Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis.


:With the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-cyB) of B. bovis, and the levels of the anti-B. bovis antibodies, in blood samples collected from 51 Angus cattle on two different occasions. Samples with the anticoagulant EDTA were used for DNA extraction and without anticoagulant for separation of the blood serum. The quantification of the NC mt-cyB of B. bovis was carried out by the quantitative PCR technique (qPCR), while the anti-B. bovis IgG antibody titers (S/P) were quantified by the ELISA method. The NC and S/P data were log10-transformed to improve the approximation to the normal distribution and were analyzed using mixed models. The correlations between NC mt-cyB and S/P were estimated, as well as the repeatability values for each trait. The results obtained showed the high sensitivity of the techniques, with 100% of the animals being positive for B. bovis, detected by both the serological and molecular tests. The correlations estimated between NC and S/P were low, 0.10 and 0.12, in the first and second collection, respectively. The repeatability estimated for NC was 0.06, whereas for the S/P it was 0.42. The low correlations between S/P and NC in the two collections demonstrated that the variation in the NC value is independent of the level of antibodies. This results indicated that animals with a higher levels of antibodies against B. bovis in the first collection continued to have a higher levels in the second one. However, the very low values for the repeatability value of NC, and for the correlations between S/P and NC, demonstrates that neither NC or S/P could be used to discriminate animals for resistance/susceptibility to B. bovis.


Ticks Tick Borne Dis


Giglioti R,Oliveira HN,Ibelli AMG,Bilhassi TB,Néo TA,Santana CH,Rabelo MD,Machado RZ,de Souza Chagas AC,de Sena Oliveira MC




Has Abstract


2017-03-01 00:00:00














  • Parasitism of mustelids by ixodid ticks (Acari: Ixodidae), Maine and New Hampshire, U.S.A.

    abstract::Ticks collected from mustelids from four counties in Maine and one in New Hampshire were identified after harvest. Of the 18 fishers Martes pennanti Erxleben, two mink Neovison vison Schreber, and one long-tailed weasel Mustela frenata Lichtenstein, 589 ticks were collected and identified. They were identified as, in ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Lubelczyk C,Lacombe EH,Elias SP,Beati L,Rand PW,Smith RP Jr

    更新日期:2014-06-01 00:00:00

  • Multilocus sequence typing using mitochondrial genes (mtMLST) reveals geographic population structure of Ixodes ricinus ticks.

    abstract::The hard tick Ixodes ricinus is the principal vector of Lyme borreliosis (LB) group spirochaetes in Europe, but it also transmits a large number of other microbial pathogens that are of importance to animal and human health. Here, we characterise geographically distinct populations of this important ectoparasite based...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Dinnis RE,Seelig F,Bormane A,Donaghy M,Vollmer SA,Feil EJ,Kurtenbach K,Margos G

    更新日期:2014-03-01 00:00:00

  • Detection of a Borrelia species in questing Gulf Coast ticks, Amblyomma maculatum.

    abstract::Borrelia spp. are agents of Lyme disease and relapsing fever, diseases which use Ixodes hard ticks and Ornithodoros soft ticks, respectively, as primary vectors. Some relapsing fever spirochetes, such as B. miyamotoi, are also found in hard ticks. To date, no Borrelia sp. is known to use the hard tick, Amblyomma macul...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Lee JK,Smith WC,McIntosh C,Ferrari FG,Moore-Henderson B,Varela-Stokes A

    更新日期:2014-06-01 00:00:00

  • Tick-borne diseases in the Union of the Comoros are a hindrance to livestock development: Circulation and associated risk factors.

    abstract::Tick-borne diseases (TBD) occur in many temperate countries and are economically important in most tropical and subtropical areas, affecting dairy and beef cattle, as well as small ruminants. Four major tick-borne diseases have been detected in eastern and southern Africa: East Coast fever (ECF) caused by Theileria pa...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Boucher F,Moutroifi Y,Peba B,Ali M,Moindjie Y,Ruget AS,Abdouroihamane S,Madi Kassim A,Soulé M,Charafouddine O,Cêtre-Sossah C,Cardinale E

    更新日期:2020-01-01 00:00:00

  • Coxiella symbiont in the tick Ornithodoros rostratus (Acari: Argasidae).

    abstract::In the present study, the presence of tick-associated bacteria and protozoa in Ornithodoros rostratus ticks (adults, nymphs, and eggs) from the Pantanal region of Brazil were determined by molecular detection. In these ticks, DNA from protozoa in the genera Babesia and Hepatozoon, and bacteria from the genera Ricketts...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Almeida AP,Marcili A,Leite RC,Nieri-Bastos FA,Domingues LN,Martins JR,Labruna MB

    更新日期:2012-09-01 00:00:00

  • Rickettsial infections in ticks from wild birds in Paraguay.

    abstract::Ticks were collected from wild birds at 3 locations in Paraguay during the South American winter in August and September 2012. In total, 480 birds belonging to 106 species were examined. Overall, 31 (6.5%) birds representing 21 species were found parasitized by ticks which were identified as Amblyomma calcaratum Neuma...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Ogrzewalska M,Literak I,Martins TF,Labruna MB

    更新日期:2014-03-01 00:00:00

  • Molecular detection of Anaplasma marginale infection in carrier cattle.

    abstract::Anaplasma marginale infections are responsible for widespread morbidity and mortality particularly in crossbred and exotic breeds of cattle in the tropics and subtropics. In the present study, a semi-nested PCR assay was utilized for the detection of A. marginale infection in carrier cattle from different areas of Pun...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Singh H,Jyoti,Haque M,Singh NK,Rath SS

    更新日期:2012-02-01 00:00:00

  • Tick-borne pathogens in the European polecat, Mustela putorius and in attached Ixodes hexagonus ticks from Germany.

    abstract::The European polecat, Mustela putorius, occurs almost throughout Europe. However, there is a lack of data on the ectoparasite fauna and the potential role in the circulation of tick-borne pathogens (TBP) of this mustelid species. The aim of this study was to investigate whether M. putorius contributes to the maintenan...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Król N,Obiegala A,Kretschmar FM,Hamel D,Pfeffer M

    更新日期:2019-04-01 00:00:00

  • A soft tick Ornithodoros moubata salivary protein OmCI is a potent inhibitor to prevent avian complement activation.

    abstract::Complement is a key first line innate host defense system in the blood of vertebrates. Upon activation, this powerful defense mechanism can elicit inflammatory responses, lyse non-self-cells, or mark them for opsonophagocytic removal. Blood-feeding arthropods thus require the ability to block host complement activatio...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Frye AM,Hart TM,Tufts DM,Ram S,Diuk-Wasser MA,Kraiczy P,Blom AM,Lin YP

    更新日期:2020-03-01 00:00:00

  • Pathogenic potential of a Costa Rican strain of 'Candidatus Rickettsia amblyommii' in guinea pigs (Cavia porcellus) and protective immunity against Rickettsia rickettsii.

    abstract::'Candidatus Rickettsia amblyommii' is a spotted fever group rickettsia that is not considered pathogenic, although there is serologic evidence of possible infection in animals and humans. The aim of this study was to evaluate the pathogenic potential of a Costa Rican strain of 'Candidatus R. amblyommii' in guinea pigs...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Rivas JJ,Moreira-Soto A,Alvarado G,Taylor L,Calderón-Arguedas O,Hun L,Corrales-Aguilar E,Morales JA,Troyo A

    更新日期:2015-09-01 00:00:00

  • Evidence for an effect of landscape connectivity on Borrelia burgdorferi sensu stricto dispersion in a zone of range expansion.

    abstract::In North America, different strains of the Lyme disease-causing bacterium Borrelia burgdorferi sensu stricto cluster into phylogenetic groups that are associated with different levels of pathogenicity and, for some, specific rodent reservoir hosts. Here we explore whether landscape connectivity, by impacting host disp...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Mechai S,Margos G,Feil EJ,Lindsay LR,Michel P,Kotchi SO,Ogden NH

    更新日期:2018-09-01 00:00:00

  • Thymol action on cells and tissues of the synganglia and salivary glands of Rhipicephalus sanguineus sensu lato females (Acari: Ixodidae).

    abstract::Thymol is a monoterpene present in plants of the families Lamiaceae, Verbenaceae and Apiaceae. Despite its proven acaricidal activity, little is known about the mechanism of action of thymol in ticks. Thus, the aim of this study was to perform a morpho-histochemical analysis of the synganglion and salivary glands of p...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Matos RS,Daemon E,de Oliveira Monteiro CM,Sampieri BR,Marchesini PBC,Delmonte C,Camargo-Mathias MI

    更新日期:2019-02-01 00:00:00

  • Infection with Borrelia chilensis in Ixodes stilesi ticks collected from Pudu puda deer.

    abstract::Lyme borreliosis is a vector-borne zoonosis caused by Borrelia burgdorferi sensu lato species complex spirochetes, which are maintained in transmission cycles among vertebrates and Ixodes ticks. Recently, a new genospecies within this complex, Borrelia chilensis, was described in Ixodes stilesi collected from the envi...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Verdugo C,Jiménez O,Hernández C,Álvarez P,Espinoza A,González-Acuña D

    更新日期:2017-08-01 00:00:00

  • Molecular evidence for the inhibition of cytochrome p450s and cholinesterases in ticks by the repellent DEET.

    abstract::For more than 50 years DEET (N,N-Diethyl-m-toluamide) has been considered the gold standard of repellents. It is applied to the skin or clothing to deter mosquitoes and other blood-sucking invertebrate pests from approaching and/or settling, and ultimately it provides temporary protection from bites. Despite rampant g...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Koloski CW,LeMoine CMR,Klonowski AR,Smith CM,Cassone BJ

    更新日期:2019-04-01 00:00:00

  • Molecular and functional characterization of Bm05br antigen from Rhipicephalus microplus.

    abstract::Rhipicephalus microplus is a cattle-specific tick, causing considerable losses in the livestock industry. The identification of molecules responsible for modulation of host defenses during different parasite stages can help in the development of alternative methods, such as vaccination, to control tick infestations. H...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Alzugaray MF,Parizi LF,Seixas A,Benavides U,da Silva Vaz I Jr

    更新日期:2017-02-01 00:00:00

  • Knowledge and prevention of tick-borne diseases vary across an urban-to-rural human land-use gradient.

    abstract::We sought to determine the behavioral risk of exposure to tick-borne diseases across a human land-use gradient in a region endemic for diseases transmitted by the lone star tick. We measured the knowledge, attitudes, and preventive behaviors of visitors to 14 suburban, exurban, and rural recreational parks. A structur...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Bayles BR,Evans G,Allan BF

    更新日期:2013-06-01 00:00:00

  • Brazilian spotted fever: real-time PCR for diagnosis of fatal cases.

    abstract::Suspicion of Brazilian spotted fever (BSF) should occur in endemic regions upon surveillance of the acute febrile icteric hemorrhagic syndrome (AFIHS). However, limitations associated with currently available laboratory tests pose a challenge to early diagnosis, especially in fatal cases. Two real-time PCR (qPCR) prot...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: dos Santos FC,do Nascimento EM,Katz G,Angerami RN,Colombo S,de Souza ER,Labruna MB,da Silva MV

    更新日期:2012-12-01 00:00:00

  • Preventing tick-bites among children in Indiana, USA: An analysis of factors associated with parental protective behaviors.

    abstract::Despite evidence to the effect that there is low parental adoption of tick-bite personal protective behavior (PPB) for their children - a population at high risk for tick exposure, very limited information is available on factors associated with parental adoption of PPB. The objective of this study was to identify the...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Omodior O,Anderson KR,Clark W,Eze P,Donohoe H

    更新日期:2021-03-01 00:00:00

  • Molecular detection of the human pathogen Rickettsia parkeri strain Atlantic rainforest in Amblyomma ovale ticks in Argentina.

    abstract::Human rickettsioses caused by Rickettsia parkeri strain Atlantic rainforest in Brazil motivated the analysis of Amblyomma ovale ticks in Misiones province, a similar ecological region in northeastern Argentina. During 2010-2017, 393 A. ovale ticks were collected from domestic and wild animals and from vegetation, and ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Lamattina D,Tarragona EL,Nava S

    更新日期:2018-07-01 00:00:00

  • Non-surgical management of spontaneous splenic rupture due to Babesia microti infection.

    abstract::Babesiosis is an emerging tick-borne infection. We describe 2 hospitalized patients from the United States with spontaneous splenic rupture attributable to Babesia microti infection. The first patient was a 36-year-old previously healthy man with a low-grade splenic rupture not requiring surgical intervention. The sec...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: El Khoury MY,Gandhi R,Dandache P,Lombardo G,Wormser GP

    更新日期:2011-12-01 00:00:00

  • Investigating the first outbreak of oriental theileriosis in cattle in South Australia using multiplexed tandem PCR (MT-PCR).

    abstract::This study investigated the first outbreak of oriental theileriosis in a herd of beef cattle in South Australia using a newly established multiplexed tandem PCR (MT-PCR) to identify, differentiate and quantitate the four genotypes (buffeli, chitose, ikeda and type 5) of Theileria orientalis recognised to occur in Aust...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Gebrekidan H,Gasser RB,Perera PK,McGrath S,McGrath S,Stevenson MA,Jabbar A

    更新日期:2015-07-01 00:00:00

  • Genetic variability of Anaplasmataceae circulating in small mammals and ticks in an Ixodes persulcatus/Ixodes trianguliceps sympatric area in Russian Siberia.

    abstract::A total of 705 rodents from Myodes, Microtus, and Apodemus genera, 396 adult questing Ixodes persulcatus, and 115 Ixodes larvae and nymphs taken from rodents (and then molted under laboratory conditions to nymphs and adults) were collected in 2013-2018 in Omsk province, Russian Siberia, and examined for the presence o...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Rar V,Yakimenko V,Tikunov A,Makenov M,Epikhina T,Tancev A,Tikunova N

    更新日期:2020-09-01 00:00:00

  • Identical 18S rRNA haplotypes of Hepatozoon canis in dogs and foxes in Brandenburg, Germany.

    abstract::Hepatozoon canis is a blood parasite of the suborder Adeleorina infecting wild and domestic canids. Transmission occurs by oral uptake of Rhipicephalus sanguineus sensu lato vector ticks infected with H. canis, but vertical transmission is also assumed to be possible. In German foxes, a high prevalence of H. canis has...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Helm CS,Samson-Himmelstjerna GV,Liesner JM,Kohn B,Müller E,Schaper R,Pachnicke S,Schulze C,Krücken J

    更新日期:2020-11-01 00:00:00

  • First molecular evidence of Coxiella burnetii infecting ticks in Cuba.

    abstract::Coxiella burnetii is the causative agent of Q fever. In order to explore the occurrence of C. burnetii in ticks, samples were collected from horses, dogs and humans living in a Cuban occidental community. The species most commonly recovered were Amblyomma mixtum (67%), Rhipicephalus sanguineus s.l. (27%) and Dermacent...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Noda AA,Rodríguez I,Miranda J,Contreras V,Mattar S

    更新日期:2016-02-01 00:00:00

  • Molecular detection and characterization of Hepatozoon spp. in dogs from the central part of Turkey.

    abstract::Canine hepatozoonosis is a tick-borne protozoal disease caused by Hepatozoon spp. Two species of Hepatozoon are currently known to infect dogs as Hepatozoon canis and H. americanum. Although H. canis generally causes a chronic infection with relatively mild clinical alterations compared to H. americanum, infection by ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Aydin MF,Sevinc F,Sevinc M

    更新日期:2015-04-01 00:00:00

  • A golden jackal (Canis aureus) from Austria bearing Hepatozoon canis--import due to immigration into a non-endemic area?

    abstract::The protozoan Hepatozoon canis, which is transmitted via ingestion of infected ticks by canine hosts, is not endemic to mid-latitude regions in Europe. Its distribution is supposed to be linked to the occurrence of its primary tick vector Rhipicephalus sanguineus. A young male golden jackal (Canis aureus) found as roa...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Duscher GG,Kübber-Heiss A,Richter B,Suchentrunk F

    更新日期:2013-02-01 00:00:00

  • Molecular detection and phylogeny of Anaplasma spp. in cattle reveals the presence of novel strains closely related to A. phagocytophilum in Turkey.

    abstract::Anaplasma species are obligate intracellular rickettsial pathogens that affect the health of humans and animals. In this study, we investigated the presence and frequency of Anaplasma species by 16S rRNA PCR-RLB, RFLP, and DNA sequencing in 200 apparently healthy cattle. Anaplasma spp. overall infection rate was 38.5 ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Aktas M,Çolak S

    更新日期:2021-01-01 00:00:00

  • Lyme borreliae prevalence and genospecies distribution in ticks removed from humans.

    abstract::Lyme borreliosis (LB) is the most important human tick-borne disease, but Borrelia genospecies cause different clinical manifestations. Ticks of the genus Ixodes removed from humans between 2006 and 2012 were analysed for Borrelia burgdorferi sensu lato (sl) infections. The majority of ticks originated from the Greate...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Waindok P,Schicht S,Fingerle V,Strube C

    更新日期:2017-08-01 00:00:00

  • First molecular detection of Rickettsia parkeri in Amblyomma tigrinum and Amblyomma dubitatum ticks from Uruguay.

    abstract::Rickettsia parkei is the etiological agent of spotted fever in Uruguay, where is transmitted to humans by the tick Amblyomma triste. In the present study, ticks were collected from capybaras (Hydrochoerus hydrochaeris) and domestic dogs during 2011-2012 in different parts of Uruguay. Three out of 11 (27.3%) Amblyomma ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Lado P,Castro O,Labruna MB,Venzal JM

    更新日期:2014-10-01 00:00:00

  • Note on Ehrlichia chaffeensis, Ehrlichia ewingii, and "Borrelia lonestari" infection in lone star ticks (Acari: Ixodidae), Nebraska, USA.

    abstract::The lone star tick, Amblyomma americanum (L.) (Acari: Ixodidae), is established in southeastern Nebraska yet the prevalence of tick-associated microorganisms is not known. An initial PCR-based analysis for Ehrlichia chaffeensis, Ehrlichia ewingii, and Borrelia infection in host-seeking adult ticks collected in southea...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章


    authors: Maegli A,Loy JD,Cortinas R

    更新日期:2016-02-01 00:00:00