Horizontal gene transfer versus biostimulation: A strategy for bioremediation in Goa.

Abstract:

:Bioaugmentation, Biostimulation and Horizontal gene transfer (HGT) of catabolic genes have been proven for their role in bioremediation of hydrocarbons. It also has been proved that selection of either biostimulation or bioremediation varies for every contaminated site. The reliability of HGT compared to biostimulation and bioremediation was not tested. The present study focuses on reliability of biostimulatiion, bioaugmentation and HGT during biodegradation of Diesel oil and Non aqueous phase liquids (NAPL). Pseudomonas aeruginosa (AEBBITS1) having alkB and NDO genes was used for bioaugmentation and the experiment was conducted using seawater as medium. Based on Gas chromatography results diesel was found to be degraded to 100% in both presence and absence of AEBBITS1. Denturing gradient gel electrophoresis result showed same pattern in presence and absence of AEBBITS1 indicating no HGT. NAPL degradation was found to be more by Biostimulated Bioaugmentation compared to biostimulation and bioaugmentation alone. This proves that biostimulated bioaugmentation is better strategy for oil contamination (tarabll) in Velsao beach, Goa.

journal_name

Mar Pollut Bull

authors

Pasumarthi R,Mutnuri S

doi

10.1016/j.marpolbul.2016.09.044

subject

Has Abstract

pub_date

2016-12-15 00:00:00

pages

271-276

issue

1-2

eissn

0025-326X

issn

1879-3363

pii

S0025-326X(16)30781-0

journal_volume

113

pub_type

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