 00:00:00

  • Increased glutathionylated hemoglobin (HbSSG) in type 2 diabetes subjects with microangiopathy.

    abstract:OBJECTIVE:Protein glutathionylation is considered an important post-translational modification in the pathogenesis of complex diseases. The aim of this study was to examine whether hemoglobin (Hb) is modified by reduced glutathione (GSH) via oxidation of the thiol groups present in diabetes and its associated microangi...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2005.06.009

    authors: Sampathkumar R,Balasubramanyam M,Sudarslal S,Rema M,Mohan V,Balaram P

    更新日期:2005-10-01 00:00:00

  • Undetectable AMH at 3 days of age: a clue to bilateral anorchia.

    abstract::We report the case of a newborn boy who presented with micropenis and bilateral cryptorchidism at birth. The determination of AMH level strongly suggested a diagnosis of bilateral anorchia at 3 days of age whereas FSH and LH levels at the same time were not informative. The rapidity and reliability of results can redu...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2010.08.022

    authors: Stoppa-Vaucher S,Djemli A,Van Vliet G

    更新日期:2010-11-01 00:00:00

  • A retrospective analysis of mycophenolic acid and cyclosporin concentrations with acute rejection in renal transplant recipients.

    abstract:OBJECTIVES:Although monitoring of cyclosporin (CsA) is standard clinical practice postrenal transplantation, mycophenolic acid (MPA) concentrations are not routinely measured. There is evidence that a relationship exists between MPA area under the concentration-time curve (AUC) and rejection. In this study, a retrospec...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(00)00196-x

    authors: Pillans PI,Rigby RJ,Kubler P,Willis C,Salm P,Tett SE,Taylor PJ

    更新日期:2001-02-01 00:00:00

  • Haplotypes in the UGT1A1 gene and their role as genetic determinants of bilirubin concentration in healthy German volunteers.

    abstract:BACKGROUND:Genetic variations of UDP-glucuronyltransferase 1A1 (UGT1A1) influence the concentration of serum bilirubin. We investigated the association of four common polymorphisms including UGT1A1-53(TA)(n), and common haplotypes of the UGT1A1 gene with bilirubin levels in 218 Caucasian volunteers. METHODS:Total bili...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2009.08.011

    authors: Borucki K,Weikert C,Fisher E,Jakubiczka S,Luley C,Westphal S,Dierkes J

    更新日期:2009-11-01 00:00:00

  • A comparative study of three approaches to the routine quantitation of human serum proteins.

    abstract::The Laser Nephelometer PDQTM (Hyland Division, Travenol Laboratories Inc.) and the Abbott Bichromatic Analyser 100 (Abbott Laboratories) were compared with a radial immunodiffusion method. Seventy-eight serum specimens collected during routine blood testing were aliquoted and quantitated by the three procedures descri...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(78)90126-1

    authors: Bruver RM,Salkie ML

    更新日期:1978-06-01 00:00:00

  • Comparative evaluation of the chemopreventive efficacy of green and black tea polyphenols in the hamster buccal pouch carcinogenesis model.

    abstract:OBJECTIVES:To evaluate the comparative chemopreventive efficacy of green tea polyphenols (Polyphenon-E) and black tea polyphenols (Polyphenon-B) on 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis. DESIGN AND METHODS:Hamsters were divided into 6 groups. Animals in group 1 served ...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2005.06.011

    authors: Chandra Mohan KV,Hara Y,Abraham SK,Nagini S

    更新日期:2005-10-01 00:00:00

  • Performance characteristics of six automated 25-hydroxyvitamin D assays: Mind your 3s and 2s.

    abstract:BACKGROUND:Measurement of 25(OH)D has evolved rapidly, with an increased number of high-throughput automated immunoassays becoming available in recent years. The aim of this study was to fully evaluate six commercially available automated 25(OH)D immunoassays, including 2 newly available (Beckman Coulter) and one recal...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2015.08.005

    authors: Wyness SP,Straseski JA

    更新日期:2015-11-01 00:00:00

  • Study of FibroTest and hyaluronic acid biological variation in healthy volunteers and comparison of serum hyaluronic acid biological variation between chronic liver diseases of different etiology and fibrotic stage using confidence intervals.

    abstract:OBJECTIVES:Personalized ranges of liver fibrosis serum biomarkers such as FibroTest or hyaluronic acid could be used for early detection of fibrotic changes in patients with progressive chronic liver disease. Our aim was to generate reliable biological variation estimates for these two biomarkers with confidence interv...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2015.03.020

    authors: Istaces N,Gulbis B

    更新日期:2015-07-01 00:00:00

  • Evaluation of Elecsys free triiodothyronine assay: relevance of age-related reference ranges.

    abstract:OBJECTIVE:This study aimed at evaluating Elecsys free triiodothyronine (FT3) assay performed on an Elecsys 2010 system, while paying special attention to age relationship in euthyroid subjects. DESIGN AND METHODS:FT3 levels were measured in 149 euthyroid control subjects aged between 2 and 92 years old, 33 hyperthyroi...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0009-9120(98)00024-1

    authors: Sapin R,Schlienger JL,Goichot B,Gasser F,Grucker D

    更新日期:1998-07-01 00:00:00

  • Expression analysis and clinical utility of L-Dopa decarboxylase (DDC) in prostate cancer.

    abstract:BACKGROUND:L-Dopa decarboxylase (DDC) is a pyridoxal 5'-phosphate-dependent enzyme that was found to be involved in many malignancies. The aim of this study was to investigate the mRNA expression levels of DDC in prostate tissues and to evaluate its clinical utility in prostate cancer (CaP). METHODS:Total RNA was isol...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2008.04.026

    authors: Avgeris M,Koutalellis G,Fragoulis EG,Scorilas A

    更新日期:2008-10-01 00:00:00

  • The thyroid stimulating hormone receptor in human disease.

    abstract::The initial step in the action of thyrotropin (TSH) is its binding to the TSH receptor. TSH receptor antibodies are detected in up to 90% of patients with Graves' disease. Serial measurements of TSH receptor antibodies in patients with Graves' hyperthyroidism are helpful in predicting relapse. The TSH receptor was pur...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/0009-9120(90)90417-s

    authors: Harrison LC,Leedman PJ

    更新日期:1990-02-01 00:00:00

  • Soluble serum Klotho in diabetic nephropathy: relationship to VEGF-A.

    abstract:OBJECTIVES:Both kidney expression and soluble serum Klotho are influenced by chronic kidney disease (CKD) and diabetes. Serum Klotho is a yet poorly explored biomarker. We describe, for the first time to our knowledge, serum Klotho in diabetic patients with CKD and its relationship to vascular endothelial growth factor...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2012.07.098

    authors: Kacso IM,Bondor CI,Kacso G

    更新日期:2012-11-01 00:00:00

  • Circulating miRNA as fine regulators of the physiological responses to physical activity: Pre-analytical warnings for a novel class of biomarkers.

    abstract::MicroRNAs are endogenous non-coding RNAs that post-transcriptionally regulate gene expression by specifically binding the target mRNA and by consequently inducing its degradation. miRNAs can be released into the circulation where they remain stable and they can be measured. Their changes reflect individual biologic ad...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.clinbiochem.2016.09.017

    authors: Lombardi G,Perego S,Sansoni V,Banfi G

    更新日期:2016-12-01 00:00:00

  • Pediatric reference values for serum zinc concentration in Iranian subjects and an assessment of their dietary zinc intakes.

    abstract:OBJECTIVES:To determine pediatric reference values for serum zinc concentration in Iranian subjects. DESIGN AND METHODS:Serum zinc concentration was measured by flame atomic absorption spectrometry in 699 children and adolescents. Reference values for serum zinc were determined according to the Clinical and Laboratory...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2012.07.086

    authors: Ghasemi A,Zahediasl S,Hosseini-Esfahani F,Syedmoradi L,Azizi F

    更新日期:2012-10-01 00:00:00

  • Characterization of a novel isolated deletion of the exon 3 within the CFTR gene: Relevance for phenotypic expression and genetic counseling.

    abstract:OBJECTIVES:To characterize a novel deletion of exon 3 of CFTR gene and to evaluate the implications in Cystic Fibrosis (CF) care and genetic counseling. DESIGN AND METHODS:We performed a wide mutational analysis of CFTR gene, using reverse dot blot, Multiplex Ligation-dependent Probe Amplification (MLPA) assay and Rea...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2011.03.140

    authors: Tomaiuolo AC,Sirleto P,Centrone C,Surace C,Alghisi F,Petrocchi S,Lombardo A,Rossi M,Torricelli F,Lucidi V,Angioni A

    更新日期:2011-07-01 00:00:00

  • Proportion of conjugated bilirubin in bile in relation to hepatic bilirubin UDP-glucuronyltransferase activity.

    abstract::To elucidate the diagnostic relevance of biliary conjugated bilirubin, biliary bilirubin from normal volunteers (NV), patients with Gilbert's syndrome (GS) and Crigler-Najjar syndrome type II (C-N II), and from various rat strains was fractionated. Biliary bilirubin diglucuronide (BDG) was present at lower levels, and...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/0009-9120(90)80024-d

    authors: Adachi Y,Yamashita M,Nanno T,Yamamoto T

    更新日期:1990-04-01 00:00:00

  • The TCN2 776CNG polymorphism correlates with vitamin B(12) cellular delivery in healthy adult populations.

    abstract:OBJECTIVES:Vitamin B(12), or B(12), is an essential nutrient for humans, and its deficiency is a public health problem, especially in elderly population. Around 30% of circulating total B(12) levels are attached to transcobalamin II (TCN2), being referred as holotranscobalamin (holo-TC), and representing the biological...

    journal_title:Clinical biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.clinbiochem.2010.01.015

    authors: Castro R,Barroso M,Rocha M,Esse R,Ramos R,Ravasco P,Rivera I,de Almeida IT

    更新日期:2010-05-01 00:00:00