Identification of a tissue kallikrein in human polymorphonuclear leucocytes.


:We have identified a tissue kallikrein in polymorphonuclear (PMN) leucocytes of normal human blood and bone marrow by immunocytochemistry, radioimmunoassay and enzymology. Immunoreactive tissue kallikrein was visualized in the mature neutrophil leucocytes and in immature forms such as metamyelocytes and myelocytes. No tissue kallikrein was detected in eosinophil leucocytes, lymphocytes, macrophages, megakaryocytes and platelets. So far, we have failed to observe immunoreactivity to tissue kallikrein in basophils. The presence of tissue kallikrein in extracts prepared from PMN leucocytes isolated from peripheral blood was demonstrated by immunodiffusion, dot-blotting and by radioimmunoassay. The kininogenase and amidase activity of the extracts resembled that of tissue kallikrein in being resistant to soya bean trypsin inhibitor and sensitive to trasylol. The amidase activity attributable to tissue kallikrein was completely inhibited by specific antisera.


Br J Haematol


Figueroa CD,MacIver AG,Bhoola KD




Has Abstract


1989-07-01 00:00:00












  • Association between hyperflexibility of the thumb and an unexplained bleeding tendency: is it a rule of thumb?

    abstract::A bleeding tendency manifested by petechiae and ecchymoses is one of the most common causes for referral of patients to haematology clinics. Vessel wall pathology is not usually considered to be a cause for deranged haemostasis, although coexistence of increased capillary fragility and joint hypermobility have been re...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Kaplinsky C,Kenet G,Seligsohn U,Rechavi G

    更新日期:1998-05-01 00:00:00

  • Efficacy and safety of romiplostim in refractory aplastic anaemia: a Phase II/III, multicentre, open-label study.

    abstract::A previous dose-finding study has suggested that romiplostim is effective in patients with refractory aplastic anaemia (AA) and 10 µg/kg once weekly was recommended as a starting dose. In this Phase II/III, multicentre, open-label study, romiplostim was administered subcutaneously at a fixed dose of 10 µg/kg once week...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Jang JH,Tomiyama Y,Miyazaki K,Nagafuji K,Usuki K,Uoshima N,Fujisaki T,Kosugi H,Matsumura I,Sasaki K,Kizaki M,Sawa M,Hidaka M,Kobayashi N,Ichikawa S,Yonemura Y,Enokitani K,Matsuda A,Ozawa K,Mitani K,Lee JW,Nakao

    更新日期:2021-01-01 00:00:00

  • A new Turkish type of beta-thalassaemia major with homozygosity for two non-consecutive 7.6 kb deletions of the psi beta and beta genes and an intact delta gene.

    abstract::In a 2.5-month-old infant with beta-thalassaemia major, DNA analysis of the gamma-beta region revealed homozygosity for two large deletions removing the entire psi beta and beta regions including their 5' promoter regions but leaving the delta gene intact. The downstream deletion was predicted to be 7.6 kb in length e...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Oner C,Oner R,Gürgey A,Altay C

    更新日期:1995-02-01 00:00:00

  • High expression of MCL1 gene related to vascular endothelial growth factor is associated with poor outcome in non-Hodgkin's lymphoma.

    abstract::We evaluated the level of MCL1 gene expression using quantitative reverse transcription polymerase chain reaction in lymph nodes of patients with non-Hodgkin lymphoma (NHL). MCL1 expression in patients in complete remission (CR) was significantly lower than in patients with progressive disease (PD, P = 0.0043). The di...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Kuramoto K,Sakai A,Shigemasa K,Takimoto Y,Asaoku H,Tsujimoto T,Oda K,Kimura A,Uesaka T,Watanabe H,Katoh O

    更新日期:2002-01-01 00:00:00

  • Stomatocytic haemolysis and macrothrombocytopenia (Mediterranean stomatocytosis/macrothrombocytopenia) is the haematological presentation of phytosterolaemia.

    abstract::Phytosterolaemia (sitosterolaemia) is a recessively inherited metabolic condition in which the absorption of both cholesterol and plant-derived cholesterol-like molecules at the gut is unselective and unrestricted. In haematology, Mediterranean stomatocytosis or Mediterranean macrothrombocytopenia is a poorly understo...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Rees DC,Iolascon A,Carella M,O'marcaigh AS,Kendra JR,Jowitt SN,Wales JK,Vora A,Makris M,Manning N,Nicolaou A,Fisher J,Mann A,Machin SJ,Clayton PT,Gasparini P,Stewart GW

    更新日期:2005-07-01 00:00:00

  • Patient self-testing is a reliable and acceptable alternative to laboratory INR monitoring.

    abstract::SAn ageing population and the continuing expansion of clinical indications for coumadin therapy have increased pressure on hospital anticoagulant clinics. One solution is patient self-testing (PST) of the international normalized ratio (INR) using capillary blood samples on point-of-care coagulation monitors at home. ...

    journal_title:British journal of haematology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Gardiner C,Williams K,Mackie IJ,Machin SJ,Cohen H

    更新日期:2005-01-01 00:00:00

  • Overexpression of the VAV proto-oncogene product is associated with B-cell chronic lymphocytic leukaemia displaying loss on 13q.

    abstract::The expression of the VAV proto-oncogene in 57 patients with chronic myeloproliferative disease (CMD), B-cell acute lymphoblastic leukaemia (B-ALL) and B-cell non-Hodgkin Lymphoma (B-NHL), and 61 with B-cell chronic lymphocytic leukaemia (B-CLL) was analysed. VAV overexpression was observed in 19.5% of cases and 81% o...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Prieto-Sánchez RM,Hernández JA,García JL,Gutiérrez NC,San Miguel J,Bustelo XR,Hernández JM

    更新日期:2006-06-01 00:00:00

  • Thrombopoietin in Plasmodium falciparum malaria.

    abstract::Thrombopoietin (TPO) is the key growth factor for platelet production and is elevated in states of platelet depletion. As thrombocytopenia is a common finding in malaria, we analysed TPO regulation before, during and after antimalarial treatment. Before treatment, TPO serum levels were significantly higher in patients...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Kreil A,Wenisch C,Brittenham G,Looareesuwan S,Peck-Radosavljevic M

    更新日期:2000-06-01 00:00:00

  • Myelodysplastic syndrome with karyotype abnormality is associated with elevated F-cell production.

    abstract::A sensitive F-cell assay has been used to examine the production of fetal haemoglobin (Hb F) in a group of 77 adult patients with myelodysplastic syndrome (MDS), and a control group composed of 100 normal blood donors. Although the mean F-cell percentage in the MDS group (6.0%) is not statistically different from that...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Craig JE,Sampietro M,Oscier DG,Contreras M,Thein S

    更新日期:1996-06-01 00:00:00

  • Characterization of deletions of chromosome 7 short arm occurring as primary karyotypic anomaly in acute myeloid leukaemia.

    abstract::Three patients with an acute myeloid leukaemia (AML) showed a deletion of the short arm of chromosome 7 with loss of the deleted material. The 7p- anomaly originated from either a terminal or an interstitial deletion and it represented the only karyotypic aberration in all the three cases. According to the clinical, m...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Mecucci C,Van Orshoven A,Boogaerts M,Michaux JL,Van den Berghe H

    更新日期:1989-01-01 00:00:00

  • Induction of Fc gamma R-III (CD16) expression on neutrophils affected by paroxysmal nocturnal haemoglobinuria by administration of granulocyte colony-stimulating factor.

    abstract::The inducibility of glycosyl-phosphatidylinositol (GPI)-anchored proteins on affected paroxysmal nocturnal haemoglobinuria (PNH) neutrophils (PMN) after both in vitro and in vivo stimulation was investigated. Fc gamma R-III (CD16), decay-accelerating factor (DAF/CD55) and 20 kD homologous restriction factor (HRF20/CD5...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Ninomiya H,Muraki Y,Shibuya K,Nagasawa T,Abe T

    更新日期:1993-07-01 00:00:00

  • Reactive perivascular T-cell infiltrate predicts survival in primary central nervous system B-cell lymphomas.

    abstract::Well-established histopathological prognostic factors are lacking in primary central nervous system (CNS) lymphomas (PCNSL). The present study investigated the presence and prognostic role of tumour necrosis (TN) and reactive perivascular T-cell infiltrate (RPVI), defined as a rim of small reactive T-lymphocytes occur...

    journal_title:British journal of haematology

    pub_type: 杂志文章,多中心研究


    authors: Ponzoni M,Berger F,Chassagne-Clement C,Tinguely M,Jouvet A,Ferreri AJ,Dell'Oro S,Terreni MR,Doglioni C,Weis J,Cerati M,Milani M,Iuzzolino P,Motta T,Carbone A,Pedrinis E,Sanchez J,Blay JY,Reni M,Conconi A,Bertoni F

    更新日期:2007-08-01 00:00:00

  • Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase.

    abstract::To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years; parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Mihara K,Imai C,Coustan-Smith E,Dome JS,Dominici M,Vanin E,Campana D

    更新日期:2003-03-01 00:00:00

  • The C677T MTHFR gene mutation is not predictive of risk for recurrent fetal loss.

    abstract::We have investigated the potency of the C677T mutation in the methylene tetrahydrofolate reductase (MTHFR) gene as a genetic risk factor in women with a history of early (12 weeks gestation) recurrent miscarriage (three or more consecutive pregnancy losses). Fifty-seven of the tota...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Holmes ZR,Regan L,Chilcott I,Cohen H

    更新日期:1999-04-01 00:00:00

  • PI3K/AKT pathway activation in acute myeloid leukaemias is not associated with AKT1 pleckstrin homology domain mutation.

    abstract::Despite its' central role, the precise mechanisms of the phosphoinositide 3-kinase/Akt (PI3K)/Akt pathway activation in acute myeloid leukaemia (AML) have not been elucidated. Recently, a recurrent novel AKT1 pleckstrin homology domain (PHD) mutation leading to membrane translocation, constitutive AKT activation and l...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Tibes R,Kornblau SM,Qiu Y,Mousses SM,Robbins C,Moses T,Carpten JD

    更新日期:2008-02-01 00:00:00

  • Preliminary experience of allogeneic stem cell transplantation for lymphoproliferative disorders using BEAM-CAMPATH conditioning: an effective regimen with low procedure-related toxicity.

    abstract::Autologous transplantation has an established role in the treatment of lymphoproliferative disorders, but allogeneic transplantation remains controversial. In an attempt to reduce the high procedure-related mortality reported with allografting in lymphoma, we have used BEAM (BCNU, etoposide, cytarabine and melphalan),...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Cull GM,Haynes AP,Byrne JL,Carter GI,Miflin G,Rebello P,Hale G,Waldmann H,Russell NH

    更新日期:2000-03-01 00:00:00

  • A five-year follow-up of untreated patients with chronic lymphocytic leukaemia treated with ofatumumab and chlorambucil: final analysis of the Complement 1 phase 3 trial.

    abstract::The Complement 1 trial investigated the efficacy and safety of ofatumumab + chlorambucil with chlorambucil monotherapy in patients with previously untreated chronic lymphocytic leukaemia (CLL). On long-term follow-up in the chemoimmunotherapy arm vs. the chemotherapy arm there was an estimated 12% (not significant) an...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Offner F,Robak T,Janssens A,Govind Babu K,Kloczko J,Grosicki S,Mayer J,Panagiotidis P,Schuh A,Pettitt A,Montillo M,Werner O,Vincent G,Khanna S,Hillmen P

    更新日期:2020-09-01 00:00:00

  • Systemic inflammation increases shear stress-induced platelet plug formation measured by the PFA-100.

    abstract::The PFA-100 measures platelet plug formation under shear stress and is strongly dependent on von Willebrand Factor (VWF) levels in plasma. We therefore hypothesized that elevated VWF levels, possibly as a result of acute inflammation, adversely influence PFA-100 results. Healthy volunteers received either 2 ng/kg endo...

    journal_title:British journal of haematology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Homoncik M,Blann AD,Hollenstein U,Pernerstorfer T,Eichler HG,Jilma B

    更新日期:2000-12-01 00:00:00

  • Advances in the understanding of myeloma bone disease and tumour growth.

    abstract::Advances in multiple myeloma support the notion that the associated bone disease, characterized by increased osteoclastogenesis and suppressed osteoblastogenesis, is both a consequence and necessity of tumour progression. Osteoblastogenesis is suppressed by secreted inhibitors and dysregulation of cell-surface 'coupli...

    journal_title:British journal of haematology

    pub_type: 杂志文章,评审


    authors: Yaccoby S

    更新日期:2010-05-01 00:00:00

  • Iron metabolism in murine erythroleukaemic cells.

    abstract::In an attempt to develop a model system for analysing iron metabolism in a relatively homogeneous population of early red cell precursors, the intracellular distribution of 59Fe was examined in Friend murine erythroleukaemic cells after induction of haemoglobin synthesis with dimethylsulphoxide. After incubation of th...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Peto TE,Rutherford TR,Thompson JL,Weatherall DJ

    更新日期:1983-08-01 00:00:00

  • Hybridoma antibodies to human von Willebrand factor. I. Characterization of seven clones.

    abstract::Twenty-seven stable subclones of seven independent cellular hybrids producing murine monoclonal antibodies to human von Willebrand factor (vWF) have been established. The specificity of the hybridoma antibodies for vWF has been substantiated by a variety of methods including binding to highly purified vWF, absence of ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Meyer D,Zimmerman TS,Obert B,Edgington TS

    更新日期:1984-08-01 00:00:00

  • Single amino acid mutation of Fc gamma receptor is associated with the development of heparin-induced thrombocytopenia.

    abstract::Heparin-induced thrombocytopenia (HIT) is mediated by a heparin-dependent antibody/platelet factor 4/heparin complex binding to platelets via the Fc gamma receptor (type IIA). A single base polymorphism at position 131 of Fc gamma RIIA changes the native arginine to histidine. In the presence of murine monoclonal IgG1...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Burgess JK,Lindeman R,Chesterman CN,Chong BH

    更新日期:1995-11-01 00:00:00

  • A phase II study of cyclophosphamide, etoposide, vincristine and prednisone (CEOP) Alternating with Pralatrexate (P) as front line therapy for patients with peripheral T-cell lymphoma (PTCL): final results from the T- cell consortium trial.

    abstract::Peripheral T-cell lymphomas (PTCL) have suboptimal outcomes using conventional CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone) chemotherapy. The anti-folate pralatrexate, the first drug approved for patients with relapsed/refractory PTCL, provided a rationale to incorporate it into the front-line setting...

    journal_title:British journal of haematology

    pub_type: 杂志文章,多中心研究


    authors: Advani RH,Ansell SM,Lechowicz MJ,Beaven AW,Loberiza F,Carson KR,Evens AM,Foss F,Horwitz S,Pro B,Pinter-Brown LC,Smith SM,Shustov AR,Savage KJ,Vose JM

    更新日期:2016-02-01 00:00:00

  • Similar burden of type 2 diabetes among adult patients with sickle cell disease relative to African Americans in the U.S. population: a six-year population-based cohort analysis.

    abstract::Conflicting evidence exists on the epidemiology of type 2 diabetes mellitus (T2DM) among patients with sickle cell disease (SCD). This study measured the prevalence, incidence and clinical outcomes associated with T2DM in a large US population of commercially-insured adults aged ≥20 years with SCD between 2009 and 201...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Zhou J,Han J,Nutescu EA,Galanter WL,Walton SM,Gordeuk VR,Saraf SL,Calip GS

    更新日期:2019-04-01 00:00:00

  • Diagnosis of Hodgkin lymphoma in the modern era.

    abstract::The Hodgkin lymphomas are a family of unique lymphoma subtypes, in which the nature of the neoplastic cell was enigmatic for many years. Much of the mystery has been solved, with all forms now considered to be of B-cell origin, in most cases of germinal centre derivation. Today we recognize Hodgkin lymphoma as an epon...

    journal_title:British journal of haematology

    pub_type: 杂志文章,评审


    authors: Wang HW,Balakrishna JP,Pittaluga S,Jaffe ES

    更新日期:2019-01-01 00:00:00

  • Transplant results in adults with Fanconi anaemia.

    abstract::The outcomes of adult patients transplanted for Fanconi anaemia (FA) have not been well described. We retrospectively analysed 199 adult patients with FA transplanted between 1991 and 2014. Patients were a median of 16 years of age when diagnosed with FA, and underwent transplantation at a median age of 23 years. Time...

    journal_title:British journal of haematology

    pub_type: 杂志文章,多中心研究


    authors: Bierings M,Bonfim C,Peffault De Latour R,Aljurf M,Mehta PA,Knol C,Boulad F,Tbakhi A,Esquirol A,McQuaker G,Sucak GA,Othman TB,Halkes CJM,Carpenter B,Niederwieser D,Zecca M,Kröger N,Michallet M,Risitano AM,Ehninger G

    更新日期:2018-01-01 00:00:00

  • A phase 2 trial of high dose lenalidomide in patients with relapsed/refractory higher-risk myelodysplastic syndromes and acute myeloid leukaemia with trilineage dysplasia.

    abstract::Limited therapies exist for patients with refractory and relapsed (RR) higher-risk myelodysplastic syndromes (HR-MDS) and acute myeloid leukaemia with trilineage dysplasia (AML-TD). High dose (HD) lenalidomide (50 mg) has activity as frontline therapy in elderly AML but there is limited data in the RR setting. This ph...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Zeidan AM,Smith BD,Carraway HE,Gojo I,DeZern A,Gore SD

    更新日期:2017-01-01 00:00:00

  • Studies of oral neutrophil levels in patients receiving G-CSF after autologous marrow transplantation.

    abstract::Patients are at risk of mucositis and infections in the oral cavity during the neutropenic period after chemotherapy, which are significant causes of morbidity. In phase I/II studies with the haemopoietic growth factor granulocyte colony stimulating factor (G-CSF), a reduction in post-chemotherapy mucositis has been o...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Lieschke GJ,Ramenghi U,O'Connor MP,Sheridan W,Szer J,Morstyn G

    更新日期:1992-11-01 00:00:00

  • Combination of cytokines: current status and future prospects.

    abstract::Clinical trials with individual cytokines and extensive in vitro studies have provided the basis for the in vivo use of these molecules in combination. Animal models, with haemopoietic growth factors as well as preliminary studies in humans--as shown by our studies with the sequential use of IL-3 and GM-CSF in patient...

    journal_title:British journal of haematology

    pub_type: 杂志文章,评审


    authors: Kanz L,Brugger W,Bross K,Mertelsmann R

    更新日期:1991-10-01 00:00:00

  • Oxidative stress is involved in hydroxyurea-induced erythroid differentiation.

    abstract::Hydroxyurea (HU), an inhibitor of DNA synthesis, can also induce haemoglobinization in certain erythroid cell lines. In this study, we report that intracellular peroxides levels were increased in HU-treated murine erythroleukaemia (MEL) cells and that l-acetyl-N-cysteine (LNAC), a potent reducing reagent, had a signif...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Nagai T,Tarumoto T,Miyoshi T,Ohmine K,Muroi K,Komatsu N,Sassa S,Ozawa K

    更新日期:2003-05-01 00:00:00