Investigating intracranial tumour growth patterns with multiparametric MRI incorporating Gd-DTPA and USPIO-enhanced imaging.


:High grade and metastatic brain tumours exhibit considerable spatial variations in proliferation, angiogenesis, invasion, necrosis and oedema. Vascular heterogeneity arising from vascular co-option in regions of invasive growth (in which the blood-brain barrier remains intact) and neoangiogenesis is a major challenge faced in the assessment of brain tumours by conventional MRI. A multiparametric MRI approach, incorporating native measurements and both Gd-DTPA (Magnevist) and ultrasmall superparamagnetic iron oxide (P904)-enhanced imaging, was used in combination with histogram and unsupervised cluster analysis using a k-means algorithm to examine the spatial distribution of vascular parameters, water diffusion characteristics and invasion in intracranially propagated rat RG2 gliomas and human MDA-MB-231 LM2-4 breast adenocarcinomas in mice. Both tumour models presented with higher ΔR1 (the change in transverse relaxation rate R1 induced by Gd-DTPA), fractional blood volume (fBV) and apparent diffusion coefficient than uninvolved regions of the brain. MDA-MB-231 LM2-4 tumours were less densely cellular than RG2 tumours and exhibited substantial local invasion, associated with oedema, whereas invasion in RG2 tumours was minimal. These additional features were reflected in the more heterogeneous appearance of MDA-MB-231 LM2-4 tumours on T2 -weighted images and maps of functional MRI parameters. Unsupervised cluster analysis separated subregions with distinct functional properties; areas with a low fBV and relatively impermeable blood vessels (low ΔR1 ) were predominantly located at the tumour margins, regions of MDA-MB-231 LM2-4 tumours with relatively high levels of water diffusion and low vascular permeability and/or fBV corresponded to histologically identified regions of invasion and oedema, and areas of mismatch between vascular permeability and blood volume were identified. We demonstrate that dual contrast MRI and evaluation of tissue diffusion properties, coupled with cluster analysis, allows for the assessment of heterogeneity within invasive brain tumours and the designation of functionally diverse subregions that may provide more informative predictive biomarkers.


NMR Biomed


NMR in biomedicine


Boult JK,Borri M,Jury A,Popov S,Box G,Perryman L,Eccles SA,Jones C,Robinson SP




Has Abstract


2016-11-01 00:00:00












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    journal_title:NMR in biomedicine

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    authors: Sack M,Wetterling F,Sartorius A,Ende G,Weber-Fahr W

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  • DTI of human skeletal muscle: the effects of diffusion encoding parameters, signal-to-noise ratio and T2 on tensor indices and fiber tracts.

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    authors: Froeling M,Nederveen AJ,Nicolay K,Strijkers GJ

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    journal_title:NMR in biomedicine

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    authors: Wray S,Wilkie DR

    更新日期:1992-05-01 00:00:00

  • In vivo and in vitro bi-exponential diffusion of N-acetyl aspartate (NAA) in rat brain: a potential structural probe?

    abstract::Diffusion measurements were performed on the N-acetyl aspartate (NAA) signal in in situ brains (in vivo and post-mortem) and on in vitro brain tissue at 37 degrees C using wide ranges of b-values (from 0 up to 4.5 x 10(6) s/cm2 and 35.8 x 10(6) s/cm2 for the in vivo and the in vitro cases, respectively). In vivo and i...

    journal_title:NMR in biomedicine

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    authors: Assaf Y,Cohen Y

    更新日期:1998-04-01 00:00:00

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    abstract::Quantitative analysis of extracts from two Ehrlich ascites tumour cell lines (Lettre cells) by 19F NMR in vitro demonstrated that one Lettre cell line (designated LLM) metabolized 30-50% less 5-fluorouracil to 5-fluoronucleotides when compared to the other cell line (designated LHM). HPLC analysis of these cellular ex...

    journal_title:NMR in biomedicine

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    authors: McSheehy PM,Maxwell RJ,Griffiths JR

    更新日期:1991-12-01 00:00:00

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    abstract::Spatiotemporally structured noise, such as physiological noise, is a potential source of artifacts in functional magnetic resonance imaging (fMRI) and is the main limiting factor for the detection of small blood oxygen level-dependent (BOLD) signal variations. fMRI was employed to detect low-frequency BOLD signal fluc...

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    authors: Goerke U,Möller HE,Norris DG,Schwarzbauer C

    更新日期:2005-12-01 00:00:00

  • Dynamic multivoxel-localized 31 P MRS during plantar flexion exercise with variable knee angle.

    abstract::Exercise studies investigating the metabolic response of calf muscles using 31 P MRS are usually performed with a single knee angle. However, during natural movement, the distribution of workload between the main contributors to force, gastrocnemius and soleus is influenced by the knee angle. Hence, it is of interest ...

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    authors: Niess F,Fiedler GB,Schmid AI,Laistler E,Frass-Kriegl R,Wolzt M,Moser E,Meyerspeer M

    更新日期:2018-06-01 00:00:00

  • Continuous-wave saturation considerations for efficient xenon depolarization.

    abstract::The combination of hyperpolarized Xe with chemical exchange saturation transfer (Hyper-CEST) is a powerful NMR technique to detect highly dilute concentrations of Xe binding sites using RF saturation pulses. Crucially, that combination of saturation pulse strength and duration that generates the maximal Hyper-CEST eff...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章


    authors: Kunth M,Witte C,Schröder L

    更新日期:2015-06-01 00:00:00

  • Integrated Laplacian-based phase unwrapping and background phase removal for quantitative susceptibility mapping.

    abstract::Quantitative susceptibility mapping (QSM) is a recently developed MRI technique that provides a quantitative measure of tissue magnetic susceptibility. To compute tissue magnetic susceptibilities based on gradient echoes, QSM requires reliable unwrapping of the measured phase images and removal of contributions caused...

    journal_title:NMR in biomedicine

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    authors: Li W,Avram AV,Wu B,Xiao X,Liu C

    更新日期:2014-02-01 00:00:00

  • 13C NMR kinetic studies of the rapid stimulation of glucose metabolism by estrogen in immature rat uterus.

    abstract::Glucose metabolism in control and estrogen stimulated rat uteri was investigated using 13C NMR spectroscopy. By employing an NMR adapted perifusion system, and developing protocols and methods based on the application of [1-13C]glucose labeling, it was possible to measure, with a temporal resolution of 10 min, the kin...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章


    authors: Shinkarenko L,Kaye AM,Degani H

    更新日期:1994-08-01 00:00:00

  • Effect of phosphate electrolyte buffer on the dynamics of water in tendon and cartilage.

    abstract::A number of NMR spectroscopic and microscopic MRI (microMRI) techniques were used to study proton dynamics in canine tendon and articular cartilage immersed in normal saline solution (NaCl solution) and high-concentration phosphate-buffered saline (PBS) solution. In a proton CPMG experiment on tendons, the T(2) relaxa...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章


    authors: Zheng S,Xia Y

    更新日期:2009-02-01 00:00:00

  • Metabolite-specific NMR spectroscopy in vivo.

    abstract::An outline is presented of metabolite-specific in vivo NMR spectroscopy (particularly in brain). It reviews from a physical spectroscopist's perspective, the need for and the methods of observation of, individual metabolite resonances. ...

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    authors: Allen PS,Thompson RB,Wilman AH

    更新日期:1997-12-01 00:00:00

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    abstract::Evaluation of high intensity focused ultrasound (HIFU) treatment with MRI is generally based on assessment of the non-perfused volume from contrast-enhanced T1-weighted images. However, the vascular status of tissue surrounding the non-perfused volume has not been extensively investigated with MRI. In this study, clus...

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    authors: Jacobs I,Hectors SJ,Schabel MC,Grüll H,Strijkers GJ,Nicolay K

    更新日期:2015-11-01 00:00:00

  • Longitudinal assessment of tissue properties and cardiac diffusion metrics of the ex vivo porcine heart at 7 T: Impact of continuous tissue fixation using formalin.

    abstract::In this study we aimed to assess the effects of continuous formalin fixation on diffusion and relaxation metrics of the ex vivo porcine heart at 7 T. Magnetic resonance imaging was performed on eight piglet hearts using a 7 T whole body system. Hearts were measured fresh within 3 hours of cardiac arrest followed by im...

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    authors: Lohr D,Terekhov M,Veit F,Schreiber LM

    更新日期:2020-07-01 00:00:00

  • Regional neurochemical profiles in the human brain measured by ¹H MRS at 7 T using local B₁ shimming.

    abstract::Increased sensitivity and chemical shift dispersion at ultra-high magnetic fields enable the precise quantification of an extended range of brain metabolites from (1)H MRS. However, all previous neurochemical profiling studies using single-voxel MRS at 7 T have been limited to data acquired from the occipital lobe wit...

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    pub_type: 杂志文章


    authors: Emir UE,Auerbach EJ,Van De Moortele PF,Marjańska M,Uğurbil K,Terpstra M,Tkáč I,Oz G

    更新日期:2012-01-01 00:00:00

  • Improvement of resolution for brain coupled metabolites by optimized (1)H MRS at 7T.

    abstract::Resolution enhancement for glutamate (Glu), glutamine (Gln) and glutathione (GSH) in the human brain by TE-optimized point-resolved spectroscopy (PRESS) at 7 T is reported. Sub-TE dependences of the multiplets of Glu, Gln, GSH, γ-aminobutyric acid (GABA) and N-acetylaspartate (NAA) at 2.2-2.6 ppm were investigated wit...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章


    authors: Choi C,Dimitrov IE,Douglas D,Patel A,Kaiser LG,Amezcua CA,Maher EA

    更新日期:2010-11-01 00:00:00

  • Improvements in high-field localized MRS of the medial temporal lobe in humans using new deformable high-dielectric materials.

    abstract::The intrinsic nonuniformities in the transmit radiofrequency field from standard quadrature volume resonators at high field are particularly problematic for localized MRS in areas such as the temporal lobe, where a low signal-to-noise ratio and poor metabolite quantification result from destructive B₁⁺ field interfere...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章


    authors: Snaar JE,Teeuwisse WM,Versluis MJ,van Buchem MA,Kan HE,Smith NB,Webb AG

    更新日期:2011-08-01 00:00:00

  • A 31P study of fatigue and metabolism in human skeletal muscle with voluntary, intermittent contractions at different forces.

    abstract::Normal subjects performed voluntary, isometric exercise 1 s contraction, 1 s rest for 10 min) of the first dorsal interosseous (FDI) muscle with a target force of 25, 50 and 100% of the maximal voluntary contraction (MVC) force. 31P NMR spectra were collected continuously before, during and after exercise. Data were a...

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    authors: Newham DJ,Cady EB

    更新日期:1990-10-01 00:00:00

  • Fast multicomponent 3D-T1ρ relaxometry.

    abstract::NMR relaxometry can provide information about the relaxation of the magnetization in different tissues, increasing our understanding of molecular dynamics and biochemical composition in biological systems. In general, tissues have complex and heterogeneous structures composed of multiple pools. As a result, bulk magne...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章


    authors: Zibetti MVW,Helou ES,Sharafi A,Regatte RR

    更新日期:2020-12-01 00:00:00

  • Assessment of cholesteryl ester transfer protein function in lipoprotein mixtures by 1H NMR spectroscopy.

    abstract::Studies of cholesteryl ester transfer protein (CETP) function in lipoprotein mixtures pose many difficulties by conventional biochemical methods. For instance, studies on the effects of CETP on the composition of apolipoprotein B containing lipoproteins (very low and low density lipoproteins) in lipoprotein mixtures a...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章


    authors: Korhonen A,Ala-Korpela M,Liinamaa MJ,Jokisaari J,Kesäniemi YA,Savolainen MJ

    更新日期:1997-10-01 00:00:00

  • Interventional MRI-guided local delivery of agents into swine bile duct walls using MR-compatible needle-integrated balloon catheter system.

    abstract::The purpose of this study was to investigate the feasibility of interventional MRI-guided local agent delivery into pig common bile duct (CBD) walls using a newly designed MR-compatible, needle-integrated balloon catheter system. We first designed a needle-integrated balloon catheter system that comprised of a 22 G MR...

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    pub_type: 杂志文章


    authors: Zhang F,Bai Z,Shi Y,Wang J,Li Y,Yang X

    更新日期:2015-06-01 00:00:00

  • Multilabeled 13C substrates as probes in in vivo 13C and 1H NMR spectroscopy.

    abstract::The potential use of 13C multilabeled substrates has been studied in biological applications using in vivo and in vitro proton and 13C NMR spectroscopy. In 13C NMR spectroscopy, multilabeled compounds allow the simultaneous observation of several nuclei or increase distinctly the signal to noise ratio due to a higher ...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章


    authors: Künnecke B,Cerdan S

    更新日期:1989-12-01 00:00:00

  • In vivo 31P MRS of experimental tumours.

    abstract::More than 50% of cancers fail to respond to any individual treatment and tumour follow-up after treatment plays a major role in routine therapy planning and pharmacological research. Today, MRS is the only technological approach providing non-invasive access to tumour biochemistry. Ten years ago, expectations were rai...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章,评审


    authors: de Certaines JD,Larsen VA,Podo F,Carpinelli G,Briot O,Henriksen O

    更新日期:1993-11-01 00:00:00

  • Studies of metabolic compartmentation and glucose transport using in vivo MRS.

    abstract::Organs consist of several types of cells with specialized functions. This cellular localization of function is often referred to as compartmentation. Due to the intrinsic low sensitivity of MR methods it is generally not possible in vivo to obtain images or spectra of single cells. Instead the MRS signal is the sum of...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章,评审


    authors: Rothman DL

    更新日期:2001-04-01 00:00:00

  • Amplification or obfuscation: is localization improving our clinical understanding of phosphorus metabolism?

    abstract::Localization techniques are considered an indispensable part of the clinical spectroscopy examination. The results are often a marked improvement in diagnostic information (amplification), but because of the introduction of new artifacts and the more prolonged examination time, some of the undoubted advantage is lost ...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章,评审


    authors: Ross B,Narasimhan PT,Tropp J,Derby KS

    更新日期:1989-12-01 00:00:00

  • Health and infarcted brain tissues studied at short diffusion times: the origins of apparent restriction and the reduction in apparent diffusion coefficient.

    abstract::The significance of NMR water diffusion measurements performed at short diffusion times (< 10 ms) for brain tissue is examined. An apparent restriction to diffusion for both healthy and cytotoxically edematous tissue is shown: cytotoxic edema lengthens the diffusion time at which this phenomenon is visible. The dramat...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章


    authors: Norris DG,Niendorf T,Leibfritz D

    更新日期:1994-11-01 00:00:00

  • The interaction between apparent diffusion coefficients and transverse relaxation rates of human brain metabolites and water studied by diffusion-weighted spectroscopy at 7 T.

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    journal_title:NMR in biomedicine

    pub_type: 杂志文章


    authors: Branzoli F,Ercan E,Webb A,Ronen I

    更新日期:2014-05-01 00:00:00

  • Lithium ion as a probe of Na+ channel activity in isolated rat hearts: a multinuclear NMR study.

    abstract::The aim of this study was to analyze Na+ fluxes in whole perfused hearts using Li+ as a Na+ congener and 7Li-nuclear magnetic resonance as a detection method. Hearts were equilibrated for 32 min with 15 mM LiCl added to P1-free Krebs-Henseleit buffer (intracellular space (ICS) [Li+] = 21.5 +/- 3.4 mM). Li+ efflux was ...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章


    authors: Kupriyanov VV,Xiang B,Yang L,Deslauriers R

    更新日期:1997-09-01 00:00:00

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    journal_title:NMR in biomedicine

    pub_type: 杂志文章,评审


    authors: Katscher U,van den Berg CAT

    更新日期:2017-08-01 00:00:00

  • Proton T2 relaxation of cerebral metabolites of normal human brain over large TE range.

    abstract::T2 of NAA, creatine and choline-containing compounds were measured in posterior frontal white matter and occipital grey matter in 10 healthy human volunteers. Decay curves comprised signals from eight TE times ranging from 30 to 800 ms with TR 2000 ms acquired with a PRESS sequence on a 1.5 T clinical scanner. Simulat...

    journal_title:NMR in biomedicine

    pub_type: 临床试验,杂志文章


    authors: Brief EE,Whittall KP,Li DK,MacKay AL

    更新日期:2005-02-01 00:00:00