Molecular epidemiology demonstrates that imported and local strains circulated during the 2014 dengue outbreak in Guangzhou, China.


:The dengue virus (DENV) is a vital global public health issue. The 2014 dengue epidemic in Guangzhou, China, caused approximately 40,000 cases of infection and five deaths. We carried out a comprehensive investigation aimed at identifying the transmission sources in this dengue epidemic. To analyze the phylogenetics of the 2014 dengue strains, the envelope (E) gene sequences from 17 viral strains isolated from 168 dengue patient serum samples were sequenced and a phylogenetic tree was reconstructed. All 17 strains were serotype I strains, including 8 genotype I and 9 genotype V strains. Additionally, 6 genotype I strains that were probably introduced to China from Thailand before 2009 were widely transmitted in the 2013 and 2014 epidemics, and they continued to circulate until 2015, with one affinis strain being found in Singapore. The other 2 genotype I strains were introduced from the Malaya Peninsula in 2014. The transmission source of the 9 genotype V strains was from Malaysia in 2014. DENVs of different serotypes and genotypes co-circulated in the 2014 dengue outbreak in Guangzhou. Moreover, not only had DENV been imported to Guangzhou, but it had also been gradually exported, as the viruses exhibited an enzootic transmission cycle in Guangzhou.


Virol Sin


Virologica Sinica


Li G,Pan P,He Q,Kong X,Wu K,Zhang W,Liu Y,Huang H,Liu J,Zhang Z,Wu,Lai X,Liu X,Wu J




Has Abstract


2017-02-01 00:00:00














  • PD1+CCR2+CD8+ T Cells Infiltrate the Central Nervous System during Acute Japanese Encephalitis Virus Infection.

    abstract::Japanese encephalitis (JE) is a viral encephalitis disease caused by Japanese encephalitis virus (JEV) infection. Uncontrolled inflammatory responses in the central nervous system (CNS) are a hallmark of severe JE. Although the CCR2-CCL2 axis is important for monocytes trafficking during JEV infection, little is known...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Zhang F,Qi L,Li T,Li X,Yang D,Cao S,Ye J,Wei B

    更新日期:2019-10-01 00:00:00

  • Visualization of the Oncolytic Alphavirus M1 Life Cycle in Cancer Cells.

    abstract::Oncolytic alphavirus M1 has been shown to selectively target and kill cancer cells, but cytopathic morphologies induced by M1 virus and the life cycle of the M1 strain in cancer cells remain unclear. Here, we study the key stages of M1 virus infection and replication in the M1 virus-sensitive HepG2 liver cancer cell l...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Dan J,Nie L,Jia X,Xu C,Cai J,Lin Y,Hu J,Zhu W,Li Y,Chen D,Liu Y,Hu C,Yan G,Liang J,Zhang Q

    更新日期:2021-01-22 00:00:00

  • Hepatitis C in Laos: A 7-Year Retrospective Study on 1765 Patients.

    abstract::Hepatitis C virus (HCV) is a global health concern, notably in Southeast Asia, and in Laos the presentation of the HCV-induced liver disease is poorly known. Our objective was thus to describe a comprehensive HCV infection pattern in order to guide national health policies. A study on a group of 1765 patients formerly...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Paboriboune P,Vial T,Sitbounlang P,Bertani S,Trépo C,Dény P,Babin FX,Steenkeste N,Pineau P,Deharo E

    更新日期:2018-08-01 00:00:00

  • Trans Complementation of Replication-defective Omsk Hemorrhagic Fever Virus for Antiviral Study.

    abstract::Omsk hemorrhagic fever virus (OHFV) is a tick-borne flavivirus classified as a biosafety level-4 (BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Zhang Q,Li N,Deng C,Zhang Z,Li X,Yoshii K,Ye H,Zhang B

    更新日期:2019-08-01 00:00:00

  • Genetic and Molecular Characterization of H9N2 Avian Influenza Viruses Isolated from Live Poultry Markets in Hubei Province, Central China, 2013-2017.

    abstract::H9N2 subtype avian influenza virus (AIV) is an influenza A virus that is widely spread throughout Asia, where it jeopardizes the poultry industry and provides genetic material for emerging human pathogens. To better understand the epidemicity and genetics of H9 subtype AIVs, we conducted active surveillance in live po...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Hu Z,Peng F,Xiong Z,Zhang W,Li T,Shi Y,Xie J,Jin X,Huang J,Xiao H,Bi D,Song N,Li Z

    更新日期:2020-09-14 00:00:00

  • Different responses of two highly permissive cell lines upon HCV infection.

    abstract::The construction of the first infectious clone JFH-1 speeds up the research on hepatitis C virus (HCV). However, Huh7 cell line was the only highly permissive cell line for HCV infection and only a few clones were fully permissive. In this study, two different fully permissive clones of Huh7 cells, Huh7.5.1 and Huh7-L...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Chen H,Pei R,Chen X

    更新日期:2013-08-01 00:00:00

  • Inference of global HIV-1 sequence patterns and preliminary feature analysis.

    abstract::The epidemiology of HIV-1 varies in different areas of the world, and it is possible that this complexity may leave unique footprints in the viral genome. Thus, we attempted to find significant patterns in global HIV-1 genome sequences. By applying the rule inference algorithm RIPPER (Repeated Incremental Pruning to P...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Wang Y,Rawi R,Hoffmann D,Sun B,Yang R

    更新日期:2013-08-01 00:00:00

  • In vitro anti-influenza virus activities of sulfated polysaccharide fractions from Gracilaria lemaneiformis.

    abstract::In this paper, in vitro anti-influenza virus activities of sulfated polysaccharide fractions from Gracilaria lemaneiformis were investigated. Cytotoxicities and antiviral activities of Gracilaria lemaneiformis polysaccharides (PGL), Gracilaria lemaneiformis polysaccharide fraction-1 (GL-1), Gracilaria lemaneiformis po...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Chen MZ,Xie HG,Yang LW,Liao ZH,Yu J

    更新日期:2010-10-01 00:00:00

  • Identification of immunogenic determinants of the spike protein of SARS-like coronavirus.

    abstract::Bat SARS-like coronavirus (SL-CoV) has a genome organization almost identical to that of SARS-CoV, but the N-terminus of the Spike (S) proteins, which interacts with host receptor and is a major target of neutralizing antibodies against CoVs, of the two viruses has only 63-64% sequence identity. Although there have be...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Zhou P,Han Z,Wang LF,Shi Z

    更新日期:2013-04-01 00:00:00

  • HearNPV Pseudotyped with PIF1, 2, and 3 from MabrNPV: Infectivity and Complex Stability.

    abstract::Effective oral infection is set off by interaction of a group of conserved per os infectivity factors (PIFs) with larval midgut columnar epithelial cells. We constructed pseudotyped viruses by substituting pif1, pif2 or pif3 genes of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) with their homologs from Mamestra...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Makalliwa GA,Wang X,Zhang H,Zhang N,Chen C,Li J,Deng F,Wang H,Wang M,Hu Z

    更新日期:2018-04-01 00:00:00

  • Study on passive immunity: Time of vaccination in kids born to goats vaccinated against Peste des petits ruminants.

    abstract::In this study, the decay of maternal peste des petits ruminants virus (PPRV) antibodies in kids born to goats vaccinated with Asian lineage IV PPR vaccine and the efficacy of passive immunity against PPRV was assessed to determine the appropriate period for vaccination in kids. Serum samples collected from kids born t...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Balamurugan V,Sen A,Venkatesan G,Rajak KK,Bhanuprakash V,Singh RK

    更新日期:2012-08-01 00:00:00

  • The Saffold Virus-Penang 2B and 3C Proteins, but not the L Protein, Induce Apoptosis in HEp-2 and Vero Cells.

    abstract::Our previous work has shown that Saffold virus (SAFV) induced several rodent and primate cell lines to undergo apoptosis (Xu et al. in Emerg Microb Infect 3:1-8, 2014), but the essential viral proteins of SAFV involved in apoptotic activity lack study. In this study, we individually transfected the viral proteins of S...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Xu Y,Victorio CBL,Meng T,Jia Q,Tan YJ,Chua KB

    更新日期:2019-06-01 00:00:00

  • Rapid detection of filoviruses by real-time TaqMan polymerase chain reaction assays.

    abstract::Ebola virus (EBOV) and Marburg virus (MARV) are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. To date, there is no specific laboratory diagnostic test in China, while there is a national need to provid...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Huang Y,Wei H,Wang Y,Shi Z,Raoul H,Yuan Z

    更新日期:2012-10-01 00:00:00

  • Similar neutralizing activity in the HIV-1 infected long term non-progressors(LTNPs) and typical progressors(TPs).

    abstract::Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV-1 infected peoples is still undetermined. In this paper, we compared the protective function of the n...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Wang Z,Li TY,Li JY,Chen LL,Liu YJ,Li HP,Bao ZY,Wang XL,Zhuang DM,Liu SY,Li L

    更新日期:2012-06-01 00:00:00

  • A Crowned Killer's Résumé: Genome, Structure, Receptors, and Origin of SARS-CoV-2.

    abstract::The recent emergence and rapid global spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pose an unprecedented medical and socioeconomic crisis, and the disease caused by it, Coronavirus disease 2019 (COVID-19), was declared a pandemic by the World Health Organization (WHO) on March 11, 2020. C...

    journal_title:Virologica Sinica

    pub_type: 杂志文章,评审


    authors: Wang S,Trilling M,Sutter K,Dittmer U,Lu M,Zheng X,Yang D,Liu J

    更新日期:2020-12-01 00:00:00

  • A Prognostic Model to Predict Recovery of COVID-19 Patients Based on Longitudinal Laboratory Findings.

    abstract::The temporal change patterns of laboratory data may provide insightful clues into the whole course of COVID-19. This study aimed to evaluate longitudinal change patterns of key laboratory tests in patients with COVID-19, and identify independent prognostic factors by examining the associations between laboratory findi...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Tian S,Zhu X,Sun X,Wang J,Zhou Q,Wang C,Chen L,Li S,Xu J

    更新日期:2020-12-01 00:00:00

  • The Prevalence, Genetic Characterization, and Evolutionary Analysis of Porcine Pegivirus in Guangdong, China.

    abstract::Porcine pegivirus (PPgV) is a member of the Pegivirus genus in the Flaviviridae family. PPgV is an emerging virus that has been discovered in swine herds in Germany, the United States, China, Poland, Italy, and the United Kingdom, indicating a wide geographical distribution. In this retrospective study, 339 pig serum ...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Xie Y,Wang X,Feng J,Wei L,Li G,Si G,Chen Y,Yan H,He D

    更新日期:2020-07-08 00:00:00

  • B cell epitopes within VP1 of type O foot-and-mouth disease virus for detection of viral antibodies.

    abstract::In this study, the coding region of type O FMDV capsid protein VP1 and a series of codon optimized DNA sequences coding for VP1 amino acid residues 141-160 (epitope1), tandem repeat 200-213 (epitope2 (+2)) and the combination of two epitopes (epitope1-2) was genetically cloned into the prokaryotic expression vector pP...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Gao SD,Du JZ,Chang HY,Cong GZ,Shao JJ,Lin T,Song S,Xie QG

    更新日期:2010-02-01 00:00:00

  • Isolation and identification of a subgroup A avian leukosis virus from imported meat-type grand-parent chickens.

    abstract::An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced antibody reactions specific to subgroup A or B. But gp85 amino acid sequence comparisons indicated that...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Zhang QC,Zhao DM,Guo HJ,Cui ZZ

    更新日期:2010-04-01 00:00:00

  • Prevalence of HIV Indeterminate Western Blot Tests and Follow-up of HIV Antibody Sero-Conversion in Southeastern China.

    abstract::HIV-indeterminate Western blotting (WB) results are typically obtained in WB confirmatory assays, and the number of indeterminate samples may increase with the detection of HIV infections, which will present considerable challenges for the management of HIV/AIDS. Nucleic acid detection has been used as a laboratory te...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Wu S,Gao M,Zheng J,Yan P,Wang W,Lu X,Qiu Y,Yan Y

    更新日期:2019-08-01 00:00:00

  • Stability of HIV-1 subtype B and C Tat is associated with variation in the carboxyl-terminal region.

    abstract::The multifunctional trans-activator Tat is an essential regulatory protein for HIV-1 replication and is characterized by high sequence diversity. Numerous experimental studies have examined Tat in HIV-1 subtype B, but research on subtype C Tat is lacking, despite the high prevalence of infections caused by subtype C w...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Zhao X,Qian L,Zhou D,Qi D,Liu C,Kong X

    更新日期:2016-06-01 00:00:00

  • Zika virus and Zika fever.

    abstract::An emerging mosquito-borne arbovirus named Zika virus (ZIKV), of the family Flaviviridae and genus Flavivirus, is becoming a global health threat. ZIKV infection was long neglected due to its sporadic nature and mild symptoms. However, recently, with its rapid spread from Asia to the Americas, affecting more than 30 c...

    journal_title:Virologica Sinica

    pub_type: 杂志文章,评审


    authors: Wang Z,Wang P,An J

    更新日期:2016-04-01 00:00:00

  • Foot-and-Mouth Disease Virus Inhibits RIP2 Protein Expression to Promote Viral Replication.

    abstract::Receptors interaction protein 2 (RIP2) is a specific adaptor molecule in the downstream of NOD2. The role of RIP2 during foot-and-mouth disease virus (FMDV) infection remains unknown. Here, our results showed that RIP2 inhibited FMDV replication and played an important role in the activation of IFN-β and NF-ĸB signal ...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Liu H,Xue Q,Zhu Z,Yang F,Cao W,Liu X,Zheng H

    更新日期:2021-01-05 00:00:00

  • Extensive evolution analysis of the global chikungunya virus strains revealed the origination of CHIKV epidemics in Pakistan in 2016.

    abstract::Chikungunya virus (CHIKV) is a mosquito-borne virus that causes epidemics widely in the world especially in the tropical and subtropical regions. Phylogenetic analysis has found that the CHIKV lineages were associated with the spatial and temporal distributions, which were related to the virus adaption to the major mo...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Shi J,Su Z,Fan Z,Wang J,Liu S,Zhang B,Wei H,Jehan S,Jamil N,Shen S,Deng F

    更新日期:2017-12-01 00:00:00

  • Novel SFTSV Phylogeny Reveals New Reassortment Events and Migration Routes.

    abstract::Severe fever with thrombocytopenia syndrome virus (SFTSV), the causative agent of a febrile human disease, was first identified from central and eastern provinces in China, and later in Japan and South Korea. Hubei Province is one of the major SFTS epidemic areas in the central part of China. This study reported the i...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Wu X,Li M,Zhang Y,Liang B,Shi J,Fang Y,Su Z,Li M,Zhang W,Xu L,Wang J,Wu Q,Tang S,Wang H,Zhang T,Peng C,Zheng X,Deng F,Shen S

    更新日期:2020-09-22 00:00:00

  • Establishment of an ELISA to detect Kaposi's sarcoma-associated herpesvirus using recombinant ORF73.

    abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is causally related to Kaposi's sarcoma (KS), primary effusion lymphoma (PEL) and a proportion of cases of multicentric Castleman's disease (MCD). The ORF73 protein was cloned into pQE80L-orf73 and expressed in E.coli and purified. The expressed recombinant ORF73 was iden...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Ouyang XX,Fu BS,Li BL,Zeng Y,Xu FH,Wang LD

    更新日期:2010-06-01 00:00:00

  • Structural and mutational analysis of the interaction between the Middle-East respiratory syndrome coronavirus (MERS-CoV) papain-like protease and human ubiquitin.

    abstract::The papain-like protease (PL(pro)) of Middle-East respiratory syndrome coronavirus (MERS-CoV) has proteolytic, deubiquitinating, and deISGylating activities. The latter two are involved in the suppression of the antiviral innate immune response of the host cell. To contribute to an understanding of this process, we pr...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Lei J,Hilgenfeld R

    更新日期:2016-08-01 00:00:00

  • Novel Primate Bocaparvovirus Species 3 Identified in Wild Macaca Mulatta in China.

    abstract::Primate bocaparvovirus (BOV) is a possible cause of respiratory disorders and gastroenteritis in humans. However, the diversity and evolution of these viruses remain largely unknown, despite the identification of a growing number of BOVs in non-human primates (NHPs). Here, we report the identification of a novel BOV (...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Ao Y,Duan Z

    更新日期:2020-02-01 00:00:00

  • Countrywide Survey for MERS-Coronavirus Antibodies in Dromedaries and Humans in Pakistan.

    abstract::Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as a major reservoir host, the MERS-CoV infection dynamics in camels are not fully understood. Through surveillance in Pakistan, nasal (n = 77...

    journal_title:Virologica Sinica

    pub_type: 杂志文章


    authors: Zohaib A,Saqib M,Athar MA,Chen J,Sial AU,Khan S,Taj Z,Sadia H,Tahir U,Tayyab MH,Qureshi MA,Mansoor MK,Naeem MA,Hu BJ,Khan BA,Ujjan ID,Li B,Zhang W,Luo Y,Zhu Y,Waruhiu C,Khan I,Yang XL,Sajid MS,Corman VM,

    更新日期:2018-10-01 00:00:00

  • Conditionally Reprogrammed Human Normal Airway Epithelial Cells at ALI: A Physiological Model for Emerging Viruses.

    abstract::Cancer cell lines have been used widely in cancer biology, and as biological or functional cell systems in many biomedical research fields. These cells are usually defective for many normal activities or functions due to significant genetic and epigenetic changes. Normal primary cell yields and viability from any orig...

    journal_title:Virologica Sinica

    pub_type: 杂志文章,评审


    authors: Liu X,Wu Y,Rong L

    更新日期:2020-06-01 00:00:00