G-CSF mobilized peripheral blood stem cell collection for allogeneic transplantation in healthy donors: Analysis of factors affecting yield.


:Mobilized PBSC are the main source for allogeneic HSCT. We aimed to evaluate factors that affect CD34+ cell yield including the donor's age, gender, BSA, processed blood volume and the method of G-CSF dose calculation. Data from 170 healthy donors were analyzed. The concentration of CD34+ cells in the peripheral blood (PB) and the processed volume of blood were significantly correlated to CD34+ cells yield (P < .00005 and P < .001, respectively). The G-CSF dose per m2 was significantly correlated to the concentration of CD34+ cells in the PB (P = .0003) and in the product (P = .01). Smaller BSA and less processed volume were found among female donors, who were given lesser G-CSF dose per m2 , and showed lower yield compared to men. However, multivariate analysis of the yield showed that only the concentration of CD34+ cells in the PB and the processed volume remained independent significant.


J Clin Apher


Machaczka M,Hägglund H,Staver E,Joks M,Hassan M,Wahlin BE,Axdorph Nygell U




Has Abstract


2017-12-01 00:00:00












  • Higher efficacy of intermediate dose cytarabine + G-CSF compared to cyclophosphamide + G-CSF in hematopoietic stem cell mobilization in patients with multiple myeloma.

    abstract:BACKGROUND:There are several regimens used in hematopoietic stem cell (HSC) mobilization in multiple myeloma (MM). Cyclophosphamide (Cy) is one of the most commonly used agents, although it does not always result in collecting adequate number of CD34+ cells. Recently, cytarabine (Ara-C) has been proposed as potentially...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Bogucka-Fedorczuk A,Czyz A,Kalicińska E,Sawicki M,Laszkowska-Lewko M,Wicherska-Pawłowska K,Rybka J,Szeremet A,Prajs I,Szymczak D,Wróbel T

    更新日期:2020-08-01 00:00:00

  • Efficacy of plasmapheresis and semi-selective immunoadsorption for removal of anti-HLA antibodies.

    abstract:BACKGROUND:In organ transplantation, apheresis is frequently used for removal of anti-HLA antibodies. However, it is unclear whether plasmapheresis (PP) or semi-selective immunoadsorption (IA) should be employed, and the optimal number of apheresis sessions required to reach post-treatment objectives is also unknown. ...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Jambon F,Merville P,Guidicelli G,Taton B,De Précigout V,Couzi L,Moreau K,Visentin J

    更新日期:2020-11-30 00:00:00

  • Therapeutic plasma exchange for acute inflammatory demyelinating syndromes of the central nervous system.

    abstract::Idiopathic inflammatory demyelinating diseases (IIDDs) of the central nervous system, of which multiple sclerosis is the prototype, represent a family of monophasic, recurrent or progressive diseases with overlapping clinical and pathological manifestations. While most patients recover spontaneously or following a bri...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章,评审


    authors: Weinshenker BG

    更新日期:1999-01-01 00:00:00

  • Peripheral blood stem cell transplantation: impact on procedure load and workload in an apheresis unit.

    abstract::Peripheral blood stem cells (PBSC) reinfusion appears to hasten hematologic reconstitution following myeloablative therapy. While procurement of PBSC adds apheresis procedures, rapid engraftment could decrease the demand for platelet transfusions. To determine the impact of PBSC collection on workload in our apheresis...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Ghalie R,McLeod B,Richman C,Valentino L,Manson S,Netols C,Kaizer H

    更新日期:1992-01-01 00:00:00

  • Successful management of a Jehovah's Witness with thrombotic thrombocytopenic purpura unwilling to be treated with therapeutic plasma exchange.

    abstract::Thrombotic thrombocytopenic purpura (TTP) is a life-threatening disease treated successfully with plasma exchange. Jehovah's Witnesses whose religious beliefs preclude them from accepting plasma exchange may require alternative forms of therapy. We report a case of one such patient who presented with TTP, whom we succ...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Dabak V,Kuriakose P,Raman S

    更新日期:2007-01-01 00:00:00

  • Continuous-flow techniques for platelet concentrate collection: a step toward standardization and yield predictability.

    abstract::This study chronicles leukocyte- and erythrocyte-depleted platelet concentrate collection by a dual stage channel in which three variables: 1) donor peripheral blood platelet concentration, 2) total blood processed, and 3) collection volume were statistically correlated with platelet yield as determined by a multiple ...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Hester JP,Kellogg RM,Mulzet AP,Freireich EJ

    更新日期:1985-01-01 00:00:00

  • Extracorporeal photopheresis reduces the number of mononuclear cells that produce pro-inflammatory cytokines, when tested ex-vivo.

    abstract::Extracorporeal photopheresis (ECP) has been shown to be clinically effective in the treatment of many T cell-mediated conditions. ECP's mechanism of action includes the induction of apoptosis and the release of pro-inflammatory cytokines. Recently, we have observed early lymphoid apoptosis, detectable immediately post...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Bladon J,Taylor P

    更新日期:2002-01-01 00:00:00

  • Research and development in the blood bank: efforts of a hospital donor center to improve efficiency of in-line leukocyte reduction filters for automated plateletpheresis.

    abstract::In-line leukocyte reduction filters (LRF) are available for use with an automated plateletpheresis (PPH) system. Initially, 62% of PPH units produced with such a filter (LRF6, N = 29) had postfiltration (POF) white blood cell (WBC) counts < 5 x 10(6), with a mean POF WBC of 42 x 10(6). In an attempt to decrease POF WB...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Drew MJ,Hodges J

    更新日期:1995-01-01 00:00:00

  • Clinically relevant hypokalaemia, hypocalcaemia, and loss of hemoglobin and platelets during stem cell apheresis.

    abstract::Since the introduction of hematopoietic growth factors, the collection of mobilized stem cells via leukapheresis has widely replaced the harvest of bone marrow in both autologous and allogeneic transplantation settings. We investigated the frequency and the extent of anticoagulant-induced electrolyte changes and the c...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Schlenke P,Frohn C,Steinhardt MM,Kirchner H,Klüter H

    更新日期:2000-01-01 00:00:00

  • Aluminium loading during therapeutic plasma exchange.

    abstract::Graphite furnace atomic absorption spectroscopy was used to demonstrate a fivefold increase in the median plasma aluminium levels of four patients undergoing therapeutic plasmapheresis. This plasma aluminium loading resulted from contamination of albumin incorporated in the replacement fluids and took place during its...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Monteagudo F,Wood L,Jacobs P,Folb P,Cassidy M

    更新日期:1987-01-01 00:00:00

  • Management of systemic unfractionated heparin anticoagulation during therapeutic plasma exchange.

    abstract:BACKGROUND:Therapeutic plasma exchange (TPE) may remove medications from the patient's plasma. Data is limited on the effect of TPE on unfractionated heparin (UFH). STUDY DESIGN AND METHODS:A retrospective review was performed of patients receiving TPE and continuous IV infusion UFH from 1/1/2008 to 6/30/2010. TPE wit...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Kaplan A,Raut P,Totoe G,Morgan S,Zantek ND

    更新日期:2016-12-01 00:00:00

  • Tempo of hematologic recovery correlates with peripheral blood CD34+ cell level in patients undergoing stem cell mobilization.

    abstract::This study was undertaken to evaluate the relationship between the time to recovery of peripheral blood counts and CD34+ cells in the peripheral blood (PB) and apheresis collections of patients undergoing intensive chemotherapy followed by rhG-CSF. Twenty-three patients with a median age of 42 years (range 17-64) with...

    journal_title:Journal of clinical apheresis

    pub_type: 临床试验,杂志文章


    authors: Williams O,Demirer T,Lilleby K,Buckner CD,Bensinger WI

    更新日期:1998-01-01 00:00:00

  • Prediction of immunoglobulin M reduction via therapeutic dose of simple plasma exchange and double filtration plasmapheresis using membrane separation in patients with hyperviscosity syndrome caused by Waldenstrom macroglobulinemia.

    abstract:BACKGROUND:Plasma exchange (PE) and double filtration plasmapheresis (DFPP) are known as effective treatment options for hyperviscosity syndrome (HVS) caused by Waldenstrom macroglobulinemia. Nonetheless, few data are available for the relation between the prescribed dose of apheresis and the reduction rate of target m...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Miyamoto Y,Hamasaki Y,Matsumoto A,Doi K,Noiri E,Nangaku M

    更新日期:2018-10-01 00:00:00

  • Immune globulin intravenous replacement after plasma exchange.

    abstract::The infusion of high-dose (275 mg/kg body weight) immune globulin intravenous (IGIV) after 466 plasma exchanges in 64 patients with autoimmune disease was studied. Side effects occurred during 15% of IGIV infusions. For the most part they were transient and mild, and could be controlled by slowing the infusion rate. T...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Dau PC

    更新日期:1983-01-01 00:00:00

  • The use of fluid boluses to safely perform extracorporeal photopheresis (ECP) in low-weight children: a novel procedure.

    abstract::Apheresis procedures in small children are technically challenging and require special planning with attention to extracorporeal volume. Discontinuous procedures such as extracorporeal photopheresis (ECP) require additional consideration. Alternative methods to perform ECP have been utilized in small children that req...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Schneiderman J,Jacobsohn DA,Collins J,Thormann K,Kletzel M

    更新日期:2010-01-01 00:00:00

  • Prospective change control analysis of transfer of platelet concentrate production from a specialized stem cell transplantation unit to a blood transfusion center.

    abstract:BACKGROUND AND OBJECTIVES:Specialized centers claim a need for blood component production independent from the general blood transfusion services. We performed a prospective change control analysis of the transfer of platelet (PLT) production for hematological patients at the University Hospital Basel from the Departme...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Sigle JP,Medinger M,Stern M,Infanti L,Heim D,Halter J,Gratwohl A,Buser A

    更新日期:2012-01-01 00:00:00

  • Effects of preoperative leukocytapheresis on inflammatory cytokines following surgery for ulcerative colitis: a prospective randomized study.

    abstract::Targeted extracorporeal granulocyte and monocyte apheresis (GMA) has produced clinical efficacy together with down modulation of specific inflammatory cytokines in patients with ulcerative colitis (UC). This study was to investigate if preoperative GMA produces immunological effect on dysregulated immune activity afte...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章,随机对照试验


    authors: Bamba T,Yamamoto T,Umegae S,Matsumoto K

    更新日期:2014-04-01 00:00:00

  • Plasma exchange conditioning for ABO-incompatible renal transplantation.

    abstract::The supply of deceased donor kidneys is inadequate to meet demand. To expand the pool of potential donors, ABO-incompatible transplants from living donors have been performed. We present the Mayo Clinic experience with such transplants. Enrollment was open to patients when the only available potential living kidney do...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Winters JL,Gloor JM,Pineda AA,Stegall MD,Moore SB

    更新日期:2004-01-01 00:00:00

  • Method for the safe and rapid pretransfusion warming of stored blood: an in vitro and in vivo evaluation of a radiofrequency (RF) instrument.

    abstract::Inadvertent hypothermia due to massive infusion of stored blood can be prevented by pretransfusion warming. One approach is the heating of individual packs by means of electromagnetic conduction, which is a method safely used over the last 25 years. The prototype instrument, which has now been re-engineered, can effec...

    journal_title:Journal of clinical apheresis

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Harrison GG,Bird AR,Jacobs P,Coghlan P,Byrne MJ,Ozinsky J,Besseling JL

    更新日期:1992-01-01 00:00:00

  • Computerization of plateletpheresis quality control records with a commercially available spreadsheet program.

    abstract::Many apheresis units lack the resources to acquire customized computer software for record keeping. We have adapted a commercially available "spreadsheet" program (Lotus 1-2-3) to aid in quality control activities for plateletpheresis. Data are entered in a grid pattern wherein each donation occupies one row and succe...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: McLeod BC,Viernes A,Reed S,Sassetti RJ

    更新日期:1990-01-01 00:00:00

  • Effects of LDL-immunoapheresis on plasma concentrations of vitamin E and carotenoids in patients with familial hypercholesterolemia.

    abstract::Recently very potent extracorporeal cholesterol-lowering treatment options have become available for patients with hypercholesterolemia. LDL immunoapheresis treatment selectively removes LDL and lipoprotein(a) from the circulation. Since LDL is the major carrier of lipophilic antioxidants in plasma, the purpose of the...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Cauza E,Jansen M,Resch U,Dunky A,Derfler K,Winklhofer-Roob BM,Kostner K

    更新日期:2004-01-01 00:00:00

  • Hematopoietic progenitor cell count, but not immature platelet fraction value, predicts successful harvest of autologous peripheral blood stem cells.

    abstract::Mobilized stem cells in the peripheral blood (PB) must be efficiently harvested at the appropriate time before autologous PB stem cell (PBSC) transplantation. Enumeration of CD34+ cells in the PB before apheresis predicts the number of PBSCs that can be collected, but the cytometric techniques used are complex and exp...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Mitani N,Yujiri T,Tanaka Y,Tanaka M,Fujii Y,Hinoda Y,Tanizawa Y

    更新日期:2011-01-01 00:00:00

  • Cryopreserved ECP-treated lymphocytes maintain apoptotic response and anti-proliferative effect.

    abstract:BACKGROUND:The ability to cryopreserve a portion of the cells treated during extracorporeal photopheresis (ECP) would improve therapy logistics, particularly for pediatric patients, by allowing multiple therapeutic doses to be collected from a single apheresis session. However, the effect of cryopreservation on ECP-tre...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Radwanski K,Heber C,Min K

    更新日期:2015-06-01 00:00:00

  • Report of the ASFA apheresis registry study on Wilson's disease.

    abstract:PURPOSE:Wilson's disease is a rare autosomal recessive genetic disorder that results in accumulation of copper in the liver, brain, cornea and kidney. Therapeutic plasma exchange (TPE) has been used to remove copper and provide a bridge to liver transplantation. We report here the collective experiences through the ASF...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章,多中心研究


    authors: Pham HP,Schwartz J,Cooling L,Hofmann JC,Kim HC,Morgan S,Pagano MB,Schneiderman J,Winters JL,Yamada C,Wong EC,Wu Y

    更新日期:2016-02-01 00:00:00

  • Reactivity of pretransplant cytotoxic antibodies to a selected HLA panel is not influenced by cyclosporin A, with or without plasma exchange.

    abstract::The influence of 6 weeks of cyclosporin A (CYA), followed by a further 6 weeks of this agent in combination with plasma exchange (PE), was defined on panel reactivity and titre of preformed cytotoxic antibodies in stable patients on haemodialysis awaiting renal transplantation. Nine individuals with antibodies to 30% ...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Swanepoel CR,Cassidy MJ,May M,Oudshoorn M,du Toit E,Wood L,Jacobs P

    更新日期:1991-01-01 00:00:00

  • Indications for and outcomes of therapeutic plasma exchange after cardiac transplantation: A single center retrospective study.

    abstract:INTRODUCTION:Limited data are available describing indications for and outcomes of therapeutic plasma exchange (TPE) in cardiac transplantation. METHODS:In a retrospective study of patients who underwent cardiac transplantation at Duke University Medical Center from 2010 to 2014, we reviewed 3 TPE treatment patterns: ...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Onwuemene OA,Grambow SC,Patel CB,Mentz RJ,Milano CA,Rogers JG,Metjian AD,Arepally GM,Ortel TL

    更新日期:2018-08-01 00:00:00

  • Erythrocytapheresis is a valid and safe therapeutic option in dysmetabolic iron overload syndrome.

    abstract::Dysmetabolic iron overload syndrome is a rare event causing hepatic impairment with serious long-term side effects. Here, we describe a case of metabolic syndrome-related hepatic iron overload that showed a rapid, effective, and safe response to erythrocytapheresis. J. Clin. Apheresis 31:443-447, 2016. © 2015 Wiley Pe...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Vetro C,Rosso R,Palmucci S,Russello M,Colletta G,Romeo MA,Ximenes B,Romano A,Palumbo GA,Di Raimondo F

    更新日期:2016-10-01 00:00:00

  • Debunking superstition: Analyzing the cloud phenomenon in apheresis-Results of a 35-month study.

    abstract::Many practitioners believe in the phenomenon of being labeled either a "black cloud" or "white cloud" while on-call. A "white-cloud" physician is usually defined as one who sees fewer cases while a "black-cloud" is one who often receives more cases. To evaluate these phenomena, a 35-month prospective study was designe...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Staley EM,Raju D,Williams LA 3rd,Kim CH,Jiang N,Pham HP

    更新日期:2018-10-01 00:00:00

  • Improved red cell quality after erythroplasmapheresis with MCS-3P.

    abstract::The cell separator MCS-3P is an apheresis system offering the flexibility to collect standardized red blood cells, plasma, and/or platelets from one donor. Two different programs were used for the red cell apheresis--RBCP (collection of one unit of red cells and two units of plasma) and RBCPS (one unit of red cells an...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Matthes G,Tofote U,Krause KP,Pawlow I,Kucera W,Lerche D

    更新日期:1994-01-01 00:00:00

  • Therapeutic plasma exchange may adjust IL-6 and TGF-β signals in relapsed MS patients peripheral blood.

    abstract:OBJECTIVE:Effects of therapeutic plasma exchange (TPE) on immune cells and their cytokine production in MS, are unknown. Since interleukine-6 and tumor growth factor-β have critical roles in MS immunopathogenesis, the impacts of TPE on the expression of these cytokines and their receptors on the surface of CD4+ T lymph...

    journal_title:Journal of clinical apheresis

    pub_type: 杂志文章


    authors: Jamshidian A,Abd-Nikfarjam B,Khademi Z,Shaygannejad V,Salehi M

    更新日期:2020-04-01 00:00:00