Chlorination induced damage and recovery in marine diatoms: Assay by SYTOX® Green staining.

Abstract:

:Phytoplankton entrained into cooling water systems of coastal power stations are subjected to acute chemical stress due to biocides (chlorine) used for biofouling control. They are subsequently released into the environment, where they may survive/recover or succumb. Experiments were conducted to evaluate the susceptibility of a centric (Chaetoceros lorenzianus) and pennate (Navicula sp.) diatom to in-plant administered concentrations of chlorine (0.2-0.5mg/L, TRO). Viability of cells exposed to chlorine was assessed by SYTOX® Green fluorimetry and was compared with other conventional end points like total cell counts, chlorophyll a content and cellular autofluorescence. Results showed a concentration-dependant reduction in viability, chlorophyll a and autofluorescence. C. lorenzianus cells were more susceptible to chlorine compared to Navicula sp. SYTOX® Green staining appears to be a sensitive method to assess chlorine-induced damages. The data show that in-use levels of chlorination can potentially impact entrained organisms; however, they can recover when returned to coastal waters.

journal_name

Mar Pollut Bull

authors

Venkatnarayanan S,Sriyutha Murthy P,Nancharaiah YV,Kirubagaran R,Venugopalan VP

doi

10.1016/j.marpolbul.2016.12.059

subject

Has Abstract

pub_date

2017-11-30 00:00:00

pages

819-826

issue

2

eissn

0025-326X

issn

1879-3363

pii

S0025-326X(16)31063-3

journal_volume

124

pub_type

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