Rapid detection of high-risk Enterococcus faecium clones by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.


:We aimed to explore the potential of matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for early identification of dominant Enterococcus faecium (Efm) clones involved in human infections. Well-characterized Efm isolates (n=77), analyzed by pulsed-field gel electrophoresis and multilocus sequence typing(eBURST and BAPS [Bayesian analysis of population structure] algorithms), and belonging to different hospital (n=53) and community (n=24) phylogenomic groups, were tested. Mass spectra (Bruker) were analyzed by visual inspection and different chemometric tools. Discrimination between groups comprising isolates commonly found in hospitals (BAPS 2.1a, 3.3a1, 3.3a2) and community (BAPS 2.1b and 3.2) was achieved with >99% accuracy, while identification of sequence types belonging to different BAPS subgroups was associated with >95% correct predictions. Our work is a proof of concept with regard to the suitability of MALDI-TOF MS in the identification of high-risk Efm clones. Further studies including strains from a wider variety of clones and sources will strengthen the potential of the workflow here described.


Freitas AR,Sousa C,Novais C,Silva L,Ramos H,Coque TM,Lopes J,Peixe L




Has Abstract


2017-04-01 00:00:00














  • Characterization of extended-spectrum beta-lactamases, antimicrobial resistance genes, and plasmid content in Escherichia coli isolates from different sources in Rio de Janeiro, Brazil.

    abstract::We analyse the diversity of extended-spectrum beta-lactamases (ESBLs), antibiotic resistance genes, and plasmid content among Escherichia coli from a community and a hospital setting in Rio de Janeiro, Brazil. ESBL producers (CTX-M-2, CTX-M-9, CTX-M-59) belonged to different phylogroups/sequence types, and bla(CTX-M) ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Queiroz ML,Antunes P,Mourão J,Merquior VL,Machado E,Peixe LV

    更新日期:2012-09-01 00:00:00

  • Pharmacodynamics of 750 mg and 500 mg doses of levofloxacin against ciprofloxacin-resistant strains of Streptococcus pneumoniae.

    abstract::An in vitro pharmacokinetic model (IVPM) was used to evaluate the pharmacodynamics of the 750 mg and 500 mg doses of levofloxacin against 4 ciprofloxacin-nonsusceptible Streptococcus pneumoniae. Levofloxacin MICs ranged from 1.4 to 3.2 micro g/ml. Log-phase cultures (5 x 10(7) cfu/ml) were inoculated into the IVPM and...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Lister PD

    更新日期:2002-09-01 00:00:00

  • Evaluation of antimicrobial susceptibility for beta-lactams using the Etest method against clinical isolates from 100 medical centers in Japan (2006).

    abstract::This antimicrobial resistance surveillance study was performed in 100 medical centers. Susceptibility testing (Etest; AB BIODISK, Solna, Sweden) of 9152 strains including Escherichia coli (991 strains), Klebsiella spp. (1000 strains), Enterobacter spp. (971 strains), Citrobacter spp. (803 strains), indole-positive Pro...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Ishii Y,Tateda K,Yamaguchi K,Japan Antimicrobial Resistance Surveillance Participants Group (JARS).

    更新日期:2008-02-01 00:00:00

  • Identification by 16S ribosomal RNA gene sequencing of an Enterobacteriaceae species with ambiguous biochemical profile from a renal transplant recipient.

    abstract::Traditional ways of identification of bacteria by phenotypic characteristics cannot be used for non-cultivable organisms and organisms with unusual biochemical profiles. In this study, an Enterobacteriaceae was isolated in pure growth from the mid-stream urine of a 67-year old renal transplant recipient with urinary t...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Woo PC,Cheung EY,Leung K,Yuen K

    更新日期:2001-02-01 00:00:00

  • Varicella associated intracerebral hemorrhage in the absence of thrombocytopenia.

    abstract::A young woman with varicella complicated by streptococcal cellulitis developed an intracerebral bleed in the absence of thrombocytopenia, serious coagulopathy, or a detectable vascular lesion. We postulate that during the acute infection with varicella, a cerebral endothelial lesion was produced which later caused the...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Fikrig E,Barg NL

    更新日期:1989-07-01 00:00:00

  • Distribution of capsular and surface polysaccharide serotypes of Staphylococcus aureus.

    abstract::Because of its ability to cause serious and fatal infections, Staphylococcus aureus remains one of the most feared microorganisms. Methicillin-resistant S. aureus (MRSA) has long been a common pathogen in healthcare facilities, but within the past decade, it has emerged as a problematic pathogen in the community setti...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: von Eiff C,Taylor KL,Mellmann A,Fattom AI,Friedrich AW,Peters G,Becker K

    更新日期:2007-07-01 00:00:00

  • Phaeomycotic cysts caused by Phoma species.

    abstract::Phoma species are primarily phytopathogens which have been reported to sporadically cause human disease. We report a patient with phaeohyphomycotic cysts caused by Phoma species, which were initially mistaken for ganglions. ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Vasoo S,Yong LK,Sultania-Dudani P,Scorza ML,Sekosan M,Beavis KG,Huhn GD

    更新日期:2011-08-01 00:00:00

  • Cephalothin is not a reliable surrogate marker for oral cephalosporins in susceptibility testing of Enterobacteriaceae causing urinary tract infection.

    abstract::Vitek® 2 (bioMérieux) is a widely used commercial antimicrobial susceptibility test (AST) system. AST-N244 card includes cephalothin as first-generation cephalosporin. We compared the cephalothin susceptibility results obtained with Vitek® 2 AST-N244 to those obtained by broth microdilution (BMD) and disk diffusion (D...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: López IA,Montes JC,Álvarez MJ,Mazarrasa CF,Martínez-Martínez L

    更新日期:2016-12-01 00:00:00

  • Natural antimicrobial susceptibility patterns and biochemical identification of Escherichia albertii and Hafnia alvei strains.

    abstract::Bangladeshi diarrheagenic Hafnia alvei-like strains have been described recently as the new species Escherichia albertii (Int J Syst Evolut Microbiol. 2003;53:807-810). The natural susceptibility of 21 E. albertii and 76 H. alvei strains to 69 antimicrobial agents was examined, applying a microdilution procedure in Is...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Stock I,Rahman M,Sherwood KJ,Wiedemann B

    更新日期:2005-03-01 00:00:00

  • Use of probes and amplification techniques for the diagnosis and prognosis of human immunodeficiency virus (HIV-1) infections.

    abstract::The recent development of nucleic acid amplification methodologies has markedly improved our ability to detect very low levels of specific nucleic acids. Amplification techniques have been combined with product detection systems that are designed for high throughput and are automatable. These developments are drastica...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审


    authors: Arens M

    更新日期:1993-02-01 00:00:00

  • Use of and cost savings with morphologic criteria and the spot indole test as a routine means of identification of Escherichia coli.

    abstract::The use of primary isolation plate colonial morphologic criteria (CMC) of a flat, nonmucoid, lactose-fermenting, gram-negative rod on MacConkey agar and the spot indole (SI) test from the sheep blood agar plate was evaluated as a means for identification of Escherichia coli in comparison to kit (Micro-ID, API-20E) and...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Bale MJ,McLaws SM,Fenn JP,Matsen JM

    更新日期:1984-06-01 00:00:00

  • Urine D-arabinitol/L-arabinitol ratio in diagnosing Candida infection in patients with haematological malignancy and HIV infection.

    abstract::Adult patients with hematologic malignancies along with HIV infected patients were prospectively studied to determine the performance of urine D-arabinitol/L-arabinitol (DA/LA) ratio in diagnosing invasive candidiasis. Ten evaluable febrile neutropenic patients had proven invasive candidiasis and elevated DA/LA ratios...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Eisen DP,Bartley PB,Hope W,Sigmundsdottir G,Pehrson C,Larsson L,Christensson B

    更新日期:2002-01-01 00:00:00

  • More potency assay results for generic non-USA lots of piperacillin/tazobactam and initial reports for generic meropenem compounds marketed in the USA.

    abstract::An ongoing program of international generic antimicrobial potency assays for piperacillin/tazobactam has been summarized here through December 2010, and the initial results for meropenem generic lots from the United States are also presented. Fifteen additional piperacillin/tazobactam generic lots revealed an average ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Jones RN,Sader HS,Flamm RK,Watters AA

    更新日期:2013-05-01 00:00:00

  • Epidemiologic typing of multiply drug-resistant Pseudomonas aeruginosa isolated from an outbreak in an intensive care unit.

    abstract::From June to August 1991, there was an outbreak of Pseudomonas aeruginosa infections in an intensive care unit in a general hospital in Sao Paulo, Brazil. We obtained 14 isolates from 14 patients, 11 from tracheal aspirate, and 3 from surgical wound exudates. These strains were typed by serotyping, pyocin typing, and ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Sader HS,Pignatari AC,Leme IL,Burattini MN,Tancresi R,Hollis RJ,Jones RN

    更新日期:1993-07-01 00:00:00

  • Analytical and clinical evaluation of a novel assay for anti-HBc IgG measurement in serum of subjects with overt and occult HBV infection.

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    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Caviglia GP,Olivero A,Ciancio A,Tandoi F,Troshina G,Rosso C,Abate ML,Younes R,Ribaldone DG,Smedile A,Rizzetto M,Romagnoli R,Saracco GM,Bugianesi E

    更新日期:2020-04-01 00:00:00

  • Effectual detection of group B streptococci with reduced penicillin susceptibility (PRGBS) by commercially available methicillin-resistant-Staphylococcus aureus (MRSA)-selective agar.

    abstract::We evaluated the feasibility and efficacy of a commercially available methicillin-resistant Staphylococcus aureus (MRSA)-selective agar, chromID(™) MRSA, to detect group B streptococci with reduced penicillin susceptibility (PRGBS) in this study. The results showed 72.4% (21/29) sensitivity and 98.4% (60/61) specifici...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Fukigai S,Morimoto M,Kimura K,Doyama Y,Miyazaki A,Kamiya C,Banno H,Morishima E,Onoda T,Nagano N,Jin W,Wachino JI,Yamada K,Arakawa Y

    更新日期:2016-07-01 00:00:00

  • Pyrosequencing of a recA gene variable region for Burkholderia cepacia complex genomovar identification.

    abstract::We developed an assay to identify Burkholderia cepacia complex genomovars by Pyrosequencing of a variable recA gene segment. Fifteen reference strains and 30 clinical isolates of B. cepacia complex were sequenced. Full 77-base pair target sequences were obtained from 44 of the 45 isolates, and BLAST queries of the seq...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Slinger R,Yan L,Myers R,Ramotar K,St Denis M,Aaron SD

    更新日期:2007-08-01 00:00:00

  • Bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections: direct molecular versus culture-based detection.

    abstract::Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. Real-time PCR resulted in an increased detection rate of 12% for B. fragilis and could improve the detection of B. fragilis in clinical samples. ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Stappers MH,Hagen F,Reimnitz P,Mouton JW,Meis JF,Gyssens IC

    更新日期:2016-06-01 00:00:00

  • Bacteriological studies with ampicillin/sulbactam on selected strains of gram-negative bacteria.

    abstract::Antibacterial activity of sulbactam or ampicillin alone and in combination on ampicillin-resistant, beta-lactamase-producing Gram-negative bacteria (Citrobacter freundii and Escherichia coli) was studied. Inhibition of beta-lactamase activity by sulbactam was investigated using intact and disrupted cells. Minimal inhi...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Bello H,del Solar E,González C,Garcia A,Urrea R,Zemelman R

    更新日期:1988-10-01 00:00:00

  • Antimicrobial resistance of Clostridium difficile isolates in a tertiary medical center, Israel.

    abstract::The antimicrobial susceptibilities of 49 Clostridium difficile isolates obtained from patients with C. difficile-associated diarrhea to metronidazole, vancomycin, rifampicin, fusidic acid, doxycycline, and linezolid were determined by the disc diffusion and Etest (Biodisk, Solna, Sweden). Random amplification of polym...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Bishara J,Bloch Y,Garty M,Behor J,Samra Z

    更新日期:2006-02-01 00:00:00

  • Evaluation of a sequential enzyme immunoassay testing algorithm for Lyme disease demonstrates lack of test independence but high diagnostic specificity.

    abstract::To diagnose Lyme disease, a two-tier testing algorithm is used in which supplemental IgM and IgG immunoblots to detect antibody to Borrelia burgdorferi are reflexively performed if a first-tier assay, such as a whole-cell sonicate-based enzyme immunoassay (WCS EIA), is reactive. Recent data suggest that equal specific...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Wormser GP,Molins CR,Levin A,Lipsett SC,Nigrovic LE,Schriefer ME,Branda JA

    更新日期:2018-07-01 00:00:00

  • The current and future impact of antimicrobial resistance among nosocomial bacterial pathogens.

    abstract::The selection of drug-resistant microorganisms has generally been associated with the widespread use of antimicrobial agents. The emergence of these antimicrobial resistance has an undesirable impact that often severely limits the use of these drugs that could have otherwise been identified as "drugs of first choice."...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审


    authors: Jones RN

    更新日期:1992-02-01 00:00:00

  • Evaluation of the Abbott ARCHITECT Toxo IgM assay.

    abstract::Development of the ARCHITECT Toxo IgM assay has been done to assist the clinician in acute Toxoplasma gondii infection detection, especially in pregnant women. Its use, in conjunction with ARCHITECT Toxo IgG and Toxo Avidity assays, will provide an array of assays particularly useful in the monitoring of pregnant fema...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Sickinger E,Braun HB,Praast G,Stieler M,Gundlach C,Birkenbach C,Prostko J,Palafox MA,Frias E,Hsu S,Matias M,Pucci D,Hausmann M,Sagel U,Smith D

    更新日期:2009-07-01 00:00:00

  • Early cytokine and antibody responses against Coxiella burnetii in aerosol infection of BALB/c mice.

    abstract::Coxiella burnetii, a Gram-negative intracellular bacterium, can give rise to Q fever in humans and is transmitted mainly by inhalation of infected aerosols from animal reservoirs. Serology is commonly used to diagnose Q fever, but the early cellular immune response-i.e., C. burnetii-specific interferon γ (IFN-γ) produ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Schoffelen T,Self JS,Fitzpatrick KA,Netea MG,van Deuren M,Joosten LA,Kersh GJ

    更新日期:2015-04-01 00:00:00

  • Prevalence of beta-lactam nonsusceptible Gram-negative bacilli and use and interpretation of current susceptibility breakpoints: a survey of infectious disease physicians.

    abstract::Beta-lactam-resistant Enterobacteriaceae represent an important public health problem; however, questions exist about their prevalence and the impact of recent breakpoint changes on clinical practice. We surveyed infectious disease physicians to better understand these issues. Many reported encountering resistant Ente...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Kallen AJ,Beekmann SE,Limbago B,Lentnek AL,Polgreen PM,Patel J,Srinivasan A

    更新日期:2011-11-01 00:00:00

  • Successful use of culture and enrichment for the detection of OXA-181-producing Escherichia coli from rectal swab samples falsely categorized as negative by Xpert® Carba-R.

    abstract::An OXA-181 producing Escherichia coli isolate that went recurrently undetected directly from rectal swab sample using Xpert® Carba-R, was successfully detected using ertapenem supplemented broth enrichment followed by culture-based method. Our data suggest that implementation of culture-based methods plus enrichment m...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Girlich D,Ouzani S,Langlois I,Begasse C,Arangia N,Fortineau N,Naas T,Dortet L

    更新日期:2020-01-01 00:00:00

  • Discrepancies between in vitro activity of and in vivo response to antimicrobial agents.

    abstract::The correlation between in vitro antimicrobial activity and the in vivo response to antimicrobial therapy is affected by multiple host factors, the site and nature of the infection, and the pharmacokinetics of the antimicrobial and its penetration into areas of infection. In certain instances, discrepancies are also c...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Washington JA 2nd

    更新日期:1983-03-01 00:00:00

  • Mycobacterium wolinskyi: a case series and review of the literature.

    abstract::Mycobacterium wolinskyi is an uncommonly encountered rapidly growing mycobacterium. To date, only 12 clinical cases have been reported in the literature. In this report, we describe 5 additional cases of M. wolinskyi infection seen at the Mayo Clinic in Rochester, MN, since 2009. The clinical manifestations were stern...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审


    authors: Ariza-Heredia EJ,Dababneh AS,Wilhelm MP,Wengenack NL,Razonable RR,Wilson JW

    更新日期:2011-12-01 00:00:00

  • The outcome of candiduria in pediatric patients.

    abstract::The presence of Candida in the urine of a seriously ill, pediatric patient presents a management problem because of a lack of information concerning the natural history of candiduria and its relationship to disseminated candidiasis. In this retrospective study, the outcome of candiduria was examined in a group of 54 p...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Chun CS,Turner RB

    更新日期:1991-03-01 00:00:00

  • Major methodology-dependent discordant susceptibility results for Bacteroides fragilis group isolates but not other anaerobes.

    abstract::Two standardized susceptibility test methods, a broth microdilution (BMD) and agar dilution (AD) method were performed on a total of 441 clinical isolates of anaerobes with ceftizoxime, cefotaxime, ceftriaxone, cefoxitin, piperacillin, and metronidazole. Against the 339 strains of the Bacteroides fragilis group BMD mi...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Aldridge KE,Schiro DD

    更新日期:1994-11-01 00:00:00