Biogas properties and enzymatic analysis during anaerobic fermentation of Phragmites australis straw and cow dung: influence of nickel chloride supplement.

Abstract:

:The importance of nickel (added as NiCl2) on mesophilic anaerobic fermentation of Phragmites australis straw and cow dung was demonstrated by investigating the biogas properties, pH values, organic matter degradation [chemical oxygen demand (COD)] and enzyme activities (cellulase, protease and dehydrogenase) during the fermentation process. The results showed that Ni2+ addition increased the cumulative biogas yields by >18 % by improving the efficiency of first peak stage and bringing forward the second peak stage. The pH values were not significantly influenced by Ni2+ addition (p > 0.05). Biogas yields were associated with variations in COD concentrations rather than momentary concentrations. At the start-up stage of fermentation (4th day), the biogas yields increased gradually together with the increase of dehydrogenase activities at elevated Ni2+ concentrations when cellulase and protease activities were similar in all test groups. It is suggested that Ni2+ addition was mainly dependent on the methanogenic stage. After the start-up stage, the impact of Ni2+ addition on biogas production was mainly dependent on its effect on cellulase activities, rather than protease or dehydrogenase activities.

journal_name

Biodegradation

journal_title

Biodegradation

authors

Tian Y,Zhang H,Chai Y,Wang L,Mi X,Zhang L,Ware MA

doi

10.1007/s10532-016-9774-5

subject

Has Abstract

pub_date

2017-02-01 00:00:00

pages

15-25

issue

1

eissn

0923-9820

issn

1572-9729

pii

10.1007/s10532-016-9774-5

journal_volume

28

pub_type

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