Development of a Time and Cost Benefit Antibody Binding Test-Based Method for Determination of Rabies Vaccine Potency.


:This study is an improvement on the antibody binding test, known as ABT method, to develop a simple and fast method in comparison with NIH for determination of rabies vaccine potency. In the current study, several commercial human and veterinary vaccines were tested using both modified ABT and NIH methods. The ED50 was calculated using the probit method and the relative potency of each vaccine was measured based on the reference vaccine. The test was repeated four times to calculate the reproducibility of the method. Statistical analysis indicated that there was no significant difference between the result obtained from NIH and modified ABT method for either human or veterinary vaccines (p > 0.05). In addition, the linearity of the method (R2) was calculated as 0.94 by serial dilution of a test vaccine. Coefficient variances were determined as less than and more than 10% for the human and veterinary rabies vaccines, respectively. In conclusion, the findings suggest that the modified method could be considered as an alternative approach for rabies vaccine potency determination in in-process quality control tests at industrial scale. It is a time and cost benefit method and accuracy may further be increased by employing monoclonal antibodies against trimeric form of G glycoprotein. However, the use of serum samples may be useful compared with an artificial mix of antibodies because other components from the serum samples could have a positive impact on cell sensitivity and mimic more the complexity of the immune response. Although the modified test has solved a fundamental problem, it is still not sensitive enough for veterinary vaccine assessment and needs further modifications to obtain the acceptability criteria.


Viral Immunol


Viral immunology


Asgary V,Mojtabavi N,Janani A,Mousavi T,Hadjati J,Khosravy MS,Ahangari Cohan R




Has Abstract


2017-04-01 00:00:00












  • Induction of cytotoxic T lymphocytes of heterogeneous specificities by immunization with a single peptide derived from influenza A virus.

    abstract::We examined whether immunization with a single peptide induces cytotoxic T lymphocytes (CTLs) of heterogeneous specificities in vivo. Immunization of BALB/c mice with the peptide H2:529-537, which corresponded to amino acid residues 529-537 on the HA2 subunit transmembrane region of influenza A/Jap virus (H2N2) and po...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Masaki H,Tamura M,Kurane I

    更新日期:2000-01-01 00:00:00

  • Characterization of Anti-HCV Antibodies in IL-10-Treated Patients.

    abstract::There is limited information on the direct role of the neutralizing antibody responses against hepatitis C virus (HCV) infection or methodologies to study them. Previously we have demonstrated that interleukin-10 (IL-10) administered to chronic hepatitis patients led to a decrease in disease activity, but an increase ...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Eksioglu EA,Bess J,Jones G,Dettloff J,Dangmeon P,Dong HJ,Zhu H,Firpi R,Xu Y,Nelson DR,Liu C

    更新日期:2010-08-01 00:00:00

  • Induction of immunity in the respiratory tract and protection from bovine herpesvirus type 1 infection by different routes of immunization with recombinant adenovirus.

    abstract::To investigate the capability of different routes of immunization with replication-competent recombinant adenovirus to induce antigen-specific antibody responses, we immunized cotton rats with a human adenovirus type 5 (HAd5) vector expressing the glycoprotein D (gD) of bovine herpesvirus type 1 (BHV-1) (gD-dE3). Diff...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Papp Z,Babiuk LA,Baca-Estrada ME

    更新日期:1998-01-01 00:00:00

  • Antibodies against human papillomavirus type 16 and 18 E6 and E7 proteins in cervicovaginal washings and serum of patients with cervical neoplasia.

    abstract::Serum antibodies against the E6 and E7 proteins of human papillomavirus (HPV) 16 and 18 are associated with cervical cancer. The aim of this study was to investigate the presence of local antibodies against HPV in cervicovaginal washings (CWs). In this study antibodies against the native HPV16 and HPV18 E6/E7 proteins...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Tjiong MY,Zumbach K,Schegget JT,van der Vange N,Out TA,Pawlita M,Struyk L

    更新日期:2001-01-01 00:00:00

  • Characterization of "modified-self"-induced specific antibody hyporesponsiveness to herpes simplex virus.

    abstract::Pretreatment of mice iv with syngeneic spleen cells modified with soluble HSV envelope antigens induced an anti-HSV antibody hyporesponsiveness following challenge with infectious virus. The epitope density on the HSV-modified spleen cells was quantitated using a photon-counting spectrofluorimeter so that observed imm...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Wilson LA,Thompson JJ,Ohkawa S,Scott DW

    更新日期:1992-07-01 00:00:00

  • Severe acute respiratory syndrome, a pathological immune response to the new coronavirus--implications for understanding of pathogenesis, therapy, design of vaccines, and epidemiology.

    abstract::Findings coming from autopsies and serum of SARS patients suggest an important immune-inflammatory implication in the evolution to respiratory distress. Conditions such as HIV infection or treatment with immunosuppressors (in cancer or autoimmune diseases) are not among the bad prognosis factors for development of dis...

    journal_title:Viral immunology

    pub_type: 杂志文章,评审


    authors: Bermejo JF,Muñoz-Fernandez MA

    更新日期:2004-01-01 00:00:00

  • Evidence for Increased Inflammatory Cytokine Profile in Hepatitis E Virus-Infected Obese Patients: Implications for Chronic Liver Disease.

    abstract::We aimed to characterize the contribution of hepatitis E virus (HEV) in perpetuating the cytokine-mediated inflammatory setting related to liver damage in the context of obesity. Herein, serum samples from patients with liver disease were retrospectively analyzed and categorized as normal-weight patients (NW), overwei...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Viera-Segura O,Panduro A,Trujillo-Ochoa JL,Copado-Villagrana E,Torres-Valadez R,Sepulveda-Villegas M,Roman S,Fierro NA

    更新日期:2020-11-01 00:00:00

  • Active and Persistent Cytomegalovirus Infections Affect T Cells in Young Adult HIV Patients Commencing Antiretroviral Therapy.

    abstract::Altered T cell profiles have been linked with metrics of persistent cytomegalovirus (CMV) infections in healthy aging and older HIV patients stable on antiretroviral therapy (ART). In this study, we use CMV DNA to identify active infections, and levels of CMV-reactive antibody to assess the persistent burden of CMV in...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Ariyanto IA,Estiasari R,Waters S,Wulandari EAT,Fernandez S,Lee S,Price P

    更新日期:2018-07-01 00:00:00

  • Environmental toxins as modulators of antiviral immune responses.

    abstract::Exposure to environmental contaminants has a profound effect on immune function, yet mechanistic understanding of how pollutants deregulate immune responses has, for many chemicals, remained elusive. Available data suggest that certain pollutants alter host immune responses and increase susceptibility to viral infecti...

    journal_title:Viral immunology

    pub_type: 杂志文章,评审


    authors: Lawrence BP

    更新日期:2007-07-01 00:00:00

  • Detection of genetic diversity among bovine immunodeficiency virus population by single-strand conformation polymorphism analysis.

    abstract::Serial virus specimens rescued from rabbits, experimentally infected with bovine immunodeficiency (BIV) strain R29, were monitored for changes in quasispecies population, using the single-strand conformation polymorphism (SSCP) analysis. The generation of characteristic SSCP patterns enables the rapid differentiation ...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Kalvatchev Z,Walder R,Garzaro D,Barrios M

    更新日期:2000-01-01 00:00:00

  • Induction of protective immunity against herpes simplex virus with DNA encoding the immediate early protein ICP 27.

    abstract::Using a mouse zosteriform model that mimics human herpes simplex virus (HSV) infection in several aspects, the effectiveness of plasmid DNA encoding the immediate early protein ICP 27 was evaluated as a vaccine. Animals were immunized intramuscularly twice with DNA, then either challenged with virus or killed, and the...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Manickan E,Yu Z,Rouse RJ,Wire WS,Rouse BT

    更新日期:1995-01-01 00:00:00

  • Evaluation of the blood stabilizers TransFix and Cyto-Chex BCT for low-cost CD4 T-cell methodologies.

    abstract::TransFix(TM) and Cyto-Chex((R)) BCT (blood collection tube) reagents have been shown to maintain whole blood integrity for delayed immunophenotyping by flow cytometry. We evaluated the ability of these blood-stabilizing reagents to preserve HIV-seropositive blood for delayed CD4(+) T-cell quantification utilizing the ...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Plate MM,Louzao R,Steele PM,Greengrass V,Morris LM,Lewis J,Barnett D,Warrino D,Hearps AC,Denny T,Crowe SM

    更新日期:2009-10-01 00:00:00

  • ORF3 of Hepatitis E Virus Inhibits the Expression of Proinflammatory Cytokines and Chemotactic Factors in LPS-Stimulated Human PMA-THP1 Cells by Inhibiting NF-κB Pathway.

    abstract::Hepatitis E virus (HEV) is one of the primary causative agents of acute hepatitis. It is noteworthy that HEV can develop chronic infection and even lead to liver cirrhosis; however, the mechanism has not been revealed. In this study, the ELISA assay was used to detect protein levels, and we found that HEV open reading...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Lei Q,Li L,Cai J,Huang W,Qin B,Zhang S

    更新日期:2016-03-01 00:00:00

  • Unique Infectious Strategy of H5N1 Avian Influenza Virus Is Governed by the Acid-Destabilized Property of Hemagglutinin.

    abstract::Highly pathogenic avian influenza (HPAI) H5N1 virus emerged in 1997 as a zoonotic disease in Hong Kong. It has since spread to Asia and Europe and is a serious threat to both the poultry industry and human health. For effective surveillance and possible prevention/control of HPAI H5N1 viruses, it is necessary to under...

    journal_title:Viral immunology

    pub_type: 杂志文章,评审


    authors: Daidoji T,Watanabe Y,Arai Y,Kajikawa J,Hirose R,Nakaya T

    更新日期:2017-07-01 00:00:00

  • A truncated plasmid-encoded HIV-1 reverse transcriptase displays strong immunogenicity.

    abstract::Besides being an important target in the antiretroviral therapy against the human immunodeficiency virus type 1 (HIV-1), the HIV-1 reverse transcriptase (RT) enzyme has potential as a vaccine antigen. In this study, we explored the ability of plasmid-encoded RT to induce cell-mediated immune responses. The strategy fo...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Hallengärd D,Wahren B,Bråve A

    更新日期:2013-04-01 00:00:00

  • Correlation of clinical parameters and immunological function with human immunodeficiency virus plasma viremia in children.

    abstract::Studies of immune function in human immunodeficiency virus (HIV)-infected children are important, because functional abnormalities can precede CD4+ T-cell loss and are associated with the development of opportunistic and bacterial infections. We sought to correlate clinical parameters and immunological function with H...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Peters VB,Mayer L,Sperber KE

    更新日期:1999-01-01 00:00:00

  • Replicon Particle Expressing the E2 Glycoprotein of Bovine Viral Diarrhea Virus Immunization and Evaluation of Antibody Response.

    abstract::The objective of this study was to develop a new antigen delivery system using an alphavirus replicon particle (RP) to induce humoral antibody responses against bovine viral diarrhea virus (BVDV) recombinant antigen produced from envelope glycoprotein E2. An alphavirus RP expressing the E2 glycoprotein of BVDV was use...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Hossain MM,Rowland RR

    更新日期:2018-01-01 00:00:00

  • Impact of immunizations with porcine reproductive and respiratory syndrome virus on lymphoproliferative recall responses of CD8+ T cells.

    abstract::The objective of this study was to investigate the immune responses elicited by either a modified-live (MLV) or a killed virus (KV) porcine reproductive and respiratory syndrome virus (PRRSV) vaccine. Specifically, we investigated the effects of multiple vaccinations on antigen-specific cellular and antibody responses...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Bassaganya-Riera J,Thacker BJ,Yu S,Strait E,Wannemuehler MJ,Thacker EL

    更新日期:2004-01-01 00:00:00

  • Cytokine expression in the cervical stroma of HIV-positive and HIV-negative women with cervical intraepithelial neoplasia.

    abstract::Cervical intraepithelial neoplasias (CINs) are a major public health issue. The prevalence of CINs is higher in women with the human immunodeficiency virus (HIV). The objective of this study was to determine whether there are differences in the immune responses in the cervical stroma of HIV-infected and -uninfected wo...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Guimarães MV,Michelin MA,Lucena AA,Lodi CT,Lima MI,Murta EF,Melo VH

    更新日期:2014-09-01 00:00:00

  • Impaired mitogen-driven proliferation and cytokine transcription of lymphocytes from macaques early after simian immunodeficiency virus (SIV) infection.

    abstract::Altered cytokine transcription might play an important role in the pathogenesis of human immunodeficiency virus (HIV) infection in humans. The infection of rhesus macaques with simian immunodeficiency virus (SIV) provides a relevant animal model for HIV infection. Therefore, we evaluated the cyokine transcription of p...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Spring M,Bodemer W,Stahl-Hennig C,Nisslein T,Hunsmann G,Dittmer U

    更新日期:1997-01-01 00:00:00

  • HIV-1 matrix protein p17 prevents loss of CD28 expression during IL-2-induced maturation of naïve CD8(+) T cells.

    abstract::Naïve CD8(+) T cells differentiate into effectors secreting various cytokines that modulate immune functions. A striking finding for most HIV-1-infected patients is that they accumulate CD8(+) T cells belonging to early and intermediate differentiated elements. Structural HIV-1 proteins, and among these the matrix pro...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Avolio M,Caracciolo S,Tosti G,Vollero L,Fiorentini S,Caruso A

    更新日期:2008-06-01 00:00:00

  • Autoantibody idiotypy and neonatal B cell repertoire.

    abstract::During ontogeny, antibody variable (V) regions are subjected to selection events at the level of B-cell clones bearing on their surfaces Ig molecules useful to the developing organism. Antigenic determinants of immunoglobulin V regions (idiotypes) are believed to play an essential role in molecular recognition and imm...

    journal_title:Viral immunology

    pub_type: 杂志文章,评审


    authors: Sollazzo M,Glotz D,Castiglia D,Zanetti M

    更新日期:1989-01-01 00:00:00

  • Differences in clinical disease and immune response of pigs challenged with a high-dose versus low-dose inoculum of porcine reproductive and respiratory syndrome virus.

    abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be an economically important infectious disease of swine. Mechanisms governing activation of the innate immune response to PRRSV remain to be elucidated. Virulence differences observed between PRRSV isolates have been attributed to replication ab...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Loving CL,Brockmeier SL,Vincent AL,Lager KM,Sacco RE

    更新日期:2008-09-01 00:00:00

  • Vaccine-associated enhanced respiratory disease does not interfere with the adaptive immune response following challenge with pandemic A/H1N1 2009.

    abstract::The implications of sequential prime and challenge with mismatched influenza A viruses is a concern in mammals, including humans. We evaluated the ability of pigs affected with vaccine-associated enhanced respiratory disease (VAERD) to generate a humoral immune response against the heterologous challenge virus incitin...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Gauger PC,Loving CL,Lager KM,Janke BH,Kehrli ME Jr,Roth JA,Vincent AL

    更新日期:2013-10-01 00:00:00

  • Mannan-binding lectin (MBL) serum concentration in relation to propagation of infectious bronchitis virus (IBV) in chickens.

    abstract::Mannan-binding lectin (MBL) is a collectin that mediates activation of the complement system and is of importance for host defenses. In humans low concentrations of MBL in serum have been associated with susceptibility to several viral diseases. To understand the function of MBL in relation to infectious viral disease...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Juul-Madsen HR,Norup LR,Handberg KJ,Jørgensen PH

    更新日期:2007-12-01 00:00:00

  • Immunological characterization of respiratory syncytial virus N protein epitopes recognized by human cytotoxic T lymphocytes.

    abstract::Virus-specific cytotoxic T lymphocytes (CTLs) are crucial for the control of respiratory syncytial virus (RSV) infection. This study has identified CTL epitopes of the RSV N protein in healthy subjects. We screened the primary structure of the N protein for HLA-A 0201-binding amino acid consensus motifs, identifying t...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Terrosi C,Di Genova G,Savellini GG,Correale P,Blardi P,Cusi MG

    更新日期:2007-09-01 00:00:00

  • Mapping of B-cell epitopes in the nucleocapsid protein of Puumala hantavirus.

    abstract::Hantavirus nucleocapsid protein (N) has been proven to induce highly protective immune responses in animal models. The knowledge on the mechanisms behind N-induced protection is still limited, although recent data suggest that both cellular and humoral immune responses are of importance. For a detailed B-cell epitope ...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Lundkvist A,Meisel H,Koletzki D,Lankinen H,Cifire F,Geldmacher A,Sibold C,Gött P,Vaheri A,Krüger DH,Ulrich R

    更新日期:2002-01-01 00:00:00

  • Major and minor Kb-restricted epitopes encoded by the endogenous ecotropic murine leukemia virus AKR623 that are recognized by anti-AKR/Gross MuLV CTL.

    abstract::C57BL/6 mice can generate a type-specific and class IH-2Kb-restricted CTL response against histocompatible AKR/Gross murine leukemia virus (MuLV) cell surface antigen positive (GCSA+) tumor cells. These anti-AKR/Gross MuLV CTL are also known to lyse SC.Kb/623 target cells expressing the molecular MuLV clone AKR623 (de...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: White HD,Roeder DA,Lam T,Green WR

    更新日期:1994-01-01 00:00:00

  • Seroprevalence of Cytomegalovirus Infection Among HIV-Infected and HIV-Uninfected Pregnant Women Attending Antenatal Clinic in Harare, Zimbabwe.

    abstract::This study aimed to investigate the seroprevalence of cytomegalovirus (CMV) infection and risk factors associated with CMV acquisition among pregnant women in Zimbabwe. In a cross-sectional study, pregnant women were recruited in late gestation, seeking antenatal care at council clinics in three high-density suburbs i...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Mhandire D,Duri K,Kaba M,Mhandire K,Musarurwa C,Chimusa E,Munjoma P,Mazengera L,Stray-Pedersen B,Dandara C

    更新日期:2019-09-01 00:00:00

  • Antibody-binding epitope differences in the nucleoprotein of avian and mammalian influenza A viruses.

    abstract::Abstract Influenza virus nucleoprotein (NP) binds to the viral genome RNA and forms the internal ribonucleoprotein complex of the virus particle. Avian and human influenza virus NP have characteristic differences at several amino acid positions. It is not known whether any of these differences can be recognized by ant...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Varich NL,Sadykova GK,Prilipov AG,Kochergin-Nikitsky KS,Kushch AA,Masalova OV,Klimova RR,Gitelman AK,Kaverin NV

    更新日期:2011-04-01 00:00:00