Abstract：
:Dexmedetomidine, an agonist of alpha2-adrenergic receptors, is used for critically ill patients to induce and maintain sedation and analgesia. Brain ischemia/reperfusion (I/R) usually causes severe neuronal injuries to intensive care unit patients. This study was aimed to evaluate the effects of dexmedetomidine on I/R-induced insults to neuronal cells and the possible mechanisms. Treatment of neuro-2a cells with dexmedetomidine did not affect cell viability but could protect against I/R-induced cell death. Separately, the I/R-triggered cell shrinkage, DNA fragmentation, and apoptosis in neuro-2a cells were alleviated by dexmedetomidine. As to the mechanisms, exposure of neuro-2a cells to dexmedetomidine substantially attenuated I/R-induced translocation of Bax protein from the cytosol to mitochondria and reduction in the mitochondrial membrane potential (MMP). Successively, dexmedetomidine decreased cytochrome c release from mitochondria to the cytoplasm and consequent cascade activations of caspases-9, -3, and -6 in I/R-treated neuro-2a cells. Interestingly, downregulating caspase-6 activity synergistically improved dexmedetomidine-induced defense against I/R-induced apoptosis of neuro-2a cells. The dexmedetomidine-involved neuroprotection was further confirmed in the other NB41A3 neuronal cells by significantly attenuating I/R-induced changes in the MMP, caspase-3 activation, DNA fragmentation, and cell apoptosis. Taken together, this study has shown the neuroprotective effects of dexmedetomidine against I/R-induced apoptotic insults via an intrinsic Bax-mitochondria-cytochrome c-caspase protease pathway. J. Cell. Biochem. 118: 2635-2644, 2017. © 2016 Wiley Periodicals, Inc.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Wu GJ,Chen JT,Tsai HC,Chen TL,Liu SH,Chen RMdoi
10.1002/jcb.25847subject
Has Abstractpub_date
2017-09-01 00:00:00pages
2635-2644issue
9eissn
0730-2312issn
1097-4644journal_volume
118pub_type
杂志文章abstract：:Extracellular matrix metalloproteinases (MMPs) are activated in dilated cardiomyopathic (DCM) hearts [Tyagi et al. (1996): Mol Cell Biochem 155:13-21]. To examine whether the MMP activation is occurring at the gene expression level, we performed differential display mRNA analysis on tissue from six dilated cardiomyopa...
journal_title：Journal of cellular biochemistry
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更新日期：1996-11-01 00:00:00
abstract：:Cancer prevalence is critically increasing worldwide; accordingly, improved prediction and therapeutic tools are necessary. Interleukin (IL)-21 is a potent antitumor cytokine, and the relationship between its gene variations and cancer risk is well established. Nevertheless, so far no study has investigated its role i...
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更新日期：2015-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/jcb.25735
更新日期：2017-03-01 00:00:00
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journal_title：Journal of cellular biochemistry
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更新日期：2019-06-01 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4644(1998)72:30/31+<238::AID-JC
更新日期：1998-01-01 00:00:00
abstract：:The effects of serum on inositol 1,4,5-trisphosphate (IP3) formation and Ca2+ mobilization in the human submandibular cell line A253 were studied. Exposure of A253 cells to fetal bovine serum (FBS) elicited a 3.3-fold increase in IP3 formation and a concentration-dependent transient increase in cytosolic free Ca2+ con...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1999-06-15 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/(sici)1097-4644(19991201)75:3<369::aid-jcb
更新日期：1999-12-01 00:00:00
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journal_title：Journal of cellular biochemistry
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doi：10.1002/jcb.25919
更新日期：2017-09-01 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.10171
更新日期：2002-01-01 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.10168
更新日期：2002-01-01 00:00:00
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更新日期：2003-12-01 00:00:00
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更新日期：2010-04-15 00:00:00
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doi：10.1002/(SICI)1097-4644(199607)62:1%3C113::AID-JCB
更新日期：1996-07-01 00:00:00
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更新日期：2019-03-01 00:00:00
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更新日期：2010-08-01 00:00:00
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doi：10.1002/jcb.25043
更新日期：2015-05-01 00:00:00
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journal_title：Journal of cellular biochemistry
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doi：10.1002/(sici)1097-4644(19980601)69:3<316::aid-jcb
更新日期：1998-06-01 00:00:00
abstract：:Matrix metalloproteinase-1 (MMP-1) is one of only a few enzymes with the ability to degrade the stromal collagens (types I and III) at neutral pH, and high expression of MMP-1 has been associated with aggressive and invasive cancers. We recently reported a single nucleotide insertion/deletion polymorphism (SNP) in the...
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pub_type： 杂志文章
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更新日期：2002-01-01 00:00:00
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更新日期：2018-02-01 00:00:00
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journal_title：Journal of cellular biochemistry
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doi：
更新日期：1997-01-01 00:00:00
abstract：:microRNA (miRNA) is a family of small, non-coding RNA first discovered as an important regulator of development in Caenorhabditis elegans (C. elegans). Numerous miRNAs have been found in C. elegans, and some of them are well conserved in many organisms. Though, the biologic function of miRNAs in C. elegans was largely...
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pub_type： 杂志文章,评审
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更新日期：2013-05-01 00:00:00
