The golgin coiled-coil proteins capture different types of transport carriers via distinct N-terminal motifs.


BACKGROUND:The internal organization of cells depends on mechanisms to ensure that transport carriers, such as vesicles, fuse only with the correct destination organelle. Several types of proteins have been proposed to confer specificity to this process, and we have recently shown that a set of coiled-coil proteins on the Golgi, called golgins, are able to capture specific classes of carriers when relocated to an ectopic location. RESULTS:Mapping of six different golgins reveals that, in each case, a short 20-50 residue region is necessary and sufficient to capture specific carriers. In all six of GMAP-210, golgin-84, TMF, golgin-97, golgin-245, and GCC88, this region is located at the extreme N-terminus of the protein. The vesicle-capturing regions of GMAP-210, golgin-84, and TMF capture intra-Golgi vesicles and share some sequence features, suggesting that they act in a related, if distinct, manner. In the case of GMAP-210, this shared feature is in addition to a previously characterized "amphipathic lipid-packing sensor" motif that can capture highly curved membranes, with the two motifs being apparently involved in capturing distinct types of vesicles. Of the three GRIP domain golgins that capture endosome-to-Golgi carriers, golgin-97 and golgin-245 share a closely related capture motif, whereas that in GCC88 is distinct, suggesting that it works by a different mechanism and raising the possibility that the three golgins capture different classes of endosome-derived carriers that share many cargos but have distinct features for recognition at the Golgi. CONCLUSIONS:For six different golgins, the capture of carriers is mediated by a short region at the N-terminus of the protein. There appear to be at least four different types of motif, consistent with specific golgins capturing specific classes of carrier and implying the existence of distinct receptors present on each of these different carrier classes.


BMC Biol


BMC biology


Wong M,Gillingham AK,Munro S




Has Abstract


2017-01-26 00:00:00












  • Quality prevails over identity in the sexually selected vocalisations of an ageing mammal.

    abstract:BACKGROUND:Male sexually selected vocalisations generally contain both individuality and quality cues that are crucial in intra- as well as inter-sexual communication. As individuality is a fixed feature whereas male phenotypic quality changes with age, individuality and quality cues may be subjected to different selec...

    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Briefer E,Vannoni E,McElligott AG

    更新日期:2010-04-09 00:00:00

  • TIS7 induces transcriptional cascade of methylosome components required for muscle differentiation.

    abstract:BACKGROUND:TPA Induced Sequence 7 acts as a transcriptional co-regulator controlling the expression of genes involved in differentiation of various cell types, including skeletal myoblasts. We and others have shown that TIS7 regulates adult myogenesis through MyoD, one of the essential myogenic regulatory factors. RES...

    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Lammirato A,Patsch K,Feiereisen F,Maly K,Nofziger C,Paulmichl M,Hackl H,Trajanoski Z,Valovka T,Huber LA,Vietor I

    更新日期:2016-10-25 00:00:00

  • Hibernation-like state induced by an opioid peptide protects against experimental stroke.

    abstract:BACKGROUND:Delta opioid peptide [D-ala2,D-leU5]enkephalin (DADLE) induces hibernation in summer ground squirrels, and enhances preservation and survival of isolated or transplanted lungs and hearts. In the present study, we investigated the protective effect of DADLE in the central nervous system. RESULTS:Adult Spragu...

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    pub_type: 杂志文章


    authors: Borlongan CV,Hayashi T,Oeltgen PR,Su TP,Wang Y

    更新日期:2009-06-17 00:00:00

  • Features of the ancestral bilaterian inferred from Platynereis dumerilii ParaHox genes.

    abstract:BACKGROUND:The ParaHox gene cluster is the evolutionary sister to the Hox cluster. Whilst the role of the Hox cluster in patterning the anterior-posterior axis of bilaterian animals is well established, and the organisation of vertebrate Hox clusters is intimately linked to gene regulation, much less is known about the...

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    pub_type: 杂志文章


    authors: Hui JH,Raible F,Korchagina N,Dray N,Samain S,Magdelenat G,Jubin C,Segurens B,Balavoine G,Arendt D,Ferrier DE

    更新日期:2009-07-23 00:00:00

  • AIMTOR, a BRET biosensor for live imaging, reveals subcellular mTOR signaling and dysfunctions.

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    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Bouquier N,Moutin E,Tintignac LA,Reverbel A,Jublanc E,Sinnreich M,Chastagnier Y,Averous J,Fafournoux P,Verpelli C,Boeckers T,Carnac G,Perroy J,Ollendorff V

    更新日期:2020-07-03 00:00:00

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    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Middleton CA,Nongthomba U,Parry K,Sweeney ST,Sparrow JC,Elliott CJ

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  • Spatial-temporal targeting of lung-specific mesenchyme by a Tbx4 enhancer.

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    pub_type: 杂志文章


    authors: Zhang W,Menke DB,Jiang M,Chen H,Warburton D,Turcatel G,Lu CH,Xu W,Luo Y,Shi W

    更新日期:2013-11-13 00:00:00

  • Mapping the human membrane proteome: a majority of the human membrane proteins can be classified according to function and evolutionary origin.

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    pub_type: 杂志文章


    authors: Almén MS,Nordström KJ,Fredriksson R,Schiöth HB

    更新日期:2009-08-13 00:00:00

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    journal_title:BMC biology

    pub_type: 杂志文章,评审


    authors: McHugo GP,Dover MJ,MacHugh DE

    更新日期:2019-12-02 00:00:00

  • Candidate chemoreceptor subfamilies differentially expressed in the chemosensory organs of the mollusc Aplysia.

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    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Cummins SF,Erpenbeck D,Zou Z,Claudianos C,Moroz LL,Nagle GT,Degnan BM

    更新日期:2009-06-04 00:00:00

  • The complete plastid genome sequence of the parasitic green alga Helicosporidium sp. is highly reduced and structured.

    abstract:BACKGROUND:Loss of photosynthesis has occurred independently in several plant and algal lineages, and represents a major metabolic shift with potential consequences for the content and structure of plastid genomes. To investigate such changes, we sequenced the complete plastid genome of the parasitic, non-photosyntheti...

    journal_title:BMC biology

    pub_type: 杂志文章


    authors: de Koning AP,Keeling PJ

    更新日期:2006-04-21 00:00:00

  • Evolving eukaryotes: an interview with Joel Dacks.

    abstract::Joel Dacks is an Associate Professor and Canada Research Chair in Evolutionary Cell Biology at the University of Alberta, a Scientific Associate at the Natural History Museum (London), and the current President of the International Society for Evolutionary Protistology. His research group studies the evolution and div...

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    pub_type: 传,历史文章,面试


    authors: Dacks JB

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    abstract::This review discusses the many roles atomistic computer simulations of macromolecular (for example, protein) receptors and their associated small-molecule ligands can play in drug discovery, including the identification of cryptic or allosteric binding sites, the enhancement of traditional virtual-screening methodolog...

    journal_title:BMC biology

    pub_type: 杂志文章,评审


    authors: Durrant JD,McCammon JA

    更新日期:2011-10-28 00:00:00

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    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Clayton C

    更新日期:2010-05-24 00:00:00

  • Two-component cyclase opsins of green algae are ATP-dependent and light-inhibited guanylyl cyclases.

    abstract:BACKGROUND:The green algae Chlamydomonas reinhardtii and Volvox carteri are important models for studying light perception and response, expressing many different photoreceptors. More than 10 opsins were reported in C. reinhardtii, yet only two-the channelrhodopsins-were functionally characterized. Characterization of ...

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    authors: Tian Y,Gao S,von der Heyde EL,Hallmann A,Nagel G

    更新日期:2018-12-06 00:00:00

  • Evolution of developmental roles of Pax2/5/8 paralogs after independent duplication in urochordate and vertebrate lineages.

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    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Bassham S,Cañestro C,Postlethwait JH

    更新日期:2008-08-22 00:00:00

  • LoxP-FRT Trap (LOFT): a simple and flexible system for conventional and reversible gene targeting.

    abstract:BACKGROUND:Conditional gene knockout (cKO) mediated by the Cre/LoxP system is indispensable for exploring gene functions in mice. However, a major limitation of this method is that gene KO is not reversible. A number of methods have been developed to overcome this, but each method has its own limitations. RESULTS:We d...

    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Chaiyachati BH,Kaundal RK,Zhao J,Wu J,Flavell R,Chi T

    更新日期:2012-11-30 00:00:00

  • Parkinson's disease-linked Parkin mutations impair glutamatergic signaling in hippocampal neurons.

    abstract:BACKGROUND:Parkinson's disease (PD)-associated E3 ubiquitin ligase Parkin is enriched at glutamatergic synapses, where it ubiquitinates multiple substrates, suggesting that its mutation/loss-of-function could contribute to the etiology of PD by disrupting excitatory neurotransmission. Here, we evaluate the impact of fo...

    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Zhu M,Cortese GP,Waites CL

    更新日期:2018-09-10 00:00:00

  • OptiMouse: a comprehensive open source program for reliable detection and analysis of mouse body and nose positions.

    abstract:BACKGROUND:Accurate determination of mouse positions from video data is crucial for various types of behavioral analyses. While detection of body positions is straightforward, the correct identification of nose positions, usually more informative, is far more challenging. The difficulty is largely due to variability in...

    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Ben-Shaul Y

    更新日期:2017-05-15 00:00:00

  • Gene-environment and protein-degradation signatures characterize genomic and phenotypic diversity in wild Caenorhabditis elegans populations.

    abstract:BACKGROUND:Analyzing and understanding the relationship between genotypes and phenotypes is at the heart of genetics. Research on the nematode Caenorhabditis elegans has been instrumental for unraveling genotype-phenotype relations, and has important implications for understanding the biology of mammals, but almost all...

    journal_title:BMC biology

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    authors: Volkers RJ,Snoek LB,Hubar CJ,Coopman R,Chen W,Yang W,Sterken MG,Schulenburg H,Braeckman BP,Kammenga JE

    更新日期:2013-08-19 00:00:00

  • Concurrent detection of autolysosome formation and lysosomal degradation by flow cytometry in a high-content screen for inducers of autophagy.

    abstract:BACKGROUND:Autophagy mediates lysosomal degradation of cytosolic components. Recent work has associated autophagic dysfunction with pathologies, including cancer and cardiovascular disease. To date, the identification of clinically-applicable drugs that modulate autophagy has been hampered by the lack of standardized a...

    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Hundeshagen P,Hamacher-Brady A,Eils R,Brady NR

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  • The replication machinery of LUCA: common origin of DNA replication and transcription.

    abstract::Origin of DNA replication is an enigma because the replicative DNA polymerases (DNAPs) are not homologous among the three domains of life, Bacteria, Archaea, and Eukarya. The homology between the archaeal replicative DNAP (PolD) and the large subunits of the universal RNA polymerase (RNAP) responsible for transcriptio...

    journal_title:BMC biology

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    authors: Koonin EV,Krupovic M,Ishino S,Ishino Y

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  • Tales of significance.

    abstract::In this experiment, the authors were interested in testing the effect of a small molecule inhibitor on the ratio of males and females in the offspring of their model Dipteran species. The authors report that in a wild-type population, ~50 % of offspring are male. They then test the effect of treating females with the ...

    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Bell G

    更新日期:2016-06-23 00:00:00

  • S28 peptidases: lessons from a seemingly 'dysfunctional' family of two.

    abstract::A recent paper in BMC Structural Biology reports the crystal structure of human prolylcarboxypeptidase (PRCP), one of the two members of the S28 peptidase family. Comparison of the substrate-binding site of PRCP with that of its family partner, dipeptidyl dipeptidase 7 (DPP7), helps to explain the different enzymatic ...

    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Kozarich JW

    更新日期:2010-06-28 00:00:00

  • Comparative analysis of protein coding sequences from human, mouse and the domesticated pig.

    abstract:BACKGROUND:The availability of abundant sequence data from key model organisms has made large scale studies of molecular evolution an exciting possibility. Here we use full length cDNA alignments comprising more than 700,000 nucleotides from human, mouse, pig and the Japanese pufferfish Fugu rubrices in order to invest...

    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Jørgensen FG,Hobolth A,Hornshøj H,Bendixen C,Fredholm M,Schierup MH

    更新日期:2005-01-28 00:00:00

  • Generation of an integrated Hieracium genomic and transcriptomic resource enables exploration of small RNA pathways during apomixis initiation.

    abstract:BACKGROUND:Application of apomixis, or asexual seed formation, in crop breeding would allow rapid fixation of complex traits, economizing improved crop delivery. Identification of apomixis genes is confounded by the polyploid nature, high genome complexity and lack of genomic sequence integration with reproductive tiss...

    journal_title:BMC biology

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    authors: Rabiger DS,Taylor JM,Spriggs A,Hand ML,Henderson ST,Johnson SD,Oelkers K,Hrmova M,Saito K,Suzuki G,Mukai Y,Carroll BJ,Koltunow AM

    更新日期:2016-10-06 00:00:00

  • Negative density-distribution relationship in butterflies.

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    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Päivinen J,Grapputo A,Kaitala V,Komonen A,Kotiaho JS,Saarinen K,Wahlberg N

    更新日期:2005-03-01 00:00:00

  • Origins of cellular geometry.

    abstract::Cells are highly complex and orderly machines, with defined shapes and a startling variety of internal organizations. Complex geometry is a feature of both free-living unicellular organisms and cells inside multicellular animals. Where does the geometry of a cell come from? Many of the same questions that arise in dev...

    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Marshall WF

    更新日期:2011-08-31 00:00:00

  • An automated aquatic rack system for rearing marine invertebrates.

    abstract:BACKGROUND:One hundred years ago, marine organisms were the dominant systems for the study of developmental biology. The challenges in rearing these organisms outside of a marine setting ultimately contributed to a shift towards work on a smaller number of so-called model systems. Those animals are typically non-marine...

    journal_title:BMC biology

    pub_type: 杂志文章


    authors: Henry JQ,Lesoway MP,Perry KJ

    更新日期:2020-05-04 00:00:00

  • Lateral gene transfer between prokaryotes and multicellular eukaryotes: ongoing and significant?

    abstract::The expansion of genome sequencing projects has produced accumulating evidence for lateral transfer of genes between prokaryotic and eukaryotic genomes. However, it remains controversial whether these genes are of functional importance in their recipient host. Nikoh and Nakabachi, in a recent paper in BMC Biology, tak...

    journal_title:BMC biology

    pub_type: 评论,杂志文章


    authors: Ros VI,Hurst GD

    更新日期:2009-05-05 00:00:00