Teriparatide attenuates scarring around murine cranial bone allograft via modulation of angiogenesis.


:Nearly all bone fractures in humans can deteriorate into a non-union fracture, often due to formation of fibrotic tissue. Cranial allogeneic bone grafts present a striking example: although seemingly attractive for craniofacial reconstructions, they often fail due to fibrosis at the host-graft junction, which physically prevents the desired bridging of bone between the host and graft and revitalization of the latter. In the present study we show that intermittent treatment with recombinant parathyroid hormone-analogue (teriparatide) modulates neovascularization feeding in the graft surroundings, consequently reducing fibrosis and scar tissue formation and facilitates osteogenesis. Longitudinal inspection of the vascular tree feeding the allograft has revealed that teriparatide induces formation of small-diameter vessels in the 1st week after surgery; by the 2nd week, abundant formation of small-diameter blood vessels was detected in untreated control animals, but far less in teriparatide-treated mice, although in total, more blood capillaries were detected in the animals that were given teriparatide. By that time point we observed expression of the profibrogenic mediator TGF-β in untreated animals, but negligible expression in the teriparatide-treated mice. To evaluate the formation of scar tissue, we utilized a magnetization transfer contrast MRI protocol to differentiate osteoid tissue from scar tissue, based on the characterization of collagen fibers. Using this method we found that significantly more bone matrix was formed in animals given teriparatide than in control animals. Altogether, our findings show how teriparatide diminishes scarring, ultimately leading to superior bone graft integration.






Cohn Yakubovich D,Eliav U,Yalon E,Schary Y,Sheyn D,Cook-Wiens G,Sun S,McKenna CE,Lev S,Binshtok AM,Pelled G,Navon G,Gazit D,Gazit Z




Has Abstract


2017-04-01 00:00:00














  • The effects of local insulin delivery on diabetic fracture healing.

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    pub_type: 杂志文章


    authors: Gandhi A,Beam HA,O'Connor JP,Parsons JR,Lin SS

    更新日期:2005-10-01 00:00:00

  • Short-term immobilization-induced cancellous bone loss is limited to regions undergoing high turnover and/or modeling in mature rats.

    abstract::Estrogen and calcium deficiencies increase both bone resorption and formation, whereas immobilization mainly decreases bone formation. How these functionally different risk factors for bone loss interact in cancellous bone undergoing modeling or remodeling activity is not well understood. Mature (6-month-old) female r...


    pub_type: 杂志文章


    authors: Shen V,Liang XG,Birchman R,Wu DD,Healy D,Lindsay R,Dempster DW

    更新日期:1997-07-01 00:00:00

  • The neuropeptide calcitonin gene-related peptide stimulates insulin-like growth factor I production by primary fetal rat osteoblasts.

    abstract::Calcitonin gene-related peptide (CGRP)-immunoreactive sensory nerve terminals infiltrate all tissues including bone, in which CGRP may play a local regulatory role. To initiate studies on the role of this neuropeptide in bone, osteoblasts were isolated from fetal rat calvariae, treated with CGRP, and analyzed for cAMP...


    pub_type: 杂志文章


    authors: Vignery A,McCarthy TL

    更新日期:1996-04-01 00:00:00

  • AMP-activated protein kinase (AMPK) activity negatively regulates chondrogenic differentiation.

    abstract::Chondrocytes are derived from mesenchymal stem cells, and play an important role in cartilage formation. Sex determining region Y box (Sox) family transcription factors are essential for chondrogenic differentiation, whereas the intracellular signal pathways of Sox activation have not been clearly elucidated. AMP-acti...


    pub_type: 杂志文章


    authors: Bandow K,Kusuyama J,Kakimoto K,Ohnishi T,Matsuguchi T

    更新日期:2015-05-01 00:00:00

  • Delineation of the 1q24.3 microdeletion syndrome provides further evidence for the potential role of non-coding RNAs in regulating the skeletal phenotype.

    abstract::Microdeletions within 1q24 have been associated with growth deficiency, varying intellectual disability, and skeletal abnormalities. The candidate locus responsible for the various phenotypic features of this syndrome has previously been predicted to lie in the area of 1q24.3, but molecular evidence of the causative g...


    pub_type: 杂志文章


    authors: Shepherdson JL,Zheng H,Amarillo IE,McAlinden A,Shinawi M

    更新日期:2021-01-01 00:00:00

  • Bone remodeling and bone matrix quality before and after menopause in healthy women.

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    pub_type: 杂志文章


    authors: Farlay D,Bala Y,Rizzo S,Bare S,Lappe JM,Recker R,Boivin G

    更新日期:2019-11-01 00:00:00

  • Bone geometry and microarchitecture deficits in children with Alagille syndrome.

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    pub_type: 杂志文章


    authors: Kindler JM,Mitchell EL,Piccoli DA,Grimberg A,Leonard MB,Loomes KM,Zemel BS

    更新日期:2020-12-01 00:00:00

  • The relationship between porosity and specific surface in human cortical bone is subject specific.

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    pub_type: 杂志文章


    authors: Lerebours C,Thomas CD,Clement JG,Buenzli PR,Pivonka P

    更新日期:2015-03-01 00:00:00

  • In vivo trabecular microcracks in human vertebral bone.

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    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Wenzel TE,Schaffler MB,Fyhrie DP

    更新日期:1996-08-01 00:00:00

  • Relationships between quantitative histological measurements and noninvasive assessments of bone mass.

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    pub_type: 杂志文章


    authors: Cosman F,Schnitzer MB,McCann PD,Parisien MV,Dempster DW,Lindsay R

    更新日期:1992-01-01 00:00:00

  • High resolution computed tomography of the vertebrae yields accurate information on trabecular distances if processed by 3D fuzzy segmentation approaches.

    abstract:INTRODUCTION:The structure of trabecular bone represents an aspect of bone properties that affects vertebral bone strength independently of bone mineral density [M. Kleerekoper, A. Villanueva, J. Stanciu, D. Rao, and A. Parfitt. The role of three-dimensional trabecular microstructure in the pathogenesis of vertebral co...


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    authors: Krebs A,Graeff C,Frieling I,Kurz B,Timm W,Engelke K,Glüer CC

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  • Prolonged alendronate treatment prevents the decline in serum TGF-β1 levels and reduces cortical bone strength in long-term estrogen deficiency rat model.

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    pub_type: 杂志文章


    authors: Jia J,Yao W,Amugongo S,Shahnazari M,Dai W,Lay YA,Olvera D,Zimmermann EA,Ritchie RO,Li CS,Alliston T,Lane NE

    更新日期:2013-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Wiren KM,Zhang XW,Olson DA,Turner RT,Iwaniec UT

    更新日期:2012-11-01 00:00:00

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    pub_type: 杂志文章


    authors: Saffar JL,Leroux P

    更新日期:1988-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Mora S,Giacomet V,Viganò A,Cafarelli L,Stucchi S,Pivetti V,Manfredini V,Puzzovio M,Zuccotti G

    更新日期:2012-01-01 00:00:00

  • Effect of 1 alpha-hydroxyvitamin D3 on osteoporosis induced by immobilization combined with ovariectomy in rats.

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    pub_type: 杂志文章


    authors: Okumura H,Yamamuro T,Kasai R,Hayashi T,Tada K,Nishii Y

    更新日期:1987-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Kitahama S,Gibson MA,Hatzinikolas G,Hay S,Kuliwaba JL,Evdokiou A,Atkins GJ,Findlay DM

    更新日期:2000-07-01 00:00:00

  • Effect of phosphodiesterase inhibitor-4, rolipram, on new bone formations by recombinant human bone morphogenetic protein-2.

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    authors: Horiuchi H,Saito N,Kinoshita T,Wakabayashi S,Yotsumoto N,Takaoka K

    更新日期:2002-04-01 00:00:00

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    authors: Battaglino RA,Sudhakar S,Lazzari AA,Garshick E,Zafonte R,Morse LR

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  • Impact microindentation assesses subperiosteal bone material properties in humans.

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    authors: Rokidi S,Bravenboer N,Gamsjaeger S,Misof B,Blouin S,Chavassieux P,Klaushofer K,Paschalis E,Papapoulos S,Appelman-Dijkstra N

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  • Ultrastructural localization of matrix vesicles and alkaline phosphatase in the Swarm rat chondrosarcoma: their role in cartilage calcification.

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    pub_type: 杂志文章


    authors: Miller GJ,DeMarzo AM

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  • Intensity-related differences in collagen post-translational modification in MC3T3-E1 osteoblasts after exposure to low- and high-intensity pulsed ultrasound.

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    authors: Saito M,Soshi S,Tanaka T,Fujii K

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    authors: Hernández ER,Revilla M,Seco-Durban C,Villa LF,Cortés J,Rico H

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    pub_type: 杂志文章


    authors: Guillotin B,Bareille R,Bourget C,Bordenave L,Amédée J

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    pub_type: 杂志文章


    authors: Liabeuf S,McCullough K,Young EW,Pisoni R,Zee J,Reichel H,Pecoits-Filho R,Port FK,Stengel B,Csomor PA,Metzger M,Robinson B,Massy ZA

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  • Peroxisome proliferator activated receptor-gamma (PPARgamma) represses thyroid hormone signaling in growth plate chondrocytes.

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    pub_type: 杂志文章


    authors: Wang L,Shao YY,Ballock RT

    更新日期:2005-09-01 00:00:00

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    pub_type: 杂志文章


    authors: Inoue H,Hiraoka K,Hoshino T,Okamoto M,Iwanaga T,Zenmyo M,Shoda T,Aizawa H,Nagata K

    更新日期:2008-06-01 00:00:00

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    pub_type: 杂志文章


    authors: Haimov H,Shimoni E,Brumfeld V,Shemesh M,Varsano N,Addadi L,Weiner S

    更新日期:2020-01-01 00:00:00

  • Toward a unifying theory of bone remodeling.

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    pub_type: 杂志文章


    authors: Martin RB

    更新日期:2000-01-01 00:00:00

  • Microstructural changes of osteopenic trabeculae in the rat.

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    pub_type: 杂志文章


    authors: Yoshida S,Yamamuro T,Okumura H,Takahashi H

    更新日期:1991-01-01 00:00:00